Akt Antibody | Cell Signaling Technology 9272S product information

product summary

company name :

Cell Signaling Technology

product type :

antibody

product name :

Akt Antibody

catalog :

9272S

quantity :

100 ul

price :

235 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat, dog, pig, fruit fly

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - free floating section

more info or order :

Cell Signaling Technology product webpage

citations: 154

Published Application/Species/Dilution	Reference
western blot; human; 1:1000; fig 3	Bao H, Liu P, Jiang K, Zhang X, Xie L, Wang Z, et al. Huaier polysaccharide induces apoptosis in hepatocellular carcinoma cells through p38 MAPK. Oncol Lett. 2016;12:1058-1066 pubmed
western blot; human; fig 2	Fearnley G, Smith G, Abdul-Zani I, Yuldasheva N, Mughal N, Homer-Vanniasinkam S, et al. VEGF-A isoforms program differential VEGFR2 signal transduction, trafficking and proteolysis. Biol Open. 2016;5:571-83 pubmed publisher
western blot; rat	Ronchi G, Haastert-Talini K, Fornasari B, Perroteau I, Geuna S, Gambarotta G. The Neuregulin1/ErbB system is selectively regulated during peripheral nerve degeneration and regeneration. Eur J Neurosci. 2016;43:351-64 pubmed publisher
western blot; mouse; fig 7	Park J, Kotani T, Konno T, Setiawan J, Kitamura Y, Imada S, et al. Promotion of Intestinal Epithelial Cell Turnover by Commensal Bacteria: Role of Short-Chain Fatty Acids. PLoS ONE. 2016;11:e0156334 pubmed publisher
western blot; human; 1:1000; fig 3 western blot; mouse; 1:1000; fig 1	Takabatake Y, Oxvig C, Nagi C, Adelson K, Jaffer S, Schmidt H, et al. Lactation opposes pappalysin-1-driven pregnancy-associated breast cancer. EMBO Mol Med. 2016;8:388-406 pubmed publisher
western blot; rat; 1:500; fig 5	Xu Y, Liu J, He M, Liu R, Belegu V, Dai P, et al. Mechanisms of PDGF siRNA-mediated inhibition of bone cancer pain in the spinal cord. Sci Rep. 2016;6:27512 pubmed publisher
western blot; mouse; 1:200	Zarpelon A, Rodrigues F, Lopes A, Souza G, Carvalho T, Pinto L, et al. Spinal cord oligodendrocyte-derived alarmin IL-33 mediates neuropathic pain. FASEB J. 2016;30:54-65 pubmed publisher
western blot; mouse; fig 2	Hinds T, Burns K, Hosick P, McBeth L, Nestor-Kalinoski A, Drummond H, et al. Biliverdin Reductase A Attenuates Hepatic Steatosis by Inhibition of Glycogen Synthase Kinase (GSK) 3β Phosphorylation of Serine 73 of Peroxisome Proliferator-activated Receptor (PPAR) α. J Biol Chem. 2016;291:25179-25191 pubmed
western blot; rat; 1:1000; fig 1	Chunchai T, Samniang B, Sripetchwandee J, Pintana H, Pongkan W, Kumfu S, et al. Vagus Nerve Stimulation Exerts the Neuroprotective Effects in Obese-Insulin Resistant Rats, Leading to the Improvement of Cognitive Function. Sci Rep. 2016;6:26866 pubmed publisher
western blot; human; fig 4	Lyabin D, Ovchinnikov L. Selective regulation of YB-1 mRNA translation by the mTOR signaling pathway is not mediated by 4E-binding protein. Sci Rep. 2016;6:22502 pubmed publisher
western blot; mouse; fig 4	Li M, Lu G, Hu J, Shen X, Ju J, Gao Y, et al. EVA1A/TMEM166 Regulates Embryonic Neurogenesis by Autophagy. Stem Cell Reports. 2016;6:396-410 pubmed publisher
western blot; mouse; 1:1000; fig 8	Gurnik S, Devraj K, Macas J, Yamaji M, Starke J, Scholz A, et al. Angiopoietin-2-induced blood-brain barrier compromise and increased stroke size are rescued by VE-PTP-dependent restoration of Tie2 signaling. Acta Neuropathol. 2016;131:753-73 pubmed publisher
western blot; human; fig 4	Kabe Y, Nakane T, Koike I, Yamamoto T, Sugiura Y, Harada E, et al. Haem-dependent dimerization of PGRMC1/Sigma-2 receptor facilitates cancer proliferation and chemoresistance. Nat Commun. 2016;7:11030 pubmed publisher
western blot; human	Wang Y, Yang R, Gu J, Yin X, Jin N, Xie S, et al. Cross talk between PI3K-AKT-GSK-3β and PP2A pathways determines tau hyperphosphorylation. Neurobiol Aging. 2015;36:188-200 pubmed publisher
western blot; human; fig 2	Wu B, Wang Y, Yang X, Xu B, Feng F, Wang B, et al. Basigin-mediated redistribution of CD98 promotes cell spreading and tumorigenicity in hepatocellular carcinoma. J Exp Clin Cancer Res. 2015;34:110 pubmed publisher
western blot; mouse; fig 3	Yamakawa H, Muraoka N, Miyamoto K, Sadahiro T, Isomi M, Haginiwa S, et al. Fibroblast Growth Factors and Vascular Endothelial Growth Factor Promote Cardiac Reprogramming under Defined Conditions. Stem Cell Reports. 2015;5:1128-42 pubmed publisher
western blot; human; 1:1000; fig 1e	Ding B, Gomi K, Rafii S, Crystal R, Walters M. Endothelial MMP14 is required for endothelial-dependent growth support of human airway basal cells. J Cell Sci. 2015;128:2983-8 pubmed publisher
western blot; human	Stohr R, Mavilio M, Marino A, Casagrande V, Kappel B, Möllmann J, et al. ITCH modulates SIRT6 and SREBP2 to influence lipid metabolism and atherosclerosis in ApoE null mice. Sci Rep. 2015;5:9023 pubmed publisher
