product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
Phospho-Tyrosine Mouse mAb (P-Tyr-100)
catalog :
9411
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
antigen modification :
phosphorylated
clone name :
Phospho-Tyrosine Mouse
reactivity :
human, mouse
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, other
citations: 61
Published Application/Species/Sample/DilutionReference
  • western blot; human; loading ...; fig 3f
Hu F, Song D, Yan Y, Huang C, Shen C, Lan J, et al. IL-6 regulates autophagy and chemotherapy resistance by promoting BECN1 phosphorylation. Nat Commun. 2021;12:3651 pubmed publisher
  • western blot; human; loading ...; fig s5c
Zhou J, Cui S, He Q, Guo Y, Pan X, Zhang P, et al. SUMOylation inhibitors synergize with FXR agonists in combating liver fibrosis. Nat Commun. 2020;11:240 pubmed publisher
  • western blot; human; loading ...; fig 6e
Gao X, Zhao L, Liu S, Li Y, Xia S, Chen D, et al. γ-6-Phosphogluconolactone, a Byproduct of the Oxidative Pentose Phosphate Pathway, Contributes to AMPK Activation through Inhibition of PP2A. Mol Cell. 2019;76:857-871.e9 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 6e
Zhang H, Sathyamurthy A, Liu F, Li L, Zhang L, Dong Z, et al. Agrin-Lrp4-Ror2 signaling regulates adult hippocampal neurogenesis in mice. elife. 2019;8: pubmed publisher
  • western blot; human; loading ...; fig s3a
Kennedy S, Han J, Portman N, Nobis M, Hastings J, Murphy K, et al. Targeting promiscuous heterodimerization overcomes innate resistance to ERBB2 dimerization inhibitors in breast cancer. Breast Cancer Res. 2019;21:43 pubmed publisher
  • western blot; human; loading ...; fig 5b
Hou L, Zhao J, Gao S, Ji T, Song T, Li Y, et al. Restriction of hepatitis B virus replication by c-Abl-induced proteasomal degradation of the viral polymerase. Sci Adv. 2019;5:eaau7130 pubmed publisher
  • immunohistochemistry - paraffin section; human
  • immunohistochemistry; human
Lin J, Izar B, Wang S, Yapp C, Mei S, Shah P, et al. Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes. elife. 2018;7: pubmed publisher
  • immunocytochemistry; human; 1:100; loading ...; fig 2e
  • western blot; human; loading ...; fig 1c
  • immunocytochemistry; mouse; 1:100; loading ...; fig 3c
Carpenter V, Chen Y, Dolat L, Valdivia R. The Effector TepP Mediates Recruitment and Activation of Phosphoinositide 3-Kinase on Early Chlamydia trachomatis Vacuoles. mSphere. 2017;2: pubmed publisher
  • immunoprecipitation; mouse; fig 2a
Matsumoto Y, La Rose J, Kent O, Wagner M, Narimatsu M, Levy A, et al. Reciprocal stabilization of ABL and TAZ regulates osteoblastogenesis through transcription factor RUNX2. J Clin Invest. 2016;126:4482-4496 pubmed publisher
  • western blot; mouse; 1:2000; fig 6
Sun X, Li L, Liu F, Huang Z, Bean J, Jiao H, et al. Lrp4 in astrocytes modulates glutamatergic transmission. Nat Neurosci. 2016;19:1010-8 pubmed publisher
  • western blot; human; loading ...; fig s7a
Francavilla C, Papetti M, Rigbolt K, Pedersen A, Sigurdsson J, Cazzamali G, et al. Multilayered proteomics reveals molecular switches dictating ligand-dependent EGFR trafficking. Nat Struct Mol Biol. 2016;23:608-18 pubmed publisher
  • immunocytochemistry; human; 1:400; fig 6
Horton E, Humphries J, Stutchbury B, Jacquemet G, Ballestrem C, Barry S, et al. Modulation of FAK and Src adhesion signaling occurs independently of adhesion complex composition. J Cell Biol. 2016;212:349-64 pubmed publisher
  • western blot; human; fig 6
Díaz Barreiro A, Bernal Quirós M, Georg I, Marañón C, Alarcón Riquelme M, Castillejo López C. The SLE variant Ala71Thr of BLK severely decreases protein abundance and binding to BANK1 through impairment of the SH3 domain function. Genes Immun. 2016;17:128-38 pubmed publisher
