protein

Recombinant Human MMP14(Membrane-inserted), Catalytic Domain

MMP14-160H

Recombinant Human MMP14(Membrane-inserted), Catalytic Domain

Creative Biomart

MMP14

matrix metallopeptidase 14 (membrane-inserted)

MMP14; matrix metallopeptidase 14 (membrane-inserted); MMP-14; MMP-X1; MT-MMP; MT1MMP; MTMMP1; WNCHRS; MT1-MMP; MT-MMP 1; matrix metalloproteinase-14; membrane type 1 metalloprotease; membrane-type-1 matrix metalloproteinase;

Recombinant Human matrix metalloproteinase-14 (MMP-14, Membrane-Type Matrix Metalloproteinase1, MT1-MMP) cloned from human cDNA was expressed in E.coli . The enzyme consists of the catalytic domain of human MMP-14 (residues 114-290 swissprot accession P50281) with a C-term purification tag. The protein has been mutated to increase the stability. The catalytic activity rates are not affected by the mutation. The C-terminal tag is cleaved during purification. MW=21.3kDa.

Recombinant Human matrix metalloproteinase-14 (MMP-14, Membrane-Type Matrix Metalloproteinase1, MT1-MMP) cloned from human cDNA was expressed in E.coli . The enzyme consists of the catalytic domain of human MMP-14 (residues 114-290 swissprot accession P50281) with a C-term purification tag. The protein has been mutated to increase the stability. The catalytic activity rates are not affected by the mutation. The C-terminal tag is cleaved during purification. MW=21.3kDa.Matrix metalloproteinase-14 is an enzyme that in humans is encoded by the MMP14 gene. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP"s are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion.

Escherichia Coli

95% by SDS-PAGE. The enzyme was observed as a single band migrating at a molecular weight of 20kDa.

-80 C. The enzyme is stable at -20 C for at least 1 week. After initial defrost, aliquot enzyme into individual tubes and refreeze at -80 C. Avoid repeated freeze/defrost cycles.

P50281