immunocytochemistry; human western blot; rat	Zhang L, Wang H, Ding K, Xu J. FTY720 induces autophagy-related apoptosis and necroptosis in human glioblastoma cells. Toxicol Lett. 2015;236:43-59 pubmed publisher
western blot; human; 1:500	Barrichon M, Hadi T, Wendremaire M, Ptasinski C, Seigneuric R, Marcion G, et al. Dose-dependent biphasic leptin-induced proliferation is caused by non-specific IL-6/NF-κB pathway activation in human myometrial cells. Br J Pharmacol. 2015;172:2974-90 pubmed publisher
western blot; fruit fly; 1:1000	Cuesto G, Jordán-Álvarez S, Enriquez-Barreto L, Ferrús A, Morales M, Acebes A. GSK3β inhibition promotes synaptogenesis in Drosophila and mammalian neurons. PLoS ONE. 2015;10:e0118475 pubmed publisher
western blot; mouse	Azimzadeh O, Sievert W, Sarioglu H, Merl-Pham J, Yentrapalli R, Bakshi M, et al. Integrative proteomics and targeted transcriptomics analyses in cardiac endothelial cells unravel mechanisms of long-term radiation-induced vascular dysfunction. J Proteome Res. 2015;14:1203-19 pubmed publisher
western blot; pig; 1:1000	Columbus D, Steinhoff-Wagner J, Suryawan A, Nguyen H, Hernandez-Garcia A, Fiorotto M, et al. Impact of prolonged leucine supplementation on protein synthesis and lean growth in neonatal pigs. Am J Physiol Endocrinol Metab. 2015;309:E601-10 pubmed publisher
western blot; mouse; 1:2000	Dahlhoff M, Schafer M, Muzumdar S, Rose C, Schneider M. ERBB3 is required for tumor promotion in a mouse model of skin carcinogenesis. Mol Oncol. 2015;9:1825-33 pubmed publisher
western blot; human; 1:1000	Zhang X, Cheng S, Bian K, Wang L, Zhang X, Yan B, et al. MicroRNA-26a promotes anoikis in human hepatocellular carcinoma cells by targeting alpha5 integrin. Oncotarget. 2015;6:2277-89 pubmed
immunohistochemistry - free floating section; rat	Gorojod R, Alaimo A, Porte Alcon S, Pomilio C, Saravia F, Kotler M. The autophagic- lysosomal pathway determines the fate of glial cells under manganese- induced oxidative stress conditions. Free Radic Biol Med. 2015;87:237-51 pubmed publisher
western blot; human; 1:1000	Qu D, Chen Y, Xu X, Zhang M, Zhang Y, Li S. Cbl-b-regulated extracellular signal-regulated kinase signaling is involved in the shikonin-induced apoptosis of lung cancer cells in vitro. Exp Ther Med. 2015;9:1265-1270 pubmed
western blot; human; 1:1000	Zhang W, Cai J, Chen S, Zheng X, Hu S, Dong W, et al. Paclitaxel resistance in MCF-7/PTX cells is reversed by paeonol through suppression of the SET/phosphatidylinositol 3-kinase/Akt pathway. Mol Med Rep. 2015;12:1506-14 pubmed publisher
western blot; mouse; 1:1000	Crane J, Palanivel R, Mottillo E, Bujak A, Wang H, Ford R, et al. Inhibiting peripheral serotonin synthesis reduces obesity and metabolic dysfunction by promoting brown adipose tissue thermogenesis. Nat Med. 2015;21:166-72 pubmed publisher
western blot; mouse	McGowan S, McCoy D. Fibroblast growth factor signaling in myofibroblasts differs from lipofibroblasts during alveolar septation in mice. Am J Physiol Lung Cell Mol Physiol. 2015;309:L463-74 pubmed publisher
western blot; mouse	Andersson L, Scharin Tang M, Lundqvist A, Lindbom M, Mardani I, Fogelstrand P, et al. Rip2 modifies VEGF-induced signalling and vascular permeability in myocardial ischaemia. Cardiovasc Res. 2015;107:478-86 pubmed publisher
western blot; human; fig 4	Chow H, Dong B, Duron S, Campbell D, Ong C, Hoeflich K, et al. Group I Paks as therapeutic targets in NF2-deficient meningioma. Oncotarget. 2015;6:1981-94 pubmed
western blot; rat; 1:1000	Pérez-Alvarez M, Mateos L, Alonso A, Wandosell F. Estradiol and Progesterone Administration After pMCAO Stimulates the Neurological Recovery and Reduces the Detrimental Effect of Ischemia Mainly in Hippocampus. Mol Neurobiol. 2015;52:1690-703 pubmed publisher
western blot; human	Roffe M, Lupinacci F, Soares L, Hajj G, Martins V. Two widely used RSK inhibitors, BI-D1870 and SL0101, alter mTORC1 signaling in a RSK-independent manner. Cell Signal. 2015;27:1630-42 pubmed publisher
western blot; mouse; fig 3 immunoprecipitation; mouse; fig 4	da Rocha A, Pereira B, Pauli J, Cintra D, De Souza C, Ropelle E, et al. Downhill Running-Based Overtraining Protocol Improves Hepatic Insulin Signaling Pathway without Concomitant Decrease of Inflammatory Proteins. PLoS ONE. 2015;10:e0140020 pubmed publisher
western blot; human; fig 1	Hausmann S, Brandt E, Köchel C, Einsele H, Bargou R, Seggewiss-Bernhardt R, et al. Loss of serum and glucocorticoid-regulated kinase 3 (SGK3) does not affect proliferation and survival of multiple myeloma cell lines. PLoS ONE. 2015;10:e0122689 pubmed publisher
western blot; mouse immunohistochemistry; mouse	Tan X, Xue Y, Ma T, Wang X, Li J, Lan L, et al. Partial eNOS deficiency causes spontaneous thrombotic cerebral infarction, amyloid angiopathy and cognitive impairment. Mol Neurodegener. 2015;10:24 pubmed publisher
