product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb
catalog :
9516
clonality :
monoclonal
host :
rabbit
conjugate :
nonconjugated
antigen modification :
phosphorylated
clone name :
41D10
reactivity :
human, mouse, rat, fruit fly
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - free floating section
citations: 53
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; mouse; 1:100; loading ...; fig 5d
Diéguez Hurtado R, Kato K, Giaimo B, Nieminen Kelhä M, Arf H, Ferrante F, et al. Loss of the transcription factor RBPJ induces disease-promoting properties in brain pericytes. Nat Commun. 2019;10:2817 pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 3c
Dube P, Chikkamenahalli L, Birnbaumer L, Vazquez G. Reduced calcification and osteogenic features in advanced atherosclerotic plaques of mice with macrophage-specific loss of TRPC3. Atherosclerosis. 2018;270:199-204 pubmed publisher
  • western blot; human; loading ...; fig 1e
Ventura E, Weller M, Burghardt I. Cutting Edge: ERK1 Mediates the Autocrine Positive Feedback Loop of TGF-? and Furin in Glioma-Initiating Cells. J Immunol. 2017;198:4569-4574 pubmed publisher
  • immunohistochemistry; fruit fly; 1:200; loading ...; fig s3b
Tang Y, Geng Q, Chen D, Zhao S, Liu X, Wang Z. Germline Proliferation Is Regulated by Somatic Endocytic Genes via JNK and BMP Signaling in Drosophila. Genetics. 2017;206:189-197 pubmed publisher
  • western blot; rat; 1:1000; fig 8a
Shu D, Wojciechowski M, Lovicu F. Bone Morphogenetic Protein-7 Suppresses TGF?2-Induced Epithelial-Mesenchymal Transition in the Lens: Implications for Cataract Prevention. Invest Ophthalmol Vis Sci. 2017;58:781-796 pubmed publisher
  • western blot; human; 1:1000; fig 1h
Hurst L, Dunmore B, Long L, Crosby A, Al Lamki R, Deighton J, et al. TNFα drives pulmonary arterial hypertension by suppressing the BMP type-II receptor and altering NOTCH signalling. Nat Commun. 2017;8:14079 pubmed publisher
  • western blot; mouse; loading ...; fig s5a
Jiang C, Diao F, Sang Y, Xu N, Zhu R, Wang X, et al. GGPP-Mediated Protein Geranylgeranylation in Oocyte Is Essential for the Establishment of Oocyte-Granulosa Cell Communication and Primary-Secondary Follicle Transition in Mouse Ovary. PLoS Genet. 2017;13:e1006535 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 6e
  • western blot; human; loading ...; fig 6d
Sun J, Liu X, Gao H, Zhang L, Ji Q, Wang Z, et al. Overexpression of colorectal cancer oncogene CHRDL2 predicts a poor prognosis. Oncotarget. 2017;8:11489-11506 pubmed publisher
  • western blot; human; fig 2c
Iyer S, Chhabra Y, Harvey T, Wang R, Chiu H, Smith A, et al. CRIM1 is necessary for coronary vascular endothelial cell development and homeostasis. J Mol Histol. 2017;48:53-61 pubmed publisher
  • immunocytochemistry; human; 1:500; loading ...; fig 2c
  • western blot; human; 1:1000; loading ...; fig 2e
Yu Z, Mouillesseaux K, Kushner E, Bautch V. Tumor-Derived Factors and Reduced p53 Promote Endothelial Cell Centrosome Over-Duplication. PLoS ONE. 2016;11:e0168334 pubmed publisher
  • western blot; human; loading ...; fig 11A
Yuan H, Sehgal P. MxA Is a Novel Regulator of Endosome-Associated Transcriptional Signaling by Bone Morphogenetic Proteins 4 and 9 (BMP4 and BMP9). PLoS ONE. 2016;11:e0166382 pubmed publisher
  • immunocytochemistry; human; 1:1000; loading ...; fig 1h
Mouillesseaux K, Wiley D, Saunders L, Wylie L, Kushner E, Chong D, et al. Notch regulates BMP responsiveness and lateral branching in vessel networks via SMAD6. Nat Commun. 2016;7:13247 pubmed publisher
