product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
Phospho-Histone H3 (Ser10) (6G3) Mouse mAb
catalog :
9706
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
antigen modification :
phosphorylated
clone name :
6G3
reactivity :
human, mouse, rat, dogs, chicken, Drosophila melanogaster, Xenopus laevis
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section
citations: 51
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:25; loading ...; fig s4b
Rhodes J, Feldmann A, Hernández Rodríguez B, Díaz N, Brown J, Fursova N, et al. Cohesin Disrupts Polycomb-Dependent Chromosome Interactions in Embryonic Stem Cells. Cell Rep. 2020;30:820-835.e10 pubmed publisher
  • immunohistochemistry; Drosophila melanogaster; 1:1000; loading ...; fig 2s2e
Abdusselamoglu M, Eroglu E, Burkard T, Knoblich J. The transcription factor odd-paired regulates temporal identity in transit-amplifying neural progenitors via an incoherent feed-forward loop. elife. 2019;8: pubmed publisher
  • immunohistochemistry; mouse; 1:200; loading ...; fig 2b
Roy A, Murphy R, Deng M, MacDonald J, Bammler T, Aldinger K, et al. PI3K-Yap activity drives cortical gyrification and hydrocephalus in mice. elife. 2019;8: pubmed publisher
  • western blot; human; fig S1A
Wyatt H, Laister R, Martin S, Arrowsmith C, West S. The SMX DNA Repair Tri-nuclease. Mol Cell. 2017;65:848-860.e11 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 2b
Zaqout S, Bessa P, Kramer N, Stoltenburg Didinger G, Kaindl A. CDK5RAP2 Is Required to Maintain the Germ Cell Pool during Embryonic Development. Stem Cell Reports. 2017;8:198-204 pubmed publisher
  • immunocytochemistry; human; fig 1a
Yamauchi M, Shibata A, Suzuki K, Suzuki M, Niimi A, Kondo H, et al. Regulation of pairing between broken DNA-containing chromatin regions by Ku80, DNA-PKcs, ATM, and 53BP1. Sci Rep. 2017;7:41812 pubmed publisher
  • immunocytochemistry; human; loading ...; fig s1
Vakana E, Pratt S, Blosser W, Dowless M, Simpson N, Yuan X, et al. LY3009120, a panRAF inhibitor, has significant anti-tumor activity in BRAF and KRAS mutant preclinical models of colorectal cancer. Oncotarget. 2017;8:9251-9266 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:100; fig 4
Wefers A, Lindner S, Schulte J, Schüller U. Overexpression of Lin28b in Neural Stem Cells is Insufficient for Brain Tumor Formation, but Induces Pathological Lobulation of the Developing Cerebellum. Cerebellum. 2017;16:122-131 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:300; loading ...; fig 4a
Bosch P, Fuller L, Sleeth C, Weiner J. Akirin2 is essential for the formation of the cerebral cortex. Neural Dev. 2016;11:21 pubmed
  • flow cytometry; human; loading ...; fig 2a
Bridges K, Chen X, Liu H, Rock C, Buchholz T, Shumway S, et al. MK-8776, a novel chk1 kinase inhibitor, radiosensitizes p53-defective human tumor cells. Oncotarget. 2016;7:71660-71672 pubmed publisher
  • flow cytometry; rat; fig s2
Bott L, Salomons F, Maric D, Liu Y, Merry D, Fischbeck K, et al. The polyglutamine-expanded androgen receptor responsible for spinal and bulbar muscular atrophy inhibits the APC/C(Cdh1) ubiquitin ligase complex. Sci Rep. 2016;6:27703 pubmed publisher
  • immunohistochemistry; rat; 1:500; fig 4
Kirita Y, Kami D, Ishida R, Adachi T, Tamagaki K, Matoba S, et al. Preserved Nephrogenesis Following Partial Nephrectomy in Early Neonates. Sci Rep. 2016;6:26792 pubmed publisher
