| Published Application/Species/Dilution | Reference |
|---|
- western blot; human; 1:100; fig st1
| Treindl F, Ruprecht B, Beiter Y, Schultz S, Döttinger A, Staebler A, et al. A bead-based western for high-throughput cellular signal transduction analyses. Nat Commun. 2016;7:12852 pubmed publisher |
- western blot; human; fig 4
| Nakayama R, Zhang Y, Czaplinski J, Anatone A, Sicinska E, Fletcher J, et al. Preclinical activity of selinexor, an inhibitor of XPO1, in sarcoma. Oncotarget. 2016;7:16581-92 pubmed publisher |
- western blot; human; fig 1a
| Thijssen R, Ter Burg J, van Bochove G, de Rooij M, Kuil A, Jansen M, et al. The pan phosphoinositide 3-kinase/mammalian target of rapamycin inhibitor SAR245409 (voxtalisib/XL765) blocks survival, adhesion and proliferation of primary chronic lymphocytic leukemia cells. Leukemia. 2016;30:337-45 pubmed publisher |
- western blot; mouse; fig 3
| Diersch S, Wirth M, Schneeweis C, Jörs S, Geisler F, Siveke J, et al. Kras(G12D) induces EGFR-MYC cross signaling in murine primary pancreatic ductal epithelial cells. Oncogene. 2016;35:3880-6 pubmed publisher |
- western blot; human; fig 2
| Christensen B, Nellemann B, Jørgensen J, Pedersen S, Jessen N. Erythropoietin does not activate erythropoietin receptor signaling or lipolytic pathways in human subcutaneous white adipose tissue in vivo. Lipids Health Dis. 2016;15:160 pubmed publisher |
- western blot; human; fig 5
| Afsar T, Trembley J, Salomon C, Razak S, Khan M, Ahmed K. Growth inhibition and apoptosis in cancer cells induced by polyphenolic compounds of Acacia hydaspica: Involvement of multiple signal transduction pathways. Sci Rep. 2016;6:23077 pubmed publisher |
- western blot; human; 1:1000
| Gassen N, Hartmann J, Zannas A, Kretzschmar A, Zschocke J, Maccarrone G, et al. FKBP51 inhibits GSK3β and augments the effects of distinct psychotropic medications. Mol Psychiatry. 2016;21:277-89 pubmed publisher |
- western blot; mouse; fig 4
| Nwadozi E, Roudier E, Rullman E, Tharmalingam S, Liu H, Gustafsson T, et al. Endothelial FoxO proteins impair insulin sensitivity and restrain muscle angiogenesis in response to a high-fat diet. FASEB J. 2016;: pubmed |
- western blot; mouse; 1:1000; fig 3
| Kim K, Qiang L, Hayden M, Sparling D, Purcell N, Pajvani U. mTORC1-independent Raptor prevents hepatic steatosis by stabilizing PHLPP2. Nat Commun. 2016;7:10255 pubmed publisher |
| Kawamoto E, Koshinaka K, Yoshimura T, Masuda H, Kawanaka K. Immobilization rapidly induces muscle insulin resistance together with the activation of MAPKs (JNK and p38) and impairment of AS160 phosphorylation. Physiol Rep. 2016;4: pubmed publisher |
- western blot; human; fig 3
| Hansen N, Hjort L, Broholm C, Gillberg L, Schrölkamp M, Schultz H, et al. Metabolic and Transcriptional Changes in Cultured Muscle Stem Cells from Low Birth Weight Subjects. J Clin Endocrinol Metab. 2016;101:2254-64 pubmed publisher |
- western blot; human; fig 1
- western blot; mouse; fig 7
| Kimball S, Gordon B, Moyer J, Dennis M, Jefferson L. Leucine induced dephosphorylation of Sestrin2 promotes mTORC1 activation. Cell Signal. 2016;: pubmed publisher |
| Fan S, Numata Y, Numata M. Endosomal Na+/H+ exchanger NHE5 influences MET recycling and cell migration. Mol Biol Cell. 2016;27:702-15 pubmed publisher |
