Smad3 (C67H9) Rabbit mAb | Cell Signaling Technology 9523 product information

product summary

company name :

Cell Signaling Technology

product type :

antibody

product name :

Smad3 (C67H9) Rabbit mAb

catalog :

9523

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

C67H9

reactivity :

human, mouse, rat, bovine

application :

western blot, immunocytochemistry, immunoprecipitation, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section, proximity ligation assay, western blot knockout validation, immunocytochemistry knockout validation

more info or order :

Cell Signaling Technology product webpage

citations: 122

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry knockout validation; human; loading ...; fig 1d western blot knockout validation; human; 1:1000; loading ...; fig 1b	Bertrand Chapel A, Caligaris C, Fenouil T, Savary C, Aires S, Martel S, et al. SMAD2/3 mediate oncogenic effects of TGF-β in the absence of SMAD4. Commun Biol. 2022;5:1068 pubmed publisher
immunocytochemistry; human; 1:200; fig 4g	Cheng P, Wirka R, Kim J, Kim H, Nguyen T, Kundu R, et al. Smad3 regulates smooth muscle cell fate and mediates adverse remodeling and calcification of the atherosclerotic plaque. Nat Cardiovasc Res. 2022;1:322-333 pubmed publisher
western blot; human; 1:1000; loading ...; fig 2c	Wei T, Richter G, Zhang H, Sun R, Smith C, STRUB G. Extracranial arteriovenous malformations demonstrate dysregulated TGF-β/BMP signaling and increased circulating TGF-β1. Sci Rep. 2022;12:16612 pubmed publisher
western blot; human; loading ...; fig 5f	Ni H, Zhang H, Li L, Huang H, Guo H, Zhang L, et al. T cell-intrinsic STING signaling promotes regulatory T cell induction and immunosuppression by upregulating FOXP3 transcription in cervical cancer. J Immunother Cancer. 2022;10: pubmed publisher
western blot; human; 1:1000; loading ...; fig 3k	Zhang T, Xia W, Song X, Mao Q, Huang X, Chen B, et al. Super-enhancer hijacking LINC01977 promotes malignancy of early-stage lung adenocarcinoma addicted to the canonical TGF-β/SMAD3 pathway. J Hematol Oncol. 2022;15:114 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 1k	Chakrabarti M, Bhattacharya A, Gebere M, Johnson J, Ayub Z, Chatzistamou I, et al. Increased TGFβ1 and SMAD3 Contribute to Age-Related Aortic Valve Calcification. Front Cardiovasc Med. 2022;9:770065 pubmed publisher
western blot; human; 1:1000; loading ...; fig 1b western blot; mouse; 1:1000; loading ...; fig s1a	Forouhan M, Lim W, Zanetti Domingues L, Tynan C, Roberts T, Malik B, et al. AR cooperates with SMAD4 to maintain skeletal muscle homeostasis. Acta Neuropathol. 2022;143:713-731 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 7d	Amirrad F, Pala R, Shamloo K, Muntean B, Nauli S. Arrhythmogenic Hearts in PKD2 Mutant Mice Are Characterized by Cardiac Fibrosis, Systolic, and Diastolic Dysfunctions. Front Cardiovasc Med. 2021;8:772961 pubmed publisher
western blot; human; loading ...; fig 6a	Jacquet M, Hervouet E, Baudu T, Herfs M, Parratte C, Feugeas J, et al. GABARAPL1 Inhibits EMT Signaling through SMAD-Tageted Negative Feedback. Biology (Basel). 2021;10: pubmed publisher
western blot; human; fig 5f western blot; rat; fig 3b	Shi Y, Hu Y, Wang Y, Ma X, Tang L, Tao M, et al. Blockade of Autophagy Prevents the Development and Progression of Peritoneal Fibrosis. Front Pharmacol. 2021;12:724141 pubmed publisher
chromatin immunoprecipitation; mouse; loading ...; fig 6a western blot; mouse; 1:1000; loading ...; fig 2d	Savorani C, Malinverno M, Seccia R, Maderna C, Giannotta M, Terreran L, et al. A dual role of YAP in driving TGFβ-mediated endothelial-to-mesenchymal transition. J Cell Sci. 2021;134: pubmed publisher
western blot; mouse; 1:1000; loading ...; fig s9d	Matsuzawa T, Morita M, Shimane A, Otsuka R, Mei Y, Irie F, et al. Heparan sulfate promotes differentiation of white adipocytes to maintain insulin sensitivity and glucose homeostasis. J Biol Chem. 2021;:101006 pubmed publisher
