This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Creative BioMart
product type :
protein
product name :
Recombinant Human Topoisomerase (DNA) I, Core Domain
catalog :
TOP1-1195H
product information
Cat :
TOP1-1195H
Product Name :
Recombinant Human Topoisomerase (DNA) I, Core Domain
Brand :
Creative Biomart
gene symbal :
TOP1
gene name :
topoisomerase (DNA) I
Synonyms :
TOP1; topoisomerase (DNA) I; TOPI; DNA topoisomerase 1; OTTHUMP00000031713; DNA topoisomerase I; type I DNA topoisomerase
Product Overview :
Recombinant protein production has become an essential tool for providing the necessary amounts of a protein of interest to either research or therapy by providing adequate amounts of proteins for studying protein structure and function. Moreover, scaled-up facilities produce recombinant vaccines, hormones, antibodies, growth factors, blood components, and enzymes.
Description :
Human DNA Topoisomerase I is the best studied of the DNA topoisomerase family. It catalyzes the relaxation of both positive and negative supercoiled DNAs without the requirement of energy. In addition to DNA replication and transcriptional activation, DNA Topoisomerase I also plays a major role in pre-mRNA splicing, cell cycle, and other gene regulatory pathways during cell growth and development. The core domain expanded from amino acids 215 to 636 is highly conserved and retains DNA binding activity. The substrate specificity of Topo I has been found to nick the DNA with a preference of 5'-(A/T)(G/C)(A/T)T-3'. Camptothecin and its analogs have been tested as potent anticancer compounds by stabilizing Topo I-DNA complex, thereby inhibiting both DNA and RNA synthesis. The core domain of DNA Topoisomerase I protein (residues 197-651) was expressed in baculovirus system and purified by using an affinity column and FPLC chromatography. Purified core domain of Topo I can be used for DNA binding and protein-protein interaction assays. It can be also reconstituted with the C-terminal domain to restore the DNA relaxation activity in vitro.
Source :
Sf9 insect cells
Form :
Liquid. Supplied in 20 mM Tris-HCl, pH 8.0, 100 mM KCl, 0.2 mM EDTA, 1 mM DTT, 20 % glycerol.
Purity :
95% by SDS-PAGE.
Storage :
Quality guaranteed for 12 months store at -80 C. Avoid freeze / thaw cycles.
UniProt ID :
P11387
company information
Creative BioMart
Creative BioMart provides quality recombinant proteins, diagnostic antibodies and antigens, diagnostic enzymes and pharmaceutical enzymes to the research community of biology, clinical research, molecular diagnostics and biopharmaceutical drug development.
We are offering more than 3,000 recombinant proteins, peptides and antibodies. All the products are rigorously tested to meet the most demanding research needs. At the same time, lowest prices in the industry are always guaranteed.
Also, in order to leverage our core competencies and resources, Creative BioMart has formed a large number of corporate partnerships and academic collaborations for product development and distribution. We welcome potential partners and distributors to explore business relationships with Creative BioMart.
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