  • flow cytometry; mouse; 1:50; fig 3
Brosh R, Hrynyk I, Shen J, Waghray A, Zheng N, Lemischka I. A dual molecular analogue tuner for dissecting protein function in mammalian cells. Nat Commun. 2016;7:11742 pubmed publisher
  • western blot; human; loading ...; fig 3f
Matsushima H, Mori T, Ito F, Yamamoto T, Akiyama M, Kokabu T, et al. Anti-tumor effect of estrogen-related receptor alpha knockdown on uterine endometrial cancer. Oncotarget. 2016;7:34131-48 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:100; fig 6
Jardé T, Kass L, Staples M, Lescesen H, Carne P, Oliva K, et al. ERBB3 Positively Correlates with Intestinal Stem Cell Markers but Marks a Distinct Non Proliferative Cell Population in Colorectal Cancer. PLoS ONE. 2015;10:e0138336 pubmed publisher
  • western blot; dogs; 1:1000; fig 3b
Tuncay H, Brinkmann B, Steinbacher T, Schürmann A, Gerke V, Iden S, et al. JAM-A regulates cortical dynein localization through Cdc42 to control planar spindle orientation during mitosis. Nat Commun. 2015;6:8128 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:1000
Shimada M, Dumitrache L, Russell H, McKinnon P. Polynucleotide kinase-phosphatase enables neurogenesis via multiple DNA repair pathways to maintain genome stability. EMBO J. 2015;34:2465-80 pubmed publisher
  • immunocytochemistry; human; 1:1000; fig 1
Kanfer G, Courtheoux T, Peterka M, Meier S, Soste M, Melnik A, et al. Mitotic redistribution of the mitochondrial network by Miro and Cenp-F. Nat Commun. 2015;6:8015 pubmed publisher
  • immunocytochemistry; mouse; 1:1000; fig 2
Guo Y, Zheng Y. Lamins position the nuclear pores and centrosomes by modulating dynein. Mol Biol Cell. 2015;26:3379-89 pubmed publisher
  • immunocytochemistry; human; 1:400; fig 2a
Massey A. Multiparametric Cell Cycle Analysis Using the Operetta High-Content Imager and Harmony Software with PhenoLOGIC. PLoS ONE. 2015;10:e0134306 pubmed publisher
  • western blot; human; 1:1000
Ohira M, Iwasaki Y, Tanaka C, Kuroki M, Matsuo N, Kitamura T, et al. A novel anti-microtubule agent with carbazole and benzohydrazide structures suppresses tumor cell growth in vivo. Biochim Biophys Acta. 2015;1850:1676-84 pubmed publisher
  • flow cytometry; human
  • immunocytochemistry; human
Hasegawa H, Ishibashi K, Kubota S, Yamaguchi C, Yuki R, Nakajo H, et al. Cdk1-mediated phosphorylation of human ATF7 at Thr-51 and Thr-53 promotes cell-cycle progression into M phase. PLoS ONE. 2014;9:e116048 pubmed publisher
  • immunohistochemistry; mouse; fig 4
Hill R, Kuijper S, Lindsey J, Petrie K, Schwalbe E, Barker K, et al. Combined MYC and P53 defects emerge at medulloblastoma relapse and define rapidly progressive, therapeutically targetable disease. Cancer Cell. 2015;27:72-84 pubmed publisher
  • immunohistochemistry - frozen section; Xenopus laevis; 1:3000
  • western blot; chicken
  • western blot; human
Eifler M, Uecker R, Weisbach H, Bogdanow B, Richter E, König L, et al. PUL21a-Cyclin A2 interaction is required to protect human cytomegalovirus-infected cells from the deleterious consequences of mitotic entry. PLoS Pathog. 2014;10:e1004514 pubmed publisher
  • immunocytochemistry; human
Kim K, Ossipova O, Sokol S. Neural crest specification by inhibition of the ROCK/Myosin II pathway. Stem Cells. 2015;33:674-85 pubmed publisher
  • western blot; human; 1:1000