western blot; mouse; 0.025 ug/ml; fig 4	Cookman C, Belcher S. Estrogen Receptor-β Up-Regulates IGF1R Expression and Activity to Inhibit Apoptosis and Increase Growth of Medulloblastoma. Endocrinology. 2015;156:2395-408 pubmed publisher
western blot; mouse; 1:1000; fig 4	Zhou L, Park S, Xu L, Xia X, Ye J, Su L, et al. Insulin resistance and white adipose tissue inflammation are uncoupled in energetically challenged Fsp27-deficient mice. Nat Commun. 2015;6:5949 pubmed publisher
western blot; mouse	Lin T, Shih Y, Chen S, Lien C, Chang C, Huang T, et al. Running exercise delays neurodegeneration in amygdala and hippocampus of Alzheimer's disease (APP/PS1) transgenic mice. Neurobiol Learn Mem. 2015;118:189-97 pubmed publisher
western blot; mouse; fig 7	Bettaieb A, Jiang J, Sasaki Y, Chao T, Kiss Z, Chen X, et al. Hepatocyte Nicotinamide Adenine Dinucleotide Phosphate Reduced Oxidase 4 Regulates Stress Signaling, Fibrosis, and Insulin Sensitivity During Development of Steatohepatitis in Mice. Gastroenterology. 2015;149:468-80.e10 pubmed publisher
western blot; human	Liu Z, Jiang C, Zhang J, Liu B, Du Q. Resveratrol inhibits inflammation and ameliorates insulin resistant endothelial dysfunction via regulation of AMP-activated protein kinase and sirtuin 1 activities. J Diabetes. 2015;: pubmed publisher
western blot; human; fig s4	Diesenberg K, Beerbaum M, Fink U, Schmieder P, Krauss M. SEPT9 negatively regulates ubiquitin-dependent downregulation of EGFR. J Cell Sci. 2015;128:397-407 pubmed publisher
western blot; human	Gorantla S, Zirlik K, Reiter A, Yu C, Illert A, von Bubnoff N, et al. F604S exchange in FIP1L1-PDGFRA enhances FIP1L1-PDGFRA protein stability via SHP-2 and SRC: a novel mode of kinase inhibitor resistance. Leukemia. 2015;29:1763-70 pubmed publisher
western blot; rat	Park B, Cha S, Han B, Kim S. Angiotensin IV stimulates high atrial stretch-induced ANP secretion via insulin regulated aminopeptidase. Peptides. 2015;63:30-7 pubmed publisher
western blot; human	Picot N, Guerrette R, Beauregard A, Jean S, Michaud P, Harquail J, et al. Mammaglobin 1 promotes breast cancer malignancy and confers sensitivity to anticancer drugs. Mol Carcinog. 2015;: pubmed publisher
western blot; mouse	Seo K, Lee S, Ye B, Kim Y, Bae S, Kim C. Mechanical stretch enhances the expression and activity of osteopontin and MMP-2 via the Akt1/AP-1 pathways in VSMC. J Mol Cell Cardiol. 2015;85:13-24 pubmed publisher
western blot; human; 1:1000	Li C, Siragy H. (Pro)renin receptor regulates autophagy and apoptosis in podocytes exposed to high glucose. Am J Physiol Endocrinol Metab. 2015;309:E302-10 pubmed publisher
western blot; human; 1:1000	Kim E, Kim S, Jin X, Ham S, Kim J, Park J, et al. Epidermal growth factor receptor variant III renders glioma cancer cells less differentiated by JAGGED1. Tumour Biol. 2015;36:2921-8 pubmed publisher
western blot; human	Fiorini C, Cordani M, Gotte G, Picone D, Donadelli M. Onconase induces autophagy sensitizing pancreatic cancer cells to gemcitabine and activates Akt/mTOR pathway in a ROS-dependent manner. Biochim Biophys Acta. 2015;1853:549-60 pubmed publisher
western blot; mouse; fig 4	Petrov D, Pedrós I, Artiach G, Sureda F, Barroso E, Pallas M, et al. High-fat diet-induced deregulation of hippocampal insulin signaling and mitochondrial homeostasis deficiences contribute to Alzheimer disease pathology in rodents. Biochim Biophys Acta. 2015;1852:1687-99 pubmed publisher
western blot; rat; fig 4	Zhang Z, Zhang T, Zhou Y, Wei X, Zhu J, Zhang J, et al. Activated phosphatidylinositol 3-kinase/Akt inhibits the transition of endothelial progenitor cells to mesenchymal cells by regulating the forkhead box subgroup O-3a signaling. Cell Physiol Biochem. 2015;35:1643-53 pubmed publisher
western blot; human; fig 3, 4	Puig M, Lugo R, Gabasa M, Giménez A, Velasquez A, Galgoczy R, et al. Matrix stiffening and β1 integrin drive subtype-specific fibroblast accumulation in lung cancer. Mol Cancer Res. 2015;13:161-73 pubmed publisher
western blot; human	Morancho B, Martinez-Barriocanal A, Villanueva J, Arribas J. Role of ADAM17 in the non-cell autonomous effects of oncogene-induced senescence. Breast Cancer Res. 2015;17:106 pubmed publisher
western blot; human	Prickaerts P, Niessen H, Dahlmans V, Spaapen F, Salvaing J, Vanhove J, et al. MK3 modulation affects BMI1-dependent and independent cell cycle check-points. PLoS ONE. 2015;10:e0118840 pubmed publisher
western blot; human; fig 3	Zhou Q, Derti A, Ruddy D, Rakiec D, Kao I, Lira M, et al. A chemical genetics approach for the functional assessment of novel cancer genes. Cancer Res. 2015;75:1949-58 pubmed publisher
western blot; mouse; 1:1000; fig 5b	Murrow L, Malhotra R, Debnath J. ATG12-ATG3 interacts with Alix to promote basal autophagic flux and late endosome function. Nat Cell Biol. 2015;17:300-10 pubmed publisher
