| Published Application/Species/Dilution | Reference |
|---|
- western blot; human; 1:1000; fig 4
| Xu X, Tan X, Hulshoff M, Wilhelmi T, Zeisberg M, Zeisberg E. Hypoxia-induced endothelial-mesenchymal transition is associated with RASAL1 promoter hypermethylation in human coronary endothelial cells. FEBS Lett. 2016;590:1222-33 pubmed publisher |
- western blot; mouse; fig 2
| Hwang S, Jang S, Kim M, Kim L, Kim B, Kim H, et al. YY1 inhibits differentiation and function of regulatory T cells by blocking Foxp3 expression and activity. Nat Commun. 2016;7:10789 pubmed publisher |
- western blot; mouse; 1:1000; fig s2
| Zhou Z, Tang A, Wong W, Bamezai S, Goddard L, Shenkar R, et al. Cerebral cavernous malformations arise from endothelial gain of MEKK3-KLF2/4 signalling. Nature. 2016;532:122-6 pubmed publisher |
- immunohistochemistry - paraffin section; mouse; 1:50; fig 6
| Terakawa J, Rocchi A, Serna V, Bottinger E, Graff J, Kurita T. FGFR2IIIb-MAPK Activity Is Required for Epithelial Cell Fate Decision in the Lower Müllerian Duct. Mol Endocrinol. 2016;30:783-95 pubmed publisher |
- western blot; mouse; 1:1000; tbl 2
| Muñoz-Félix J, Pérez-Roque L, Núñez-Gómez E, Oujo B, Arevalo M, Ruiz-Remolina L, et al. Overexpression of the short endoglin isoform reduces renal fibrosis and inflammation after unilateral ureteral obstruction. Biochim Biophys Acta. 2016;: pubmed publisher |
| Rutz S, Kayagaki N, Phung Q, Eidenschenk C, Noubade R, Wang X, et al. Deubiquitinase DUBA is a post-translational brake on interleukin-17 production in T cells. Nature. 2015;518:417-21 pubmed publisher |
- chromatin immunoprecipitation; mouse; fig s14
- proximity ligation assay; mouse; fig s12
| Yoon J, Sudo K, Kuroda M, Kato M, Lee I, Han J, et al. Phosphorylation status determines the opposing functions of Smad2/Smad3 as STAT3 cofactors in TH17 differentiation. Nat Commun. 2015;6:7600 pubmed publisher |
- western blot; human; fig 1
| Aki S, Yoshioka K, Okamoto Y, Takuwa N, Takuwa Y. Phosphatidylinositol 3-kinase class II α-isoform PI3K-C2α is required for transforming growth factor β-induced Smad signaling in endothelial cells. J Biol Chem. 2015;290:6086-105 pubmed publisher |
| Wang J, Bao L, Yu B, Liu Z, Han W, Deng C, et al. Interleukin-1β Promotes Epithelial-Derived Alveolar Elastogenesis via αvβ6 Integrin-Dependent TGF-β Activation. Cell Physiol Biochem. 2015;36:2198-216 pubmed publisher |
- western blot; human; 1:1000; fig 7
| Zhou X, Tao Y, Liang C, Zhang Y, Li H, Chen Q. BMP3 Alone and Together with TGF-β Promote the Differentiation of Human Mesenchymal Stem Cells into a Nucleus Pulposus-Like Phenotype. Int J Mol Sci. 2015;16:20344-59 pubmed publisher |
| Zhang M, Zhang J, Liu S, Wang Q, Lin G, Qiu R, et al. NS5ATP9 suppresses activation of human hepatic stellate cells, possibly via inhibition of Smad3/phosphorylated-Smad3 expression. Inflammation. 2015;38:278-89 pubmed publisher |
| Cohen T, Kollias H, Liu N, Ward C, Wagner K. Genetic disruption of Smad7 impairs skeletal muscle growth and regeneration. J Physiol. 2015;593:2479-97 pubmed publisher |
| Zhang Z, Zhang T, Zhou Y, Wei X, Zhu J, Zhang J, et al. Activated phosphatidylinositol 3-kinase/Akt inhibits the transition of endothelial progenitor cells to mesenchymal cells by regulating the forkhead box subgroup O-3a signaling. Cell Physiol Biochem. 2015;35:1643-53 pubmed publisher |
- western blot; mouse; 1:1000; fig 7
| Tontonoz P, Cortez-Toledo O, Wroblewski K, Hong C, Lim L, Carranza R, et al. The orphan nuclear receptor Nur77 is a determinant of myofiber size and muscle mass in mice. Mol Cell Biol. 2015;35:1125-38 pubmed publisher |
- western blot; human; 1:1000
| Carthy J, Sundqvist A, Heldin A, van Dam H, Kletsas D, Heldin C, et al. Tamoxifen Inhibits TGF-β-Mediated Activation of Myofibroblasts by Blocking Non-Smad Signaling Through ERK1/2. J Cell Physiol. 2015;230:3084-92 pubmed publisher |
