product summary
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company name :
DIMA Biotechnology
product type :
antibody
product name :
Anti-SIRPα antibody(DM8), Rabbit mAb
catalog :
DME100008
quantity :
10 μl, 100 μl
price :
60 USD, 398 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
DM8
reactivity :
human
application :
immunoprecipitation, flow cytometry
more info or order :
DIMA Biotechnology product webpage
image
image 1 :
DIMA Biotechnology DME100008 image 1
Figure 1. THP-1 cell line were surface stained with Rabbit anti-SIRPα monoclonal antibody 1μg/ml (clone: DM8) and Rabbit IgG isotype control antibody followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.
image 2 :
DIMA Biotechnology DME100008 image 2
Figure 2. A) Flow cytometry data of serially titrated Rabbit anti-SIRPα monoclonal antibody (clone: DM8). The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used. B) Competition assay demonstrating DM8 blockade of CD47 binding to THP-1 cells. IC50=30.88ng/ml.
image 3 :
DIMA Biotechnology DME100008 image 3
Figure 2. A) Flow cytometry data of serially titrated Rabbit anti-SIRPα monoclonal antibody (clone: DM8). The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used. B) Competition assay demonstrating DM8 blockade of CD47 binding to THP-1 cells. IC50=30.88ng/ml.
product information
Catalog Number :
DME100008
Product Name :
Anti-SIRPα antibody(DM8), Rabbit mAb
Product Type :
antibody
Host Species :
Rabbit
Clonality :
Monoclonal
Conjugation :
Unconjugated
Antigen Modification :
no modification
Clone Name :
DM8
Isotype :
Rabbit IgG
Light Chain :
kappa
Size :
10 μl, 100 μl
List Price :
60 USD, 398 USD
SpeciesSummary :
Human
Background :
The protein encoded by this gene is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown to recruit SH2 domain containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. This protein was found to participate in signal transduction mediated by various growth factor receptors. CD47 has been demonstrated to be a ligand for this receptor protein. This gene and its product share very high similarity with several other members of the SIRP family. These related genes are located in close proximity to each other on chromosome 20p13. Multiple alternatively spliced transcript variants have been determined for this gene. [provided by RefSeq, Jul 2008]
Immunogen :
Recombinant human SIRPα (Glu31-Arg370) produced by using human HEK293 cells
Specificity :
ELISA, Flow Cyt, IP, IF
Purity :
Purified from cell culture supernatant by affinity chromatography
Buffer :
50% Glycerol; PBS, pH 7.4; 0.1% BSA
Preservative :
0.1% Procline 300
Packaging :
10ul/100ul
Uses :
Research use only
Application Summary :
Flow Cyt  1/100; IP 1/30
Storage :
Store at -20°C for 12 months (Avoid repeated freezing and thawing)
more info or order :
DIMA Biotechnology product webpage
company information
DIMA Biotechnology
Wuhan institute of Biotechnology B7
Biolake No.666 Gaoxin Road
Wuhan, Hubei
info@dimabio.com
https://www.dimabio.com
0118615307149771
headquarters: China
DIMA BIOTECH LTD (DIMABIO) focuses exclusively on the pre-clinical research market of Immune Oncology. Currently, we offer functional proteins and recombinant monoclonal antibodies. Our proprietary DimAb™ technology platform can directly clone IgG gene from immunized animal, which is a technology breakthrough in monoclonal antibody development.

All DIMABIOs protein products were manufactured by using mammalian cell expression system. To analyze the function of DIMABIOs proteins, we evaluated them by paired binding test between native ligand-receptor and or the binding capacity to the therapeutic antibodies. For DimAb™ recombinant monoclonal antibodies, we focus mainly on identifying clones with flow and blocking activity.