product summary
Loading...
company name :
DIMA Biotechnology
product type :
protein
product name :
Human SIRPα Protein, hFc-His Tag
catalog :
PME100009
quantity :
10 μg, 100 μg
price :
60 USD, 378 USD
more info or order :
DIMA Biotechnology product webpage
image
image 1 :
DIMA Biotechnology PME100009 image 1
Figure 1. Human SIRPα, hFc-His Tag on SDS-PAGE under reducing condition.
image 2 :
DIMA Biotechnology PME100009 image 2
Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CD47, mFc-His tagged protein (PME100008) can bind its native ligand Human SIRPα, hFc-His tagged protein (PME100009) in a linear range of 3.3-26.37 ng/ml.
image 3 :
DIMA Biotechnology PME100009 image 3
Figure 3. A: Human SIRPα, hFc-His tagged protein without freeze-thaw treatment. B: Human SIRPα, hFc-His tagged protein after one freeze-thaw cycle. C: Human SIRPα, hFc-His tagged protein after three freeze-thaw cycles. D: Human SIRPα, hFc-His tagged protein after five freeze-thaw cycles.
product information
Catalog Number :
PME100009
Product Name :
Human SIRPα Protein, hFc-His Tag
Product Type :
protein
Size :
10 μg, 100 μg
List Price :
60 USD, 378 USD
Product Description :
Recombinant human SIRPα protein with C-terminal human Fc and 6×His tag
Background :
Tyrosine-protein phosphatase non-receptor type substrate 1 (SHPS1) is also known as CD172 antigen-like family member A (CD172a), Macrophage fusion receptor, MyD-1 antigen, Signal-regulatory protein alpha (SIRPA or SIRP alpha) or p84, is a member of the SIRP family, and also belongs to the immunoglobulin superfamily. SIRP alpha is Ubiquitous and highly expressed in brain. SIRPA / CD172a is immunoglobulin-like cell surface receptor for CD47 and acts as docking protein and induces translocation of PTPN6,PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA / SHPS-1 supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment and may play a key role in intracellular signaling during synaptogenesis and in synaptic function By similarity. SIRPA / MyD1 involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin and mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.
Purity :
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Buffer :
Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 23 % trehalose is added as protectants before lyophilization.
Uses :
Research use only
Application Summary :
Immunogen,WB,Elisa
Storage :
Store at -80°C for 12 months (Avoid repeated freezing and thawing)
more info or order :
DIMA Biotechnology product webpage
company information
DIMA Biotechnology
Wuhan institute of Biotechnology B7
Biolake No.666 Gaoxin Road
Wuhan, Hubei
info@dimabio.com
https://www.dimabio.com
0118615307149771
headquarters: China
DIMA BIOTECH LTD (DIMABIO) focuses exclusively on the pre-clinical research market of Immune Oncology. Currently, we offer functional proteins and recombinant monoclonal antibodies. Our proprietary DimAb™ technology platform can directly clone IgG gene from immunized animal, which is a technology breakthrough in monoclonal antibody development.

All DIMABIOs protein products were manufactured by using mammalian cell expression system. To analyze the function of DIMABIOs proteins, we evaluated them by paired binding test between native ligand-receptor and or the binding capacity to the therapeutic antibodies. For DimAb™ recombinant monoclonal antibodies, we focus mainly on identifying clones with flow and blocking activity.