product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb (Alexa Fluor® 488 Conjugate)
catalog :
9719
clonality :
monoclonal
host :
domestic rabbit
conjugate :
AF488
antigen modification :
phosphorylated
clone name :
20E3
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry
citations: 27
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig 2c
Jia Y, Kang X, Tan L, Ren Y, Qu L, Tang J, et al. Nicotinamide Mononucleotide Attenuates Renal Interstitial Fibrosis After AKI by Suppressing Tubular DNA Damage and Senescence. Front Physiol. 2021;12:649547 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig e2i
Wilkinson A, Ishida R, Kikuchi M, Sudo K, Morita M, Crisostomo R, et al. Long-term ex vivo haematopoietic-stem-cell expansion allows nonconditioned transplantation. Nature. 2019;: pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 5c
  • western blot; mouse; loading ...; fig 5a
Hou Y, Lautrup S, Cordonnier S, Wang Y, Croteau D, Zavala E, et al. NAD+ supplementation normalizes key Alzheimer's features and DNA damage responses in a new AD mouse model with introduced DNA repair deficiency. Proc Natl Acad Sci U S A. 2018;115:E1876-E1885 pubmed publisher
  • flow cytometry; human; 1:40; loading ...; fig 6C
  • flow cytometry; mouse; 1:50; fig 6C
Kostrzak A, Caval V, Escande M, Pliquet E, Thalmensi J, Bestetti T, et al. APOBEC3A intratumoral DNA electroporation in mice. Gene Ther. 2017;24:74-83 pubmed publisher
  • flow cytometry; human; fig 5
Rebolleda N, Losada Fernandez I, Perez Chacon G, Castejon R, Rosado S, Morado M, et al. Synergistic Activity of Deguelin and Fludarabine in Cells from Chronic Lymphocytic Leukemia Patients and in the New Zealand Black Murine Model. PLoS ONE. 2016;11:e0154159 pubmed publisher
  • immunocytochemistry; human; 1:500; loading ...; fig s1d
Del Bel Belluz L, Guidi R, Pateras I, Levi L, Mihaljevic B, Rouf S, et al. The Typhoid Toxin Promotes Host Survival and the Establishment of a Persistent Asymptomatic Infection. PLoS Pathog. 2016;12:e1005528 pubmed publisher
  • immunohistochemistry; human; 1:250; fig 4
Jun S, Jung Y, Suh H, Wang W, Kim M, Oh Y, et al. LIG4 mediates Wnt signalling-induced radioresistance. Nat Commun. 2016;7:10994 pubmed publisher
  • immunocytochemistry; mouse; 1:50; fig 1
  • immunohistochemistry; mouse; 1:50; fig 1
  • western blot; mouse; 1:5000; fig 1
Liu X, Chandramouly G, Rass E, Guan Y, Wang G, Hobbs R, et al. LRF maintains genome integrity by regulating the non-homologous end joining pathway of DNA repair. Nat Commun. 2015;6:8325 pubmed publisher
  • western blot; mouse; 1:1000; fig 4
Jang C, Shibata Y, Starmer J, Yee D, Magnuson T. Histone H3.3 maintains genome integrity during mammalian development. Genes Dev. 2015;29:1377-92 pubmed publisher
  • immunohistochemistry; mouse; fig 1
Sykora P, Misiak M, Wang Y, Ghosh S, Leandro G, Liu D, et al. DNA polymerase β deficiency leads to neurodegeneration and exacerbates Alzheimer disease phenotypes. Nucleic Acids Res. 2015;43:943-59 pubmed publisher
  • immunocytochemistry; mouse; 1:100; fig 2
Tanabe M, Tamura H, Taketani T, Okada M, Lee L, Tamura I, et al. Melatonin protects the integrity of granulosa cells by reducing oxidative stress in nuclei, mitochondria, and plasma membranes in mice. J Reprod Dev. 2015;61:35-41 pubmed publisher
  • flow cytometry; human; 1:50; loading ...; fig 3i
Greve K, Lindgreen J, Terp M, Pedersen C, Schmidt S, Mollenhauer J, et al. Ectopic expression of cancer/testis antigen SSX2 induces DNA damage and promotes genomic instability. Mol Oncol. 2015;9:437-49 pubmed publisher
