product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
GSK-3β (3D10) Mouse mAb
catalog :
9832
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
3D10
reactivity :
human, mouse, rat
application :
western blot, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, western blot knockout validation
citations: 30
Published Application/Species/Sample/DilutionReference
  • western blot knockout validation; mouse; loading ...; fig 2a
Amaral A, Perez Nievas B, Siao Tick Chong M, González Martínez A, Argente Escrig H, Rubio Guerra S, et al. Isoform-selective decrease of glycogen synthase kinase-3-beta (GSK-3β) reduces synaptic tau phosphorylation, transcellular spreading, and aggregation. iScience. 2021;24:102058 pubmed publisher
  • western blot; mouse; 1:500-1:2000; loading ...; fig 3c
Chiang S, Braidy N, Maleki S, Lal S, Richardson D, Huang M. Mechanisms of impaired mitochondrial homeostasis and NAD+ metabolism in a model of mitochondrial heart disease exhibiting redox active iron accumulation. Redox Biol. 2021;46:102038 pubmed publisher
  • western blot; rat; 1:1000; loading ...; fig 3c
Correll E, Ramser B, Knott M, McCullumsmith R, McGuire J, Ngwenya L. Deficits in pattern separation and dentate gyrus proliferation after rodent lateral fluid percussion injury. IBRO Neurosci Rep. 2021;10:31-41 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 4e
Woo Y, Kim S, Suh B, Kwak Y, Jung H, Nhung T, et al. Sequential phosphorylation of NDEL1 by the DYRK2-GSK3β complex is critical for neuronal morphogenesis. elife. 2019;8: pubmed publisher
  • immunohistochemistry - paraffin section; human; loading ...; fig 4d
  • western blot; human; loading ...; fig 1d
Zheng C, Wang J, Lin M, Zhang P, Liu L, Lin J, et al. CDK5RAP3 suppresses Wnt/β-catenin signaling by inhibiting AKT phosphorylation in gastric cancer. J Exp Clin Cancer Res. 2018;37:59 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 5a
  • western blot; human; loading ...; fig 1a
Zheng L, Conner S. Glycogen synthase kinase 3β inhibition enhances Notch1 recycling. Mol Biol Cell. 2018;29:389-395 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 2a
Wilkinson B, Li J, Coba M. Synaptic GAP and GEF Complexes Cluster Proteins Essential for GTP Signaling. Sci Rep. 2017;7:5272 pubmed publisher
  • western blot; mouse; loading ...; fig 1f
Hui X, Zhang M, Gu P, Li K, Gao Y, Wu D, et al. Adipocyte SIRT1 controls systemic insulin sensitivity by modulating macrophages in adipose tissue. EMBO Rep. 2017;18:645-657 pubmed publisher
  • western blot; mouse; fig 4
Hinds T, Burns K, Hosick P, McBeth L, Nestor Kalinoski A, Drummond H, et al. Biliverdin Reductase A Attenuates Hepatic Steatosis by Inhibition of Glycogen Synthase Kinase (GSK) 3? Phosphorylation of Serine 73 of Peroxisome Proliferator-activated Receptor (PPAR) ?. J Biol Chem. 2016;291:25179-25191 pubmed
  • western blot; human; 1:2000; loading ...; fig s2b
Sun W, Lee S, Huang X, Liu S, Inayathullah M, Kim K, et al. Attenuation of synaptic toxicity and MARK4/PAR1-mediated Tau phosphorylation by methylene blue for Alzheimer's disease treatment. Sci Rep. 2016;6:34784 pubmed publisher
  • western blot; human; fig 6a
Xing H, Lim Y, Chong J, Lee J, Aarsland D, Ballard C, et al. Increased phosphorylation of collapsin response mediator protein-2 at Thr514 correlates with ?-amyloid burden and synaptic deficits in Lewy body dementias. Mol Brain. 2016;9:84 pubmed publisher
  • western blot; human; fig 6
Bie Q, Sun C, Gong A, Li C, Su Z, Zheng D, et al. Non-tumor tissue derived interleukin-17B activates IL-17RB/AKT/β-catenin pathway to enhance the stemness of gastric cancer. Sci Rep. 2016;6:25447 pubmed publisher
  • western blot; mouse; fig 8a
Young P, Leonard S, Martin D, Findlay J. The effect of retinol binding protein on the proteome of C2C12 muscle cells. Diabetes Metab Res Rev. 2016;32:379-90 pubmed publisher
  • western blot; mouse; 1:2000
Radford H, Moreno J, Verity N, Halliday M, Mallucci G. PERK inhibition prevents tau-mediated neurodegeneration in a mouse model of frontotemporal dementia. Acta Neuropathol. 2015;130:633-42 pubmed publisher
  • western blot; rat; 1:1000; fig 5