- western blot; rat; 1:1000; fig 3
| Xing Y, Sun W, Wang Y, Gao F, Ma H. Mutual inhibition of insulin signaling and PHLPP-1 determines cardioprotective efficiency of Akt in aged heart. Aging (Albany NY). 2016;: pubmed |
- western blot; mouse; fig 2
| Liu D, Bordicchia M, Zhang C, Fang H, Wei W, Li J, et al. Activation of mTORC1 is essential for β-adrenergic stimulation of adipose browning. J Clin Invest. 2016;126:1704-16 pubmed publisher |
- western blot; mouse; fig 1
| Pinel A, Rigaudière J, Laillet B, Pouyet C, Malpuech-Brugère C, Prip-Buus C, et al. N-3PUFA differentially modulate palmitate-induced lipotoxicity through alterations of its metabolism in C2C12 muscle cells. Biochim Biophys Acta. 2016;1861:12-20 pubmed publisher |
- western blot; human; 1:1000; fig 3
| Liu C, Lin S, Hsu H, Yang S, Lin C, Yang M, et al. Suspension survival mediated by PP2A-STAT3-Col XVII determines tumour initiation and metastasis in cancer stem cells. Nat Commun. 2016;7:11798 pubmed publisher |
- western blot; human; fig 5
| Jeong A, Han S, Lee S, Su Park J, Lu Y, Yu S, et al. Patient derived mutation W257G of PPP2R1A enhances cancer cell migration through SRC-JNK-c-Jun pathway. Sci Rep. 2016;6:27391 pubmed publisher |
- western blot; human; fig 3
| Zhang Q, Wan M, Shi J, Horita D, Miller L, Kute T, et al. Yin Yang 1 promotes mTORC2-mediated AKT phosphorylation. J Mol Cell Biol. 2016;: pubmed |
| Zhang L, Zhang S, Yao J, Lowery F, Zhang Q, Huang W, et al. Microenvironment-induced PTEN loss by exosomal microRNA primes brain metastasis outgrowth. Nature. 2015;527:100-4 pubmed publisher |
- western blot; human; fig 4
| Chow H, Dong B, Duron S, Campbell D, Ong C, Hoeflich K, et al. Group I Paks as therapeutic targets in NF2-deficient meningioma. Oncotarget. 2015;6:1981-94 pubmed |
- western blot; mouse; fig 5
| Zhang W, Hou J, Wang X, Jiang R, Yin Y, Ji J, et al. PTPRO-mediated autophagy prevents hepatosteatosis and tumorigenesis. Oncotarget. 2015;6:9420-33 pubmed |
- western blot; human; fig s4
| Kocher B, White L, Piwnica-Worms D. DAPK3 suppresses acini morphogenesis and is required for mouse development. Mol Cancer Res. 2015;13:358-67 pubmed publisher |
- western blot; human; 1:1000; fig 1b
| Yu J, Ramasamy T, Murphy N, Holt M, Czapiewski R, Wei S, et al. PI3K/mTORC2 regulates TGF-β/Activin signalling by modulating Smad2/3 activity via linker phosphorylation. Nat Commun. 2015;6:7212 pubmed publisher |
- western blot; human; fig 6
| Albers P, Bojsen-Møller K, Dirksen C, Serup A, Kristensen D, Frystyk J, et al. Enhanced insulin signaling in human skeletal muscle and adipose tissue following gastric bypass surgery. Am J Physiol Regul Integr Comp Physiol. 2015;309:R510-24 pubmed publisher |
- western blot; human; fig 5
| Meyer K, Albaugh B, Schoenike B, Roopra A. Type 1 Insulin-Like Growth Factor Receptor/Insulin Receptor Substrate 1 Signaling Confers Pathogenic Activity on Breast Tumor Cells Lacking REST. Mol Cell Biol. 2015;35:2991-3004 pubmed publisher |
- flow cytometry; human; 1:250
| Hellesøy M, Lorens J. Cellular context-mediated Akt dynamics regulates MAP kinase signaling thresholds during angiogenesis. Mol Biol Cell. 2015;26:2698-711 pubmed publisher |