  • western blot; mouse; fig 8
Zhao G, Zhu P, Renvoisé B, Maldonado Baez L, Park S, Blackstone C. Mammalian knock out cells reveal prominent roles for atlastin GTPases in ER network morphology. Exp Cell Res. 2016;349:32-44 pubmed publisher
  • western blot; mouse; 1:1000; fig 5
Liu W, Zhou L, Zhou C, Zhang S, Jing J, Xie L, et al. GDF11 decreases bone mass by stimulating osteoclastogenesis and inhibiting osteoblast differentiation. Nat Commun. 2016;7:12794 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:50; loading ...; fig 6h
Gerling M, Büller N, Kirn L, Joost S, Frings O, Englert B, et al. Stromal Hedgehog signalling is downregulated in colon cancer and its restoration restrains tumour growth. Nat Commun. 2016;7:12321 pubmed publisher
  • immunohistochemistry; mouse; 1:50; loading ...; fig 6c
Morgan E, Pittman J, DeGiacomo A, Cusher D, de Bakker C, Mroszczyk K, et al. BMPR1A antagonist differentially affects cartilage and bone formation during fracture healing. J Orthop Res. 2016;34:2096-2105 pubmed publisher
  • western blot; human; loading ...; fig 3a
Kurimoto R, Iwasawa S, Ebata T, Ishiwata T, Sekine I, Tada Y, et al. Drug resistance originating from a TGF-β/FGF-2-driven epithelial-to-mesenchymal transition and its reversion in human lung adenocarcinoma cell lines harboring an EGFR mutation. Int J Oncol. 2016;48:1825-36 pubmed publisher
  • western blot; human; fig 1
Micha D, Voermans E, Eekhoff M, van Essen H, Zandieh Doulabi B, Netelenbos C, et al. Inhibition of TGFβ signaling decreases osteogenic differentiation of fibrodysplasia ossificans progressiva fibroblasts in a novel in vitro model of the disease. Bone. 2016;84:169-180 pubmed publisher
  • western blot; mouse; fig 4b
Cuttano R, Rudini N, Bravi L, Corada M, Giampietro C, Papa E, et al. KLF4 is a key determinant in the development and progression of cerebral cavernous malformations. EMBO Mol Med. 2016;8:6-24 pubmed publisher
  • western blot; human; 1:1000; fig 4c
Massouridès E, Polentes J, Mangeot P, Mournetas V, Nectoux J, Deburgrave N, et al. Dp412e: a novel human embryonic dystrophin isoform induced by BMP4 in early differentiated cells. Skelet Muscle. 2015;5:40 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:200; fig 1
  • western blot; mouse; 1:1000; fig s4
Pickup M, Hover L, Guo Y, Gorska A, Chytil A, Novitskiy S, et al. Deletion of the BMP receptor BMPR1a impairs mammary tumor formation and metastasis. Oncotarget. 2015;6:22890-904 pubmed
  • western blot; rat; 1:1000; fig 8
Sun J, Li J, Li C, Yu Y. Role of bone morphogenetic protein-2 in osteogenic differentiation of mesenchymal stem cells. Mol Med Rep. 2015;12:4230-4237 pubmed publisher
  • western blot; human; 1:1000; fig s1
Krishnan S, Szabo E, Burghardt I, Frei K, Tabatabai G, Weller M. Modulation of cerebral endothelial cell function by TGF-β in glioblastoma: VEGF-dependent angiogenesis versus endothelial mesenchymal transition. Oncotarget. 2015;6:22480-95 pubmed
  • western blot; human; 1:1000; fig 6
Alkhateeb A, Buckett P, Gardeck A, Kim J, Byrne S, Fraenkel P, et al. The small molecule ferristatin II induces hepatic hepcidin expression in vivo and in vitro. Am J Physiol Gastrointest Liver Physiol. 2015;308:G1019-26 pubmed publisher
  • western blot; human
Perna F, Vu L, Themeli M, Kriks S, Hoya Arias R, Khanin R, et al. The polycomb group protein L3MBTL1 represses a SMAD5-mediated hematopoietic transcriptional program in human pluripotent stem cells. Stem Cell Reports. 2015;4:658-69 pubmed publisher
