| Published Application/Species/Sample/Dilution | Reference |
|---|
- western blot; mouse; 1:500; loading ...; fig 6a
| Barbé C, Loumaye A, Lause P, Ritvos O, Thissen J. p21-Activated Kinase 1 Is Permissive for the Skeletal Muscle Hypertrophy Induced by Myostatin Inhibition. Front Physiol. 2021;12:677746 pubmed publisher |
- western blot; mouse; 1:1000; loading ...; fig 3e
| Yang C, Liu Q, Chen Y, Wang X, Ran Z, Fang F, et al. Melatonin delays ovarian aging in mice by slowing down the exhaustion of ovarian reserve. Commun Biol. 2021;4:534 pubmed publisher |
- western blot; human; 1:1000; loading ...; fig 3e
| Zhang J, Hu J, Li W, Zhang C, Su P, Wang Y, et al. Rapamycin Antagonizes BCRP-Mediated Drug Resistance Through the PI3K/Akt/mTOR Signaling Pathway in mPRα-Positive Breast Cancer. Front Oncol. 2021;11:608570 pubmed publisher |
- western blot; pigs ; loading ...; fig 4a
| Tian M, Chen J, Wu Z, Song H, Yang F, Cui C, et al. Fat Encapsulation Reduces Diarrhea in Piglets Partially by Repairing the Intestinal Barrier and Improving Fatty Acid Transport. Animals (Basel). 2020;11: pubmed publisher |
- western blot; human; loading ...; fig 2d
| Bao Y, Oguz G, Lee W, Lee P, Ghosh K, Li J, et al. EZH2-mediated PP2A inactivation confers resistance to HER2-targeted breast cancer therapy. Nat Commun. 2020;11:5878 pubmed publisher |
- immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 4a
- immunocytochemistry; mouse; 1:100; loading ...; fig 3g
- western blot; human; 1:1000; loading ...; fig 1a
| Ruan H, Li X, Xu X, Leibowitz B, Tong J, Chen L, et al. eIF4E S209 phosphorylation licenses myc- and stress-driven oncogenesis. elife. 2020;9: pubmed publisher |
- western blot; human; 1:1000; loading ...; fig 6a
| Silva M, Nandi G, Tentarelli S, Gurrell I, Jamier T, Lucente D, et al. Prolonged tau clearance and stress vulnerability rescue by pharmacological activation of autophagy in tauopathy neurons. Nat Commun. 2020;11:3258 pubmed publisher |
- western blot; human; loading ...; fig s3c
| Koundouros N, Karali E, Tripp A, Valle A, Inglese P, Perry N, et al. Metabolic Fingerprinting Links Oncogenic PIK3CA with Enhanced Arachidonic Acid-Derived Eicosanoids. Cell. 2020;181:1596-1611.e27 pubmed publisher |
- western blot; mouse; 1:1000; loading ...; fig 7c
| Srikuea R, Hirunsai M, Charoenphandhu N. Regulation of vitamin D system in skeletal muscle and resident myogenic stem cell during development, maturation, and ageing. Sci Rep. 2020;10:8239 pubmed publisher |
- western blot; human; 1:1000; loading ...; fig 7b
| Arc Chagnaud C, Py G, Fovet T, Roumanille R, Demangel R, Pagano A, et al. Evaluation of an Antioxidant and Anti-inflammatory Cocktail Against Human Hypoactivity-Induced Skeletal Muscle Deconditioning. Front Physiol. 2020;11:71 pubmed publisher |
- western blot; human; loading ...; fig 5d
| Xu Q, Ge Q, Zhou Y, Yang B, Yang Q, Jiang S, et al. MELK promotes Endometrial carcinoma progression via activating mTOR signaling pathway. EBioMedicine. 2020;51:102609 pubmed publisher |
- western blot; human; 1:1000; loading ...; fig 1a
| Wang H, Huang F, Zhang Z, Wang P, Luo Y, Li H, et al. Feedback Activation of SGK3 and AKT Contributes to Rapamycin Resistance by Reactivating mTORC1/4EBP1 Axis via TSC2 in Breast Cancer. Int J Biol Sci. 2019;15:929-941 pubmed publisher |