western blot; human; fig 5	Meyer K, Albaugh B, Schoenike B, Roopra A. Type 1 Insulin-Like Growth Factor Receptor/Insulin Receptor Substrate 1 Signaling Confers Pathogenic Activity on Breast Tumor Cells Lacking REST. Mol Cell Biol. 2015;35:2991-3004 pubmed publisher
western blot; rat; 1:1000	García-Pérez D, Laorden M, Milanés M. Regulation of Pleiotrophin, Midkine, Receptor Protein Tyrosine Phosphatase β/ζ, and Their Intracellular Signaling Cascades in the Nucleus Accumbens During Opiate Administration. Int J Neuropsychopharmacol. 2015;: pubmed publisher
western blot; human	Wang J, Mikse O, Liao R, Li Y, Tan L, Jänne P, et al. Ligand-associated ERBB2/3 activation confers acquired resistance to FGFR inhibition in FGFR3-dependent cancer cells. Oncogene. 2015;34:2167-77 pubmed publisher
western blot; human; fig 6f	Wu H, Hwang-Verslues W, Lee W, Huang C, Wei P, Chen C, et al. Targeting IL-17B-IL-17RB signaling with an anti-IL-17RB antibody blocks pancreatic cancer metastasis by silencing multiple chemokines. J Exp Med. 2015;212:333-49 pubmed publisher
western blot; human; 1:1000	WILLIAMS K, KILLEBREW D, Clary G, Seawell J, Meeker R. Differential regulation of macrophage phenotype by mature and pro-nerve growth factor. J Neuroimmunol. 2015;285:76-93 pubmed publisher
western blot; rat	Souza R, Piedade W, Soares L, Souza P, Aguiar A, Vechetti-Júnior I, et al. Aerobic exercise training prevents heart failure-induced skeletal muscle atrophy by anti-catabolic, but not anabolic actions. PLoS ONE. 2014;9:e110020 pubmed publisher
western blot; mouse	Marquez E, Riera M, Pascual J, Soler M. Albumin inhibits the insulin-mediated ACE2 increase in cultured podocytes. Am J Physiol Renal Physiol. 2014;306:F1327-34 pubmed publisher
western blot; human; 1:1000	Haas M, Onstead-Haas L, Naem E, Arnold A, Rohrbaugh N, Flowers M, et al. The effect of black seed (Nigella sativa) extract on FOXO3 expression in HepG2 cells. Phytother Res. 2014;28:873-9 pubmed publisher
western blot; human	Ozek C, Kanoski S, Zhang Z, Grill H, Bence K. Protein-tyrosine phosphatase 1B (PTP1B) is a novel regulator of central brain-derived neurotrophic factor and tropomyosin receptor kinase B (TrkB) signaling. J Biol Chem. 2014;289:31682-92 pubmed publisher
western blot; human; 1:1000; fig 7	Naudin C, Sirvent A, Leroy C, Larive R, Simon V, Pannequin J, et al. SLAP displays tumour suppressor functions in colorectal cancer via destabilization of the SRC substrate EPHA2. Nat Commun. 2014;5:3159 pubmed publisher
western blot; mouse; fig 3a	Zheng X, Zhai B, Koivunen P, Shin S, Lu G, Liu J, et al. Prolyl hydroxylation by EglN2 destabilizes FOXO3a by blocking its interaction with the USP9x deubiquitinase. Genes Dev. 2014;28:1429-44 pubmed publisher
western blot; rat; 1:500; fig 5	Scotti L, Abramovich D, Pascuali N, Irusta G, Meresman G, Tesone M, et al. Local VEGF inhibition prevents ovarian alterations associated with ovarian hyperstimulation syndrome. J Steroid Biochem Mol Biol. 2014;144:392-401 pubmed publisher
western blot; human; 1:2000	Xiang M, Birkbak N, Vafaizadeh V, Walker S, Yeh J, Liu S, et al. STAT3 induction of miR-146b forms a feedback loop to inhibit the NF-κB to IL-6 signaling axis and STAT3-driven cancer phenotypes. Sci Signal. 2014;7:ra11 pubmed publisher
western blot; dog	Chen S, Otero Y, Mulligan K, Lundblad T, Williams P, McGuinness O. Liver, but not muscle, has an entrainable metabolic memory. PLoS ONE. 2014;9:e86164 pubmed publisher
western blot; human; 1:1000	Bouchekioua-Bouzaghou K, Poulard C, Rambaud J, Lavergne E, Hussein N, Billaud M, et al. LKB1 when associated with methylatedERα is a marker of bad prognosis in breast cancer. Int J Cancer. 2014;135:1307-18 pubmed publisher
western blot; human	Wan Y, Yang Y, Leng Q, Lan B, Jia H, Liu Y, et al. Vav1 increases Bcl-2 expression by selective activation of Rac2-Akt in leukemia T cells. Cell Signal. 2014;26:2202-9 pubmed publisher
western blot; rat; 1:1000	Padiya R, Chowdhury D, Borkar R, Srinivas R, Pal Bhadra M, Banerjee S. Garlic attenuates cardiac oxidative stress via activation of PI3K/AKT/Nrf2-Keap1 pathway in fructose-fed diabetic rat. PLoS ONE. 2014;9:e94228 pubmed publisher
western blot; human	Xu C, Chen H, Wang X, Gao J, Che Y, Li Y, et al. S100A14, a member of the EF-hand calcium-binding proteins, is overexpressed in breast cancer and acts as a modulator of HER2 signaling. J Biol Chem. 2014;289:827-37 pubmed publisher
western blot; human	Son J, Jeong H, Kim H, Kim Y, Lee E, Lee H, et al. Pelargonidin attenuates PDGF-BB-induced aortic smooth muscle cell proliferation and migration by direct inhibition of focal adhesion kinase. Biochem Pharmacol. 2014;89:236-45 pubmed publisher
