| Published Application/Species/Dilution | Reference |
|---|
- western blot (knockout validation); human; fig 5
| Wu C, Huang K, Yang T, Li Y, Wen C, Hsu S, et al. The Topoisomerase 1 Inhibitor Austrobailignan-1 Isolated from Koelreuteria henryi Induces a G2/M-Phase Arrest and Cell Death Independently of p53 in Non-Small Cell Lung Cancer Cells. PLoS ONE. 2015;10:e0132052 pubmed publisher |
- western blot (knockout validation); mouse; fig 4a
| Kawabata T, Yamaguchi S, Buske T, Luebben S, Wallace M, Matise I, et al. A reduction of licensed origins reveals strain-specific replication dynamics in mice. Mamm Genome. 2011;22:506-17 pubmed publisher |
- western blot; human; fig 1
| Pandiri I, Chen Y, Joe Y, Kim H, Park J, Chung H, et al. Tristetraprolin mediates the anti-proliferative effects of metformin in breast cancer cells. Breast Cancer Res Treat. 2016;156:57-64 pubmed publisher |
- western blot; human; fig 6
| Chiang T, le Sage C, Larrieu D, Demir M, Jackson S. CRISPR-Cas9(D10A) nickase-based genotypic and phenotypic screening to enhance genome editing. Sci Rep. 2016;6:24356 pubmed publisher |
- western blot; mouse; 1:1000; fig 5
| Min J, Guo K, Suryadevara P, Zhu F, Holbrook G, Chen Y, et al. Optimization of a Novel Series of Ataxia-Telangiectasia Mutated Kinase Inhibitors as Potential Radiosensitizing Agents. J Med Chem. 2016;59:559-77 pubmed publisher |
- western blot; human; fig 5j
| Capell B, Drake A, Zhu J, Shah P, Dou Z, Dorsey J, et al. MLL1 is essential for the senescence-associated secretory phenotype. Genes Dev. 2016;30:321-36 pubmed publisher |
- western blot; mouse; 1:1000; fig 6
| Koppaka V, Chen Y, Mehta G, Orlicky D, Thompson D, Jester J, et al. ALDH3A1 Plays a Functional Role in Maintenance of Corneal Epithelial Homeostasis. PLoS ONE. 2016;11:e0146433 pubmed publisher |
- western blot; human; fig 3a
| Petroni M, Sardina F, Heil C, Sahún-Roncero M, Colicchia V, Veschi V, et al. The MRN complex is transcriptionally regulated by MYCN during neural cell proliferation to control replication stress. Cell Death Differ. 2016;23:197-206 pubmed publisher |
- western blot; human; fig 1b
| Kemp M, Sancar A. ATR Kinase Inhibition Protects Non-cycling Cells from the Lethal Effects of DNA Damage and Transcription Stress. J Biol Chem. 2016;: pubmed |
| Mortusewicz O, Evers B, Helleday T. PC4 promotes genome stability and DNA repair through binding of ssDNA at DNA damage sites. Oncogene. 2016;35:761-70 pubmed publisher |
- western blot; mouse; fig 3
| He D, Xiang J, Li B, Liu H. The dynamic behavior of Ect2 in response to DNA damage. Sci Rep. 2016;6:24504 pubmed publisher |
- western blot; human; 1:1000; fig 1
| Ortega-Atienza S, Wong V, Deloughery Z, Luczak M, Zhitkovich A. ATM and KAT5 safeguard replicating chromatin against formaldehyde damage. Nucleic Acids Res. 2016;44:198-209 pubmed publisher |
- western blot; mouse; fig s1
| Barroso-González J, Auclair S, Luan S, Thomas L, Atkins K, Aslan J, et al. PACS-2 mediates the ATM and NF-κB-dependent induction of anti-apoptotic Bcl-xL in response to DNA damage. Cell Death Differ. 2016;: pubmed publisher |
- western blot; human; fig s5
| Cristini A, Park J, Capranico G, Legube G, Favre G, Sordet O. DNA-PK triggers histone ubiquitination and signaling in response to DNA double-strand breaks produced during the repair of transcription-blocking topoisomerase I lesions. Nucleic Acids Res. 2016;44:1161-78 pubmed publisher |
- western blot; human; fig 1
| Pecháčková S, Burdova K, Benada J, Kleiblova P, Jenikova G, Macurek L. Inhibition of WIP1 phosphatase sensitizes breast cancer cells to genotoxic stress and to MDM2 antagonist nutlin-3. Oncotarget. 2016;7:14458-75 pubmed publisher |
