product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
Di-Methyl-Histone H3 (Lys27) (D18C8) XP® Rabbit mAb
catalog :
9728
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
D18C8
reactivity :
human, mouse, rat
application :
western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, immunohistochemistry - free floating section, ChIP-Seq, other
more info or order :
Cell Signaling Technology product webpage
citations: 20
Published Application/Species/Sample/DilutionReference
  • western blot; human; 1:1000; loading ...; fig s3d
Piunti A, Smith E, Morgan M, Ugarenko M, Khaltyan N, Helmin K, et al. CATACOMB: An endogenous inducible gene that antagonizes H3K27 methylation activity of Polycomb repressive complex 2 via an H3K27M-like mechanism. Sci Adv. 2019;5:eaax2887 pubmed publisher
  • ChIP-Seq; mouse; loading ...; fig 5a
  • western blot; mouse; 1:1000; loading ...; fig 1b
Lavarone E, Barbieri C, Pasini D. Dissecting the role of H3K27 acetylation and methylation in PRC2 mediated control of cellular identity. Nat Commun. 2019;10:1679 pubmed publisher
  • western blot; mouse; 1:2000; loading ...; fig s6c
Li G, Ji T, Chen J, Fu Y, Hou L, Feng Y, et al. CRL4DCAF8 Ubiquitin Ligase Targets Histone H3K79 and Promotes H3K9 Methylation in the Liver. Cell Rep. 2017;18:1499-1511 pubmed publisher
  • other; human; 1:900; loading ...; fig 3
He Y, Selvaraju S, Curtin M, Jakob C, Zhu H, Comess K, et al. The EED protein-protein interaction inhibitor A-395 inactivates the PRC2 complex. Nat Chem Biol. 2017;13:389-395 pubmed publisher
  • ChIP-Seq; mouse; 1:40; loading ...; fig 2i
Ueda T, Nakata Y, Nagamachi A, Yamasaki N, Kanai A, Sera Y, et al. Propagation of trimethylated H3K27 regulated by polycomb protein EED is required for embryogenesis, hematopoietic maintenance, and tumor suppression. Proc Natl Acad Sci U S A. 2016;113:10370-5 pubmed publisher
  • western blot; human; fig s1
Kawano S, Grassian A, Tsuda M, Knutson S, Warholic N, Kuznetsov G, et al. Preclinical Evidence of Anti-Tumor Activity Induced by EZH2 Inhibition in Human Models of Synovial Sarcoma. PLoS ONE. 2016;11:e0158888 pubmed publisher
  • western blot; mouse; 1:1000; fig s13
Fei Q, Yang X, Jiang H, Wang Q, Yu Y, Yu Y, et al. SETDB1 modulates PRC2 activity at developmental genes independently of H3K9 trimethylation in mouse ES cells. Genome Res. 2015;25:1325-35 pubmed publisher
  • immunocytochemistry; mouse; 1:100
Huang X, Shen M, Wang L, Yu F, Wu W, Liu H. Effects of tributyltin chloride on developing mouse oocytes and preimplantation embryos. Microsc Microanal. 2015;21:358-67 pubmed publisher
  • western blot; human
Poirier J, Gardner E, Connis N, Moreira A, de Stanchina E, Hann C, et al. DNA methylation in small cell lung cancer defines distinct disease subtypes and correlates with high expression of EZH2. Oncogene. 2015;34:5869-78 pubmed publisher
  • immunocytochemistry; human
Luense S, Denner P, Fernández Montalván A, Hartung I, Husemann M, Stresemann C, et al. Quantification of histone H3 Lys27 trimethylation (H3K27me3) by high-throughput microscopy enables cellular large-scale screening for small-molecule EZH2 inhibitors. J Biomol Screen. 2015;20:190-201 pubmed publisher
  • western blot; human; 1:1000; fig 1