  • western blot; mouse; 1:1000
Tian E, Stevens S, Guan Y, Springer D, Anderson S, Starost M, et al. Galnt1 is required for normal heart valve development and cardiac function. PLoS ONE. 2015;10:e0115861 pubmed publisher
  • western blot; mouse; fig 5
Malhotra R, Burke M, Martyn T, Shakartzi H, Thayer T, O Rourke C, et al. Inhibition of bone morphogenetic protein signal transduction prevents the medial vascular calcification associated with matrix Gla protein deficiency. PLoS ONE. 2015;10:e0117098 pubmed publisher
  • immunohistochemistry - paraffin section; rat; 1:100
Hollis E, Ishiko N, Tolentino K, Doherty E, Rodríguez M, Calcutt N, et al. A novel and robust conditioning lesion induced by ethidium bromide. Exp Neurol. 2015;265:30-9 pubmed publisher
  • western blot; mouse
Galvagni F, Lentucci C, Neri F, Dettori D, De Clemente C, Orlandini M, et al. Snai1 promotes ESC exit from the pluripotency by direct repression of self-renewal genes. Stem Cells. 2015;33:742-50 pubmed publisher
  • western blot; mouse
Kim J, Shim M. Prostaglandin F2α receptor (FP) signaling regulates Bmp signaling and promotes chondrocyte differentiation. Biochim Biophys Acta. 2015;1853:500-12 pubmed publisher
  • immunocytochemistry; mouse; 1:50
Chung D, Gao F, Jegga A, Das S. Estrogen mediated epithelial proliferation in the uterus is directed by stromal Fgf10 and Bmp8a. Mol Cell Endocrinol. 2015;400:48-60 pubmed publisher
  • immunohistochemistry; rat; 1:1500; fig 4
Scholze A, Foo L, Mulinyawe S, Barres B. BMP signaling in astrocytes downregulates EGFR to modulate survival and maturation. PLoS ONE. 2014;9:e110668 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:50; fig s5
Cui C, Yin M, Sima J, Childress V, Michel M, Piao Y, et al. Involvement of Wnt, Eda and Shh at defined stages of sweat gland development. Development. 2014;141:3752-60 pubmed publisher
  • immunohistochemistry - paraffin section; mouse
Gu S, Wu W, Liu C, Yang L, Sun C, Ye W, et al. BMPRIA mediated signaling is essential for temporomandibular joint development in mice. PLoS ONE. 2014;9:e101000 pubmed publisher
  • immunocytochemistry; mouse; fig s1
Onishi K, Tonge P, Nagy A, Zandstra P. Local BMP-SMAD1 signaling increases LIF receptor-dependent STAT3 responsiveness and primed-to-naive mouse pluripotent stem cell conversion frequency. Stem Cell Reports. 2014;3:156-68 pubmed publisher
  • western blot; mouse; fig 6
Dai X, Jiang W, Zhang Q, Xu L, Geng P, Zhuang S, et al. Requirement for integrin-linked kinase in neural crest migration and differentiation and outflow tract morphogenesis. BMC Biol. 2013;11:107 pubmed publisher
  • western blot; human; 1:2000
Ren G, Beech C, Smas C. The immunoglobulin superfamily protein differentiation of embryonic stem cells 1 (dies1) has a regulatory role in preadipocyte to adipocyte conversion. PLoS ONE. 2013;8:e65531 pubmed publisher
  • western blot; mouse
Maddaluno L, Rudini N, Cuttano R, Bravi L, Giampietro C, Corada M, et al. EndMT contributes to the onset and progression of cerebral cavernous malformations. Nature. 2013;498:492-6 pubmed publisher
  • western blot; human
Sakaki Yumoto M, Liu J, Ramalho Santos M, Yoshida N, Derynck R. Smad2 is essential for maintenance of the human and mouse primed pluripotent stem cell state. J Biol Chem. 2013;288:18546-60 pubmed publisher
  • immunohistochemistry - free floating section; mouse; 1:50
  • western blot; mouse; 1:1000
