protein

Recombinant Human LGALS8

LGALS8-3624H

Recombinant Human LGALS8

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LGALS8

lectin, galactoside-binding, soluble, 8

LGALS8; lectin, galactoside-binding, soluble, 8; Gal-8; PCTA1; PCTA-1; Po66-CBP; galectin-8; galectin 8; galectin-8g; Po66 carbohydrate binding protein; po66 carbohydrate-binding protein; prostate carcinoma tumor antigen 1;

Recombinant Human Galectin-8 produced in E. coli is a single, non-glycosylated, polypeptide chain containing 317 amino acids and having a molecular mass of 35.8 kDa.

Recombinant Human Galectin-8 produced in E. coli is a single, non-glycosylated, polypeptide chain containing 317 amino acids and having a molecular mass of 35.8 kDa.Galectins are a family of animal lectins with an affinity for β-galactosides. There are at least 14 members identified. Galectins share similarities in carbohydrate recognition domain (CRD). Galectins are synthesized as cytosolic proteins. Although localized primarily in the cytoplasm and lacking a classical signal peptide, galectins can also be stimulated to secretion by non-classical pathways or alternatively targeted to the nucleus. Galectins are implicated in modulating cell-cell and cell-matrix interactions. Galectin-8 is a tandem-repeat-type member of galectin family, consisting of two CRDs joined by a linker peptide. Galectin-8 is highly expressed in lung carcinomas,certain forms of prostate carcinomas, as well as other tumor cells. It is also known as prostate carcinoma tumor antigen 1 (PCTA1) in human. Galectin-8 binds to a subset of cell surface integrins to modulate ECM-integrin interactions. When immobilized, Galectin-8 promotes cell adhesion by ligation and clustering of cell surface integrin receptors. In contrast, as a soluble ligand, Galectin-8 can inhibit cell adhesion. Human and mouse Galectin-8 share 80% sequence identity.

The protein was lyophilized from a concentrated (1mg/ml) solution in 20mM PBS, pH 7.4.

Greater than 95% as determined by (a) Analysis by RP-HPLC; (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Lyophilized rHuGalectin-8 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution rHuGalectin-8 should be stored at 4 C between 2-7 days and for future use below -18 C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

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