- western blot; human; fig 1
| Newsom S, Everett A, Park S, Van Pelt D, Hinko A, Horowitz J. Lipid mixtures containing a very high proportion of saturated fatty acids only modestly impair insulin signaling in cultured muscle cells. PLoS ONE. 2015;10:e0120871 pubmed publisher |
- western blot; pig; 1:1000
| Columbus D, Steinhoff-Wagner J, Suryawan A, Nguyen H, Hernandez-Garcia A, Fiorotto M, et al. Impact of prolonged leucine supplementation on protein synthesis and lean growth in neonatal pigs. Am J Physiol Endocrinol Metab. 2015;309:E601-10 pubmed publisher |
- western blot; mouse; fig 5
| Giampietro C, Disanza A, Bravi L, Barrios-Rodiles M, Corada M, Frittoli E, et al. The actin-binding protein EPS8 binds VE-cadherin and modulates YAP localization and signaling. J Cell Biol. 2015;211:1177-92 pubmed publisher |
- western blot; mouse; fig 5
| Pulinilkunnil T, Kienesberger P, Nagendran J, Sharma N, Young M, Dyck J. Cardiac-specific adipose triglyceride lipase overexpression protects from cardiac steatosis and dilated cardiomyopathy following diet-induced obesity. Int J Obes (Lond). 2014;38:205-15 pubmed publisher |
| Ito Y, Hart J, Ueno L, Vogt P. Oncogenic activity of the regulatory subunit p85β of phosphatidylinositol 3-kinase (PI3K). Proc Natl Acad Sci U S A. 2014;111:16826-9 pubmed publisher |
| Glorieux C, Auquier J, Dejeans N, Sid B, Demoulin J, Bertrand L, et al. Catalase expression in MCF-7 breast cancer cells is mainly controlled by PI3K/Akt/mTor signaling pathway. Biochem Pharmacol. 2014;89:217-23 pubmed publisher |
- western blot; mouse; 1:1000
| Yu M, Trobridge P, Wang Y, Kanngurn S, Morris S, Knoblaugh S, et al. Inactivation of TGF-β signaling and loss of PTEN cooperate to induce colon cancer in vivo. Oncogene. 2014;33:1538-47 pubmed publisher |
- western blot; human; 1:1000; fig 3
| Lebron M, Brennan L, Damoci C, Prewett M, O'Mahony M, Duignan I, et al. A human monoclonal antibody targeting the stem cell factor receptor (c-Kit) blocks tumor cell signaling and inhibits tumor growth. Cancer Biol Ther. 2014;15:1208-18 pubmed publisher |
- western blot; mouse; fig 3
| Tan S, Shui G, Zhou J, Shi Y, Huang J, Xia D, et al. Critical role of SCD1 in autophagy regulation via lipogenesis and lipid rafts-coupled AKT-FOXO1 signaling pathway. Autophagy. 2014;10:226-42 pubmed publisher |
- western blot; mouse; fig 6
| Tang S, Chen T, Yu Z, Zhu X, Yang M, Xie B, et al. RasGRP3 limits Toll-like receptor-triggered inflammatory response in macrophages by activating Rap1 small GTPase. Nat Commun. 2014;5:4657 pubmed publisher |
- western blot; human; fig 5
| Mashukova A, Kozhekbaeva Z, Forteza R, Dulam V, Figueroa Y, Warren R, et al. The BAG-1 isoform BAG-1M regulates keratin-associated Hsp70 chaperoning of aPKC in intestinal cells during activation of inflammatory signaling. J Cell Sci. 2014;127:3568-77 pubmed publisher |
- western blot; mouse; 1:1000; fig 3, 5
| Wadosky K, Rodriguez J, Hite R, Min J, Walton B, Willis M. Muscle RING finger-1 attenuates IGF-I-dependent cardiomyocyte hypertrophy by inhibiting JNK signaling. Am J Physiol Endocrinol Metab. 2014;306:E723-39 pubmed publisher |