western blot; bovine; 1:1000; loading ...; fig 3a	Chen F, Sheng L, Xu C, Li J, Ali I, Li H, et al. Ufbp1, a Key Player of Ufm1 Conjugation System, Protects Against Ketosis-Induced Liver Injury via Suppressing Smad3 Activation. Front Cell Dev Biol. 2021;9:676789 pubmed publisher
western blot; human; 1:1000; fig 5e	Bruce J, To K, Lui V, Chung G, Chan Y, Tsang C, et al. Whole-genome profiling of nasopharyngeal carcinoma reveals viral-host co-operation in inflammatory NF-κB activation and immune escape. Nat Commun. 2021;12:4193 pubmed publisher
western blot; mouse; 1:250; loading ...; fig 7c	Barbé C, Loumaye A, Lause P, Ritvos O, Thissen J. p21-Activated Kinase 1 Is Permissive for the Skeletal Muscle Hypertrophy Induced by Myostatin Inhibition. Front Physiol. 2021;12:677746 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 14g	Hsieh Y, Lee K, Lei H, Lan K, Huo T, Lin Y, et al. (Pro)renin Receptor Knockdown Attenuates Liver Fibrosis Through Inactivation of ERK/TGF-β1/SMAD3 Pathway. Cell Mol Gastroenterol Hepatol. 2021;12:813-838 pubmed publisher
western blot; human; 1:1000; loading ...; fig 5b	Niu B, Liu J, Lv B, Lin J, Li X, Wu C, et al. Interplay between transforming growth factor-β and Nur77 in dual regulations of inhibitor of differentiation 1 for colonic tumorigenesis. Nat Commun. 2021;12:2809 pubmed publisher
western blot; human; fig s9c	Fang W, Sofia Acevedo D, Smart C, Zinda B, Alissa N, Warren K, et al. Expression of CCL2/CCR2 signaling proteins in breast carcinoma cells is associated with invasive progression. Sci Rep. 2021;11:8708 pubmed publisher
western blot; rat; 1:1000; fig 6e	Meng K, Cai H, Cai S, Hong Y, Zhang X. Adiponectin Modified BMSCs Alleviate Heart Fibrosis via Inhibition TGF-beta1/Smad in Diabetic Rats. Front Cell Dev Biol. 2021;9:644160 pubmed publisher
western blot; human; 1:100; loading ...; fig 4a	Liu X, Zhao X, Duan X, Wang X, Wang T, Feng S, et al. Knockout of NGAL aggravates tubulointerstitial injury in a mouse model of diabetic nephropathy by enhancing oxidative stress and fibrosis. Exp Ther Med. 2021;21:321 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig s4a	Chen Y, Jhao P, Hung C, Wu Y, Lin S, Chiang W, et al. Endoplasmic reticulum protein TXNDC5 promotes renal fibrosis by enforcing TGF-β signaling in kidney fibroblasts. J Clin Invest. 2021;131: pubmed publisher
western blot; human; loading ...; fig 5a	Nam B, Park H, Lee Y, Oh Y, Park J, Kim S, et al. TGFβ1 Suppressed Matrix Mineralization of Osteoblasts Differentiation by Regulating SMURF1-C/EBPβ-DKK1 Axis. Int J Mol Sci. 2020;21: pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 6c	Sun Z, Ji N, Ma Q, Zhu R, Chen Z, Wang Z, et al. Epithelial-Mesenchymal Transition in Asthma Airway Remodeling Is Regulated by the IL-33/CD146 Axis. Front Immunol. 2020;11:1598 pubmed publisher
western blot; human; loading ...; fig s2	Shoemaker L, McCormick A, Allen B, Chang S. Evidence for endothelial-to-mesenchymal transition in human brain arteriovenous malformations. Clin Transl Med. 2020;10:e99 pubmed publisher
western blot; mouse; loading ...; fig 6b	Chakrabarti M, Al Sammarraie N, Gebere M, Bhattacharya A, Chopra S, Johnson J, et al. Transforming Growth Factor Beta3 is Required for Cardiovascular Development. J Cardiovasc Dev Dis. 2020;7: pubmed publisher
western blot; rat; 1:1000; loading ...; fig 5c	Yang L, Han B, Zhang M, Wang Y, Tao K, Zhu M, et al. Activation of BK Channels Prevents Hepatic Stellate Cell Activation and Liver Fibrosis Through the Suppression of TGFβ1/SMAD3 and JAK/STAT3 Profibrotic Signaling Pathways. Front Pharmacol. 2020;11:165 pubmed publisher
western blot; human; 1:100; loading ...; fig 5c	Wang X, Lu Q, Fei X, Zhao Y, Shi B, Li C, et al. Expression and Prognostic Value of Id-4 in Patients with Esophageal Squamous Cell Carcinoma. Onco Targets Ther. 2020;13:1225-1234 pubmed publisher
