Phospho-Akt (Ser473) Antibody | Cell Signaling Technology 9271L product information

product summary

company name :

Cell Signaling Technology

product type :

antibody

product name :

Phospho-Akt (Ser473) Antibody

catalog :

9271L

quantity :

300 ul

price :

658 USD

clonality :

polyclonal

host :

rabbit

antigen modification :

phosphorylated

reactivity :

hamsters, , gilthead bream, human, mouse, rat, dog, cow

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section, other

more info or order :

Cell Signaling Technology product webpage

citations: 187

Published Application/Species/Dilution	Reference
western blot; human; fig 1	Yoshida T, Song L, Bai Y, Kinose F, Li J, Ohaegbulam K, et al. ZEB1 Mediates Acquired Resistance to the Epidermal Growth Factor Receptor-Tyrosine Kinase Inhibitors in Non-Small Cell Lung Cancer. PLoS ONE. 2016;11:e0147344 pubmed publisher
western blot; human; 1:1000; fig 6	Verbrugge S, Al M, Assaraf Y, Kammerer S, Chandrupatla D, Honeywell R, et al. Multifactorial resistance to aminopeptidase inhibitor prodrug CHR2863 in myeloid leukemia cells: down-regulation of carboxylesterase 1, drug sequestration in lipid droplets and pro-survival activation ERK/Akt/mTOR. Oncotarget. 2016;7:5240-57 pubmed publisher
western blot; mouse; fig 5c	Katlinskaya Y, Katlinski K, Yu Q, Ortiz A, Beiting D, Brice A, et al. Suppression of Type I Interferon Signaling Overcomes Oncogene-Induced Senescence and Mediates Melanoma Development and Progression. Cell Rep. 2016;15:171-80 pubmed publisher
western blot; mouse; fig 6g	Lee Y, Yun M, Kim H, Jeon B, Park B, Lee B, et al. Exogenous administration of DLK1 ameliorates hepatic steatosis and regulates gluconeogenesis via activation of AMPK. Int J Obes (Lond). 2016;40:356-65 pubmed publisher
western blot; human; 1:1000; tbl 1	Jansson D, Scotter E, Rustenhoven J, Coppieters N, Smyth L, Oldfield R, et al. Interferon-γ blocks signalling through PDGFRβ in human brain pericytes. J Neuroinflammation. 2016;13:249 pubmed
western blot; human; 1:1000; fig 1	Carbonneau M, M Gagné L, Lalonde M, Germain M, Motorina A, Guiot M, et al. The oncometabolite 2-hydroxyglutarate activates the mTOR signalling pathway. Nat Commun. 2016;7:12700 pubmed publisher
western blot; human; 1:1000; fig s2	Deblois G, Smith H, Tam I, Gravel S, Caron M, Savage P, et al. ERRα mediates metabolic adaptations driving lapatinib resistance in breast cancer. Nat Commun. 2016;7:12156 pubmed publisher
western blot; human; fig 5	Zeng W, Liu Q, Chen Z, Wu X, Zhong Y, Wu J. Silencing of hERG1 Gene Inhibits Proliferation and Invasion, and Induces Apoptosis in Human Osteosarcoma Cells by Targeting the NF-κB Pathway. J Cancer. 2016;7:746-57 pubmed publisher
western blot; human; 1:1000; fig 2	Cipriani G, Gibbons S, Verhulst P, Choi K, Eisenman S, Hein S, et al. Diabetic Csf1(op/op) mice lacking macrophages are protected against the development of delayed gastric emptying. Cell Mol Gastroenterol Hepatol. 2016;2:40-47 pubmed
western blot; mouse; fig 4	Jang C, Oh S, Wada S, Rowe G, Liu L, Chan M, et al. A branched-chain amino acid metabolite drives vascular fatty acid transport and causes insulin resistance. Nat Med. 2016;22:421-6 pubmed publisher
western blot; human; 1:500; fig 6	Kohler T, Scholz A, Kiachludis D, Hammerschmidt S. Induction of Central Host Signaling Kinases during Pneumococcal Infection of Human THP-1 Cells. Front Cell Infect Microbiol. 2016;6:48 pubmed publisher
