product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb
catalog :
9496S
quantity :
100 ul
price :
274 USD
clonality :
monoclonal
host :
rabbit
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
citations: 38
Published Application/Species/DilutionReference
  • immunohistochemistry - paraffin section; human; 1:50; fig 7
  • western blot; human; 1:1000; fig s15
Nakazawa S, Oikawa D, Ishii R, Ayaki T, Takahashi H, Takeda H, et al. Linear ubiquitination is involved in the pathogenesis of optineurin-associated amyotrophic lateral sclerosis. Nat Commun. 2016;7:12547 pubmed publisher
  • western blot; human; fig 5
Gao X, Feng J, He Y, Xu F, Fan X, Huang W, et al. hnRNPK inhibits GSK3β Ser9 phosphorylation, thereby stabilizing c-FLIP and contributes to TRAIL resistance in H1299 lung adenocarcinoma cells. Sci Rep. 2016;6:22999 pubmed publisher
  • western blot; human; 1:500; tbl he primary antibodies and dilutions used were as follows: anti-human HSP27 (1:1,000, #ab1426; Abcam, Cambridge, UK), anti-HSP90 (1:1,000, #ab54285; Abcam, Cambridge, UK), anti-HSP70 (1:1000, #ab47455; Abcam, Cambridge, UK), anti-HSPA5/GRP78 (1:1000, # ab137404; Abcam, Cambridge, UK), anti-caspase 3 (1:1,000, #9662S; Cell Signaling, Danvers, MA, USA), anti-Nanog (1:2,000, #AB9220; Chemicon/Merck Millipore, Billerica, MA, USA), anti-SOX2 (1:1,000, #20118-1-AP; Proteintech, Chicago, IL, USA), anti-c-myc (1:1000, # ab51154; Abcam, Cambridge, UK), anti-caspase 9 (1:1,000, #9502; Cell Signaling, Danvers, MA, USA), anti-PARP-1 (1:1,000, #9542; Cell Signaling, Danvers, MA, USA), anti-caspase 6 (1:1,000, #9762; Cell Signaling, Danvers, MA, USA), anti-caspase 8 (1:500, #9496; Cell Signaling, Danvers, MA, USA), and anti-GAPDH (1:2,000, #AM4300; Ambion/Life Technologies, Austin, TX, USA).
Cheng Y, Huang C, Lee Y, Tien L, Ku W, Chien R, et al. Knocking down of heat-shock protein 27 directs differentiation of functional glutamatergic neurons from placenta-derived multipotent cells. Sci Rep. 2016;6:30314 pubmed publisher
  • western blot; human; fig 2
Helmke C, Raab M, Rodel F, Matthess Y, Oellerich T, Mandal R, et al. Ligand stimulation of CD95 induces activation of Plk3 followed by phosphorylation of caspase-8. Cell Res. 2016;26:914-34 pubmed publisher
  • western blot; human; fig 2
Choi H, Kim M, Choi Y, Shin Y, Cho S, Ko S. Rhus verniciflua Stokes (RVS) and butein induce apoptosis of paclitaxel-resistant SKOV-3/PAX ovarian cancer cells through inhibition of AKT phosphorylation. BMC Complement Altern Med. 2016;16:122 pubmed publisher
  • western blot; human; fig 5
Natarelli L, Ranaldi G, Leoni G, Roselli M, Guantario B, Comitato R, et al. Nanomolar Caffeic Acid Decreases Glucose Uptake and the Effects of High Glucose in Endothelial Cells. PLoS ONE. 2015;10:e0142421 pubmed publisher
  • western blot; human
Shen X, Sun W, Shi Y, Xing Z, Su X. Altered viral replication and cell responses by inserting microRNA recognition element into PB1 in pandemic influenza A virus (H1N1) 2009. Mediators Inflamm. 2015;2015:976575 pubmed publisher
  • western blot; human; 1:1000; fig 8A
Silva V, Plooster M, Leung J, Cassimeris L. A delay prior to mitotic entry triggers caspase 8-dependent cell death in p53-deficient Hela and HCT-116 cells. Cell Cycle. 2015;14:1070-81 pubmed publisher
  • western blot; human
Li X, Liang Q, Liu W, Zhang N, Xu L, Zhang X, et al. Ras association domain family member 10 suppresses gastric cancer growth by cooperating with GSTP1 to regulate JNK/c-Jun/AP-1 pathway. Oncogene. 2015;: pubmed publisher
  • western blot; human; fig 3