western blot; human; 1:1000; loading ...; fig 6a	Ye D, Zhu J, Zhao Q, Ma W, Xiao Y, Xu G, et al. LMP1 Up-regulates Calreticulin to Induce Epithelial-mesenchymal Transition via TGF-β/Smad3/NRP1 Pathway in Nasopharyngeal Carcinoma Cells. J Cancer. 2020;11:1257-1269 pubmed publisher
western blot; human; 1:1000; loading ...; fig 3c	Cheng X, Haeberle S, Luca Shytaj I, Gama Brambila R, Theobald J, Ghafoory S, et al. NHC-gold compounds mediate immune suppression through induction of AHR-TGFβ1 signalling in vitro and in scurfy mice. Commun Biol. 2020;3:10 pubmed publisher
western blot; mouse; loading ...; fig s9d	Lin F, Meng X, Guo Y, Cao W, Liu W, Xia Q, et al. Epigenetic initiation of the TH17 differentiation program is promoted by Cxxc finger protein 1. Sci Adv. 2019;5:eaax1608 pubmed publisher
western blot; human; 1:1000; loading ...; fig 4g	Gal H, Lysenko M, Stroganov S, Vadai E, Youssef S, Tzadikevitch Geffen K, et al. Molecular pathways of senescence regulate placental structure and function. EMBO J. 2019;38:e100849 pubmed publisher
western blot; human; 1:750; loading ...; fig 5e	Minuesa G, Albanese S, Xie W, Kazansky Y, Worroll D, Chow A, et al. Small-molecule targeting of MUSASHI RNA-binding activity in acute myeloid leukemia. Nat Commun. 2019;10:2691 pubmed publisher
western blot; human; 1:1000; loading ...; fig 3b	Tsai C, Tsai C, Yi J, Kao H, Huang Y, Wang C, et al. Activin A regulates the epidermal growth factor receptor promoter by activating the PI3K/SP1 pathway in oral squamous cell carcinoma cells. Sci Rep. 2019;9:5197 pubmed publisher
western blot; mouse; loading ...; fig 4c	Li J, Wang Y, Ma M, Jiang S, Zhang X, Zhang Y, et al. Autocrine CTHRC1 activates hepatic stellate cells and promotes liver fibrosis by activating TGF-β signaling. EBioMedicine. 2019;40:43-55 pubmed publisher
western blot; mouse; loading ...; fig 4b	MacFarlane E, Parker S, Shin J, Kang B, Ziegler S, Creamer T, et al. Lineage-specific events underlie aortic root aneurysm pathogenesis in Loeys-Dietz syndrome. J Clin Invest. 2019;129:659-675 pubmed publisher
western blot; human; 1:1000; loading ...; fig 5a	Peng J, Liang S, Li L. sFRP1 exerts effects on gastric cancer cells through GSK3β/Rac1‑mediated restraint of TGFβ/Smad3 signaling. Oncol Rep. 2019;41:224-234 pubmed publisher
western blot; mouse; 1:500; loading ...; fig s2	Lam J, van den Bosch M, Wegrzyn J, Parker J, Ibrahim R, Slowski K, et al. miR-143/145 differentially regulate hematopoietic stem and progenitor activity through suppression of canonical TGFβ signaling. Nat Commun. 2018;9:2418 pubmed publisher
western blot; human; loading ...; fig 4a	Zhao H, Klausen C, Li Y, Zhu H, Wang Y, Leung P. Bone morphogenetic protein 2 promotes human trophoblast cell invasion by upregulating N-cadherin via non-canonical SMAD2/3 signaling. Cell Death Dis. 2018;9:174 pubmed publisher
western blot; rat; 1:1000; loading ...; fig 5b	Zhang R, Wu Y, Xie F, Zhong Y, Wang Y, Xu M, et al. RGMa mediates reactive astrogliosis and glial scar formation through TGF?1/Smad2/3 signaling after stroke. Cell Death Differ. 2018;25:1503-1516 pubmed publisher
western blot; human; loading ...; fig s6c	Schwab A, Siddiqui A, Vazakidou M, Napoli F, Böttcher M, Menchicchi B, et al. Polyol Pathway Links Glucose Metabolism to the Aggressiveness of Cancer Cells. Cancer Res. 2018;78:1604-1618 pubmed publisher
western blot; human; 1:1000; fig 2c	Bajikar S, Wang C, Borten M, Pereira E, Atkins K, Janes K. Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor Sequestration in Triple-Negative Breast Cancer. Dev Cell. 2017;43:418-435.e13 pubmed publisher
chromatin immunoprecipitation; human; loading ...; fig 3d western blot; human; loading ...; fig 2f	Dufton N, Peghaire C, Osuna Almagro L, Raimondi C, Kalna V, Chuahan A, et al. Dynamic regulation of canonical TGFÎ² signalling by endothelial transcription factor ERG protects from liver fibrogenesis. Nat Commun. 2017;8:895 pubmed publisher
western blot; mouse; loading ...; fig 5c	Fu G, Xu Q, Qiu Y, Jin X, Xu T, Dong S, et al. Suppression of Th17 cell differentiation by misshapen/NIK-related kinase MINK1. J Exp Med. 2017;214:1453-1469 pubmed publisher