  • western blot; human; 1:1000
Lee S, Frattini V, Bansal M, Castano A, Sherman D, Hutchinson K, et al. An ID2-dependent mechanism for VHL inactivation in cancer. Nature. 2016;529:172-7 pubmed publisher
  • immunoprecipitation; human; fig 1c
Wu X, Zahari M, Renuse S, Nirujogi R, Kim M, Manda S, et al. Phosphoproteomic Analysis Identifies Focal Adhesion Kinase 2 (FAK2) as a Potential Therapeutic Target for Tamoxifen Resistance in Breast Cancer. Mol Cell Proteomics. 2015;14:2887-900 pubmed publisher
  • other; mouse; 1:2000; fig s1
Chiang C, Uzoma I, Lane D, Memišević V, Alem F, Yao K, et al. A reverse-phase protein microarray-based screen identifies host signaling dynamics upon Burkholderia spp. infection. Front Microbiol. 2015;6:683 pubmed publisher
  • western blot; human; 1:2000; fig 3
Bugaj L, Spelke D, Mesuda C, Varedi M, Kane R, Schaffer D. Regulation of endogenous transmembrane receptors through optogenetic Cry2 clustering. Nat Commun. 2015;6:6898 pubmed publisher
  • western blot; mouse; fig 2
Naik E, Webster J, DeVoss J, Liu J, Suriben R, Dixit V. Regulation of proximal T cell receptor signaling and tolerance induction by deubiquitinase Usp9X. J Exp Med. 2014;211:1947-55 pubmed publisher
  • immunoprecipitation; mouse; 1:100; fig 4
Moorwood C, Philippou A, Spinazzola J, Keyser B, Macarak E, Barton E. Absence of ?-sarcoglycan alters the response of p70S6 kinase to mechanical perturbation in murine skeletal muscle. Skelet Muscle. 2014;4:13 pubmed publisher
Orfi Z, Wáczek F, Baska F, Szabadkai I, Torka R, Hartmann J, et al. Novel members of quinoline compound family enhance insulin secretion in RIN-5AH beta cells and in rat pancreatic islet microtissue. Sci Rep. 2017;7:44073 pubmed publisher
Gao S, Geng C, Song T, Lin X, Liu J, Cai Z, et al. Activation of c-Abl Kinase Potentiates the Anti-myeloma Drug Lenalidomide by Promoting DDA1 Protein Recruitment to the CRL4 Ubiquitin Ligase. J Biol Chem. 2017;292:3683-3691 pubmed publisher
Zhang J, Wang S, Jiang B, Huang L, Ji Z, Li X, et al. c-Src phosphorylation and activation of hexokinase promotes tumorigenesis and metastasis. Nat Commun. 2017;8:13732 pubmed publisher
Lin C, Lin W, Cho R, Wang C, Hsiao L, Yang C. TNF-?-Induced cPLA2 Expression via NADPH Oxidase/Reactive Oxygen Species-Dependent NF-?B Cascade on Human Pulmonary Alveolar Epithelial Cells. Front Pharmacol. 2016;7:447 pubmed
Cheng H, Burroughs Garcia J, Birkness J, Trinidad J, Deans M. Disparate Regulatory Mechanisms Control Fat3 and P75NTR Protein Transport through a Conserved Kif5-Interaction Domain. PLoS ONE. 2016;11:e0165519 pubmed publisher
Theriault P, ElAli A, Rivest S. High fat diet exacerbates Alzheimer's disease-related pathology in APPswe/PS1 mice. Oncotarget. 2016;7:67808-67827 pubmed publisher
Justa Schuch D, Silva Garcia M, Pilla E, Engelke M, Kilisch M, Lenz C, et al. DPP9 is a novel component of the N-end rule pathway targeting the tyrosine kinase Syk. elife. 2016;5: pubmed publisher
Jadwin J, Oh D, Curran T, Ogiue Ikeda M, Jia L, White F, et al. Time-resolved multimodal analysis of Src Homology 2 (SH2) domain binding in signaling by receptor tyrosine kinases. elife. 2016;5:e11835 pubmed publisher
Altuntas S, Rossin F, Marsella C, D Eletto M, Diaz Hidalgo L, Farrace M, et al. The transglutaminase type 2 and pyruvate kinase isoenzyme M2 interplay in autophagy regulation. Oncotarget. 2015;6:44941-54 pubmed publisher
Friedman A, Amzallag A, Pruteanu Malinici I, Baniya S, Cooper Z, Piris A, et al. Landscape of Targeted Anti-Cancer Drug Synergies in Melanoma Identifies a Novel BRAF-VEGFR/PDGFR Combination Treatment. PLoS ONE. 2015;10:e0140310 pubmed publisher