western blot; human	Watanabe T, Nakamura S, Ono T, Ui S, Yagi S, Kagawa H, et al. Pyrrolidinium fullerene induces apoptosis by activation of procaspase-9 via suppression of Akt in primary effusion lymphoma. Biochem Biophys Res Commun. 2014;451:93-100 pubmed publisher
western blot; human	Beltran P, Calzone F, Mitchell P, Chung Y, Cajulis E, Moody G, et al. Ganitumab (AMG 479) inhibits IGF-II-dependent ovarian cancer growth and potentiates platinum-based chemotherapy. Clin Cancer Res. 2014;20:2947-58 pubmed publisher
western blot; mouse; 1:1000	Ribeiro M, Rosenstock T, Oliveira A, Oliveira C, Rego A. Insulin and IGF-1 improve mitochondrial function in a PI-3K/Akt-dependent manner and reduce mitochondrial generation of reactive oxygen species in Huntington's disease knock-in striatal cells. Free Radic Biol Med. 2014;74:129-44 pubmed publisher
western blot; human; fig 4	Tan L, Wang J, Tanizaki J, Huang Z, Aref A, Rusan M, et al. Development of covalent inhibitors that can overcome resistance to first-generation FGFR kinase inhibitors. Proc Natl Acad Sci U S A. 2014;111:E4869-77 pubmed publisher
western blot; human	Czaplinska D, Turczyk L, Grudowska A, Mieszkowska M, Lipinska A, Skladanowski A, et al. Phosphorylation of RSK2 at Tyr529 by FGFR2-p38 enhances human mammary epithelial cells migration. Biochim Biophys Acta. 2014;1843:2461-70 pubmed publisher
western blot; mouse; 1:1000	Barns M, Gondro C, Tellam R, Radley-Crabb H, Grounds M, Shavlakadze T. Molecular analyses provide insight into mechanisms underlying sarcopenia and myofibre denervation in old skeletal muscles of mice. Int J Biochem Cell Biol. 2014;53:174-85 pubmed publisher
western blot; rat	Yi X, Li X, Zhou Y, Ren S, Wan W, Feng G, et al. Hepatocyte growth factor regulates the TGF-β1-induced proliferation, differentiation and secretory function of cardiac fibroblasts. Int J Mol Med. 2014;34:381-90 pubmed publisher
western blot; human	Kugel C, Hartsough E, Davies M, Setiady Y, Aplin A. Function-blocking ERBB3 antibody inhibits the adaptive response to RAF inhibitor. Cancer Res. 2014;74:4122-32 pubmed publisher
western blot; mouse; 1:1000	Majumder M, Xin X, Liu L, Girish G, Lala P. Prostaglandin E2 receptor EP4 as the common target on cancer cells and macrophages to abolish angiogenesis, lymphangiogenesis, metastasis, and stem-like cell functions. Cancer Sci. 2014;105:1142-51 pubmed publisher
western blot; rat; 1:1000	Bhattachariya A, Turczynska K, Grossi M, Nordström I, Buckbinder L, Albinsson S, et al. PYK2 selectively mediates signals for growth versus differentiation in response to stretch of spontaneously active vascular smooth muscle. Physiol Rep. 2014;2: pubmed publisher
western blot; human	De Santis R, Rosi A, Anastasi A, Chiapparino C, Albertoni C, Leoni B, et al. Efficacy of aerosol therapy of lung cancer correlates with EGFR paralysis induced by AvidinOX-anchored biotinylated Cetuximab. Oncotarget. 2014;5:9239-55 pubmed
western blot; human; 1:1000	Jordan N, Dutkowski C, Barrow D, Mottram H, Hutcheson I, Nicholson R, et al. Impact of dual mTORC1/2 mTOR kinase inhibitor AZD8055 on acquired endocrine resistance in breast cancer in vitro. Breast Cancer Res. 2014;16:R12 pubmed publisher
western blot; human	Modi H, Cornu M, Thorens B. Glutamine stimulates biosynthesis and secretion of insulin-like growth factor 2 (IGF2), an autocrine regulator of beta cell mass and function. J Biol Chem. 2014;289:31972-82 pubmed publisher
western blot; human	Martin V, Corso S, Comoglio P, Giordano S. Increase of MET gene copy number confers resistance to a monovalent MET antibody and establishes drug dependence. Mol Oncol. 2014;8:1561-74 pubmed publisher
western blot; human	Feng C, Zhang Y, Yin J, Li J, Abounader R, Zuo Z. Regulatory factor X1 is a new tumor suppressive transcription factor that acts via direct downregulation of CD44 in glioblastoma. Neuro Oncol. 2014;16:1078-85 pubmed publisher
western blot; human	Hong Y, Kim J, Pectasides E, Fox C, Hong S, Ma Q, et al. Src mutation induces acquired lapatinib resistance in ERBB2-amplified human gastroesophageal adenocarcinoma models. PLoS ONE. 2014;9:e109440 pubmed publisher
western blot; human	Hu J, Cheng Y, Li Y, Jin Z, Pan Y, Liu G, et al. microRNA-128 plays a critical role in human non-small cell lung cancer tumourigenesis, angiogenesis and lymphangiogenesis by directly targeting vascular endothelial growth factor-C. Eur J Cancer. 2014;50:2336-50 pubmed publisher
western blot; human	Oliveira C, de Bock C, Molloy T, Sadeqzadeh E, Geng X, Hersey P, et al. Macrophage migration inhibitory factor engages PI3K/Akt signalling and is a prognostic factor in metastatic melanoma. BMC Cancer. 2014;14:630 pubmed publisher
western blot; human; 1:1000	Jo D, Kim J, Son J, Song N, Kim Y, Yu Y, et al. Anti-angiogenic effect of bare titanium dioxide nanoparticles on pathologic neovascularization without unbearable toxicity. Nanomedicine. 2014;10:1109-17 pubmed publisher