western blot; mouse; loading ...; fig 1c	Hammers D, Merscham Banda M, Hsiao J, ENGST S, Hartman J, Sweeney H. Supraphysiological levels of GDF11 induce striated muscle atrophy. EMBO Mol Med. 2017;9:531-544 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 8b	Chatterjee A, Kosmacek E, Oberley Deegan R. MnTE-2-PyP Treatment, or NOX4 Inhibition, Protects against Radiation-Induced Damage in Mouse Primary Prostate Fibroblasts by Inhibiting the TGF-Beta 1 Signaling Pathway. Radiat Res. 2017;187:367-381 pubmed publisher
western blot; mouse; 1:1000; fig 5b	Xu Z, Greenblatt M, Yan G, Feng H, Sun J, Lotinun S, et al. SMURF2 regulates bone homeostasis by disrupting SMAD3 interaction with vitamin D receptor in osteoblasts. Nat Commun. 2017;8:14570 pubmed publisher
western blot; human; loading ...; fig 7c	He S, Cao Y, Xie P, Dong G, Zhang L. The Nedd8 Non-covalent Binding Region in the Smurf HECT Domain is Critical to its Ubiquitn Ligase Function. Sci Rep. 2017;7:41364 pubmed publisher
western blot; human; 1:1000; loading ...; fig 6b	Gopal S, Veracini L, Grall D, Butori C, Schaub S, Audebert S, et al. Fibronectin-guided migration of carcinoma collectives. Nat Commun. 2017;8:14105 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 8a	Choi S, Piao Z, Jin L, Kim J, Kim G, Ryu Y, et al. Piceatannol Attenuates Renal Fibrosis Induced by Unilateral Ureteral Obstruction via Downregulation of Histone Deacetylase 4/5 or p38-MAPK Signaling. PLoS ONE. 2016;11:e0167340 pubmed publisher
western blot; human; loading ...; fig 2c	Bryson B, Junk D, Cipriano R, Jackson M. STAT3-mediated SMAD3 activation underlies Oncostatin M-induced Senescence. Cell Cycle. 2017;16:319-334 pubmed publisher
western blot; human; 1:1000; loading ...; fig 7d	Zhu L, Gómez Durán A, Saretzki G, Jin S, Tilgner K, Melguizo Sanchís D, et al. The mitochondrial protein CHCHD2 primes the differentiation potential of human induced pluripotent stem cells to neuroectodermal lineages. J Cell Biol. 2016;215:187-202 pubmed
chromatin immunoprecipitation; mouse; loading ...; fig 5b	Wang W, Song B, Anbarchian T, Shirazyan A, Sadik J, Lyons K. Smad2 and Smad3 Regulate Chondrocyte Proliferation and Differentiation in the Growth Plate. PLoS Genet. 2016;12:e1006352 pubmed publisher
western blot; mouse; loading ...; fig s2a	Yang P, Schmit B, Fu C, Desart K, Oh S, Berceli S, et al. Smooth muscle cell-specific Tgfbr1 deficiency promotes aortic aneurysm formation by stimulating multiple signaling events. Sci Rep. 2016;6:35444 pubmed publisher
western blot; human; 1:1000; loading ...; fig 3d	Queisser A, Hagedorn S, Wang H, Schaefer T, Konantz M, Alavi S, et al. Ecotropic viral integration site 1, a novel oncogene in prostate cancer. Oncogene. 2017;36:1573-1584 pubmed publisher
western blot; rat; fig 5	Hesler R, Huang J, Starr M, Treboschi V, Bernanke A, Nixon A, et al. TGF-?-induced stromal CYR61 promotes resistance to gemcitabine in pancreatic ductal adenocarcinoma through downregulation of the nucleoside transporters hENT1 and hCNT3. Carcinogenesis. 2016;37:1041-1051 pubmed publisher
western blot; human; 1:1000; fig s5	Cammareri P, Rose A, Vincent D, Wang J, Nagano A, Libertini S, et al. Inactivation of TGFβ receptors in stem cells drives cutaneous squamous cell carcinoma. Nat Commun. 2016;7:12493 pubmed publisher
western blot; human; 1:500; loading ...; fig s1b	Elsafadi M, Manikandan M, Dawud R, Alajez N, Hamam R, Alfayez M, et al. Transgelin is a TGFÎ²-inducible gene that regulates osteoblastic and adipogenic differentiation of human skeletal stem cells through actin cytoskeleston organization. Cell Death Dis. 2016;7:e2321 pubmed publisher
western blot; human; 1:1000; loading ...; fig 3d	Kim D, Chang M, Yoon C, Middeldorp J, Martinez O, Byeon S, et al. Epstein-Barr virus BARF1-induced NFÎºB/miR-146a/SMAD4 alterations in stomach cancer cells. Oncotarget. 2016;7:82213-82227 pubmed publisher
