protein

Recombinant Human RAD51

RAD51-1121H

Recombinant Human RAD51

Creative Biomart

RAD51

RAD51 recombinase

RAD51; RAD51 recombinase; RECA; BRCC5; MRMV2; HRAD51; RAD51A; HsRad51; HsT16930; DNA repair protein RAD51 homolog 1; RAD51 homolog A; RecA-like protein; recombination protein A; RecA, E. coli, homolog of; BRCA1/BRCA2-containing complex, subunit 5;

Recombinant protein production has become an essential tool for providing the necessary amounts of a protein of interest to either research or therapy by providing adequate amounts of proteins for studying protein structure and function. Moreover, scaled-up facilities produce recombinant vaccines, hormones, antibodies, growth factors, blood components, and enzymes.

Properly controlled recombination between homologous DNA molecules (Homologous Recombination -HR) is essential for the maintenance of genome stability and for the prevention of tumorigenesis. Rad51 is a mammalian homologue of yeast RAD51 and bacterial RecA and, like its counterparts, plays a central role in HR. Rad51 coats ssDNA to form a nucleoprotein filament that invades and pairs with a homologous region in duplex DNA. It can then activate strand exchange to generate a crossover between the juxtaposed DNA molecules. In addition to Rad51, these steps require the coordinated action of a number of other homologous-recombination proteins, including the RP-A protein, which binds single-stranded DNA, Rad52, which can bind DNA ends, anneal complementary single-stranded DNA molecules and enhance the specificity of RAD51, and a number of Rad51 paralogs. The tumour-suppressor proteins BRCA1 and BRCA2 colocalize with RAD51 in DNA-damage-induced nulear foci. BRCA2 has been shown to interact directly with RAD51 and thus plays a direct role in repair by HR, through control of the availability and function of the central mediator, Rad51. Recombinant RAD51 was expressed in a strain of E.coli and purified by an affinity column in combination with FPLC chromatography.

Ecoli

Liquid. Supplied in 20 mM Tris-HCl pH 8.0, 100 mM KCl, 0.2 mM EDTA, 1 mM DTT and 20% glycerol.

≥95% by SDS-PAGE.

Quality guaranteed for 12 months store at -80 C. Avoid freeze / thaw cycles.

Q06609