protein

Recombinant Human CSF2, His-tagged

CSF2-143H

Recombinant Human CSF2, His-tagged

Creative Biomart

CSF2

colony stimulating factor 2 (granulocyte-macrophage)

CSF2; colony stimulating factor 2 (granulocyte-macrophage); GMCSF; granulocyte-macrophage colony-stimulating factor; CSF; molgramostin; sargramostim;

rHuman GM-CSF is a glycosylated polypeptide chain containing 127 amino acids (18-144 aa CSF2_HUMAN P04141 ), fused to 10 His tag at N-terminal. rHuman GM-CSF migrate as a broad band between 15 and 25 kDa due to post-translation modification, in particular glycosylation. Human recombinant protein expressed in Nicotiana benthamiana. Recombinant human Granulocyte-macrophage colony-stimulating factor (GM-CSF) contains a 10-His-tag at the N-terminal end, is produced by transient expression in non-transgenic plants and is purified by sequential chromatography (FPLC). This product contains no animal–derived components or impurities. Animal free product.

rHuman GM-CSF is a glycosylated polypeptide chain containing 127 amino acids (18-144 aa CSF2_HUMAN P04141 ), fused to 10 His tag at N-terminal. rHuman GM-CSF migrate as a broad band between 15 and 25 kDa due to post-translation modification, in particular glycosylation. Human recombinant protein expressed in Nicotiana benthamiana. Recombinant human Granulocyte-macrophage colony-stimulating factor (GM-CSF) contains a 10-His-tag at the N-terminal end, is produced by transient expression in non-transgenic plants and is purified by sequential chromatography (FPLC). This product contains no animal–derived components or impurities. Animal free product.GMCSF is a cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. It′s involved in differentiation of dendritic cells and is a key factor in differentiation pathways leading form stem cells. GMCSF is produced by several cell types as monocytes, fibroblasts, endothelial cells and T- Lymphocytes in response to a number of inflammatory mediators present in the hemopoietic environment and peripheral site of inflammation. Human GMCF is an important therapeutic cytokine used in the treatment of myeloid leukemia, neutropenia and aplastic anemia and it could become interesting in the treatment following bone marrow transplantation. It performs its biological activity by binding to a specific receptor complex which is composed of a cytokine-specific alpha chain and beta chain, shared with the receptors for interleukin-3 and interleukin-5. GMCSR has been identified to mediate the activation of Jak-Stat and MAPK pathways.

Nicotiana benthamiana

Recombinant human GM-CSF is lyophilized from 10 mM PBS buffer pH 7.6 and 0.2 M NaCl.

>97% by SDS-PAGE gel

This lyophilized preparation is stable at 2-8o C for short term, long storage it should be kept at -20oC. Reconstituted rh GM-CSF should be stored in working aliquots at –20°C. Repeated freezing and thawing is not recommended.

P04141