product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
Tri-Methyl-Histone H3 (Lys4) Antibody
catalog :
9727
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat, chicken, pig
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, ChIP-Seq
more info or order :
Cell Signaling Technology product webpage
citations: 18
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; 1:2000; loading ...; fig s16c
Chakraborty A, Laukka T, Myllykoski M, Ringel A, Booker M, Tolstorukov M, et al. Histone demethylase KDM6A directly senses oxygen to control chromatin and cell fate. Science. 2019;363:1217-1222 pubmed publisher
  • chromatin immunoprecipitation; human; fig 4h
Chen Z, Gao Y, Yao L, Liu Y, Huang L, Yan Z, et al. LncFZD6 initiates Wnt/β-catenin and liver TIC self-renewal through BRG1-mediated FZD6 transcriptional activation. Oncogene. 2018;37:3098-3112 pubmed publisher
  • western blot; human; 1:2000; loading ...; fig 2a
Chen S, Jing Y, Kang X, Yang L, Wang D, Zhang W, et al. Histone H2B monoubiquitination is a critical epigenetic switch for the regulation of autophagy. Nucleic Acids Res. 2017;45:1144-1158 pubmed publisher
  • ChIP-Seq; chicken; fig 3
Romanello M, Schiavone D, Frey A, Sale J. Histone H3.3 promotes IgV gene diversification by enhancing formation of AID-accessible single-stranded DNA. EMBO J. 2016;35:1452-64 pubmed publisher
  • chromatin immunoprecipitation; human; loading ...; fig s9c
Cui Q, Yang S, Ye P, Tian E, Sun G, Zhou J, et al. Downregulation of TLX induces TET3 expression and inhibits glioblastoma stem cell self-renewal and tumorigenesis. Nat Commun. 2016;7:10637 pubmed publisher
  • chromatin immunoprecipitation; mouse; loading ...; fig 4c
Hessmann E, Zhang J, Chen N, Hasselluhn M, Liou G, Storz P, et al. NFATc4 Regulates Sox9 Gene Expression in Acinar Cell Plasticity and Pancreatic Cancer Initiation. Stem Cells Int. 2016;2016:5272498 pubmed publisher
  • western blot; human
Fan H, Zhang H, Pascuzzi P, Andrisani O. Hepatitis B virus X protein induces EpCAM expression via active DNA demethylation directed by RelA in complex with EZH2 and TET2. Oncogene. 2016;35:715-26 pubmed publisher
  • western blot; human; 1:2000; fig 1b
Yu J, Ramasamy T, Murphy N, Holt M, Czapiewski R, Wei S, et al. PI3K/mTORC2 regulates TGF-β/Activin signalling by modulating Smad2/3 activity via linker phosphorylation. Nat Commun. 2015;6:7212 pubmed publisher
  • western blot; mouse; 1:10,000; fig 1
Lu W, Liu S, Li B, Xie Y, Adhiambo C, Yang Q, et al. SKP2 inactivation suppresses prostate tumorigenesis by mediating JARID1B ubiquitination. Oncotarget. 2015;6:771-88 pubmed
  • western blot; pig
Banduseela V, Chen Y, Kultima H, Norman H, Aare S, Radell P, et al. Impaired autophagy, chaperone expression, and protein synthesis in response to critical illness interventions in porcine skeletal muscle. Physiol Genomics. 2013;45:477-86 pubmed publisher
  • immunocytochemistry; mouse; 1:250
Ma P, Pan H, Montgomery R, Olson E, Schultz R. Compensatory functions of histone deacetylase 1 (HDAC1) and HDAC2 regulate transcription and apoptosis during mouse oocyte development. Proc Natl Acad Sci U S A. 2012;109:E481-9 pubmed publisher
Mendoza Parra M, Saravaki V, Cholley P, Blum M, Billoré B, Gronemeyer H. Antibody performance in ChIP-sequencing assays: From quality scores of public data sets to quantitative certification. F1000Res. 2016;5:54 pubmed publisher
Schiavone D, Jozwiakowski S, Romanello M, Guilbaud G, Guilliam T, Bailey L, et al. PrimPol Is Required for Replicative Tolerance of G Quadruplexes in Vertebrate Cells. Mol Cell. 2016;61:161-9 pubmed publisher
Papadopoulou C, Guilbaud G, Schiavone D, Sale J. Nucleotide Pool Depletion Induces G-Quadruplex-Dependent Perturbation of Gene Expression. Cell Rep. 2015;13:2491-2503 pubmed publisher
Kang N, Chen P, Chen Y, Zeng H, He X, Zhu Y. PRMT6 mediates CSE induced inflammation and apoptosis. Int Immunopharmacol. 2015;24:95-101 pubmed publisher
Schiavone D, Guilbaud G, Murat P, Papadopoulou C, Sarkies P, Prioleau M, et al. Determinants of G quadruplex-induced epigenetic instability in REV1-deficient cells. EMBO J. 2014;33:2507-20 pubmed publisher
Eram M, Bustos S, Lima Fernandes E, Siarheyeva A, Senisterra G, Hajian T, et al. Trimethylation of histone H3 lysine 36 by human methyltransferase PRDM9 protein. J Biol Chem. 2014;289:12177-88 pubmed publisher
Song N, Liu J, An S, Nishino T, Hishikawa Y, Koji T. Immunohistochemical Analysis of Histone H3 Modifications in Germ Cells during Mouse Spermatogenesis. Acta Histochem Cytochem. 2011;44:183-90 pubmed publisher
product information
SKU :
9727S
Product-Name :
Tri-Methyl-Histone H3 (Lys4) Antibody
Size :
100 ul
Price-(USD) :
274 USD
Species-x-Reactivity :
H, M, R, Mk, (X, Z)
Applications :
Chromatin Immunoprecipitation
Product-Category :
Chromatin Regulation / Nuclear Function
Shipping-Temp :
COLD
Storage-Temp :
-20°C
Product-Type :
Polyclonal Antibody
MW :
17
Host :
Rabbit
Target :
H3 (Lys5) trimethylate
Primary-Protein :
H3
Alt-Names :
H3 histone family, member A,H3/A,H31,H3FA,H3FB,H3FC,H3FD,H3FF,H3FH,H3FI,H3FJ,H3FK,H3FL,HIST1H3A,HIST1H3B,HIST1H3C,HIST1H3D,HIST1H3E,HIST1H3F,HIST1H3G,HIST1H3H,HIST1H3I,HIST1H3J,Histone H3.1,Histone H3/a,Histone H3/b,Histone H3/c,Histone H3/d,Histone H3/f,Histone H3/h,Histone H3/i,Histone H3/j,Histone H3/k,Histone H3/l,histone 1, H3a,histone cluster 1, H3a
more info or order :
Cell Signaling Technology product webpage
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
https://www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.