western blot; mouse; 1:1000; fig 6	Curto G, Nieto-Estévez V, Hurtado-Chong A, Valero J, Gómez C, Alonso J, et al. Pax6 is essential for the maintenance and multi-lineage differentiation of neural stem cells, and for neuronal incorporation into the adult olfactory bulb. Stem Cells Dev. 2014;23:2813-30 pubmed publisher
western blot; rat	Serizawa Y, Oshima R, Yoshida M, Sakon I, Kitani K, Goto A, et al. Salicylate acutely stimulates 5'-AMP-activated protein kinase and insulin-independent glucose transport in rat skeletal muscles. Biochem Biophys Res Commun. 2014;453:81-5 pubmed publisher
western blot; rat	Niu H, Nie L, Liu M, Chi Y, Zhang T, Li Y. Benazepril affects integrin-linked kinase and smooth muscle α-actin expression in diabetic rat glomerulus and cultured mesangial cells. BMC Nephrol. 2014;15:135 pubmed publisher
western blot; human	Ashlin T, Buckley M, Salter R, Johnson J, Kwan A, Ramji D. The anti-atherogenic cytokine interleukin-33 inhibits the expression of a disintegrin and metalloproteinase with thrombospondin motifs-1, -4 and -5 in human macrophages: Requirement of extracellular signal-regulated kinase, c-Jun N-terminal kinase and pho. Int J Biochem Cell Biol. 2014;46:113-23 pubmed publisher
western blot; mouse	Baek J, Kim J, Cheon Y, Park S, Ahn S, Yoon K, et al. Aconitum pseudo-laeve var. erectum inhibits receptor activator of nuclear factor kappa-B ligand-induced osteoclastogenesis via the c-Fos/nuclear factor of activated T-cells, cytoplasmic 1 signaling pathway and prevents lipopolysaccharide-induced bone loss. Molecules. 2014;19:11628-44 pubmed publisher
western blot; mouse	Liu X, Giguere V. Inactivation of RARβ inhibits Wnt1-induced mammary tumorigenesis by suppressing epithelial-mesenchymal transitions. Nucl Recept Signal. 2014;12:e004 pubmed publisher
western blot; human	Balko J, Giltnane J, Wang K, Schwarz L, Young C, Cook R, et al. Molecular profiling of the residual disease of triple-negative breast cancers after neoadjuvant chemotherapy identifies actionable therapeutic targets. Cancer Discov. 2014;4:232-45 pubmed publisher
western blot; human; 1:1000; fig s1	Ng Y, Lee J, Supko K, Khan A, Torres S, Berwick M, et al. Pan-erbB inhibition potentiates BRAF inhibitors for melanoma treatment. Melanoma Res. 2014;24:207-18 pubmed publisher
western blot; human	Knubel K, Pernu B, Sufit A, Nelson S, Pierce A, Keating A. MerTK inhibition is a novel therapeutic approach for glioblastoma multiforme. Oncotarget. 2014;5:1338-51 pubmed
western blot; rat	Akkad H, Corpeno R, Larsson L. Masseter muscle myofibrillar protein synthesis and degradation in an experimental critical illness myopathy model. PLoS ONE. 2014;9:e92622 pubmed publisher
western blot; mouse; 1:1000	Niu F, Yao H, Zhang W, Sutliff R, Buch S. Tat 101-mediated enhancement of brain pericyte migration involves platelet-derived growth factor subunit B homodimer: implications for human immunodeficiency virus-associated neurocognitive disorders. J Neurosci. 2014;34:11812-25 pubmed publisher
western blot; human	Wei N, Chu E, Wipf P, Schmitz J. Protein kinase d as a potential chemotherapeutic target for colorectal cancer. Mol Cancer Ther. 2014;13:1130-41 pubmed publisher
western blot; mouse; fig 3	Tan S, Shui G, Zhou J, Shi Y, Huang J, Xia D, et al. Critical role of SCD1 in autophagy regulation via lipogenesis and lipid rafts-coupled AKT-FOXO1 signaling pathway. Autophagy. 2014;10:226-42 pubmed publisher
western blot; rat	Sharma N, Sequea D, Castorena C, Arias E, Qi N, Cartee G. Heterogeneous effects of calorie restriction on in vivo glucose uptake and insulin signaling of individual rat skeletal muscles. PLoS ONE. 2014;8:e65118 pubmed publisher
western blot; mouse; fig 5, 7	Zemljic-Harpf A, Godoy J, Platoshyn O, Asfaw E, Busija A, Domenighetti A, et al. Vinculin directly binds zonula occludens-1 and is essential for stabilizing connexin-43-containing gap junctions in cardiac myocytes. J Cell Sci. 2014;127:1104-16 pubmed publisher
western blot; mouse; 1:1000	Ramos A, Rodríguez-Seoane C, Rosa I, Trossbach S, Ortega-Alonso A, Tomppo L, et al. Neuropeptide precursor VGF is genetically associated with social anhedonia and underrepresented in the brain of major mental illness: its downregulation by DISC1. Hum Mol Genet. 2014;23:5859-65 pubmed publisher
western blot; human; 1:1500; fig 1	Yu P, Laird A, Du X, Wu J, Won K, Yamaguchi K, et al. Characterization of the activity of the PI3K/mTOR inhibitor XL765 (SAR245409) in tumor models with diverse genetic alterations affecting the PI3K pathway. Mol Cancer Ther. 2014;13:1078-91 pubmed publisher
western blot; mouse; fig 2	Luo L, Wall A, Yeo J, Condon N, Norwood S, Schoenwaelder S, et al. Rab8a interacts directly with PI3Kγ to modulate TLR4-driven PI3K and mTOR signalling. Nat Commun. 2014;5:4407 pubmed publisher