| Beaudoin M, Snook L, Arkell A, Stefanson A, Wan Z, Simpson J, et al. Novel effects of rosiglitazone on SMAD2 and SMAD3 signaling in white adipose tissue of diabetic rats. Obesity (Silver Spring). 2014;22:1632-42 pubmed publisher |
| Isogaya K, Koinuma D, Tsutsumi S, Saito R, Miyazawa K, Aburatani H, et al. A Smad3 and TTF-1/NKX2-1 complex regulates Smad4-independent gene expression. Cell Res. 2014;24:994-1008 pubmed publisher |
- western blot; mouse; 1:1000; fig 7
| Das R, Xu S, Quan X, Nguyen T, Kong I, Chung C, et al. Upregulation of mitochondrial Nox4 mediates TGF-β-induced apoptosis in cultured mouse podocytes. Am J Physiol Renal Physiol. 2014;306:F155-67 pubmed publisher |
- western blot; rat; 1:1000
| Borkham-Kamphorst E, Schaffrath C, Van De Leur E, Haas U, Tihaa L, Meurer S, et al. The anti-fibrotic effects of CCN1/CYR61 in primary portal myofibroblasts are mediated through induction of reactive oxygen species resulting in cellular senescence, apoptosis and attenuated TGF-β signaling. Biochim Biophys Acta. 2014;1843:902-14 pubmed publisher |
- western blot; rat; 1:1000
| Mitchell K, Shah J, Tsytsikova L, Campbell A, Affram K, Symes A. LPS antagonism of TGF-β signaling results in prolonged survival and activation of rat primary microglia. J Neurochem. 2014;129:155-68 pubmed publisher |
| Yi X, Li X, Zhou Y, Ren S, Wan W, Feng G, et al. Hepatocyte growth factor regulates the TGF-β1-induced proliferation, differentiation and secretory function of cardiac fibroblasts. Int J Mol Med. 2014;34:381-90 pubmed publisher |
- western blot; mouse; 1:1000
| Watatani H, Maeshima Y, Hinamoto N, Yamasaki H, Ujike H, Tanabe K, et al. Vasohibin-1 deficiency enhances renal fibrosis and inflammation after unilateral ureteral obstruction. Physiol Rep. 2014;2: pubmed publisher |
| Kapur N, Qiao X, Paruchuri V, Mackey E, Daly G, Ughreja K, et al. Reducing endoglin activity limits calcineurin and TRPC-6 expression and improves survival in a mouse model of right ventricular pressure overload. J Am Heart Assoc. 2014;3: pubmed publisher |
| Chen R, Xu B, Chen S, Chen S, Zhang T, Ren J, et al. Effect of oridonin-mediated hallmark changes on inflammatory pathways in human pancreatic cancer (BxPC-3) cells. World J Gastroenterol. 2014;20:14895-903 pubmed publisher |
| Feng Y, Wu H, Xu Y, Zhang Z, Liu T, Lin X, et al. Zinc finger protein 451 is a novel Smad corepressor in transforming growth factor-β signaling. J Biol Chem. 2014;289:2072-83 pubmed publisher |
- western blot; human; fig 1
| Holtzhausen A, Golzio C, How T, Lee Y, Schiemann W, Katsanis N, et al. Novel bone morphogenetic protein signaling through Smad2 and Smad3 to regulate cancer progression and development. FASEB J. 2014;28:1248-67 pubmed publisher |
| Ikeda Y, Ozono I, Tajima S, Imao M, Horinouchi Y, Izawa-Ishizawa Y, et al. Iron chelation by deferoxamine prevents renal interstitial fibrosis in mice with unilateral ureteral obstruction. PLoS ONE. 2014;9:e89355 pubmed publisher |
- immunohistochemistry - paraffin section; mouse
| Hall B, Wankhade U, Konkel J, Cherukuri K, Nagineni C, Flanders K, et al. Transforming growth factor-β3 (TGF-β3) knock-in ameliorates inflammation due to TGF-β1 deficiency while promoting glucose tolerance. J Biol Chem. 2013;288:32074-92 pubmed publisher |
| Maddaluno L, Rudini N, Cuttano R, Bravi L, Giampietro C, Corada M, et al. EndMT contributes to the onset and progression of cerebral cavernous malformations. Nature. 2013;498:492-6 pubmed publisher |
| Lessard S, Rivas D, Alves-Wagner A, Hirshman M, Gallagher I, Constantin-Teodosiu D, et al. Resistance to aerobic exercise training causes metabolic dysfunction and reveals novel exercise-regulated signaling networks. Diabetes. 2013;62:2717-27 pubmed publisher |
| Harazono Y, Muramatsu T, Endo H, Uzawa N, Kawano T, Harada K, et al. miR-655 Is an EMT-suppressive microRNA targeting ZEB1 and TGFBR2. PLoS ONE. 2013;8:e62757 pubmed publisher |