western blot; human; 1:1000; fig 3	Gandin V, Masvidal L, Cargnello M, Gyenis L, McLaughlan S, Cai Y, et al. mTORC1 and CK2 coordinate ternary and eIF4F complex assembly. Nat Commun. 2016;7:11127 pubmed publisher
western blot; human; 1:1000; fig 6	Song M, Wang Y, Shang Z, Liu X, Xie D, Wang Q, et al. Bystander autophagy mediated by radiation-induced exosomal miR-7-5p in non-targeted human bronchial epithelial cells. Sci Rep. 2016;6:30165 pubmed publisher
western blot; human; fig 7	Pivonello C, Negri M, De Martino M, Napolitano M, De Angelis C, Provvisiero D, et al. The dual targeting of insulin and insulin-like growth factor 1 receptor enhances the mTOR inhibitor-mediated antitumor efficacy in hepatocellular carcinoma. Oncotarget. 2016;7:9718-31 pubmed publisher
western blot; rat; 1:1000; fig 6	Hu Y, Zhang Y, Tian K, Xun C, Wang S, Lv D. Effects of nerve growth factor and basic fibroblast growth factor dual gene modification on rat bone marrow mesenchymal stem cell differentiation into neuron-like cells in vitro. Mol Med Rep. 2016;13:49-58 pubmed publisher
western blot; human; fig 5	Afsar T, Trembley J, Salomon C, Razak S, Khan M, Ahmed K. Growth inhibition and apoptosis in cancer cells induced by polyphenolic compounds of Acacia hydaspica: Involvement of multiple signal transduction pathways. Sci Rep. 2016;6:23077 pubmed publisher
western blot; mouse; 1:1000; fig 5	Dokas J, Chadt A, Joost H, Al-Hasani H. Tbc1d1 deletion suppresses obesity in leptin-deficient mice. Int J Obes (Lond). 2016;: pubmed publisher
western blot; human; fig 1	Debruyne D, Bhatnagar N, Sharma B, Luther W, Moore N, Cheung N, et al. ALK inhibitor resistance in ALK(F1174L)-driven neuroblastoma is associated with AXL activation and induction of EMT. Oncogene. 2016;35:3681-91 pubmed publisher
western blot; mouse; fig 3	Boothe T, Lim G, Cen H, Skovsø S, Piske M, Li S, et al. Inter-domain tagging implicates caveolin-1 in insulin receptor trafficking and Erk signaling bias in pancreatic beta-cells. Mol Metab. 2016;5:366-78 pubmed publisher
western blot; rat; fig 2	Kawamoto E, Koshinaka K, Yoshimura T, Masuda H, Kawanaka K. Immobilization rapidly induces muscle insulin resistance together with the activation of MAPKs (JNK and p38) and impairment of AS160 phosphorylation. Physiol Rep. 2016;4: pubmed publisher
immunohistochemistry - paraffin section; human	Southworth T, Plumb J, Gupta V, Pearson J, Ramis I, Lehner M, et al. Anti-inflammatory potential of PI3Kδ and JAK inhibitors in asthma patients. Respir Res. 2016;17:124 pubmed
western blot; mouse; 1:1000; fig 3	Thomas A, Belaidi E, Aron-Wisnewsky J, van der Zon G, Levy P, Clement K, et al. Hypoxia-inducible factor prolyl hydroxylase 1 (PHD1) deficiency promotes hepatic steatosis and liver-specific insulin resistance in mice. Sci Rep. 2016;6:24618 pubmed publisher
western blot; mouse; fig 3	Diersch S, Wirth M, Schneeweis C, Jörs S, Geisler F, Siveke J, et al. Kras(G12D) induces EGFR-MYC cross signaling in murine primary pancreatic ductal epithelial cells. Oncogene. 2016;35:3880-6 pubmed publisher
western blot; human; 1:1000; fig s2	Wang J, Hu K, Guo J, Cheng F, Lv J, Jiang W, et al. Suppression of KRas-mutant cancer through the combined inhibition of KRAS with PLK1 and ROCK. Nat Commun. 2016;7:11363 pubmed publisher
western blot; mouse; 1:1000; fig 5	Jang H, Lee G, Selby C, Lee G, Jeon Y, Lee J, et al. SREBP1c-CRY1 signalling represses hepatic glucose production by promoting FOXO1 degradation during refeeding. Nat Commun. 2016;7:12180 pubmed publisher