western blot; rat; loading ...; fig 3e	Kang L, Zhang D, Ma C, Zhang J, Jia K, Liu J, et al. Cinnamaldehyde and allopurinol reduce fructose-induced cardiac inflammation and fibrosis by attenuating CD36-mediated TLR4/6-IRAK4/1 signaling to suppress NLRP3 inflammasome activation. Sci Rep. 2016;6:27460 pubmed publisher
western blot; human; loading ...; fig 4f	Oh T, Wang S, Acharya B, Goode J, Graham J, Clarke C, et al. The Nuclear Receptor, ROR?, Regulates Pathways Necessary for Breast Cancer Metastasis. EBioMedicine. 2016;6:59-72 pubmed publisher
western blot; human; loading ...; fig 3a	Kurimoto R, Iwasawa S, Ebata T, Ishiwata T, Sekine I, Tada Y, et al. Drug resistance originating from a TGF-β/FGF-2-driven epithelial-to-mesenchymal transition and its reversion in human lung adenocarcinoma cell lines harboring an EGFR mutation. Int J Oncol. 2016;48:1825-36 pubmed publisher
immunocytochemistry; human; fig s10	Chang H, Liu Y, Xue M, Liu H, Du S, Zhang L, et al. Synergistic action of master transcription factors controls epithelial-to-mesenchymal transition. Nucleic Acids Res. 2016;44:2514-27 pubmed publisher
western blot; human; 1:1000; fig 1a	Yang W, Deng Y, Hsieh Y, Wu K, Kuo M. Thrombin Activates Latent TGF?1 via Integrin ?v?1 in Gingival Fibroblasts. J Dent Res. 2016;95:939-45 pubmed publisher
immunocytochemistry; human; fig 4 western blot; human; fig 4	Wang G, Yu Y, Sun C, Liu T, Liang T, Zhan L, et al. STAT3 selectively interacts with Smad3 to antagonize TGF-Î² signalling. Oncogene. 2016;35:4388-98 pubmed publisher
western blot; human; 1:1000; fig 5	Huan C, Yang T, Liang J, Xie T, Cheng L, Liu N, et al. Methylation-mediated BMPER expression in fibroblast activation in vitro and lung fibrosis in mice in vivo. Sci Rep. 2015;5:14910 pubmed publisher
western blot; mouse; 1:1000; fig 2	Xiao X, Shi X, Fan Y, Zhang X, Wu M, Lan P, et al. GITR subverts Foxp3(+) Tregs to boost Th9 immunity through regulation of histone acetylation. Nat Commun. 2015;6:8266 pubmed publisher
western blot; human; 1:1000; fig 7	Zhou X, Tao Y, Liang C, Zhang Y, Li H, Chen Q. BMP3 Alone and Together with TGF-Î² Promote the Differentiation of Human Mesenchymal Stem Cells into a Nucleus Pulposus-Like Phenotype. Int J Mol Sci. 2015;16:20344-59 pubmed publisher
western blot; mouse; fig 5	Lee S, Litan A, Li Z, Graves B, Lindsey S, Barwe S, et al. Na,K-ATPase Î²1-subunit is a target of sonic hedgehog signaling and enhances medulloblastoma tumorigenicity. Mol Cancer. 2015;14:159 pubmed publisher
western blot; human	Faura Tellez G, Vandepoele K, Brouwer U, Koning H, Elderman R, Hackett T, et al. Protocadherin-1 binds to SMAD3 and suppresses TGF-Î²1-induced gene transcription. Am J Physiol Lung Cell Mol Physiol. 2015;309:L725-35 pubmed publisher
proximity ligation assay; mouse; fig s12 chromatin immunoprecipitation; mouse; fig s15	Yoon J, Sudo K, Kuroda M, Kato M, Lee I, Han J, et al. Phosphorylation status determines the opposing functions of Smad2/Smad3 as STAT3 cofactors in TH17 differentiation. Nat Commun. 2015;6:7600 pubmed publisher
western blot; human; 1:1000; fig 5	Ghiabi P, Jiang J, Pasquier J, Maleki M, Abu Kaoud N, Halabi N, et al. Breast cancer cells promote a notch-dependent mesenchymal phenotype in endothelial cells participating to a pro-tumoral niche. J Transl Med. 2015;13:27 pubmed publisher
western blot; mouse; 1:1000; fig 7	Tontonoz P, Cortez Toledo O, Wroblewski K, Hong C, Lim L, Carranza R, et al. The orphan nuclear receptor Nur77 is a determinant of myofiber size and muscle mass in mice. Mol Cell Biol. 2015;35:1125-38 pubmed publisher
immunoprecipitation; mouse; loading ...; fig 7j western blot; mouse; loading ...; fig 7h western blot; human; loading ...; fig s3g	Li Q, Zou J, Wang M, Ding X, Chepelev I, Zhou X, et al. Critical role of histone demethylase Jmjd3 in the regulation of CD4+ T-cell differentiation. Nat Commun. 2014;5:5780 pubmed publisher
western blot; human; fig 5	Menon M, Chuang P, Li Z, Wei C, Zhang W, Luan Y, et al. Intronic locus determines SHROOM3 expression and potentiates renal allograft fibrosis. J Clin Invest. 2015;125:208-21 pubmed publisher