- western blot; human; fig 2
| Kazantseva J, Sadam H, Neuman T, Palm K. Targeted alternative splicing of TAF4: a new strategy for cell reprogramming. Sci Rep. 2016;6:30852 pubmed publisher |
- western blot; human; 1:1000; fig 5
| Lee W, Jo S, Lee M, Won C, Lee M, Choi J, et al. The Effect of MCP-1/CCR2 on the Proliferation and Senescence of Epidermal Constituent Cells in Solar Lentigo. Int J Mol Sci. 2016;17: pubmed publisher |
| Delmas E, Jah N, Pirou C, Bouleau S, Le Floch N, Vayssiere J, et al. FGF1 C-terminal domain and phosphorylation regulate intracrine FGF1 signaling for its neurotrophic and anti-apoptotic activities. Cell Death Dis. 2016;7:e2079 pubmed publisher |
- western blot; human; fig 1
| Hall A, Lu W, Godfrey J, Antonov A, Paicu C, Moxon S, et al. The cytoskeleton adaptor protein ankyrin-1 is upregulated by p53 following DNA damage and alters cell migration. Cell Death Dis. 2016;7:e2184 pubmed publisher |
- western blot; human; 1:1000; fig 4
| Ohashi A, Ohori M, Iwai K, Nakayama Y, Nambu T, Morishita D, et al. Aneuploidy generates proteotoxic stress and DNA damage concurrently with p53-mediated post-mitotic apoptosis in SAC-impaired cells. Nat Commun. 2015;6:7668 pubmed publisher |
- western blot; human; 1:1000; fig 3
| Cañeque T, Gomes F, Mai T, Maestri G, Malacria M, Rodriguez R. Synthesis of marmycin A and investigation into its cellular activity. Nat Chem. 2015;7:744-51 pubmed publisher |
- other; mouse; 1:1000; fig s1
| Chiang C, Uzoma I, Lane D, Memisevic V, Alem F, Yao K, et al. A reverse-phase protein microarray-based screen identifies host signaling dynamics upon Burkholderia spp. infection. Front Microbiol. 2015;6:683 pubmed publisher |
- western blot; human; 1:500; fig 2.e
| Laberge R, Sun Y, Orjalo A, Patil C, Freund A, Zhou L, et al. MTOR regulates the pro-tumorigenic senescence-associated secretory phenotype by promoting IL1A translation. Nat Cell Biol. 2015;17:1049-61 pubmed publisher |
- western blot; human; 1:1000
| Ortega-Atienza S, Green S, Zhitkovich A. Proteasome activity is important for replication recovery, CHK1 phosphorylation and prevention of G2 arrest after low-dose formaldehyde. Toxicol Appl Pharmacol. 2015;286:135-41 pubmed publisher |
- western blot; human; fig 6d
| Hilton B, Li Z, Musich P, Wang H, Cartwright B, SERRANO M, et al. ATR Plays a Direct Antiapoptotic Role at Mitochondria, which Is Regulated by Prolyl Isomerase Pin1. Mol Cell. 2015;60:35-46 pubmed publisher |
| Kiyonari S, Iimori M, Matsuoka K, Watanabe S, Morikawa-Ichinose T, Miura D, et al. The 1,2-Diaminocyclohexane Carrier Ligand in Oxaliplatin Induces p53-Dependent Transcriptional Repression of Factors Involved in Thymidylate Biosynthesis. Mol Cancer Ther. 2015;14:2332-42 pubmed publisher |
- western blot; rabbit; 1:1000; fig 3
| Varela A, Piperi C, Sigala F, Agrogiannis G, Davos C, Andri M, et al. Elevated expression of mechanosensory polycystins in human carotid atherosclerotic plaques: association with p53 activation and disease severity. Sci Rep. 2015;5:13461 pubmed publisher |
- western blot; mouse; 1:1000; fig 3
| Zhao J, Li H, Zhou R, Ma G, Dekker J, Tucker H, et al. Foxp1 Regulates the Proliferation of Hair Follicle Stem Cells in Response to Oxidative Stress during Hair Cycling. PLoS ONE. 2015;10:e0131674 pubmed publisher |
- western blot; human; 1:500
| Tréhoux S, Lahdaoui F, Delpu Y, Renaud F, Leteurtre E, Torrisani J, et al. Micro-RNAs miR-29a and miR-330-5p function as tumor suppressors by targeting the MUC1 mucin in pancreatic cancer cells. Biochim Biophys Acta. 2015;1853:2392-403 pubmed publisher |