western blot; mouse; fig 3	Gong Q, Hu Z, Zhang F, Cui A, Chen X, Jiang H, et al. Fibroblast growth factor 21 improves hepatic insulin sensitivity by inhibiting mammalian target of rapamycin complex 1 in mice. Hepatology. 2016;: pubmed publisher
western blot; human; fig 6	Huang C, Lee C, Lin H, Chang J. Cathepsin S attenuates endosomal EGFR signalling: A mechanical rationale for the combination of cathepsin S and EGFR tyrosine kinase inhibitors. Sci Rep. 2016;6:29256 pubmed publisher
western blot; mouse; 1:1000; tbl 1	Guillot F, Kemppainen S, Lavasseur G, Miettinen P, Laroche S, Tanila H, et al. Brain-Specific Basal and Novelty-Induced Alternations in PI3K-Akt and MAPK/ERK Signaling in a Middle-Aged AβPP/PS1 Mouse Model of Alzheimer's Disease. J Alzheimers Dis. 2016;51:1157-73 pubmed publisher
western blot; human; fig 7a	Yokdang N, Hatakeyama J, Wald J, Simion C, Tellez J, Chang D, et al. LRIG1 opposes epithelial-to-mesenchymal transition and inhibits invasion of basal-like breast cancer cells. Oncogene. 2016;35:2932-47 pubmed publisher
western blot; mouse; fig 5	van Ree J, Nam H, Jeganathan K, Kanakkanthara A, van Deursen J. Pten regulates spindle pole movement through Dlg1-mediated recruitment of Eg5 to centrosomes. Nat Cell Biol. 2016;: pubmed publisher
western blot; human; fig 3	Goulielmaki M, Koustas E, Moysidou E, Vlassi M, Sasazuki T, Shirasawa S, et al. BRAF associated autophagy exploitation: BRAF and autophagy inhibitors synergise to efficiently overcome resistance of BRAF mutant colorectal cancer cells. Oncotarget. 2016;7:9188-221 pubmed publisher
western blot; mouse; 1:1000; fig 6	Herring A, Münster Y, Akkaya T, Moghaddam S, Deinsberger K, Meyer J, et al. Kallikrein-8 inhibition attenuates Alzheimer's pathology in mice. Alzheimers Dement. 2016;: pubmed publisher
western blot; rat; 1:1000; fig 5	Jeong J, Noh M, Choi J, Lee H, Kim S. Neuroprotective and antioxidant activities of bamboo salt soy sauce against H2O2-induced oxidative stress in rat cortical neurons. Exp Ther Med. 2016;11:1201-1210 pubmed
western blot; human; fig 4	Kitatani K, Usui T, Sriraman S, Toyoshima M, Ishibashi M, Shigeta S, et al. Ceramide limits phosphatidylinositol-3-kinase C2β-controlled cell motility in ovarian cancer: potential of ceramide as a metastasis-suppressor lipid. Oncogene. 2016;35:2801-12 pubmed publisher
western blot; human; 1:1000; fig 5	Li Y, Wei Z, Dong B, Lian Z, Xu Y. Silencing of phosphoglucose isomerase/autocrine motility factor decreases U87 human glioblastoma cell migration. Int J Mol Med. 2016;37:998-1004 pubmed publisher
western blot; human; fig 5	Jeong A, Han S, Lee S, Su Park J, Lu Y, Yu S, et al. Patient derived mutation W257G of PPP2R1A enhances cancer cell migration through SRC-JNK-c-Jun pathway. Sci Rep. 2016;6:27391 pubmed publisher
western blot; human; fig 6	Huang Y, Lin C, Liao H, Liu C, Chen Y, Chiu W, et al. Cholesterol overload induces apoptosis in SH-SY5Y human neuroblastoma cells through the up regulation of flotillin-2 in the lipid raft and the activation of BDNF/Trkb signaling. Neuroscience. 2016;328:201-9 pubmed publisher
western blot; human; 1:1000; fig 3	Chang T, Chen C, Wu Y, Liu J, Kuo Y, Lee K, et al. Inflammation Promotes Expression of Stemness-Related Properties in HBV-Related Hepatocellular Carcinoma. PLoS ONE. 2016;11:e0149897 pubmed publisher