- immunohistochemistry; mouse; loading ...; fig 1a
| William M, Leroux L, Chaparro V, Graber T, Alain T, Jaramillo M. Translational repression of Ccl5 and Cxcl10 by 4E-BP1 and 4E-BP2 restrains the ability of mouse macrophages to induce migration of activated T cells. Eur J Immunol. 2019;49:1200-1212 pubmed publisher |
- western blot; mouse; loading ...; fig s2d
| Wu J, Ma S, Sandhoff R, Ming Y, Hotz Wagenblatt A, Timmerman V, et al. Loss of Neurological Disease HSAN-I-Associated Gene SPTLC2 Impairs CD8+ T Cell Responses to Infection by Inhibiting T Cell Metabolic Fitness. Immunity. 2019;50:1218-1231.e5 pubmed publisher |
- western blot; mouse; loading ...; fig 4a
| Wang S, Ni H, Chao X, Wang H, Bridges B, Kumer S, et al. Impaired TFEB-mediated lysosomal biogenesis promotes the development of pancreatitis in mice and is associated with human pancreatitis. Autophagy. 2019;15:1954-1969 pubmed publisher |
- western blot; rat; 1:1000; loading ...; fig 2b
- western blot; mouse; 1:1000; loading ...; fig 1a, 2e
| Ranek M, Kokkonen Simon K, Chen A, Dunkerly Eyring B, Vera M, Oeing C, et al. PKG1-modified TSC2 regulates mTORC1 activity to counter adverse cardiac stress. Nature. 2019;566:264-269 pubmed publisher |
- western blot; human; 1:3000; loading ...; fig s4a
| NGUYEN J, Ray C, Fox A, Mendonça D, Kim J, Krebsbach P. Mammalian EAK-7 activates alternative mTOR signaling to regulate cell proliferation and migration. Sci Adv. 2018;4:eaao5838 pubmed publisher |
- western blot; human; loading ...; fig 10j
| Panda S, Facchinetti V, Voynova E, Hanabuchi S, Karnell J, Hanna R, et al. Galectin-9 inhibits TLR7-mediated autoimmunity in murine lupus models. J Clin Invest. 2018;128:1873-1887 pubmed publisher |
- immunocytochemistry; mouse; 1:100; loading ...; fig s5d
| Hu J, Sun F, Handel M. Nuclear localization of EIF4G3 suggests a role for the XY body in translational regulation during spermatogenesis in mice. Biol Reprod. 2018;98:102-114 pubmed publisher |
- western blot; human; 1:1000; loading ...; fig s2a
| Mai W, Gosa L, Daniëls V, Ta L, Tsang J, Higgins B, et al. Cytoplasmic p53 couples oncogene-driven glucose metabolism to apoptosis and is a therapeutic target in glioblastoma. Nat Med. 2017;23:1342-1351 pubmed publisher |
- western blot; human; loading ...; fig s4e
| Gupta A, Anjomani Virmouni S, Koundouros N, Dimitriadi M, Choo Wing R, Valle A, et al. PARK2 Depletion Connects Energy and Oxidative Stress to PI3K/Akt Activation via PTEN S-Nitrosylation. Mol Cell. 2017;65:999-1013.e7 pubmed publisher |
- western blot; human; loading ...; fig 2a
| Wolfson R, Chantranupong L, Wyant G, Gu X, Orozco J, Shen K, et al. KICSTOR recruits GATOR1 to the lysosome and is necessary for nutrients to regulate mTORC1. Nature. 2017;543:438-442 pubmed publisher |
- western blot; human; loading ...; fig s7f
| Jung J, Nayak A, Schaeffer V, Starzetz T, Kirsch A, Muller S, et al. Multiplex image-based autophagy RNAi screening identifies SMCR8 as ULK1 kinase activity and gene expression regulator. elife. 2017;6: pubmed publisher |
- western blot; mouse; loading ...; fig 7a
| Qiao A, Jin X, Pang J, Moskophidis D, Mivechi N. The transcriptional regulator of the chaperone response HSF1 controls hepatic bioenergetics and protein homeostasis. J Cell Biol. 2017;216:723-741 pubmed publisher |