| Wu T, Ye Y, Min S, Zhu J, Khobahy E, Zhou J, et al. Prevention of murine lupus nephritis by targeting multiple signaling axes and oxidative stress using a synthetic triterpenoid. Arthritis Rheumatol. 2014;66:3129-39 pubmed publisher |
- flow cytometry; human; 1:50
| Hellesøy M, Blois A, Tiron C, Mannelqvist M, Akslen L, Lorens J. Akt1 activity regulates vessel maturation in a tissue engineering model of angiogenesis. Tissue Eng Part A. 2014;20:2590-603 pubmed publisher |
| Sharma N, Sequea D, Castorena C, Arias E, Qi N, Cartee G. Heterogeneous effects of calorie restriction on in vivo glucose uptake and insulin signaling of individual rat skeletal muscles. PLoS ONE. 2014;8:e65118 pubmed publisher |
- western blot; human; fig 1
| Gasser J, Inuzuka H, Lau A, Wei W, Beroukhim R, Toker A. SGK3 mediates INPP4B-dependent PI3K signaling in breast cancer. Mol Cell. 2014;56:595-607 pubmed publisher |
- western blot; human; 1:1,000
| Gu L, Talati P, Vogiatzi P, Romero-Weaver A, Abdulghani J, Liao Z, et al. Pharmacologic suppression of JAK1/2 by JAK1/2 inhibitor AZD1480 potently inhibits IL-6-induced experimental prostate cancer metastases formation. Mol Cancer Ther. 2014;13:1246-58 pubmed publisher |
| El-Ami T, Moll L, Carvalhal Marques F, Volovik Y, Reuveni H, Cohen E. A novel inhibitor of the insulin/IGF signaling pathway protects from age-onset, neurodegeneration-linked proteotoxicity. Aging Cell. 2014;13:165-74 pubmed publisher |
| Valente A, Irimpen A, Siebenlist U, Chandrasekar B. OxLDL induces endothelial dysfunction and death via TRAF3IP2: inhibition by HDL3 and AMPK activators. Free Radic Biol Med. 2014;70:117-28 pubmed publisher |
- western blot; human; 1:2000
| Xiang M, Birkbak N, Vafaizadeh V, Walker S, Yeh J, Liu S, et al. STAT3 induction of miR-146b forms a feedback loop to inhibit the NF-κB to IL-6 signaling axis and STAT3-driven cancer phenotypes. Sci Signal. 2014;7:ra11 pubmed publisher |
- immunohistochemistry - paraffin section; human; 1:800
- western blot; human
| Kucherlapati M, Esfahani S, Habibollahi P, Wang J, Still E, Bronson R, et al. Genotype directed therapy in murine mismatch repair deficient tumors. PLoS ONE. 2013;8:e68817 pubmed publisher |
| Sáinz-Jaspeado M, Huertas-Martinez J, Lagares-Tena L, Martín Liberal J, Mateo-Lozano S, de Alava E, et al. EphA2-induced angiogenesis in ewing sarcoma cells works through bFGF production and is dependent on caveolin-1. PLoS ONE. 2013;8:e71449 pubmed publisher |
- western blot; mouse; 1:500
| Udagawa T, Farny N, Jakovcevski M, Kaphzan H, Alarcon J, Anilkumar S, et al. Genetic and acute CPEB1 depletion ameliorate fragile X pathophysiology. Nat Med. 2013;19:1473-7 pubmed publisher |
| Landry W, Woolley J, Cotter T. Imatinib and Nilotinib inhibit Bcr-Abl-induced ROS through targeted degradation of the NADPH oxidase subunit p22phox. Leuk Res. 2013;37:183-9 pubmed publisher |
| Paugh B, Zhu X, Qu C, Endersby R, Diaz A, Zhang J, et al. Novel oncogenic PDGFRA mutations in pediatric high-grade gliomas. Cancer Res. 2013;73:6219-29 pubmed publisher |
- western blot; rat; 1:1000
| Hou Z, Nie C, Si Z, Ma Y. Deferoxamine enhances neovascularization and accelerates wound healing in diabetic rats via the accumulation of hypoxia-inducible factor-1α. Diabetes Res Clin Pract. 2013;101:62-71 pubmed publisher |