western blot; rat; 1:1000	Ota K, Liu R, Voleti B, Maldonado-Aviles J, Duric V, Iwata M, et al. REDD1 is essential for stress-induced synaptic loss and depressive behavior. Nat Med. 2014;20:531-5 pubmed publisher
western blot; mouse; 1:1000; fig 3, 5	Wadosky K, Rodriguez J, Hite R, Min J, Walton B, Willis M. Muscle RING finger-1 attenuates IGF-I-dependent cardiomyocyte hypertrophy by inhibiting JNK signaling. Am J Physiol Endocrinol Metab. 2014;306:E723-39 pubmed publisher
western blot; human	Roh J, Huang J, Hu W, Yang X, Jennings N, Sehgal V, et al. Biologic effects of platelet-derived growth factor receptor α blockade in uterine cancer. Clin Cancer Res. 2014;20:2740-50 pubmed publisher
western blot; mouse; 1:1000	Zhang Y, Liu X, Han L, Gao X, Liu E, Wang T. Regulation of lipid and glucose homeostasis by mango tree leaf extract is mediated by AMPK and PI3K/AKT signaling pathways. Food Chem. 2013;141:2896-905 pubmed publisher
western blot; human	BENTLEY C, Jurinka S, Kljavin N, Vartanian S, Ramani S, Gonzalez L, et al. A requirement for wild-type Ras isoforms in mutant KRas-driven signalling and transformation. Biochem J. 2013;452:313-20 pubmed publisher
immunocytochemistry; human	Kaiser M, Kühnl A, Reins J, Fischer S, Ortiz-Tánchez J, Schlee C, et al. Antileukemic activity of the HSP70 inhibitor pifithrin-μ in acute leukemia. Blood Cancer J. 2011;1:e28 pubmed
flow cytometry; human; fig 3	Lamottke B, Kaiser M, Mieth M, Heider U, Gao Z, Nikolova Z, et al. The novel, orally bioavailable HSP90 inhibitor NVP-HSP990 induces cell cycle arrest and apoptosis in multiple myeloma cells and acts synergistically with melphalan by increased cleavage of caspases. Eur J Haematol. 2012;88:406-15 pubmed publisher
	Kim R, Cui Y, Yoo K, Kim I, Lee M, Choi Y, et al. Radiation promotes malignant phenotypes through SRC in breast cancer cells. Cancer Sci. 2015;106:78-85 pubmed publisher
	Chan C, Fang C, Qiao Y, Yarilina A, Prinjha R, Ivashkiv L. BET bromodomain inhibition suppresses transcriptional responses to cytokine-Jak-STAT signaling in a gene-specific manner in human monocytes. Eur J Immunol. 2015;45:287-97 pubmed publisher
	Singh S, Chand H, Gundavarapu S, Saeed A, Langley R, Tesfaigzi Y, et al. HIF-1α Plays a Critical Role in the Gestational Sidestream Smoke-Induced Bronchopulmonary Dysplasia in Mice. PLoS ONE. 2015;10:e0137757 pubmed publisher
	Rider C, Shah S, Miller-Larsson A, Giembycz M, Newton R. Cytokine-induced loss of glucocorticoid function: effect of kinase inhibitors, long-acting β(2)-adrenoceptor [corrected] agonist and glucocorticoid receptor ligands. PLoS ONE. 2015;10:e0116773 pubmed publisher
	Calegari-Silva T, Vivarini Á, Miqueline M, Dos Santos G, Teixeira K, Saliba A, et al. The human parasite Leishmania amazonensis downregulates iNOS expression via NF-κB p50/p50 homodimer: role of the PI3K/Akt pathway. Open Biol. 2015;5:150118 pubmed publisher
	Huber R, Lucas J, Gomez-Sarosi L, Coleman I, Zhao S, Coleman R, et al. DNA damage induces GDNF secretion in the tumor microenvironment with paracrine effects promoting prostate cancer treatment resistance. Oncotarget. 2015;6:2134-47 pubmed
	Wang M, Li F, Shi Z, Liu Y, Wang X, Li L, et al. N-cadherin is a novel ERα anchor that protects against 6-OHDA damage to dopaminergic cells. Cell Mol Neurobiol. 2014;34:123-31 pubmed publisher
	Maurin H, Lechat B, Borghgraef P, Devijver H, Jaworski T, Van Leuven F. Terminal hypothermic Tau.P301L mice have increased Tau phosphorylation independently of glycogen synthase kinase 3α/β. Eur J Neurosci. 2014;40:2442-53 pubmed publisher
	Bernardo B, Nguyen S, Winbanks C, Gao X, Boey E, Tham Y, et al. Therapeutic silencing of miR-652 restores heart function and attenuates adverse remodeling in a setting of established pathological hypertrophy. FASEB J. 2014;28:5097-110 pubmed publisher
	Chen Y, Wei H, Liu F, Guan J. Hyperactivation of mammalian target of rapamycin complex 1 (mTORC1) promotes breast cancer progression through enhancing glucose starvation-induced autophagy and Akt signaling. J Biol Chem. 2014;289:1164-73 pubmed publisher
	Denoyelle C, Lambert B, Meryet-Figuiere M, Vigneron N, Brotin E, Lecerf C, et al. miR-491-5p-induced apoptosis in ovarian carcinoma depends on the direct inhibition of both BCL-XL and EGFR leading to BIM activation. Cell Death Dis. 2014;5:e1445 pubmed publisher
	Lunardi A, Webster K, Papa A, Padmani B, Clohessy J, Bronson R, et al. Role of aberrant PI3K pathway activation in gallbladder tumorigenesis. Oncotarget. 2014;5:894-900 pubmed
	Correa R, Valdes Y, Peart T, Fazio E, Bertrand M, McGee J, et al. Combination of AKT inhibition with autophagy blockade effectively reduces ascites-derived ovarian cancer cell viability. Carcinogenesis. 2014;35:1951-61 pubmed publisher
	O'TOOLE T, Abplanalp W, Li X, Cooper N, CONKLIN D, Haberzettl P, et al. Acrolein decreases endothelial cell migration and insulin sensitivity through induction of let-7a. Toxicol Sci. 2014;140:271-82 pubmed publisher