immunocytochemistry; human western blot; human	Zhang M, Zhang J, Liu S, Wang Q, Lin G, Qiu R, et al. NS5ATP9 suppresses activation of human hepatic stellate cells, possibly via inhibition of Smad3/phosphorylated-Smad3 expression. Inflammation. 2015;38:278-89 pubmed publisher
western blot; mouse; fig 6	Matsuyama M, Nomori A, Nakakuni K, Shimono A, Fukushima M. Secreted Frizzled-related protein 1 (Sfrp1) regulates the progression of renal fibrosis in a mouse model of obstructive nephropathy. J Biol Chem. 2014;289:31526-33 pubmed publisher
western blot; mouse; fig 2,3	Flanders K, Heger C, Conway C, Tang B, Sato M, Dengler S, et al. Brightfield proximity ligation assay reveals both canonical and mixed transforming growth factor-Î²/bone morphogenetic protein Smad signaling complexes in tissue sections. J Histochem Cytochem. 2014;62:846-63 pubmed publisher
western blot; mouse; 1:1000	Watatani H, Maeshima Y, Hinamoto N, Yamasaki H, Ujike H, Tanabe K, et al. Vasohibin-1 deficiency enhances renal fibrosis and inflammation after unilateral ureteral obstruction. Physiol Rep. 2014;2: pubmed publisher
western blot; rat	Yi X, Li X, Zhou Y, Ren S, Wan W, Feng G, et al. Hepatocyte growth factor regulates the TGF-?1-induced proliferation, differentiation and secretory function of cardiac fibroblasts. Int J Mol Med. 2014;34:381-90 pubmed publisher
chromatin immunoprecipitation; mouse; 1:50; fig 1d	Feuerborn A, Mathow D, Srivastava P, Gretz N, Grone H. Basonuclin-1 modulates epithelial plasticity and TGF-?1-induced loss of epithelial cell integrity. Oncogene. 2015;34:1185-95 pubmed publisher
western blot; mouse	Ikeda Y, Ozono I, Tajima S, Imao M, Horinouchi Y, Izawa Ishizawa Y, et al. Iron chelation by deferoxamine prevents renal interstitial fibrosis in mice with unilateral ureteral obstruction. PLoS ONE. 2014;9:e89355 pubmed publisher
western blot; rat	Beaudoin M, Snook L, Arkell A, Stefanson A, Wan Z, Simpson J, et al. Novel effects of rosiglitazone on SMAD2 and SMAD3 signaling in white adipose tissue of diabetic rats. Obesity (Silver Spring). 2014;22:1632-42 pubmed publisher
western blot; human; fig 1	Holtzhausen A, Golzio C, How T, Lee Y, Schiemann W, Katsanis N, et al. Novel bone morphogenetic protein signaling through Smad2 and Smad3 to regulate cancer progression and development. FASEB J. 2014;28:1248-67 pubmed publisher
western blot; mouse; 1:1000; fig 7	Das R, Xu S, Quan X, Nguyen T, Kong I, Chung C, et al. Upregulation of mitochondrial Nox4 mediates TGF-?-induced apoptosis in cultured mouse podocytes. Am J Physiol Renal Physiol. 2014;306:F155-67 pubmed publisher
western blot; human	Sun J, He H, Pillai S, Xiong Y, Challa S, Xu L, et al. GATA3 transcription factor abrogates Smad4 transcription factor-mediated fascin overexpression, invadopodium formation, and breast cancer cell invasion. J Biol Chem. 2013;288:36971-82 pubmed publisher
western blot; mouse	Maddaluno L, Rudini N, Cuttano R, Bravi L, Giampietro C, Corada M, et al. EndMT contributes to the onset and progression of cerebral cavernous malformations. Nature. 2013;498:492-6 pubmed publisher
western blot; mouse; fig 2 western blot; human	Sakaki Yumoto M, Liu J, Ramalho Santos M, Yoshida N, Derynck R. Smad2 is essential for maintenance of the human and mouse primed pluripotent stem cell state. J Biol Chem. 2013;288:18546-60 pubmed publisher
immunohistochemistry - paraffin section; human western blot; human; fig 4	Cigna N, Farrokhi Moshai E, Brayer S, Marchal Sommé J, Wemeau Stervinou L, Fabre A, et al. The hedgehog system machinery controls transforming growth factor-?-dependent myofibroblastic differentiation in humans: involvement in idiopathic pulmonary fibrosis. Am J Pathol. 2012;181:2126-37 pubmed publisher
	Yu Y, Liu X, Zhao Z, Xu Z, Qiao Y, Zhou Y, et al. The Extracellular Matrix Enriched With Exosomes for the Treatment on Pulmonary Fibrosis in Mice. Front Pharmacol. 2021;12:747223 pubmed publisher