| Neukamm S, Ott J, Dammeier S, Lehmann R, Haring H, Schleicher E, et al. Phosphorylation of serine 1137/1138 of mouse insulin receptor substrate (IRS) 2 regulates cAMP-dependent binding to 14-3-3 proteins and IRS2 protein degradation. J Biol Chem. 2013;288:16403-15 pubmed publisher |
| Sharma N, Arias E, Sequea D, Cartee G. Preventing the calorie restriction-induced increase in insulin-stimulated Akt2 phosphorylation eliminates calorie restriction's effect on glucose uptake in skeletal muscle. Biochim Biophys Acta. 2012;1822:1735-40 pubmed publisher |
| Schweitzer G, Arias E, Cartee G. Sustained postexercise increases in AS160 Thr642 and Ser588 phosphorylation in skeletal muscle without sustained increases in kinase phosphorylation. J Appl Physiol (1985). 2012;113:1852-61 pubmed publisher |
| O'Brien T, Gorentla B, Xie D, Srivatsan S, McLeod I, He Y, et al. Regulation of T-cell survival and mitochondrial homeostasis by TSC1. Eur J Immunol. 2011;41:3361-70 pubmed publisher |
| Ong Q, Guo S, Duan L, Zhang K, Collier E, Cui B. The Timing of Raf/ERK and AKT Activation in Protecting PC12 Cells against Oxidative Stress. PLoS ONE. 2016;11:e0153487 pubmed publisher |
| Kim R, Cui Y, Yoo K, Kim I, Lee M, Choi Y, et al. Radiation promotes malignant phenotypes through SRC in breast cancer cells. Cancer Sci. 2015;106:78-85 pubmed publisher |
| Matsuda-Lennikov M, Suizu F, Hirata N, Hashimoto M, Kimura K, Nagamine T, et al. Lysosomal interaction of Akt with Phafin2: a critical step in the induction of autophagy. PLoS ONE. 2014;9:e79795 pubmed publisher |
| Chen C, Chen K, Yang T, Liu H, Hsu S. Gallic Acid Induces a Reactive Oxygen Species-Provoked c-Jun NH2-Terminal Kinase-Dependent Apoptosis in Lung Fibroblasts. Evid Based Complement Alternat Med. 2013;2013:613950 pubmed publisher |
| Ponnurangam S, Standing D, Rangarajan P, Subramaniam D. Tandutinib inhibits the Akt/mTOR signaling pathway to inhibit colon cancer growth. Mol Cancer Ther. 2013;12:598-609 pubmed publisher |
| Zhang W, Wang Q, Song P, Zou M. Liver kinase b1 is required for white adipose tissue growth and differentiation. Diabetes. 2013;62:2347-58 pubmed publisher |
| Rachakonda G, Sekhar K, Jowhar D, Samson P, Wikswo J, Beauchamp R, et al. Increased cell migration and plasticity in Nrf2-deficient cancer cell lines. Oncogene. 2010;29:3703-14 pubmed publisher |
| Kawase T, Ohki R, Shibata T, Tsutsumi S, Kamimura N, Inazawa J, et al. PH domain-only protein PHLDA3 is a p53-regulated repressor of Akt. Cell. 2009;136:535-50 pubmed publisher |
| Fan Y, Sun C, Gao X, Wang F, Li X, Kassim R, et al. Neuroprotective effects of ginseng pectin through the activation of ERK/MAPK and Akt survival signaling pathways. Mol Med Rep. 2012;5:1185-90 pubmed publisher |
| Ben-Ami I, Yao Z, Naor Z, Seger R. Gq protein-induced apoptosis is mediated by AKT kinase inhibition that leads to protein kinase C-induced c-Jun N-terminal kinase activation. J Biol Chem. 2011;286:31022-31 pubmed publisher |
| Kim T, Franco H, Jung S, Qin J, Broaddus R, Lydon J, et al. The synergistic effect of Mig-6 and Pten ablation on endometrial cancer development and progression. Oncogene. 2010;29:3770-80 pubmed publisher |