Muenyi C, Trivedi A, Helm C, States J. Cisplatin plus sodium arsenite and hyperthermia induces pseudo-G1 associated apoptotic cell death in ovarian cancer cells. Toxicol Sci. 2014;139:74-82 pubmed publisher
  • immunocytochemistry; mouse; 1:200; fig 4
Schröder Heurich B, Wieland B, Lavin M, Schindler D, Dork T. Protective role of RAD50 on chromatin bridges during abnormal cytokinesis. FASEB J. 2014;28:1331-41 pubmed publisher
  • immunocytochemistry; mouse
  • western blot; mouse
Luebben S, Kawabata T, Akre M, Lee W, Johnson C, O Sullivan M, et al. Helq acts in parallel to Fancc to suppress replication-associated genome instability. Nucleic Acids Res. 2013;41:10283-97 pubmed publisher
  • immunocytochemistry; human; 1:500
  • western blot; human; 1:1000
Tan E, Caro S, Potnis A, Lanza C, Slawson C. O-linked N-acetylglucosamine cycling regulates mitotic spindle organization. J Biol Chem. 2013;288:27085-99 pubmed publisher
  • immunohistochemistry - free floating section; mouse; 1:100
Tong K, Kwan K. Common partner Smad-independent canonical bone morphogenetic protein signaling in the specification process of the anterior rhombic lip during cerebellum development. Mol Cell Biol. 2013;33:1925-37 pubmed publisher
  • immunocytochemistry; human
Li L, Yang G, Ren C, Tanimoto R, Hirayama T, Wang J, et al. Glioma pathogenesis-related protein 1 induces prostate cancer cell death through Hsc70-mediated suppression of AURKA and TPX2. Mol Oncol. 2013;7:484-96 pubmed publisher
  • western blot; mouse
Qi W, Spier C, Liu X, Agarwal A, Cooke L, Persky D, et al. Alisertib (MLN8237) an investigational agent suppresses Aurora A and B activity, inhibits proliferation, promotes endo-reduplication and induces apoptosis in T-NHL cell lines supporting its importance in PTCL treatment. Leuk Res. 2013;37:434-9 pubmed publisher
  • flow cytometry; human
  • immunocytochemistry; human
Blakemore L, Boes C, Cordell R, Manson M. Curcumin-induced mitotic arrest is characterized by spindle abnormalities, defects in chromosomal congression and DNA damage. Carcinogenesis. 2013;34:351-60 pubmed publisher
  • immunohistochemistry; mouse; 1:100
Farioli Vecchioli S, Micheli L, Saraulli D, Ceccarelli M, Cannas S, Scardigli R, et al. Btg1 is Required to Maintain the Pool of Stem and Progenitor Cells of the Dentate Gyrus and Subventricular Zone. Front Neurosci. 2012;6:124 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:200
Wu F, Sapkota D, Li R, Mu X. Onecut 1 and Onecut 2 are potential regulators of mouse retinal development. J Comp Neurol. 2012;520:952-69 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:100
Eckler M, McKenna W, Taghvaei S, McConnell S, Chen B. Fezf1 and Fezf2 are required for olfactory development and sensory neuron identity. J Comp Neurol. 2011;519:1829-46 pubmed publisher
  • immunohistochemistry; mouse; 1:200
Wang B, Lufkin T, Rubenstein J. Dlx6 regulates molecular properties of the striatum and central nucleus of the amygdala. J Comp Neurol. 2011;519:2320-34 pubmed publisher
  • immunohistochemistry - free floating section; mouse; 1:100; fig 3
Farioli Vecchioli S, Saraulli D, Costanzi M, Leonardi L, Cinà I, Micheli L, et al. Impaired terminal differentiation of hippocampal granule neurons and defective contextual memory in PC3/Tis21 knockout mice. PLoS ONE. 2009;4:e8339 pubmed publisher