	Huang Y, Cheng A, Tang H, Huang G, Cai L, Lin T, et al. USP7 facilitates SMAD3 autoregulation to repress cancer progression in p53-deficient lung cancer. Cell Death Dis. 2021;12:880 pubmed publisher
	Paw M, Wnuk D, Kądziołka D, Sęk A, Lasota S, Czyz J, et al. Fenofibrate Reduces the Asthma-Related Fibroblast-To-Myofibroblast Transition by TGF-Β/Smad2/3 Signaling Attenuation and Connexin 43-Dependent Phenotype Destabilization. Int J Mol Sci. 2018;19: pubmed publisher
	Kasetti R, Maddineni P, Patel P, Searby C, Sheffield V, Zode G. Transforming growth factor β2 (TGFβ2) signaling plays a key role in glucocorticoid-induced ocular hypertension. J Biol Chem. 2018;293:9854-9868 pubmed publisher
	Rai N, Veeroju S, Schymura Y, Janssen W, Wietelmann A, Kojonazarov B, et al. Effect of Riociguat and Sildenafil on Right Heart Remodeling and Function in Pressure Overload Induced Model of Pulmonary Arterial Banding. Biomed Res Int. 2018;2018:3293584 pubmed publisher
	Wang Y, Zhang X, Huang H, Xia Y, Yao Y, Mak A, et al. Osteocalcin expressing cells from tendon sheaths in mice contribute to tendon repair by activating Hedgehog signaling. elife. 2017;6: pubmed publisher
	Bernard K, Logsdon N, Benavides G, SANDERS Y, Zhang J, Darley Usmar V, et al. Glutaminolysis is required for transforming growth factor-β1-induced myofibroblast differentiation and activation. J Biol Chem. 2018;293:1218-1228 pubmed publisher
	Frick C, Yarka C, Nunns H, Goentoro L. Sensing relative signal in the Tgf-?/Smad pathway. Proc Natl Acad Sci U S A. 2017;114:E2975-E2982 pubmed publisher
	Junk D, Bryson B, Smigiel J, Parameswaran N, Bartel C, Jackson M. Oncostatin M promotes cancer cell plasticity through cooperative STAT3-SMAD3 signaling. Oncogene. 2017;36:4001-4013 pubmed publisher
	Zieba J, Zhang W, Chong J, Forlenza K, Martin J, Heard K, et al. A postnatal role for embryonic myosin revealed by MYH3 mutations that alter TGFβ signaling and cause autosomal dominant spondylocarpotarsal synostosis. Sci Rep. 2017;7:41803 pubmed publisher
	Han C, Koo J, Kim S, Gardenghi S, Rivella S, Strnad P, et al. Hepcidin inhibits Smad3 phosphorylation in hepatic stellate cells by impeding ferroportin-mediated regulation of Akt. Nat Commun. 2016;7:13817 pubmed publisher
	Chung H, Ryu D, Kim K, Chang J, Kim Y, Yi H, et al. Growth differentiation factor 15 is a myomitokine governing systemic energy homeostasis. J Cell Biol. 2017;216:149-165 pubmed publisher
	Zhao B, Guan H, Liu J, Zheng Z, Zhou Q, Zhang J, et al. Hypoxia drives the transition of human dermal fibroblasts to a myofibroblast-like phenotype via the TGF-?1/Smad3 pathway. Int J Mol Med. 2017;39:153-159 pubmed publisher
	Seo E, Kim W, Hur J, Kim H, Nam S, Choi A, et al. The Hippo-Salvador signaling pathway regulates renal tubulointerstitial fibrosis. Sci Rep. 2016;6:31931 pubmed publisher
	Liu Z, Ning G, Xu R, Cao Y, Meng A, Wang Q. Fscn1 is required for the trafficking of TGF-β family type I receptors during endoderm formation. Nat Commun. 2016;7:12603 pubmed publisher
	Sun S, Liu D, Deng Y, Zhang X, Wan D, Xi B, et al. SIX1 coordinates with TGF? signals to induce epithelial-mesenchymal transition in cervical cancer. Oncol Lett. 2016;12:1271-1278 pubmed
	Rodríguez Nieves J, Patalano S, Almanza D, Gharaee Kermani M, Macoska J. CXCL12/CXCR4 Axis Activation Mediates Prostate Myofibroblast Phenoconversion through Non-Canonical EGFR/MEK/ERK Signaling. PLoS ONE. 2016;11:e0159490 pubmed publisher
	Xiong S, Klausen C, Cheng J, Leung P. Activin B promotes endometrial cancer cell migration by down-regulating E-cadherin via SMAD-independent MEK-ERK1/2-SNAIL signaling. Oncotarget. 2016;7:40060-40072 pubmed publisher
	Chen J, Raju G, Jogunoori W, Menon V, Majumdar A, Chen J, et al. Mutational Profiles Reveal an Aberrant TGF-β-CEA Regulated Pathway in Colon Adenomas. PLoS ONE. 2016;11:e0153933 pubmed publisher
	Xiong S, Klausen C, Cheng J, Zhu H, Leung P. Activin B induces human endometrial cancer cell adhesion, migration and invasion by up-regulating integrin Î²3 via SMAD2/3 signaling. Oncotarget. 2015;6:31659-73 pubmed publisher