| Simões M, Martins C, Ferreira F. Early intranuclear replication of African swine fever virus genome modifies the landscape of the host cell nucleus. Virus Res. 2015;210:1-7 pubmed publisher |
- western blot; human
- western blot; mouse
| Vassilopoulos A, Tominaga Y, Kim H, Lahusen T, Li B, Yu H, et al. WEE1 murine deficiency induces hyper-activation of APC/C and results in genomic instability and carcinogenesis. Oncogene. 2015;34:3023-35 pubmed publisher |
- western blot; human; 1:1000; fig 2
| Sedic M, Skibinski A, Brown N, Gallardo M, Mulligan P, Martinez P, et al. Haploinsufficiency for BRCA1 leads to cell-type-specific genomic instability and premature senescence. Nat Commun. 2015;6:7505 pubmed publisher |
| Xu H, Zhou Y, Coughlan K, Ding Y, Wang S, Wu Y, et al. AMPKα1 deficiency promotes cellular proliferation and DNA damage via p21 reduction in mouse embryonic fibroblasts. Biochim Biophys Acta. 2015;1853:65-73 pubmed publisher |
- immunohistochemistry - paraffin section; mouse; 1:200; tbl he following antibodies were used for IHC analysis: MCM3 (generated in rabbits immunized with synthetic peptide N-CSQEDTEQKRKRRK-C conjugated to KLH; Sigma-Genosys, UK; serum was used at 1:500 dilution), Ki67 (Master Diagnostica, 0003110QD; 1:500), ??H2AX (Millipore, 05-636; 1:100), pS15-p53 (Cell Signaling, 9284; 1:200), Ter119 (BD, 550565; 1:50), Pax5 (Santa Cruz Biotechnology, sc-1974; 1:50), CD3 (Santa Cruz Biotechnology, sc-1127; 1:50) and FVIII (Dako, A0082; 1:200).
| Alvarez S, Diaz M, Flach J, Rodríguez-Acebes S, López-Contreras A, Martinez D, et al. Replication stress caused by low MCM expression limits fetal erythropoiesis and hematopoietic stem cell functionality. Nat Commun. 2015;6:8548 pubmed publisher |
- western blot; human; fig 1
| Meena J, Cerutti A, Beichler C, Morita Y, Bruhn C, Kumar M, et al. Telomerase abrogates aneuploidy-induced telomere replication stress, senescence and cell depletion. EMBO J. 2015;34:1371-84 pubmed publisher |
| Xiong J, Todorova D, Su N, Kim J, Lee P, Shen Z, et al. Stemness factor Sall4 is required for DNA damage response in embryonic stem cells. J Cell Biol. 2015;208:513-20 pubmed publisher |
- western blot; human; 1:1000
| Ambrosio S, Amente S, Napolitano G, Di Palo G, Lania L, Majello B. MYC impairs resolution of site-specific DNA double-strand breaks repair. Mutat Res. 2015;774:6-13 pubmed publisher |
- immunohistochemistry - paraffin section; mouse; fig 6b
| Lebrun-Julien F, Suter U. Combined HDAC1 and HDAC2 Depletion Promotes Retinal Ganglion Cell Survival After Injury Through Reduction of p53 Target Gene Expression. ASN Neuro. 2015;7: pubmed publisher |
| Ji X, Lu H, Zhou Q, Luo K. LARP7 suppresses P-TEFb activity to inhibit breast cancer progression and metastasis. elife. 2014;3:e02907 pubmed publisher |
| Giovannini C, Minguzzi M, Baglioni M, Fornari F, Giannone F, Ravaioli M, et al. Suppression of p53 by Notch3 is mediated by Cyclin G1 and sustained by MDM2 and miR-221 axis in hepatocellular carcinoma. Oncotarget. 2014;5:10607-20 pubmed |
| Tomicic M, Aasland D, Naumann S, Meise R, Barckhausen C, Kaina B, et al. Translesion polymerase η is upregulated by cancer therapeutics and confers anticancer drug resistance. Cancer Res. 2014;74:5585-96 pubmed publisher |
- western blot; human; fig 1
| Klaus C, Kaemmerer E, Reinartz A, Schneider U, Plum P, Jeon M, et al. TP53 status regulates ACSL5-induced expression of mitochondrial mortalin in enterocytes and colorectal adenocarcinomas. Cell Tissue Res. 2014;357:267-78 pubmed publisher |
- western blot; mouse; fig 5
| Lin H, Lin S, Chung Y, Vonderfecht S, Camden J, Flodby P, et al. Dynamic involvement of ATG5 in cellular stress responses. Cell Death Dis. 2014;5:e1478 pubmed publisher |
