ELISA Kit for Lipopolysaccharide (LPS) | Cloud-Clone Corp CEB526Ge product information

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product summary

company name :

Cloud-Clone Corp

product type :

ELISA/assay

product name :

ELISA Kit for Lipopolysaccharide (LPS)

catalog :

CEB526Ge

quantity :

96T

price :

711 USD

citations: 1

Reference
Yang A, Inamine T, Hochrath K, Chen P, Wang L, Llorente C, et al. Intestinal fungi contribute to development of alcoholic liver disease. J Clin Invest. 2017;127:2829-2841 pubmed publisher

product information

Product No. :

CEB526Ge

Product Name :

ELISA Kit for Lipopolysaccharide (LPS)

Format :

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range :

12.35-1000ng/mL

Application :

Enzyme-linked immunosorbent assay for Antigen Detection

Price?96T? :

711 USD

Organism species :

Pan-species (General)

Sensitivity :

The minimum detectable dose of this kit is typically less than 4.93ng/mL

Sample type :

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length :

Method :

Competitive Inhibition

Specificity :

This assay has high sensitivity and excellent specificity for detection of Lipopolysaccharide (LPS). No significant cross-reactivity or interference between Lipopolysaccharide (LPS) and analogues was observed.

Precision :

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lipopolysaccharide (LPS) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lipopolysaccharide (LPS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV Inter-Assay: CV

Stability :

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary :

1. Prepare all reagents, samples and standards; 2. Add 50uL standard or sample to each well. And then add 50uL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37 3. Aspirate and wash 3 times; 4. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 5. Aspirate and wash 5 times; 6. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37 7. Add 50uL Stop Solution. Read at 450 nm immediately.

Test principle :

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Lipopolysaccharide (LPS) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Lipopolysaccharide (LPS) and unlabeled Lipopolysaccharide (LPS) (Standards or samples) with the pre-coated antibody specific to Lipopolysaccharide (LPS). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Lipopolysaccharide (LPS) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Lipopolysaccharide (LPS) in the sample.

Alternative Names :

LOS; Lipoglycans; Lipooligosaccharide; Lipo-Oligosaccharide; Endotoxin

Item Name :

Lipopolysaccharide