	Jeong M, Kim S, Kang H, Park K, Park W, Yang S, et al. Cucurbitacin I Attenuates Cardiomyocyte Hypertrophy via Inhibition of Connective Tissue Growth Factor (CCN2) and TGF- Î²/Smads Signalings. PLoS ONE. 2015;10:e0136236 pubmed publisher
	Fang W, Mafuvadze B, Yao M, Zou A, Portsche M, Cheng N. TGF-Î² Negatively Regulates CXCL1 Chemokine Expression in Mammary Fibroblasts through Enhancement of Smad2/3 and Suppression of HGF/c-Met Signaling Mechanisms. PLoS ONE. 2015;10:e0135063 pubmed publisher
	Thomas A, Eijgelaar W, Daemen M, Newby A. Foam Cell Formation In Vivo Converts Macrophages to a Pro-Fibrotic Phenotype. PLoS ONE. 2015;10:e0128163 pubmed publisher
	Yan B, Guo Q, Nan X, Wang Z, Yin Z, Yi L, et al. Micro-ribonucleic acid 29b inhibits cell proliferation and invasion and enhances cell apoptosis and chemotherapy effects of cisplatin via targeting of DNMT3b and AKT3 in prostate cancer. Onco Targets Ther. 2015;8:557-65 pubmed publisher
	Li Y, Klausen C, Cheng J, Zhu H, Leung P. Activin A, B, and AB increase human trophoblast cell invasion by up-regulating N-cadherin. J Clin Endocrinol Metab. 2014;99:E2216-25 pubmed publisher
	Kasai A, Ishimaru Y, Higashino K, Kobayashi K, Yamamuro A, Yoshioka Y, et al. Inhibition of apelin expression switches endothelial cells from proliferative to mature state in pathological retinal angiogenesis. Angiogenesis. 2013;16:723-34 pubmed publisher
	Han G, Bian L, Li F, Cotrim A, Wang D, Lu J, et al. Preventive and therapeutic effects of Smad7 on radiation-induced oral mucositis. Nat Med. 2013;19:421-8 pubmed publisher
	Guo Z, Khattar M, Schroder P, Miyahara Y, Wang G, He X, et al. A dynamic dual role of IL-2 signaling in the two-step differentiation process of adaptive regulatory T cells. J Immunol. 2013;190:3153-62 pubmed publisher
	Nakamura K, Nakano S, Miyoshi T, Yamanouchi K, Nishihara M. Loss of SPARC in mouse skeletal muscle causes myofiber atrophy. Muscle Nerve. 2013;48:791-9 pubmed publisher
	Sarközi R, Flucher K, Haller V, Pirklbauer M, Mayer G, Schramek H. Oncostatin M inhibits TGF-?1-induced CTGF expression via STAT3 in human proximal tubular cells. Biochem Biophys Res Commun. 2012;424:801-6 pubmed publisher
	Michael D, Salter R, Ramji D. TGF-? inhibits the uptake of modified low density lipoprotein by human macrophages through a Smad-dependent pathway: a dominant role for Smad-2. Biochim Biophys Acta. 2012;1822:1608-16 pubmed publisher
	Xu B, Chen H, Xu W, Zhang W, Buckley S, Zheng S, et al. Molecular mechanisms of MMP9 overexpression and its role in emphysema pathogenesis of Smad3-deficient mice. Am J Physiol Lung Cell Mol Physiol. 2012;303:L89-96 pubmed publisher
	Louafi F, Martinez Nunez R, Sanchez Elsner T. MicroRNA-155 targets SMAD2 and modulates the response of macrophages to transforming growth factor-{beta}. J Biol Chem. 2010;285:41328-36 pubmed publisher
	Li Q, Pangas S, Jorgez C, Graff J, Weinstein M, Matzuk M. Redundant roles of SMAD2 and SMAD3 in ovarian granulosa cells in vivo. Mol Cell Biol. 2008;28:7001-11 pubmed publisher

product information

SKU :

9523S

Product-Name :

Smad3 (C67H9) Rabbit mAb

Size :

100 ul

Price-(USD) :

235 USD

Species-x-Reactivity :

H, M, R, Mk, (X, Z, B)

Applications :

Chromatin Immunoprecipitation

Product-Category :

Developmental Biology

Shipping-Temp :

AMBIENT

Storage-Temp :

-20°C

Product-Type :

Monoclonal Antibody

MW :

Host :

Rabbit

Target :

Smad3

Primary-Protein :

Smad3

Alt-Names :

DKFZp586N0721,DKFZp686J10186,HSPC193,HsT17436,JV15-2,MAD homolog 3,MAD, mothers against decapentaplegic homolog 3,MADH3,MGC60396,Mad3,Mothers against DPP homolog 3,Mothers against decapentaplegic homolog 3,SMA- and MAD-related protein 3,SMAD 3,SMAD family member 3,SMAD, mothers against DPP homolog 3,SMAD3,hMAD-3,hSMAD3,mad homolog JV15-2,mad protein homolog

more info or order :

Cell Signaling Technology product webpage