- western blot; pigs ; loading ...; fig 2a
| Ren W, Yin J, Xiao H, Chen S, Liu G, Tan B, et al. Intestinal Microbiota-Derived GABA Mediates Interleukin-17 Expression during Enterotoxigenic Escherichia coli Infection. Front Immunol. 2016;7:685 pubmed publisher |
- western blot; mouse; loading ...; fig 6b
| Yamauchi T, Nishiyama M, Moroishi T, Kawamura A, Nakayama K. FBXL5 Inactivation in Mouse Brain Induces Aberrant Proliferation of Neural Stem Progenitor Cells. Mol Cell Biol. 2017;37: pubmed publisher |
- western blot; human; loading ...; fig s3
| Reuther C, Heinzle V, Nölting S, Herterich S, Hahner S, Halilovic E, et al. The HDM2 (MDM2) Inhibitor NVP-CGM097 Inhibits Tumor Cell Proliferation and Shows Additive Effects with 5-Fluorouracil on the p53-p21-Rb-E2F1 Cascade in the p53wild type Neuroendocrine Tumor Cell Line GOT1. Neuroendocrinology. 2018;106:1-19 pubmed publisher |
- western blot; human; 1:1000; loading ...; fig 3a
| Li R, Xu J, Fu C, Zhang J, Zheng Y, Jia H, et al. Regulation of mTORC1 by lysosomal calcium and calmodulin. elife. 2016;5: pubmed publisher |
- western blot; human; 1:1000; loading ...; fig 3c
| Chen Y, Xu J, Skanderup A, Dong Y, Brannon A, Wang L, et al. Molecular analysis of aggressive renal cell carcinoma with unclassified histology reveals distinct subsets. Nat Commun. 2016;7:13131 pubmed publisher |
- western blot; human; fig 3
| Yuzugullu H, Von T, Thorpe L, Walker S, Roberts T, Frank D, et al. NTRK2 activation cooperates with PTEN deficiency in T-ALL through activation of both the PI3K-AKT and JAK-STAT3 pathways. Cell Discov. 2016;2:16030 pubmed publisher |
- western blot; human; 1:1000; fig st1
| Treindl F, Ruprecht B, Beiter Y, Schultz S, Döttinger A, Staebler A, et al. A bead-based western for high-throughput cellular signal transduction analyses. Nat Commun. 2016;7:12852 pubmed publisher |
- western blot; human; 1:1000; fig 2
| Carbonneau M, M Gagné L, Lalonde M, Germain M, Motorina A, Guiot M, et al. The oncometabolite 2-hydroxyglutarate activates the mTOR signalling pathway. Nat Commun. 2016;7:12700 pubmed publisher |
- western blot; mouse; loading ...; fig s3c
| Tsai S, Rodriguez A, Dastidar S, Del Greco E, Carr K, Sitzmann J, et al. Increased 4E-BP1 Expression Protects against Diet-Induced Obesity and Insulin Resistance in Male Mice. Cell Rep. 2016;16:1903-14 pubmed publisher |
- western blot; mouse; 1:2000; fig 6
| Jiang M, Liu L, He X, Wang H, Lin W, Wang H, et al. Regulation of PERK-eIF2? signalling by tuberous sclerosis complex-1 controls homoeostasis and survival of myelinating oligodendrocytes. Nat Commun. 2016;7:12185 pubmed publisher |
- western blot; mouse; 1:1000; fig 7
| Velázquez R, Shaw D, Caccamo A, Oddo S. Pim1 inhibition as a novel therapeutic strategy for Alzheimer's disease. Mol Neurodegener. 2016;11:52 pubmed publisher |
- western blot; mouse; 1:1000; fig s3
| Wang J, Farris A, Xu K, Wang P, Zhang X, Duong D, et al. GPRC5A suppresses protein synthesis at the endoplasmic reticulum to prevent radiation-induced lung tumorigenesis. Nat Commun. 2016;7:11795 pubmed publisher |
- western blot; mouse; fig s1
| van Ree J, Nam H, Jeganathan K, Kanakkanthara A, van Deursen J. Pten regulates spindle pole movement through Dlg1-mediated recruitment of Eg5 to centrosomes. Nat Cell Biol. 2016;18:814-21 pubmed publisher |