- western blot; mouse; fig 4a
| Yang Y, Ahn Y, Gibbons D, Zang Y, Lin W, Thilaganathan N, et al. The Notch ligand Jagged2 promotes lung adenocarcinoma metastasis through a miR-200-dependent pathway in mice. J Clin Invest. 2011;121:1373-85 pubmed publisher |
| Cigna N, Farrokhi Moshai E, Brayer S, Marchal-Sommé J, Wemeau-Stervinou L, Fabre A, et al. The hedgehog system machinery controls transforming growth factor-β-dependent myofibroblastic differentiation in humans: involvement in idiopathic pulmonary fibrosis. Am J Pathol. 2012;181:2126-37 pubmed publisher |
| Egerman M, Cadena S, Gilbert J, Meyer A, Nelson H, Swalley S, et al. GDF11 Increases with Age and Inhibits Skeletal Muscle Regeneration. Cell Metab. 2015;22:164-74 pubmed publisher |
| Wylie-Sears J, Levine R, Bischoff J. Losartan inhibits endothelial-to-mesenchymal transformation in mitral valve endothelial cells by blocking transforming growth factor-β-induced phosphorylation of ERK. Biochem Biophys Res Commun. 2014;446:870-5 pubmed publisher |
| Wang Y, Ho C, Bang E, Rejon C, Libasci V, Pertchenko P, et al. Bone morphogenetic protein 2 stimulates noncanonical SMAD2/3 signaling via the BMP type 1A receptor in gonadotrope-like cells: implications for FSH synthesis. Endocrinology. 2014;155:1970-81 pubmed publisher |
| Fabre T, Kared H, Friedman S, Shoukry N. IL-17A enhances the expression of profibrotic genes through upregulation of the TGF-β receptor on hepatic stellate cells in a JNK-dependent manner. J Immunol. 2014;193:3925-33 pubmed publisher |
| Bañón-Maneus E, Rovira J, Ramírez-Bajo M, Moya-Rull D, Hierro-Garcia N, Takenaka S, et al. Wnt pathway activation in long term remnant rat model. Biomed Res Int. 2014;2014:324713 pubmed publisher |
| Li Y, Klausen C, Cheng J, Zhu H, Leung P. Activin A, B, and AB increase human trophoblast cell invasion by up-regulating N-cadherin. J Clin Endocrinol Metab. 2014;99:E2216-25 pubmed publisher |
| Chen B, Wang W, Shen T, Qi R. Thioredoxin1 downregulates oxidized low-density lipoprotein-induced adhesion molecule expression via Smad3 protein. PLoS ONE. 2013;8:e76226 pubmed publisher |
| Zimmerman K, Graham L, Pallero M, Murphy-Ullrich J. Calreticulin regulates transforming growth factor-β-stimulated extracellular matrix production. J Biol Chem. 2013;288:14584-98 pubmed publisher |
| Guo Z, Khattar M, Schroder P, Miyahara Y, Wang G, He X, et al. A dynamic dual role of IL-2 signaling in the two-step differentiation process of adaptive regulatory T cells. J Immunol. 2013;190:3153-62 pubmed publisher |
| Maurya V, Jha R, Kumar V, Joshi A, Chadchan S, Mohan J, et al. Transforming growth factor-beta 1 (TGF-B1) liberation from its latent complex during embryo implantation and its regulation by estradiol in mouse. Biol Reprod. 2013;89:84 pubmed publisher |
| Nakamura K, Nakano S, Miyoshi T, Yamanouchi K, Nishihara M. Loss of SPARC in mouse skeletal muscle causes myofiber atrophy. Muscle Nerve. 2013;48:791-9 pubmed publisher |
| Nelson E, Huang C, Ewel J, Chang A, Yuan C. Halofuginone down-regulates Smad3 expression and inhibits the TGFbeta-induced expression of fibrotic markers in human corneal fibroblasts. Mol Vis. 2012;18:479-87 pubmed |
| Bergstrom R, Savary K, Morén A, Guibert S, Heldin C, Ohlsson R, et al. Transforming growth factor beta promotes complexes between Smad proteins and the CCCTC-binding factor on the H19 imprinting control region chromatin. J Biol Chem. 2010;285:19727-37 pubmed publisher |
| Zollo M, Di Dato V, Spano D, De Martino D, Liguori L, Marino N, et al. Targeting monocyte chemotactic protein-1 synthesis with bindarit induces tumor regression in prostate and breast cancer animal models. Clin Exp Metastasis. 2012;29:585-601 pubmed publisher |
| Louafi F, Martinez-Nunez R, Sanchez-Elsner T. MicroRNA-155 targets SMAD2 and modulates the response of macrophages to transforming growth factor-{beta}. J Biol Chem. 2010;285:41328-36 pubmed publisher |