Hashizume R, Andor N, Ihara Y, Lerner R, Gan H, Chen X, et al. Pharmacologic inhibition of histone demethylation as a therapy for pediatric brainstem glioma. Nat Med. 2014;20:1394-6 pubmed publisher
  • immunohistochemistry - free floating section; mouse; 1:200
  • western blot; mouse; 1:1000
Subbanna S, Nagre N, Shivakumar M, Umapathy N, Psychoyos D, Basavarajappa B. Ethanol induced acetylation of histone at G9a exon1 and G9a-mediated histone H3 dimethylation leads to neurodegeneration in neonatal mice. Neuroscience. 2014;258:422-32 pubmed publisher
  • immunohistochemistry - free floating section; mouse; 1:200
  • western blot; mouse; 1:1000; fig 5a
Subbanna S, Shivakumar M, Umapathy N, Saito M, Mohan P, Kumar A, et al. G9a-mediated histone methylation regulates ethanol-induced neurodegeneration in the neonatal mouse brain. Neurobiol Dis. 2013;54:475-85 pubmed publisher
Lee C, Yu J, Kumar S, Jin Y, LeRoy G, Bhanu N, et al. Allosteric Activation Dictates PRC2 Activity Independent of Its Recruitment to Chromatin. Mol Cell. 2018;70:422-434.e6 pubmed publisher
Lee C, Holder M, Grau D, Saldaña Meyer R, Yu J, Ganai R, et al. Distinct Stimulatory Mechanisms Regulate the Catalytic Activity of Polycomb Repressive Complex 2. Mol Cell. 2018;70:435-448.e5 pubmed publisher
Conway E, Jerman E, Healy E, Ito S, Holoch D, Oliviero G, et al. A Family of Vertebrate-Specific Polycombs Encoded by the LCOR/LCORL Genes Balance PRC2 Subtype Activities. Mol Cell. 2018;70:408-421.e8 pubmed publisher
Huggins I, Bos T, Gaylord O, Jessen C, Lonquich B, Puranen A, et al. The WNT target SP5 negatively regulates WNT transcriptional programs in human pluripotent stem cells. Nat Commun. 2017;8:1034 pubmed publisher
Baker T, Nerle S, Pritchard J, Zhao B, Rivera V, Garner A, et al. Acquisition of a single EZH2 D1 domain mutation confers acquired resistance to EZH2-targeted inhibitors. Oncotarget. 2015;6:32646-55 pubmed publisher
Barsotti A, Ryskin M, Zhong W, Zhang W, Giannakou A, Loreth C, et al. Epigenetic reprogramming by tumor-derived EZH2 gain-of-function mutations promotes aggressive 3D cell morphologies and enhances melanoma tumor growth. Oncotarget. 2015;6:2928-38 pubmed
Schmitz S, Albert M, Malatesta M, Morey L, Johansen J, Bak M, et al. Jarid1b targets genes regulating development and is involved in neural differentiation. EMBO J. 2011;30:4586-600 pubmed publisher
product information
SKU :
9728P
Product-Name :
Di-Methyl-Histone H3 (Lys27) (D18C8) XP® Rabbit mAb
Size :
40 ul
Price-(USD) :
135 USD
Species-x-Reactivity :
H, M, R, Mk
Applications :
Chromatin Immunoprecipitation
Product-Category :
Chromatin Regulation / Nuclear Function
Shipping-Temp :
AMBIENT
Storage-Temp :
-20°C
Product-Type :
Monoclonal Antibody
MW :
17
Host :
Rabbit
Target :
H3 (Lys28) dimethylate
Primary-Protein :
H3
Alt-Names :
H3 histone family, member A,H3/A,H31,H3FA,H3FB,H3FC,H3FD,H3FF,H3FH,H3FI,H3FJ,H3FK,H3FL,HIST1H3A,HIST1H3B,HIST1H3C,HIST1H3D,HIST1H3E,HIST1H3F,HIST1H3G,HIST1H3H,HIST1H3I,HIST1H3J,Histone H3.1,Histone H3/a,Histone H3/b,Histone H3/c,Histone H3/d,Histone H3/f,Histone H3/h,Histone H3/i,Histone H3/j,Histone H3/k,Histone H3/l,histone 1, H3a,histone cluster 1, H3a
more info or order :
Cell Signaling Technology product webpage
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
https://www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.