protein

Recombinant Human Fms-related Tyrosine Kinase 1, 5 Domains, Fc-tagged

FLT1-81H

Recombinant Human Fms-related Tyrosine Kinase 1, 5 Domains, Fc-tagged

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FLT1

fms-related tyrosine kinase 1

FLT1; fms-related tyrosine kinase 1; FLT; FLT-1; VEGFR1; VEGFR-1; vascular endothelial growth factor receptor 1; fms-like tyrosine kinase 1; tyrosine-protein kinase FRT; tyrosine-protein kinase receptor FLT; vascular permeability factor receptor; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor);

Recombinant Human Soluble Fms-related Tyrosine Kinase 1 fused with the Fc part of human IgG1 produced in baculovirus is disulfide-linked homodimeric, glycosylated, polypeptide containing 751 amino acids and having a molecular mass of 130 kDa. The soluble receptor protein contains only the first 7 extracellular domains, which contain all the information necessary for binding of VEGF. The VEGFR1 fc/Chimera is purified by proprietary chromatographic techniques.

Recombinant Human Soluble Fms-related Tyrosine Kinase 1 fused with the Fc part of human IgG1 produced in baculovirus is disulfide-linked homodimeric, glycosylated, polypeptide containing 751 amino acids and having a molecular mass of 130 kDa. The soluble receptor protein contains only the first 7 extracellular domains, which contain all the information necessary for binding of VEGF. The VEGFR1 fc/Chimera is purified by proprietary chromatographic techniques.Endothelial cells express three different vascular endothelial growth factor (VEGF) receptors, belonging to the family of receptor tyrosine kinases (RTKs). They are named VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), and VEGFR-3 (Flt-4). Their expression is almost exclusively restricted to endothelial cells, but VEGFR-1 can also be found on monocytes. All VEGF-receptors have seven immunoglobulin-like extracellular domains, a single transmembrane region and an intracellular split tyrosine kinase domain. VEGFR-2 has a lower affinity for VEGF than the Flt-1 receptor, but a higher signalling activity. Mitogenic activity in endothelial cells is mainly mediated by VEGFR-2 leading to their proliferation. Differential splicing of the flt-1 gene leads to the formation of a secreted, soluble variant of VEGFR-1 (sVEGFR-1). No naturally occurring, secreted forms of VEGFR-2 have so far been reported. The binding of VEGF165 to VEGFR-2 is dependent on heparin.

Insect Cells

Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Lyophilized FLT-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C . Upon reconstitution FLT1 should be stored at 4 C between 2-7 days and for future use below -18 C . For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

P17948