Long J, Schoonen P, Graczyk D, O'Prey J, Ryan K. p73 engages A2B receptor signalling to prime cancer cells to chemotherapy-induced death. Oncogene. 2015;34:5152-62 pubmed publisher
  • western blot; human
Yanagi T, Shi R, Aza-Blanc P, Reed J, Matsuzawa S. PCTAIRE1-knockdown sensitizes cancer cells to TNF family cytokines. PLoS ONE. 2015;10:e0119404 pubmed publisher
  • western blot; human; fig 5
Shen W, Chang A, Wang J, Zhou W, Gao R, Li J, et al. TIFA, an inflammatory signaling adaptor, is tumor suppressive for liver cancer. Oncogenesis. 2015;4:e173 pubmed publisher
  • western blot; human; fig 3
Yang L, Li Y, Bhattacharya A, Zhang Y. Inhibition of ERBB2-overexpressing Tumors by Recombinant Human Prolidase and Its Enzymatically Inactive Mutant. EBioMedicine. 2015;2:396-405 pubmed
  • western blot; human
Chopra A, Anderson A, Giardina C. Novel piperazine-based compounds inhibit microtubule dynamics and sensitize colon cancer cells to tumor necrosis factor-induced apoptosis. J Biol Chem. 2014;289:2978-91 pubmed publisher
  • western blot; human
Matthess Y, Raab M, Knecht R, Becker S, Strebhardt K. Sequential Cdk1 and Plk1 phosphorylation of caspase-8 triggers apoptotic cell death during mitosis. Mol Oncol. 2014;8:596-608 pubmed publisher
  • western blot; human
Derangère V, Chevriaux A, Courtaut F, Bruchard M, Berger H, Chalmin F, et al. Liver X receptor β activation induces pyroptosis of human and murine colon cancer cells. Cell Death Differ. 2014;21:1914-24 pubmed publisher
  • flow cytometry; human
  • western blot; human
Villa-Morales M, Cobos M, González-Gugel E, Alvarez-Iglesias V, Martinez B, Piris M, et al. FAS system deregulation in T-cell lymphoblastic lymphoma. Cell Death Dis. 2014;5:e1110 pubmed publisher
  • western blot; human; 1:1000
Ishikawa K, Saiki S, Furuya N, Yamada D, Imamichi Y, Li Y, et al. P150glued-associated disorders are caused by activation of intrinsic apoptotic pathway. PLoS ONE. 2014;9:e94645 pubmed publisher
  • western blot; human
  • immunocytochemistry; human
Sung M, Giannakakou P. BRCA1 regulates microtubule dynamics and taxane-induced apoptotic cell signaling. Oncogene. 2014;33:1418-28 pubmed publisher
  • western blot; human; 1:10; fig 4
Kang C, Lin J, Xu Z, Kumar S, Herr A. Single-cell Western blotting after whole-cell imaging to assess cancer chemotherapeutic response. Anal Chem. 2014;86:10429-36 pubmed publisher
  • western blot; human
Zhang S, Tang W, Weng S, Liu X, Rao B, Gu J, et al. Apollon modulates chemosensitivity in human esophageal squamous cell carcinoma. Oncotarget. 2014;5:7183-97 pubmed
  • flow cytometry; human
Pavet V, Shlyakhtina Y, He T, Ceschin D, Kohonen P, Perala M, et al. Plasminogen activator urokinase expression reveals TRAIL responsiveness and supports fractional survival of cancer cells. Cell Death Dis. 2014;5:e1043 pubmed publisher
  • western blot; human
Han J, Soletti R, Sadarangani A, Sridevi P, Ramirez M, Eckmann L, et al. Nuclear expression of β-catenin promotes RB stability and resistance to TNF-induced apoptosis in colon cancer cells. Mol Cancer Res. 2013;11:207-18 pubmed publisher
  • western blot; human
Linderoth E, Pilia G, Mahajan N, Ferby I. Activated Cdc42-associated kinase 1 (Ack1) is required for tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) receptor recruitment to lipid rafts and induction of cell death. J Biol Chem. 2013;288:32922-31 pubmed publisher
  • western blot; human; 1:1000
Giansanti V, Rodriguez G, Savoldelli M, Gioia R, Forlino A, Mazzini G, et al. Characterization of stress response in human retinal epithelial cells. J Cell Mol Med. 2013;17:103-15 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:100
  • western blot; human; 1:1000
