product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
Phospho-eIF4E (Ser209) Antibody
catalog :
9741
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
antigen modification :
phosphorylated
reactivity :
Aplysia, human, mouse, rat, pig
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 25
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; 1:1000; loading ...; fig s3d
Zhu P, Khatiwada S, Cui Y, Reineke L, Dooling S, Kim J, et al. Activation of the ISR mediates the behavioral and neurophysiological abnormalities in Down syndrome. Science. 2019;366:843-849 pubmed publisher
  • western blot; human; loading ...; fig 6c
Urtishak K, Wang L, Culjkovic Kraljacic B, Davenport J, Porazzi P, Vincent T, et al. Targeting EIF4E signaling with ribavirin in infant acute lymphoblastic leukemia. Oncogene. 2019;38:2241-2262 pubmed publisher
  • western blot; human; fig s5a
Chung H, Calis J, Wu X, Sun T, Yu Y, Sarbanes S, et al. Human ADAR1 Prevents Endogenous RNA from Triggering Translational Shutdown. Cell. 2018;172:811-824.e14 pubmed publisher
  • western blot; human; loading ...; fig s5p
Schafer S, Viswanathan S, Widjaja A, Lim W, Moreno Moral A, Delaughter D, et al. IL-11 is a crucial determinant of cardiovascular fibrosis. Nature. 2017;552:110-115 pubmed publisher
  • western blot; human; loading ...; fig 1e
Zhan Y, Guo J, Yang W, Goncalves C, Rzymski T, Dreas A, et al. MNK1/2 inhibition limits oncogenicity and metastasis of KIT-mutant melanoma. J Clin Invest. 2017;127:4179-4192 pubmed publisher
  • western blot; mouse; loading ...; fig 5b
Hsu J, Hubbell Engler B, Adelmant G, Huang J, Joyce C, Vazquez F, et al. PRMT1-Mediated Translation Regulation Is a Crucial Vulnerability of Cancer. Cancer Res. 2017;77:4613-4625 pubmed publisher
  • western blot; rat; loading ...; fig 8a
Ayuso M, Martínez Alonso E, Regidor I, Alcazar A. Stress Granule Induction after Brain Ischemia Is Independent of Eukaryotic Translation Initiation Factor (eIF) 2? Phosphorylation and Is Correlated with a Decrease in eIF4B and eIF4E Proteins. J Biol Chem. 2016;291:27252-27264 pubmed publisher
  • western blot; human; 1:1000; fig st1
Treindl F, Ruprecht B, Beiter Y, Schultz S, Döttinger A, Staebler A, et al. A bead-based western for high-throughput cellular signal transduction analyses. Nat Commun. 2016;7:12852 pubmed publisher
  • western blot; human; fig 4
Lyabin D, Ovchinnikov L. Selective regulation of YB-1 mRNA translation by the mTOR signaling pathway is not mediated by 4E-binding protein. Sci Rep. 2016;6:22502 pubmed publisher
  • western blot; human; fig 2
Su X, Yu Y, Zhong Y, Giannopoulou E, Hu X, Liu H, et al. Interferon-γ regulates cellular metabolism and mRNA translation to potentiate macrophage activation. Nat Immunol. 2015;16:838-849 pubmed publisher
  • western blot; human; 1:500; fig 2.e
Laberge R, Sun Y, Orjalo A, Patil C, Freund A, Zhou L, et al. MTOR regulates the pro-tumorigenic senescence-associated secretory phenotype by promoting IL1A translation. Nat Cell Biol. 2015;17:1049-61 pubmed publisher
  • western blot; human; fig 4
Wang J, Ma L, Tang X, Zhang X, Qiao Y, Shi Y, et al. Doxorubicin induces apoptosis by targeting Madcam1 and AKT and inhibiting protein translation initiation in hepatocellular carcinoma cells. Oncotarget. 2015;6:24075-91 pubmed
  • immunohistochemistry; human; loading ...; fig 2c
  • western blot; mouse; loading ...; fig 1c
Zidek L, Ackermann T, Hartleben G, Eichwald S, Kortman G, Kiehntopf M, et al. Deficiency in mTORC1-controlled C/EBPβ-mRNA translation improves metabolic health in mice. EMBO Rep. 2015;16:1022-36 pubmed publisher
  • immunoprecipitation; human; fig 4