western blot; human; fig S3	Wang Z, Ma B, Li H, Xiao X, Zhou W, Liu F, et al. Protein 4.1N acts as a potential tumor suppressor linking PP1 to JNK-c-Jun pathway regulation in NSCLC. Oncotarget. 2016;7:509-23 pubmed publisher
western blot; human; 1:1000; fig 5	Jing H, Sun W, Fan J, Zhang Y, Yang J, Jia J, et al. Shikonin induces apoptosis of HaCaT cells via the mitochondrial, Erk and Akt pathways. Mol Med Rep. 2016;13:3009-16 pubmed publisher
western blot; mouse; 1:300	Zarpelon A, Rodrigues F, Lopes A, Souza G, Carvalho T, Pinto L, et al. Spinal cord oligodendrocyte-derived alarmin IL-33 mediates neuropathic pain. FASEB J. 2016;30:54-65 pubmed publisher
western blot; human; fig 6	Ardini E, Menichincheri M, Banfi P, Bosotti R, De Ponti C, Pulci R, et al. Entrectinib, a Pan-TRK, ROS1, and ALK Inhibitor with Activity in Multiple Molecularly Defined Cancer Indications. Mol Cancer Ther. 2016;15:628-39 pubmed publisher
western blot; human; fig 4	Choi H, Kim M, Choi Y, Shin Y, Cho S, Ko S. Rhus verniciflua Stokes (RVS) and butein induce apoptosis of paclitaxel-resistant SKOV-3/PAX ovarian cancer cells through inhibition of AKT phosphorylation. BMC Complement Altern Med. 2016;16:122 pubmed publisher
western blot; human	Kurppa K, Denessiouk K, Johnson M, Elenius K. Activating ERBB4 mutations in non-small cell lung cancer. Oncogene. 2016;35:1283-91 pubmed publisher
western blot; human; fig 1	Cui Y, Zhao J, Yi L, Jiang Y. microRNA-153 Targets mTORC2 Component Rictor to Inhibit Glioma Cells. PLoS ONE. 2016;11:e0156915 pubmed publisher
western blot; mouse; fig 4	Yang E, Ahn S, Lee K, Mahmood U, Kim H. Early Behavioral Abnormalities and Perinatal Alterations of PTEN/AKT Pathway in Valproic Acid Autism Model Mice. PLoS ONE. 2016;11:e0153298 pubmed publisher
western blot; human; fig 7 western blot; mouse; fig 1	Cizmecioglu O, Ni J, Xie S, Zhao J, Roberts T. Rac1-mediated membrane raft localization of PI3K/p110β is required for its activation by GPCRs or PTEN loss. elife. 2016;5: pubmed publisher
western blot; human; fig 2	Leonard S, Kinsella G, Benetti E, Findlay J. Regulating the effects of GPR21, a novel target for type 2 diabetes. Sci Rep. 2016;6:27002 pubmed publisher
immunohistochemistry - frozen section; rat; 1:25; fig 3	Phatak N, Stankowska D, Krishnamoorthy R. Bcl-2, Bcl-xL, and p-AKT are involved in neuroprotective effects of transcription factor Brn3b in an ocular hypertension rat model of glaucoma. Mol Vis. 2016;22:1048-61 pubmed
western blot; human; fig 1	Lee I, Maniar K, Lydon J, Kim J. Akt regulates progesterone receptor B-dependent transcription and angiogenesis in endometrial cancer cells. Oncogene. 2016;: pubmed publisher
western blot; human; fig 2	Christensen B, Nellemann B, Jørgensen J, Pedersen S, Jessen N. Erythropoietin does not activate erythropoietin receptor signaling or lipolytic pathways in human subcutaneous white adipose tissue in vivo. Lipids Health Dis. 2016;15:160 pubmed publisher
western blot; mouse; fig 1	Pinel A, Rigaudière J, Laillet B, Pouyet C, Malpuech-Brugère C, Prip-Buus C, et al. N-3PUFA differentially modulate palmitate-induced lipotoxicity through alterations of its metabolism in C2C12 muscle cells. Biochim Biophys Acta. 2016;1861:12-20 pubmed publisher
western blot; mouse; 1:500; fig 6	Thakur A, Nigri J, Lac S, Leca J, Bressy C, Berthezene P, et al. TAp73 loss favors Smad-independent TGF-β signaling that drives EMT in pancreatic ductal adenocarcinoma. Cell Death Differ. 2016;: pubmed publisher
