protein

Recombinant Human Mdm2 p53 Binding Protein Homolog, GST-tagged

MDM2-1063H

Recombinant Human Mdm2 p53 Binding Protein Homolog, GST-tagged

Creative Biomart

MDM2

MDM2 oncogene, E3 ubiquitin protein ligase

MDM2; MDM2 oncogene, E3 ubiquitin protein ligase; HDMX; hdm2; ACTFS; E3 ubiquitin-protein ligase Mdm2; oncoprotein Mdm2; Mdm2, p53 E3 ubiquitin protein ligase homolog; double minute 2, human homolog of; p53-binding protein; Mdm2, transformed 3T3 cell double minute 2, p53 binding protein;

Recombinant human MDM2 (202-end) was expressed in E. coli cells using an N-terminal GST tag.

Recombinant human MDM2 (202-end) was expressed in E. coli cells using an N-terminal GST tag.MDM2 is a nuclear phosphoprotein that binds and inhibits transactivation by p53, as part of an autoregulatory negative feedback loop (1). Overexpression of the MDM2 gene product can lead to excessive inactivation of p53 thereby diminishing its tumor suppressor function. The inactivation of p53 is mediated by the E3 ubiquitin ligase activity of MDM2 which targets p53 for proteasomal degradation. MDM2 also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5 (2). Amplification of MDM2 is frequently observed in human sarcomas and this is consistent with the hypothesis that MDM2 binds to p53 which then leads to escape from p53-regulated growth control.

Human

Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 0.25mM DTT, 0.1mM PMSF, 25%glycerol.

75%

0.2ug/ul

Store product at -70 C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Q00987