Mimura Y, Takagi M, Clever M, Imamoto N. ELYS regulates the localization of LBR by modulating its phosphorylation state. J Cell Sci. 2016;129:4200-4212 pubmed
Fedoseienko A, Wieringa H, Wisman G, Duiker E, Reyners A, Hofker M, et al. Nuclear COMMD1 Is Associated with Cisplatin Sensitivity in Ovarian Cancer. PLoS ONE. 2016;11:e0165385 pubmed publisher
Anderson M, Schimmang T, Lewandoski M. An FGF3-BMP Signaling Axis Regulates Caudal Neural Tube Closure, Neural Crest Specification and Anterior-Posterior Axis Extension. PLoS Genet. 2016;12:e1006018 pubmed publisher
Seidel H, Kimble J. Cell-cycle quiescence maintains Caenorhabditis elegans germline stem cells independent of GLP-1/Notch. elife. 2015;4: pubmed publisher
Luebben S, Kawabata T, Johnson C, O Sullivan M, Shima N. A concomitant loss of dormant origins and FANCC exacerbates genome instability by impairing DNA replication fork progression. Nucleic Acids Res. 2014;42:5605-15 pubmed publisher
Hell M, Duda M, Weber T, Moch H, Krek W. Tumor suppressor VHL functions in the control of mitotic fidelity. Cancer Res. 2014;74:2422-31 pubmed publisher
Gallego Paez L, Tanaka H, Bando M, Takahashi M, Nozaki N, Nakato R, et al. Smc5/6-mediated regulation of replication progression contributes to chromosome assembly during mitosis in human cells. Mol Biol Cell. 2014;25:302-17 pubmed publisher
Lancaster M, Renner M, Martin C, Wenzel D, Bicknell L, Hurles M, et al. Cerebral organoids model human brain development and microcephaly. Nature. 2013;501:373-9 pubmed publisher
Gavvovidis I, Rost I, Trimborn M, Kaiser F, Purps J, Wiek C, et al. A novel MCPH1 isoform complements the defective chromosome condensation of human MCPH1-deficient cells. PLoS ONE. 2012;7:e40387 pubmed publisher
Matthews H, Delabre U, Rohn J, Guck J, Kunda P, Baum B. Changes in Ect2 localization couple actomyosin-dependent cell shape changes to mitotic progression. Dev Cell. 2012;23:371-83 pubmed publisher
Shin J, Padmanabhan A, de Groh E, Lee J, Haidar S, Dahlberg S, et al. Zebrafish neurofibromatosis type 1 genes have redundant functions in tumorigenesis and embryonic development. Dis Model Mech. 2012;5:881-94 pubmed publisher
Ribas V, Gonçalves B, Linden R, Chiarini L. Activation of c-Jun N-terminal kinase (JNK) during mitosis in retinal progenitor cells. PLoS ONE. 2012;7:e34483 pubmed publisher
McGuinness B, Hirota T, Kudo N, Peters J, Nasmyth K. Shugoshin prevents dissociation of cohesin from centromeres during mitosis in vertebrate cells. PLoS Biol. 2005;3:e86 pubmed
product information
SKU :
9706S
Product-Name :
Phospho-Histone H3 (Ser10) (6G3) Mouse mAb
Size :
100 ul
Price-(USD) :
274 USD
Species-x-Reactivity :
H, M, R
Applications :
Flow cytometry
Product-Category :
Chromatin Regulation / Nuclear Function
Shipping-Temp :
AMBIENT
Storage-Temp :
-20°C
Product-Type :
Monoclonal Antibody
MW :
17
Host :
Mouse
Target :
H3 (Ser11) phosphate
Primary-Protein :
H3
Alt-Names :
H3 histone family, member A,H3/A,H31,H3FA,H3FB,H3FC,H3FD,H3FF,H3FH,H3FI,H3FJ,H3FK,H3FL,HIST1H3A,HIST1H3B,HIST1H3C,HIST1H3D,HIST1H3E,HIST1H3F,HIST1H3G,HIST1H3H,HIST1H3I,HIST1H3J,Histone H3.1,Histone H3/a,Histone H3/b,Histone H3/c,Histone H3/d,Histone H3/f,Histone H3/h,Histone H3/i,Histone H3/j,Histone H3/k,Histone H3/l,histone 1, H3a,histone cluster 1, H3a
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
https://www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.