Cao X, Kaneko T, Li J, Liu A, Voss C, Li S. A phosphorylation switch controls the spatiotemporal activation of Rho GTPases in directional cell migration. Nat Commun. 2015;6:7721 pubmed publisher
Koos B, Cane G, Grannas K, Löf L, Arngården L, Heldin J, et al. Proximity-dependent initiation of hybridization chain reaction. Nat Commun. 2015;6:7294 pubmed publisher
Tampella G, Kerns H, Niu D, Singh S, Khim S, Bosch K, et al. The Tec Kinase-Regulated Phosphoproteome Reveals a Mechanism for the Regulation of Inhibitory Signals in Murine Macrophages. J Immunol. 2015;195:246-56 pubmed publisher
Lou S, Diz Muñoz A, Weiner O, Fletcher D, Theriot J. Myosin light chain kinase regulates cell polarization independently of membrane tension or Rho kinase. J Cell Biol. 2015;209:275-88 pubmed publisher
Iorio V, Festa M, Rosati A, Hahne M, Tiberti C, Capunzo M, et al. BAG3 regulates formation of the SNARE complex and insulin secretion. Cell Death Dis. 2015;6:e1684 pubmed publisher
Mills R, Mita M, Nakagawa J, Shoji M, Sutherland C, Walsh M. A role for the tyrosine kinase Pyk2 in depolarization-induced contraction of vascular smooth muscle. J Biol Chem. 2015;290:8677-92 pubmed publisher
Tulake K, Wang X, Chen Y, Yu C, Jing B, Li H. Protein tyrosine phosphorylation during capacitation in sperm of a rare red deer, Tarim wapiti (Cervus elaphus yarkandensis). Anim Reprod Sci. 2015;154:68-78 pubmed publisher
Suraneni P, Fogelson B, Rubinstein B, Noguera P, Volkmann N, Hanein D, et al. A mechanism of leading-edge protrusion in the absence of Arp2/3 complex. Mol Biol Cell. 2015;26:901-12 pubmed publisher
Wiens O, Xia D, von Schubert C, Wastling J, Dobbelaere D, Heussler V, et al. Cell cycle-dependent phosphorylation of Theileria annulata schizont surface proteins. PLoS ONE. 2014;9:e103821 pubmed publisher
Lind J, Backert S, Pfleiderer K, Berg D, Yamaoka Y, Sticht H, et al. Systematic analysis of phosphotyrosine antibodies recognizing single phosphorylated EPIYA-motifs in CagA of Western-type Helicobacter pylori strains. PLoS ONE. 2014;9:e96488 pubmed publisher
Guilgur L, Prudêncio P, Sobral D, Liszeková D, Rosa A, Martinho R. Requirement for highly efficient pre-mRNA splicing during Drosophila early embryonic development. elife. 2014;3:e02181 pubmed publisher
Tobin G, Zhang J, Goodwin D, Stewart S, Xu L, Knapton A, et al. The role of eNOS phosphorylation in causing drug-induced vascular injury. Toxicol Pathol. 2014;42:709-24 pubmed publisher
Chesarino N, McMichael T, Hach J, Yount J. Phosphorylation of the antiviral protein interferon-inducible transmembrane protein 3 (IFITM3) dually regulates its endocytosis and ubiquitination. J Biol Chem. 2014;289:11986-92 pubmed publisher
Chen Y, Bastidas R, Saka H, Carpenter V, Richards K, Plano G, et al. The Chlamydia trachomatis type III secretion chaperone Slc1 engages multiple early effectors, including TepP, a tyrosine-phosphorylated protein required for the recruitment of CrkI-II to nascent inclusions and innate immune signaling. PLoS Pathog. 2014;10:e1003954 pubmed publisher
Ma M, Baumgartner M. Filopodia and membrane blebs drive efficient matrix invasion of macrophages transformed by the intracellular parasite Theileria annulata. PLoS ONE. 2013;8:e75577 pubmed publisher
Sima N, Cheng X, Ye F, Ma D, Xie X, Lu W. The overexpression of scaffolding protein NEDD9 promotes migration and invasion in cervical cancer via tyrosine phosphorylated FAK and SRC. PLoS ONE. 2013;8:e74594 pubmed publisher