	Masiello M, Cucina A, Proietti S, Palombo A, Coluccia P, D'Anselmi F, et al. Phenotypic switch induced by simulated microgravity on MDA-MB-231 breast cancer cells. Biomed Res Int. 2014;2014:652434 pubmed publisher
	Li D, Zhao L, Zheng X, Lin P, Lin F, Li Y, et al. Sox2 is involved in paclitaxel resistance of the prostate cancer cell line PC-3 via the PI3K/Akt pathway. Mol Med Rep. 2014;10:3169-76 pubmed publisher
	Banin R, Hirata B, Andrade I, Zemdegs J, Clemente A, Dornellas A, et al. Beneficial effects of Ginkgo biloba extract on insulin signaling cascade, dyslipidemia, and body adiposity of diet-induced obese rats. Braz J Med Biol Res. 2014;47:780-8 pubmed
	Kondratskyi A, Yassine M, Slomianny C, Kondratska K, Gordienko D, Dewailly E, et al. Identification of ML-9 as a lysosomotropic agent targeting autophagy and cell death. Cell Death Dis. 2014;5:e1193 pubmed publisher
	Zhu Y, Xu L, Fan Y, Li C, Zhang Y, Zheng H, et al. Interferon-α enhances 5'-deoxy-5-fluorouridine-induced apoptosis by ERK-dependant upregulation of thymidine phosphorylase. Biomed Res Int. 2013;2013:132793 pubmed publisher
	Zhang H, Yea K, Xie J, Ruiz D, Wilson I, Lerner R. Selecting agonists from single cells infected with combinatorial antibody libraries. Chem Biol. 2013;20:734-41 pubmed publisher
	Hussain R, Aronsen J, Afzal F, Sjaastad I, Osnes J, Skomedal T, et al. The functional activity of inhibitory G protein (G(i)) is not increased in failing heart ventricle. J Mol Cell Cardiol. 2013;56:129-38 pubmed publisher
	Zhang J, Zhang Z, Zhang D, Zhu J, Zhang T, Wang C. microRNA 126 inhibits the transition of endothelial progenitor cells to mesenchymal cells via the PIK3R2-PI3K/Akt signalling pathway. PLoS ONE. 2013;8:e83294 pubmed publisher
	Griffin M, Zhou Y, Kang S, Zhang X, Mikkelsen T, Rosen E. Early B-cell factor-1 (EBF1) is a key regulator of metabolic and inflammatory signaling pathways in mature adipocytes. J Biol Chem. 2013;288:35925-39 pubmed publisher
	Zhang W, Wang Q, Song P, Zou M. Liver kinase b1 is required for white adipose tissue growth and differentiation. Diabetes. 2013;62:2347-58 pubmed publisher
	Sadacca L, Bruno J, Wen J, Xiong W, McGraw T. Specialized sorting of GLUT4 and its recruitment to the cell surface are independently regulated by distinct Rabs. Mol Biol Cell. 2013;24:2544-57 pubmed publisher
	Lanuza-Masdeu J, Arévalo M, Vila C, Barbera A, Gomis R, Caelles C. In vivo JNK activation in pancreatic β-cells leads to glucose intolerance caused by insulin resistance in pancreas. Diabetes. 2013;62:2308-17 pubmed publisher
	Li Z, Rana T. A kinase inhibitor screen identifies small-molecule enhancers of reprogramming and iPS cell generation. Nat Commun. 2012;3:1085 pubmed publisher
	Matsuura S, Yan M, Lo M, Ahn E, Weng S, Dangoor D, et al. Negative effects of GM-CSF signaling in a murine model of t(8;21)-induced leukemia. Blood. 2012;119:3155-63 pubmed publisher
	Sykes S, Lane S, Bullinger L, Kalaitzidis D, Yusuf R, Saez B, et al. AKT/FOXO signaling enforces reversible differentiation blockade in myeloid leukemias. Cell. 2011;146:697-708 pubmed publisher
	Chung Y, Kim H, Kim I, Yim M, Chock P. Dual function of protein kinase C (PKC) in 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced manganese superoxide dismutase (MnSOD) expression: activation of CREB and FOXO3a by PKC-alpha phosphorylation and by PKC-mediated inactivation of Akt, respective. J Biol Chem. 2011;286:29681-90 pubmed publisher
	Meyer R, D'Alessandro L, Kar S, Kramer B, She B, Kaschek D, et al. Heterogeneous kinetics of AKT signaling in individual cells are accounted for by variable protein concentration. Front Physiol. 2012;3:451 pubmed publisher
	Xie Y, Pan Y, Chang C, Tang P, Huang Y, Walzem R, et al. Palmitic acid in chicken granulosa cell death-lipotoxic mechanisms mediate reproductive inefficacy of broiler breeder hens. Theriogenology. 2012;78:1917-28 pubmed publisher
	Baudy A, Dogan T, Flores-Mercado J, Hoeflich K, Su F, van Bruggen N, et al. FDG-PET is a good biomarker of both early response and acquired resistance in BRAFV600 mutant melanomas treated with vemurafenib and the MEK inhibitor GDC-0973. EJNMMI Res. 2012;2:22 pubmed
	Rohani M, DiJulio D, An J, Hacker B, Dale B, Chung W. PAR1- and PAR2-induced innate immune markers are negatively regulated by PI3K/Akt signaling pathway in oral keratinocytes. BMC Immunol. 2010;11:53 pubmed publisher

product information

SKU :

9272S

Product-Name :

Akt Antibody

Size :

100 ul

Price-(USD) :

235 USD

Species-x-Reactivity :

H, M, R, Hm, Mk, C, Dm, B, Pg, GP, (Dg)

Applications :

Flow cytometry

Product-Category :

PI3K / Akt Signaling

Shipping-Temp :

AMBIENT

Storage-Temp :

-20°C

Product-Type :

Polyclonal Antibody

MW :

Host :

Rabbit

Target :

Akt1/Akt2/Akt3

more info or order :

Cell Signaling Technology product webpage