- immunohistochemistry - paraffin section; human
- western blot; human
| Wang C, Wang J, Liu Z, Ma X, Wang X, Jin H, et al. Ubiquitin-specific protease 2a stabilizes MDM4 and facilitates the p53-mediated intrinsic apoptotic pathway in glioblastoma. Carcinogenesis. 2014;35:1500-9 pubmed publisher |
- western blot; rat; 1:1000
| Borkham-Kamphorst E, Schaffrath C, Van De Leur E, Haas U, Tihaa L, Meurer S, et al. The anti-fibrotic effects of CCN1/CYR61 in primary portal myofibroblasts are mediated through induction of reactive oxygen species resulting in cellular senescence, apoptosis and attenuated TGF-β signaling. Biochim Biophys Acta. 2014;1843:902-14 pubmed publisher |
| Lin B, Gupta D, Heinen C. Human pluripotent stem cells have a novel mismatch repair-dependent damage response. J Biol Chem. 2014;289:24314-24 pubmed publisher |
- western blot; rat; 1:1000
| Ureshino R, Hsu Y, do Carmo L, Yokomizo C, Nantes I, Smaili S. Inhibition of cytoplasmic p53 differentially modulates Ca(2+) signaling and cellular viability in young and aged striata. Exp Gerontol. 2014;58:120-7 pubmed publisher |
| Kemp M, Gaddameedhi S, Choi J, Hu J, Sancar A. DNA repair synthesis and ligation affect the processing of excised oligonucleotides generated by human nucleotide excision repair. J Biol Chem. 2014;289:26574-83 pubmed publisher |
- western blot; mouse; 1:10000; fig 2b
| Lindsey-Boltz L, Kemp M, Reardon J, DEROCCO V, Iyer R, Modrich P, et al. Coupling of human DNA excision repair and the DNA damage checkpoint in a defined in vitro system. J Biol Chem. 2014;289:5074-82 pubmed publisher |
- western blot; mouse; fig 2d
| Adeyemi R, Pintel D. Parvovirus-induced depletion of cyclin B1 prevents mitotic entry of infected cells. PLoS Pathog. 2014;10:e1003891 pubmed publisher |
- immunohistochemistry - paraffin section; mouse; 1:200
| Maserati M, Dai X, Walentuk M, Mager J. Identification of four genes required for mammalian blastocyst formation. Zygote. 2014;22:331-9 pubmed publisher |
- western blot; human; 1:500
| Kyathanahalli C, Marks J, Nye K, Lao B, Albrecht E, Aberdeen G, et al. Cross-species withdrawal of MCL1 facilitates postpartum uterine involution in both the mouse and baboon. Endocrinology. 2013;154:4873-84 pubmed publisher |
| Chen J, Ozanne S. Deep senescent human fibroblasts show diminished DNA damage foci but retain checkpoint capacity to oxidative stress. FEBS Lett. 2006;580:6669-73 pubmed |
| Liu C, Chen C, Huang A, Li J. Subamolide A, a component isolated from Cinnamomum subavenium, induces apoptosis mediated by mitochondria-dependent, p53 and ERK1/2 pathways in human urothelial carcinoma cell line NTUB1. J Ethnopharmacol. 2011;137:503-11 pubmed publisher |
- western blot; mouse; 1:1000
| Pusapati R, Rounbehler R, Hong S, Powers J, Yan M, Kiguchi K, et al. ATM promotes apoptosis and suppresses tumorigenesis in response to Myc. Proc Natl Acad Sci U S A. 2006;103:1446-51 pubmed |
| Kim S, Lim J, Lee J, Choe S, Kim Y, Song S, et al. Activation of β-catenin by inhibitors of glycogen synthase kinase-3 ameliorates cisplatin-induced cytotoxicity and pro-inflammatory cytokine expression in HEI-OC1 cells. Toxicology. 2014;320:74-82 pubmed publisher |
| Raab M, Kappel S, Kramer A, Sanhaji M, Matthess Y, Kurunci-Csacsko E, et al. Toxicity modelling of Plk1-targeted therapies in genetically engineered mice and cultured primary mammalian cells. Nat Commun. 2011;2:395 pubmed publisher |
| Lim S, Hung A, Porter A. Focused PCR screen reveals p53 dependence of nitric oxide-induced apoptosis and up-regulation of maspin and plasminogen activator inhibitor-1 in tumor cells. Mol Cancer Res. 2009;7:55-66 pubmed publisher |