| Zhu Y, Ramos da Silva S, He M, Liang Q, Lu C, Feng P, et al. An Oncogenic Virus Promotes Cell Survival and Cellular Transformation by Suppressing Glycolysis. PLoS Pathog. 2016;12:e1005648 pubmed publisher |
| Humoud M, Doyle N, Royall E, Willcocks M, Sorgeloos F, van Kuppeveld F, et al. Feline Calicivirus Infection Disrupts Assembly of Cytoplasmic Stress Granules and Induces G3BP1 Cleavage. J Virol. 2016;90:6489-6501 pubmed publisher |
- western blot; human; loading ...; fig 5a
| Xu K, Wang L, Feng W, Feng Y, Shu H. Phosphatidylinositol-3 kinase-dependent translational regulation of Id1 involves the PPM1G phosphatase. Oncogene. 2016;35:5807-5816 pubmed publisher |
- western blot; human; fig 6
| Strickland S, Vande Pol S. The Human Papillomavirus 16 E7 Oncoprotein Attenuates AKT Signaling To Promote Internal Ribosome Entry Site-Dependent Translation and Expression of c-MYC. J Virol. 2016;90:5611-5621 pubmed publisher |
- western blot; mouse; loading ...; fig 7a
| Lakshmipathi J, Alvarez Perez J, Rosselot C, Casinelli G, Stamateris R, Rausell Palamos F, et al. PKCζ Is Essential for Pancreatic β-Cell Replication During Insulin Resistance by Regulating mTOR and Cyclin-D2. Diabetes. 2016;65:1283-96 pubmed publisher |
- western blot; mouse; 1:1000; fig 3i
| Nakazawa M, Eisinger Mathason T, Sadri N, Ochocki J, Gade T, Amin R, et al. Epigenetic re-expression of HIF-2α suppresses soft tissue sarcoma growth. Nat Commun. 2016;7:10539 pubmed publisher |
- western blot; mouse; fig 4
| Hukelmann J, Anderson K, Sinclair L, Grzes K, Murillo A, Hawkins P, et al. The cytotoxic T cell proteome and its shaping by the kinase mTOR. Nat Immunol. 2016;17:104-12 pubmed publisher |
- western blot; human; loading ...; fig 4b
| Reuther C, Heinzle V, Spampatti M, Vlotides G, de Toni E, Spöttl G, et al. Cabozantinib and Tivantinib, but Not INC280, Induce Antiproliferative and Antimigratory Effects in Human Neuroendocrine Tumor Cells in vitro: Evidence for 'Off-Target' Effects Not Mediated by c-Met Inhibition. Neuroendocrinology. 2016;103:383-401 pubmed publisher |
| Jansova D, Koncicka M, Tetkova A, Cerna R, Malik R, Del Llano E, et al. Regulation of 4E-BP1 activity in the mammalian oocyte. Cell Cycle. 2017;16:927-939 pubmed publisher |
| Mateo F, Arenas E, Aguilar H, Serra Musach J, de Garibay G, Boni J, et al. Stem cell-like transcriptional reprogramming mediates metastatic resistance to mTOR inhibition. Oncogene. 2017;36:2737-2749 pubmed publisher |
| Henry W, Laszewski T, Tsang T, Beca F, Beck A, McAllister S, et al. Aspirin Suppresses Growth in PI3K-Mutant Breast Cancer by Activating AMPK and Inhibiting mTORC1 Signaling. Cancer Res. 2017;77:790-801 pubmed publisher |
| Xie C, Wu X, Long C, Wang Q, Fan Z, Li S, et al. Chitosan oligosaccharide affects antioxidant defense capacity and placental amino acids transport of sows. BMC Vet Res. 2016;12:243 pubmed |
| Hodgson L, Spiering D, Sabouri Ghomi M, Dagliyan O, DerMardirossian C, Danuser G, et al. FRET binding antenna reports spatiotemporal dynamics of GDI-Cdc42 GTPase interactions. Nat Chem Biol. 2016;12:802-809 pubmed publisher |
| Blas Rus N, Bustos Morán E, Perez de Castro I, de Carcer G, Borroto A, Camafeita E, et al. Aurora A drives early signalling and vesicle dynamics during T-cell activation. Nat Commun. 2016;7:11389 pubmed publisher |