  • immunocytochemistry; human; fig s5
  • western blot; human; 1:1000; fig 7
  • western blot; mouse; 1:1000; fig 3
Martínez A, Sesé M, Losa J, Robichaud N, Sonenberg N, Aasen T, et al. Phosphorylation of eIF4E Confers Resistance to Cellular Stress and DNA-Damaging Agents through an Interaction with 4E-T: A Rationale for Novel Therapeutic Approaches. PLoS ONE. 2015;10:e0123352 pubmed publisher
  • immunocytochemistry; Aplysia; fig 5
Kim S, Martin K. Neuron-wide RNA transport combines with netrin-mediated local translation to spatially regulate the synaptic proteome. elife. 2015;4: pubmed publisher
  • immunohistochemistry - paraffin section; human; fig 7
  • western blot; human; fig 4
Chen Y, Wei M, Wang C, Lee H, Pan S, Gao M, et al. Dual phosphoinositide 3-kinase/mammalian target of rapamycin inhibitor is an effective radiosensitizer for colorectal cancer. Cancer Lett. 2015;357:582-90 pubmed publisher
  • western blot; pig; 1:500
Zhang S, Ren M, Zeng X, He P, Ma X, Qiao S. Leucine stimulates ASCT2 amino acid transporter expression in porcine jejunal epithelial cell line (IPEC-J2) through PI3K/Akt/mTOR and ERK signaling pathways. Amino Acids. 2014;46:2633-42 pubmed publisher
  • western blot; mouse; 1:1000
Sidhu H, Dansie L, Hickmott P, Ethell D, Ethell I. Genetic removal of matrix metalloproteinase 9 rescues the symptoms of fragile X syndrome in a mouse model. J Neurosci. 2014;34:9867-79 pubmed publisher
  • western blot; mouse
Bian Z, Liao H, Zhang Y, Wu Q, Zhou H, Yang Z, et al. Never in mitosis gene A related kinase-6 attenuates pressure overload-induced activation of the protein kinase B pathway and cardiac hypertrophy. PLoS ONE. 2014;9:e96095 pubmed publisher
Park E, Lee Y, Oh T, Kim B, Lim B, Lim J. Vanillin Suppresses Cell Motility by Inhibiting STAT3-Mediated HIF-1? mRNA Expression in Malignant Melanoma Cells. Int J Mol Sci. 2017;18: pubmed publisher
Figueiredo V, Roberts L, Markworth J, Barnett M, Coombes J, Raastad T, et al. Impact of resistance exercise on ribosome biogenesis is acutely regulated by post-exercise recovery strategies. Physiol Rep. 2016;4: pubmed publisher
Sharma A, Yilmaz A, Marsh K, Cochrane A, Boris Lawrie K. Thriving under stress: selective translation of HIV-1 structural protein mRNA during Vpr-mediated impairment of eIF4E translation activity. PLoS Pathog. 2012;8:e1002612 pubmed publisher
Wu M, Liu H, Fannin J, Katta A, Wang Y, Arvapalli R, et al. Acetaminophen improves protein translational signaling in aged skeletal muscle. Rejuvenation Res. 2010;13:571-9 pubmed publisher
RamachandraRao S, Zhu Y, Ravasi T, McGowan T, Toh I, Dunn S, et al. Pirfenidone is renoprotective in diabetic kidney disease. J Am Soc Nephrol. 2009;20:1765-75 pubmed publisher
Ma D, Yu H, Lin D, Sun Y, Liu L, Liu Y, et al. S6K1 is involved in polyploidization through its phosphorylation at Thr421/Ser424. J Cell Physiol. 2009;219:31-44 pubmed publisher
product information
SKU :
9741S
Product-Name :
Phospho-eIF4E (Ser209) Antibody
Size :
100 ul
Price-(USD) :
274 USD
Species-x-Reactivity :
H, M, R, Mk
Applications :
Western blot
Product-Category :
Translational Control
Shipping-Temp :
AMBIENT
Storage-Temp :
-20°C
Product-Type :
Polyclonal Antibody
MW :
25
Host :
Rabbit
Target :
eIF4E (Ser209) phosphate
Primary-Protein :
eIF4E
Alt-Names :
CBP,EIF4E,EIF4E1,EIF4EL1,EIF4F,Eukaryotic translation initiation factor 4E,IF4E,MGC111573,eIF-4E,eIF-4F 25 kDa subunit,eukaryotic translation initiation factor 4E-like 1,mRNA cap-binding protein
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
https://www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.