western blot; human; 1:1000; fig 3	Liu C, Lin S, Hsu H, Yang S, Lin C, Yang M, et al. Suspension survival mediated by PP2A-STAT3-Col XVII determines tumour initiation and metastasis in cancer stem cells. Nat Commun. 2016;7:11798 pubmed publisher
western blot; mouse; 1:1000; fig st2	Gharib M, Tao H, Fungwe T, Hajri T. Cluster Differentiating 36 (CD36) Deficiency Attenuates Obesity-Associated Oxidative Stress in the Heart. PLoS ONE. 2016;11:e0155611 pubmed publisher
western blot; human; fig s21	Kawada M, Inoue H, Ohba S, Yoshida J, Masuda T, Yamasaki M, et al. Stromal cells positively and negatively modulate the growth of cancer cells: stimulation via the PGE2-TNFα-IL-6 pathway and inhibition via secreted GAPDH-E-cadherin interaction. PLoS ONE. 2015;10:e0119415 pubmed publisher
western blot; human; fig S2	Reinardy J, Corey D, Golzio C, Mueller S, Katsanis N, Kontos C. Phosphorylation of Threonine 794 on Tie1 by Rac1/PAK1 Reveals a Novel Angiogenesis Regulatory Pathway. PLoS ONE. 2015;10:e0139614 pubmed publisher
western blot; rat; 1:1000	Ledonne A, Nobili A, Latagliata E, Cavallucci V, Guatteo E, Puglisi-Allegra S, et al. Neuregulin 1 signalling modulates mGluR1 function in mesencephalic dopaminergic neurons. Mol Psychiatry. 2015;20:959-73 pubmed publisher
western blot; human; fig 2	Vogel C, Smit M, Maddalo G, Possik P, Sparidans R, van der Burg S, et al. Cooperative induction of apoptosis in NRAS mutant melanoma by inhibition of MEK and ROCK. Pigment Cell Melanoma Res. 2015;28:307-17 pubmed publisher
western blot; mouse; 1:1000; fig 3	Messaoudi S, He Y, Gutsol A, Wight A, Hébert R, Vilmundarson R, et al. Endothelial Gata5 transcription factor regulates blood pressure. Nat Commun. 2015;6:8835 pubmed publisher
western blot; mouse; 1:5000; fig 7	Lee E, Kim S, Cho K. Reconstituted High-Density Lipoprotein Containing Human Growth Hormone-1 Shows Potent Tissue Regeneration Activity with Enhancement of Anti-Oxidant and Anti-Atherosclerotic Activities. Rejuvenation Res. 2015;18:245-56 pubmed publisher
western blot; human; 1:1000	Zhang W, Cai J, Chen S, Zheng X, Hu S, Dong W, et al. Paclitaxel resistance in MCF-7/PTX cells is reversed by paeonol through suppression of the SET/phosphatidylinositol 3-kinase/Akt pathway. Mol Med Rep. 2015;12:1506-14 pubmed publisher
western blot; rat; 1:500	Pérez-Alvarez M, Mateos L, Alonso A, Wandosell F. Estradiol and Progesterone Administration After pMCAO Stimulates the Neurological Recovery and Reduces the Detrimental Effect of Ischemia Mainly in Hippocampus. Mol Neurobiol. 2015;52:1690-703 pubmed publisher
immunocytochemistry; human western blot; human	Balhara B, Burkart A, Topcu V, Lee Y, Cowan C, Kahn C, et al. Severe insulin resistance alters metabolism in mesenchymal progenitor cells. Endocrinology. 2015;156:2039-48 pubmed publisher
western blot; human; 1:1000; fig 4	Hoekstra E, Kodach L, Das A, Ruela-de-Sousa R, Ferreira C, Hardwick J, et al. Low molecular weight protein tyrosine phosphatase (LMWPTP) upregulation mediates malignant potential in colorectal cancer. Oncotarget. 2015;6:8300-12 pubmed
western blot; rat; fig 7c	Mir S, George N, Zahoor L, Harms R, Guinn Z, SARVETNICK N. Inhibition of autophagic turnover in β-cells by fatty acids and glucose leads to apoptotic cell death. J Biol Chem. 2015;290:6071-85 pubmed publisher