Gauster M, Siwetz M, Huppertz B. Fusion of villous trophoblast can be visualized by localizing active caspase 8. Placenta. 2009;30:547-50 pubmed publisher
Franco R, Bortner C, Schmitz I, Cidlowski J. Glutathione depletion regulates both extrinsic and intrinsic apoptotic signaling cascades independent from multidrug resistance protein 1. Apoptosis. 2014;19:117-34 pubmed publisher
Shu Q, Li W, Li H, Sun G. Vasostatin inhibits VEGF-induced endothelial cell proliferation, tube formation and induces cell apoptosis under oxygen deprivation. Int J Mol Sci. 2014;15:6019-30 pubmed publisher
Peter B, Cerny-Reiterer S, Hadzijusufovic E, Schuch K, Stefanzl G, Eisenwort G, et al. The pan-Bcl-2 blocker obatoclax promotes the expression of Puma, Noxa, and Bim mRNA and induces apoptosis in neoplastic mast cells. J Leukoc Biol. 2014;95:95-104 pubmed publisher
Foreman K, Jesse J, Kuo P, Gupta G. Emetine dihydrochloride: a novel therapy for bladder cancer. J Urol. 2014;191:502-9 pubmed publisher
Gao X, Lin J, Ning Q, Gao L, Yao Y, Zhou J, et al. A histone acetyltransferase p300 inhibitor C646 induces cell cycle arrest and apoptosis selectively in AML1-ETO-positive AML cells. PLoS ONE. 2013;8:e55481 pubmed publisher
Biton S, Ashkenazi A. NEMO and RIP1 control cell fate in response to extensive DNA damage via TNF-α feedforward signaling. Cell. 2011;145:92-103 pubmed publisher
Ghanim V, Herrmann H, Heller G, Peter B, Hadzijusufovic E, Blatt K, et al. 5-azacytidine and decitabine exert proapoptotic effects on neoplastic mast cells: role of FAS-demethylation and FAS re-expression, and synergism with FAS-ligand. Blood. 2012;119:4242-52 pubmed publisher
D'Agostino L, Giordano A. A novel dual signaling axis for NSP 5a3a induced apoptosis in head and neck carcinoma. Oncotarget. 2011;2:1055-74 pubmed
Thomé C, Dos Santos G, Ferreira G, Scheucher P, Izumi C, Leopoldino A, et al. Linker for activation of T-cell family member2 (LAT2) a lipid raft adaptor protein for AKT signaling, is an early mediator of alkylphospholipid anti-leukemic activity. Mol Cell Proteomics. 2012;11:1898-912 pubmed publisher
Stutz N, Johnson R, Wood G. The Fas apoptotic pathway in cutaneous T-cell lymphomas: frequent expression of phenotypes associated with resistance to apoptosis. J Am Acad Dermatol. 2012;67:1327.e1-10 pubmed publisher
Henrich C, Thomas C, Brooks A, Booth N, Lowery E, Pompei R, et al. Effects of cucurbitacins on cell morphology are associated with sensitization of renal carcinoma cells to TRAIL-induced apoptosis. Apoptosis. 2012;17:79-89 pubmed publisher
Jinesh G G, Chunduru S, Kamat A. Smac mimetic enables the anticancer action of BCG-stimulated neutrophils through TNF-α but not through TRAIL and FasL. J Leukoc Biol. 2012;92:233-44 pubmed publisher
product information
SKU :
9496S
Product-Name :
Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb
Size :
100 ul
Price-(USD) :
274 USD
Species-x-Reactivity :
H
Applications :
Flow cytometry
Product-Category :
Apoptosis
Shipping-Temp :
AMBIENT
Storage-Temp :
-20°C
Product-Type :
Monoclonal Antibody
MW :
18, 41, 43
Host :
Rabbit
Target :
Casp8 (Asp374) cleaved
Primary-Protein :
Casp8
Alt-Names :
ALPS2B,Apoptotic cysteine protease,Apoptotic protease Mch-5,CAP4,CASP-8,CASP8,Caspase-8,Caspase-8 subunit p10,Caspase-8 subunit p18,FADD-homologous ICE/ced-3-like protease,FADD-like ICE,FLICE,FLJ17672,ICE-like apoptotic protease 5,MACH,MACH-alpha-1/2/3 protein,MACH-beta-1/2/3/4 protein,MCH5,MGC78473,MORT1-associated ced-3 homolog,caspase 8, apoptosis-related cysteine peptidase,caspase 8, apoptosis-related cysteine protease
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.