Tong K, Kwan K. Common partner Smad-independent canonical bone morphogenetic protein signaling in the specification process of the anterior rhombic lip during cerebellum development. Mol Cell Biol. 2013;33:1925-37 pubmed publisher
Vuilleumier R, Lian T, Flibotte S, Khan Z, Fuchs A, Pyrowolakis G, et al. Retrograde BMP signaling activates neuronal gene expression through widespread deployment of a conserved BMP-responsive cis-regulatory activation element. Nucleic Acids Res. 2019;47:679-699 pubmed publisher
Tang Y, Tang L, Xu X, Li C, Deng C, Zhang Y. Generation of Smurf2 Conditional Knockout Mice. Int J Biol Sci. 2018;14:542-548 pubmed publisher
Dube P, Birnbaumer L, Vazquez G. Evidence for constitutive bone morphogenetic protein-2 secretion by M1 macrophages: Constitutive auto/paracrine osteogenic signaling by BMP-2 in M1 macrophages. Biochem Biophys Res Commun. 2017;491:154-158 pubmed publisher
Nasrallah R, Knezevic K, Thai T, Thomas S, Göttgens B, Lacaud G, et al. Endoglin potentiates nitric oxide synthesis to enhance definitive hematopoiesis. Biol Open. 2015;4:819-29 pubmed publisher
de la Croix Ndong J, Makowski A, Uppuganti S, Vignaux G, Ono K, Perrien D, et al. Asfotase-? improves bone growth, mineralization and strength in mouse models of neurofibromatosis type-1. Nat Med. 2014;20:904-10 pubmed publisher
Yeung C, Gossan N, Lu Y, Hughes A, Hensman J, Bayer M, et al. Gremlin-2 is a BMP antagonist that is regulated by the circadian clock. Sci Rep. 2014;4:5183 pubmed publisher
Weeks O, Bhullar B, Abzhanov A. Molecular characterization of dental development in a toothed archosaur, the American alligator Alligator mississippiensis. Evol Dev. 2013;15:393-405 pubmed publisher
Wu Y, Ai Z, Yao K, Cao L, Du J, Shi X, et al. CHIR99021 promotes self-renewal of mouse embryonic stem cells by modulation of protein-encoding gene and long intergenic non-coding RNA expression. Exp Cell Res. 2013;319:2684-99 pubmed publisher
Steensma M, Tyler W, Shaber A, Goldring S, Ross F, Williams B, et al. Targeting the giant cell tumor stromal cell: functional characterization and a novel therapeutic strategy. PLoS ONE. 2013;8:e69101 pubmed publisher
Vrijens K, Lin W, Cui J, Farmer D, Low J, Pronier E, et al. Identification of small molecule activators of BMP signaling. PLoS ONE. 2013;8:e59045 pubmed publisher
Takahashi A, Morita M, Yokoyama K, Suzuki T, Yamamoto T. Tob2 inhibits peroxisome proliferator-activated receptor ?2 expression by sequestering Smads and C/EBP? during adipocyte differentiation. Mol Cell Biol. 2012;32:5067-77 pubmed publisher
Ng K, Lee Y, Lai W, Chan Y, Fung M, Tse H, et al. Exogenous expression of human apoA-I enhances cardiac differentiation of pluripotent stem cells. PLoS ONE. 2011;6:e19787 pubmed publisher
Kosinski C, Stange D, Xu C, Chan A, Ho C, Yuen S, et al. Indian hedgehog regulates intestinal stem cell fate through epithelial-mesenchymal interactions during development. Gastroenterology. 2010;139:893-903 pubmed publisher
product information
SKU :
9516S
Product-Name :
Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb
Size :
100 ul
Price-(USD) :
274 USD
Species-x-Reactivity :
H, M, R
Applications :
Flow cytometry
Product-Category :
Developmental Biology
Shipping-Temp :
COLD
Storage-Temp :
-20°C
Product-Type :
Monoclonal Antibody
MW :
60
Host :
Rabbit
Target :
Smad1 (Ser463/Ser465) phosphate/Smad5 (Ser463/Ser465) phosphate
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
https://www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.