western blot; human; fig 1	Yi Y, Kang H, Bae E, Oh S, Seong Y, Bae I. β-TrCP1 degradation is a novel action mechanism of PI3K/mTOR inhibitors in triple-negative breast cancer cells. Exp Mol Med. 2015;47:e143 pubmed publisher
western blot; mouse; fig 4b	Xie C, Wei D, Zhao L, Marchetto S, Mei L, Borg J, et al. Erbin is a novel substrate of the Sag-βTrCP E3 ligase that regulates KrasG12D-induced skin tumorigenesis. J Cell Biol. 2015;209:721-37 pubmed publisher
western blot; mouse; 1:250	Vinue A, Andrés-Blasco I, Herrero-Cervera A, Piqueras L, Andres V, Burks D, et al. Ink4/Arf locus restores glucose tolerance and insulin sensitivity by reducing hepatic steatosis and inflammation in mice with impaired IRS2-dependent signalling. Biochim Biophys Acta. 2015;1852:1729-42 pubmed publisher
western blot; human; 1:1000	Carthy J, Sundqvist A, Heldin A, van Dam H, Kletsas D, Heldin C, et al. Tamoxifen Inhibits TGF-β-Mediated Activation of Myofibroblasts by Blocking Non-Smad Signaling Through ERK1/2. J Cell Physiol. 2015;230:3084-92 pubmed publisher
western blot; human; fig 1 western blot; mouse; fig 1	Zhang Z, Wu N, Lu Y, Davidson D, Colonna M, Veillette A. DNAM-1 controls NK cell activation via an ITT-like motif. J Exp Med. 2015;212:2165-82 pubmed publisher
western blot; mouse; fig 5	Zhang W, Hou J, Wang X, Jiang R, Yin Y, Ji J, et al. PTPRO-mediated autophagy prevents hepatosteatosis and tumorigenesis. Oncotarget. 2015;6:9420-33 pubmed
western blot; human; fig 4	Lohberger B, Leithner A, Stuendl N, Kaltenegger H, Kullich W, Steinecker-Frohnwieser B. Diacerein retards cell growth of chondrosarcoma cells at the G2/M cell cycle checkpoint via cyclin B1/CDK1 and CDK2 downregulation. BMC Cancer. 2015;15:891 pubmed publisher
western blot; human; fig 5	Choi H, Zhang H, Park H, Choi K, Lee H, Agrawal V, et al. Yes-associated protein regulates endothelial cell contact-mediated expression of angiopoietin-2. Nat Commun. 2015;6:6943 pubmed publisher
western blot; human	Chien P, Lin C, Hsiao L, Yang C. c-Src/Pyk2/EGFR/PI3K/Akt/CREB-activated pathway contributes to human cardiomyocyte hypertrophy: Role of COX-2 induction. Mol Cell Endocrinol. 2015;409:59-72 pubmed publisher
western blot; mouse; fig 3E	Ni Y, Nagashimada M, Zhuge F, Zhan L, Nagata N, Tsutsui A, et al. Astaxanthin prevents and reverses diet-induced insulin resistance and steatohepatitis in mice: A comparison with vitamin E. Sci Rep. 2015;5:17192 pubmed publisher
western blot; human	Khalil H, Loukili N, Regamey A, Cuesta-Marbán A, Santori E, Huber M, et al. The caspase-3-p120-RasGAP module generates a NF-κB repressor in response to cellular stress. J Cell Sci. 2015;128:3502-13 pubmed publisher
western blot; mouse; 1:5000	Kim S, Ebbert K, Cordeiro M, Romero M, Zhu J, Serna V, et al. Cell autonomous phosphoinositide 3-kinase activation in oocytes disrupts normal ovarian function through promoting survival and overgrowth of ovarian follicles. Endocrinology. 2015;156:1464-76 pubmed publisher
western blot; human	Morancho B, Martinez-Barriocanal A, Villanueva J, Arribas J. Role of ADAM17 in the non-cell autonomous effects of oncogene-induced senescence. Breast Cancer Res. 2015;17:106 pubmed publisher
western blot; mouse	Quan C, Xie B, Wang H, Chen S. PKB-Mediated Thr649 Phosphorylation of AS160/TBC1D4 Regulates the R-Wave Amplitude in the Heart. PLoS ONE. 2015;10:e0124491 pubmed publisher
western blot; human; 0.5 ug/ml; fig 4b	McKee C, Sigala B, Soeda J, Mouralidarane A, Morgan M, Mazzoccoli G, et al. Amphiregulin activates human hepatic stellate cells and is upregulated in non alcoholic steatohepatitis. Sci Rep. 2015;5:8812 pubmed publisher
