protein

Recombinant Human SRC, GST-tagged

SRC-191H

Recombinant Human SRC, GST-tagged

Creative Biomart

SRC

v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog

SRC; v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog; ASV; SRC1; c-SRC; p60-Src; proto-oncogene tyrosine-protein kinase Src; proto-oncogene c-Src; tyrosine kinase pp60c-src; tyrosine-protein kinase SRC-1; protooncogene SRC, Rous sarcoma; EC 2.7.10.2

Recombinant protein production has become an essential tool for providing the necessary amounts of a protein of interest to either research or therapy by providing adequate amounts of proteins for studying protein structure and function. Moreover, scaled-up facilities produce recombinant vaccines, hormones, antibodies, growth factors, blood components, and enzymes.

Src (p60c-src) is a 60 kDa cytoplasmic tyrosine kinase with functions in the regulation of cell division, cell-matrix adhesion, and cell-cellcell adhesion. Src-GST family tyrosine kinases participate in signal transduction of different classes of membrane receptors, including receptor tyrosine kinases and G-protein coupeled receptors. The SrcGST has been generated by fusing GST N-terminal to human c-Src allowing expression in E. coli and purification by affinity chromatography with GSHbeads. The preparation is 90% homogenous in SDS-PAGE and has a specific activity of 3000 U/mg (1 U is defined as transfer of 1 pMol phosphate to the substrate peptide AAEEEIYGEFEAKKKK per minute at 30 C). The fusion protein has been successfully used to label phosphatase substrates. The GST-Tag facilitates the protein's application in typical GST pull-down assays.

E coli

Liquid. Supplied in 50 mM Tris-HCl pH 8.0, 100 mM NaCl and 1 mM DTT.

≥90% by SDS-PAGE.

Quality guaranteed for 12 months store at -80 C. Avoid freeze / thaw cycles.

P12931