Wiradjaja F, Cottle D, Jones L, Smyth I. Regulation of PDGFC signalling and extracellular matrix composition by FREM1 in mice. Dis Model Mech. 2013;6:1426-33 pubmed publisher
Saldana S, Lee H, Lowery F, Khotskaya Y, Xia W, Zhang C, et al. Inhibition of type I insulin-like growth factor receptor signaling attenuates the development of breast cancer brain metastasis. PLoS ONE. 2013;8:e73406 pubmed publisher
Pease B, Huttlin E, Jedrychowski M, Talevich E, Harmon J, Dillman T, et al. Global analysis of protein expression and phosphorylation of three stages of Plasmodium falciparum intraerythrocytic development. J Proteome Res. 2013;12:4028-45 pubmed publisher
Taipale M, Krykbaeva I, Whitesell L, Santagata S, Zhang J, Liu Q, et al. Chaperones as thermodynamic sensors of drug-target interactions reveal kinase inhibitor specificities in living cells. Nat Biotechnol. 2013;31:630-7 pubmed publisher
Sigurdsson V, Ingthorsson S, Hilmarsdottir B, Gustafsdottir S, Franzdóttir S, Arason A, et al. Expression and functional role of sprouty-2 in breast morphogenesis. PLoS ONE. 2013;8:e60798 pubmed publisher
Bernal Quirós M, Wu Y, Alarcon Riquelme M, Castillejo Lopez C. BANK1 and BLK act through phospholipase C gamma 2 in B-cell signaling. PLoS ONE. 2013;8:e59842 pubmed publisher
Slanina H, Hebling S, Hauck C, Schubert Unkmeir A. Cell invasion by Neisseria meningitidis requires a functional interplay between the focal adhesion kinase, Src and cortactin. PLoS ONE. 2012;7:e39613 pubmed publisher
Ji L, Jayachandran G, Roth J. High throughput profiling of serum phosphoproteins/peptides using the SELDI-TOF-MS platform. Methods Mol Biol. 2012;818:199-216 pubmed publisher
Chong T, Stary J, Wang Y, Newmark P. Molecular markers to characterize the hermaphroditic reproductive system of the planarian Schmidtea mediterranea. BMC Dev Biol. 2011;11:69 pubmed publisher
Hereng T, Elgstøen K, Cederkvist F, Eide L, Jahnsen T, Skalhegg B, et al. Exogenous pyruvate accelerates glycolysis and promotes capacitation in human spermatozoa. Hum Reprod. 2011;26:3249-63 pubmed publisher
Weibrecht I, Grundberg I, Nilsson M, Söderberg O. Simultaneous visualization of both signaling cascade activity and end-point gene expression in single cells. PLoS ONE. 2011;6:e20148 pubmed publisher
Khusial P, Vadla B, Krishnan H, Ramlall T, Shen Y, Ichikawa H, et al. Src activates Abl to augment Robo1 expression in order to promote tumor cell migration. Oncotarget. 2010;1:198-209 pubmed
Rubert J, Qian Z, Andraos R, Guthy D, Radimerski T. Bim and Mcl-1 exert key roles in regulating JAK2V617F cell survival. BMC Cancer. 2011;11:24 pubmed publisher
Jackman J, Chen Y, Huang A, Moffat B, Scheer J, Leong S, et al. Development of a two-part strategy to identify a therapeutic human bispecific antibody that inhibits IgE receptor signaling. J Biol Chem. 2010;285:20850-9 pubmed publisher
Ye Z, Kluger Y, Lian Z, Weissman S. Two types of precursor cells in a multipotential hematopoietic cell line. Proc Natl Acad Sci U S A. 2005;102:18461-6 pubmed
Smith J, Poteet Smith C, Xu Y, Errington T, Hecht S, Lannigan D. Identification of the first specific inhibitor of p90 ribosomal S6 kinase (RSK) reveals an unexpected role for RSK in cancer cell proliferation. Cancer Res. 2005;65:1027-34 pubmed
product information
SKU :
9411S
Product-Name :
Phospho-Tyrosine Mouse mAb (P-Tyr-100)
Size :
300 ug
Price-(USD) :
199 USD
Species-x-Reactivity :
All
Applications :
ELISA Peptide
Product-Category :
Motif Antibodies
Shipping-Temp :
COLD
Storage-Temp :
-20°C
Product-Type :
Monoclonal Antibody
Host :
Mouse
Target :
Phospho-Tyrosine
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
https://www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.