Yan G, Wang Q, Hu S, Wang D, Qiao Y, Ma G, et al. Digoxin inhibits PDGF-BB-induced VSMC proliferation and migration through an increase in ILK signaling and attenuates neointima formation following carotid injury. Int J Mol Med. 2015;36:1001-11 pubmed publisher
  • western blot; human; fig 5
Shi C, Zhang M, Tong M, Yang L, Pang L, Chen L, et al. miR-148a is Associated with Obesity and Modulates Adipocyte Differentiation of Mesenchymal Stem Cells through Wnt Signaling. Sci Rep. 2015;5:9930 pubmed publisher
  • western blot; mouse; fig 5
Jackson B, Ivanova I, Dagnino L. An ELMO2-RhoG-ILK network modulates microtubule dynamics. Mol Biol Cell. 2015;26:2712-25 pubmed publisher
  • western blot; human; 1:1000; fig 5f
Riise J, Plath N, Pakkenberg B, Parachikova A. Aberrant Wnt signaling pathway in medial temporal lobe structures of Alzheimer's disease. J Neural Transm (Vienna). 2015;122:1303-18 pubmed publisher
  • western blot; mouse
Fine J, Renner D, Forsberg A, Cameron R, Galick B, Le C, et al. Intranasal deferoxamine engages multiple pathways to decrease memory loss in the APP/PS1 model of amyloid accumulation. Neurosci Lett. 2015;584:362-7 pubmed publisher
  • western blot; mouse
Biswas C, Shah N, Muthu M, La P, Fernando A, Sengupta S, et al. Nuclear heme oxygenase-1 (HO-1) modulates subcellular distribution and activation of Nrf2, impacting metabolic and anti-oxidant defenses. J Biol Chem. 2014;289:26882-94 pubmed publisher
  • western blot; mouse
Wang H, Sun W, Ma J, Pan Y, Wang L, Zhang W. Polycystin-1 mediates mechanical strain-induced osteoblastic mechanoresponses via potentiation of intracellular calcium and Akt/?-catenin pathway. PLoS ONE. 2014;9:e91730 pubmed publisher
Gao F, Shi L, Hines T, Hebbar S, Neufeld K, Smith D. Insulin signaling regulates a functional interaction between adenomatous polyposis coli and cytoplasmic dynein. Mol Biol Cell. 2017;28:587-599 pubmed publisher
Ma J, Wang Z, Liu C, Shen H, Chen Z, Yin J, et al. Pramipexole-Induced Hypothermia Reduces Early Brain Injury via PI3K/AKT/GSK3? pathway in Subarachnoid Hemorrhage rats. Sci Rep. 2016;6:23817 pubmed publisher
Otsuka R, Harada N, Aoki S, Shirai K, Nishitsuji K, Nozaki A, et al. C-terminal region of GADD34 regulates eIF2α dephosphorylation and cell proliferation in CHO-K1 cells. Cell Stress Chaperones. 2016;21:29-40 pubmed publisher
Schulz E, Klampfl P, Holzapfel S, Janecke A, Ulz P, Renner W, et al. Germline variants in the SEMA4A gene predispose to familial colorectal cancer type X. Nat Commun. 2014;5:5191 pubmed publisher
Fu X, Creighton C, Biswal N, Kumar V, Shea M, Herrera S, et al. Overcoming endocrine resistance due to reduced PTEN levels in estrogen receptor-positive breast cancer by co-targeting mammalian target of rapamycin, protein kinase B, or mitogen-activated protein kinase kinase. Breast Cancer Res. 2014;16:430 pubmed publisher
Thevenard J, Verzeaux L, Devy J, Etique N, Jeanne A, Schneider C, et al. Low-density lipoprotein receptor-related protein-1 mediates endocytic clearance of tissue inhibitor of metalloproteinases-1 and promotes its cytokine-like activities. PLoS ONE. 2014;9:e103839 pubmed publisher
Mirzaa G, Parry D, Fry A, Giamanco K, Schwartzentruber J, Vanstone M, et al. De novo CCND2 mutations leading to stabilization of cyclin D2 cause megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome. Nat Genet. 2014;46:510-515 pubmed publisher
Eigler T, Ben Shlomo A, Zhou C, Khalafi R, Ren S, Melmed S. Constitutive somatostatin receptor subtype-3 signaling suppresses growth hormone synthesis. Mol Endocrinol. 2014;28:554-64 pubmed publisher
Wang H, Geng Q, Wang L, Lu Y. Curcumin potentiates antitumor activity of L-asparaginase via inhibition of the AKT signaling pathway in acute lymphoblastic leukemia. Leuk Lymphoma. 2012;53:1376-82 pubmed publisher
product information
SKU :
9832S
Product-Name :
GSK-3β (3D10) Mouse mAb
Size :
100 ul
Price-(USD) :
235 USD
Species-x-Reactivity :
H, M, R, Hm, Mk
Applications :
Flow cytometry
Product-Category :
PI3K / Akt Signaling
Shipping-Temp :
AMBIENT
Storage-Temp :
-20°C
Product-Type :
Monoclonal Antibody
MW :
46
Host :
Mouse
Target :
GSK3-beta
Primary-Protein :
GSK3B
Alt-Names :
GSK-3 beta,GSK3B,GSK3beta isoform,Glycogen synthase kinase-3 beta,glycogen synthase kinase 3 beta
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
https://www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.