ELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2) | Cloud-Clone Corp SEA551Bo product information

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product summary

company name :

Cloud-Clone Corp

product type :

ELISA/assay

product name :

ELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2)

catalog :

SEA551Bo

quantity :

price :

756.00 USD

product information

Product No. :

SEA551Bo

Name :

ELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2)

UOM :

96-well strip plate

Quantity :

Price(USD) :

756.00 USD

Detection range :

15.625-1000pg/mL The standard curve concentrations used for the ELISA’s were 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL, 15.625pg/mL

Application :

ELISA

Organism species :

Bos taurus; Bovine (Cattle)

Sensitivity :

The minimum detectable dose of this kit is typically less than 6.5pg/mL

Sample type :

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Assay length :

4.5hours

Method :

sandwich

Specificity :

This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 2, Basic (FGF2) No significant cross-reactivity or interference between Fibroblast Growth Factor 2, Basic (FGF2) and analogues was observed.

Precision :

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 2, Basic (FGF2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 2, Basic (FGF2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV Inter-Assay: CV

Stability :

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary :

1. Prepare all reagents, samples and standards; 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 o C; 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 o C; 4. Aspirate and wash 3 times; 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 o C; 6. Aspirate and wash 5 times; 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 o C; 8. Add 50uL Stop Solution. Read at 450nm immediately.

Test principle :

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 2, Basic (FGF2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor 2, Basic (FGF2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibroblast Growth Factor 2, Basic (FGF2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Fibroblast Growth Factor 2, Basic (FGF2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.