Oben K, Gachuki B, Alhakeem S, McKenna M, Liang Y, St Clair D, et al. Radiation Induced Apoptosis of Murine Bone Marrow Cells Is Independent of Early Growth Response 1 (EGR1). PLoS ONE. 2017;12:e0169767 pubmed publisher
Cheung J, Dickinson D, Moss J, Schuler M, Spellman R, Heard P. Histone markers identify the mode of action for compounds positive in the TK6 micronucleus assay. Mutat Res Genet Toxicol Environ Mutagen. 2015;777:7-16 pubmed publisher
Kilic Eren M, Tabor V. The role of hypoxia inducible factor-1 alpha in bypassing oncogene-induced senescence. PLoS ONE. 2014;9:e101064 pubmed publisher
Bais M, Ozdener G, Sonenshein G, Trackman P. Effects of tumor-suppressor lysyl oxidase propeptide on prostate cancer xenograft growth and its direct interactions with DNA repair pathways. Oncogene. 2015;34:1928-37 pubmed publisher
Hall M, Telma K, Chang K, Lee T, Madigan J, Lloyd J, et al. Say no to DMSO: dimethylsulfoxide inactivates cisplatin, carboplatin, and other platinum complexes. Cancer Res. 2014;74:3913-22 pubmed publisher
Kubota S, Fukumoto Y, Ishibashi K, Soeda S, Kubota S, Yuki R, et al. Activation of the prereplication complex is blocked by mimosine through reactive oxygen species-activated ataxia telangiectasia mutated (ATM) protein without DNA damage. J Biol Chem. 2014;289:5730-46 pubmed publisher
Nasuno T, Mimaki S, Okamoto M, Esumi H, Tsuchihara K. Effect of a poly(ADP-ribose) polymerase-1 inhibitor against esophageal squamous cell carcinoma cell lines. Cancer Sci. 2014;105:202-10 pubmed publisher
Bakkenist C, Czambel R, Clump D, Greenberger J, Beumer J, Schmitz J. Radiation therapy induces the DNA damage response in peripheral blood. Oncotarget. 2013;4:1143-8 pubmed
Sun B, Ross S, Trask O, Carmichael P, Dent M, White A, et al. Assessing dose-dependent differences in DNA-damage, p53 response and genotoxicity for quercetin and curcumin. Toxicol In Vitro. 2013;27:1877-87 pubmed publisher
Volotskova O, Hawley T, Stepp M, Keidar M. Targeting the cancer cell cycle by cold atmospheric plasma. Sci Rep. 2012;2:636 pubmed publisher
Hu C, Zhang S, Gao X, Gao X, Xu X, Lv Y, et al. Roles of Kruppel-associated Box (KRAB)-associated Co-repressor KAP1 Ser-473 Phosphorylation in DNA Damage Response. J Biol Chem. 2012;287:18937-52 pubmed publisher
Wei S, Xiong M, Zhan D, Liang B, Wang Y, Gutmann D, et al. Ku80 functions as a tumor suppressor in hepatocellular carcinoma by inducing S-phase arrest through a p53-dependent pathway. Carcinogenesis. 2012;33:538-47 pubmed publisher
Pei X, Dai Y, Youssefian L, Chen S, Bodie W, Takabatake Y, et al. Cytokinetically quiescent (G0/G1) human multiple myeloma cells are susceptible to simultaneous inhibition of Chk1 and MEK1/2. Blood. 2011;118:5189-200 pubmed publisher
Negrotto S, Hu Z, Alcazar O, Ng K, Triozzi P, Lindner D, et al. Noncytotoxic differentiation treatment of renal cell cancer. Cancer Res. 2011;71:1431-41 pubmed publisher
Gagou M, Zuazua Villar P, Meuth M. Enhanced H2AX phosphorylation, DNA replication fork arrest, and cell death in the absence of Chk1. Mol Biol Cell. 2010;21:739-52 pubmed publisher
product information
SKU :
9719S
Product-Name :
Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb (Alexa Fluor® 488 Conjugate)
Size :
100 ul
Price-(USD) :
291 USD
Species-x-Reactivity :
H, M
Applications :
Flow cytometry
Product-Category :
Chromatin Regulation / Nuclear Function
Shipping-Temp :
COLD
Storage-Temp :
4°C
Product-Type :
Monoclonal Antibody
Host :
Rabbit
Target :
H2AX (Ser140) phosphate
Primary-Protein :
H2AX
Alt-Names :
H2A histone family, member X,H2A.X,H2AFX,H2AX,H2AX histone,H2a/x,Histone H2A.x
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
https://www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.