immunocytochemistry; human western blot; rat	Zhang L, Wang H, Ding K, Xu J. FTY720 induces autophagy-related apoptosis and necroptosis in human glioblastoma cells. Toxicol Lett. 2015;236:43-59 pubmed publisher
western blot; human; fig 5	Heinemann A, Cullinane C, De Paoli-Iseppi R, Wilmott J, Gunatilake D, Madore J, et al. Combining BET and HDAC inhibitors synergistically induces apoptosis of melanoma and suppresses AKT and YAP signaling. Oncotarget. 2015;6:21507-21 pubmed
western blot; human	Peres J, Mowla S, Prince S. The T-box transcription factor, TBX3, is a key substrate of AKT3 in melanomagenesis. Oncotarget. 2015;6:1821-33 pubmed
western blot; mouse	Alvaro-Bartolomé M, Garcia-Sevilla J. The neuroplastic index p-FADD/FADD and phosphoprotein PEA-15, interacting at GABAA receptor, are upregulated in brain cortex during midazolam-induced hypnosis in mice. Eur Neuropsychopharmacol. 2015;25:2131-44 pubmed publisher
western blot; rat	Park B, Cha S, Han B, Kim S. Angiotensin IV stimulates high atrial stretch-induced ANP secretion via insulin regulated aminopeptidase. Peptides. 2015;63:30-7 pubmed publisher
western blot; mouse immunohistochemistry; mouse	Tan X, Xue Y, Ma T, Wang X, Li J, Lan L, et al. Partial eNOS deficiency causes spontaneous thrombotic cerebral infarction, amyloid angiopathy and cognitive impairment. Mol Neurodegener. 2015;10:24 pubmed publisher
western blot; human	Roffe M, Lupinacci F, Soares L, Hajj G, Martins V. Two widely used RSK inhibitors, BI-D1870 and SL0101, alter mTORC1 signaling in a RSK-independent manner. Cell Signal. 2015;27:1630-42 pubmed publisher
western blot; human; 1:1000	Zeng Z, Jing D, Zhang X, Duan Y, Xue F. Cyclic mechanical stretch promotes energy metabolism in osteoblast-like cells through an mTOR signaling-associated mechanism. Int J Mol Med. 2015;36:947-56 pubmed publisher
western blot; mouse	Tancioni I, Miller N, Uryu S, Lawson C, Jean C, Chen X, et al. FAK activity protects nucleostemin in facilitating breast cancer spheroid and tumor growth. Breast Cancer Res. 2015;17:47 pubmed publisher
western blot; mouse; fig 5	Giampietro C, Disanza A, Bravi L, Barrios-Rodiles M, Corada M, Frittoli E, et al. The actin-binding protein EPS8 binds VE-cadherin and modulates YAP localization and signaling. J Cell Biol. 2015;211:1177-92 pubmed publisher
western blot; mouse	Azimzadeh O, Sievert W, Sarioglu H, Merl-Pham J, Yentrapalli R, Bakshi M, et al. Integrative proteomics and targeted transcriptomics analyses in cardiac endothelial cells unravel mechanisms of long-term radiation-induced vascular dysfunction. J Proteome Res. 2015;14:1203-19 pubmed publisher
other; mouse; 1:1000; fig s1	Chiang C, Uzoma I, Lane D, Memisevic V, Alem F, Yao K, et al. A reverse-phase protein microarray-based screen identifies host signaling dynamics upon Burkholderia spp. infection. Front Microbiol. 2015;6:683 pubmed publisher
western blot; human; fig 1	Chiang K, Chen H, Hsu S, Pang J, Wang S, Hsu J, et al. PTEN insufficiency modulates ER+ breast cancer cell cycle progression and increases cell growth in vitro and in vivo. Drug Des Devel Ther. 2015;9:4631-8 pubmed publisher
western blot; human; fig 8	Brohée L, Demine S, Willems J, Arnould T, Colige A, Deroanne C. Lipin-1 regulates cancer cell phenotype and is a potential target to potentiate rapamycin treatment. Oncotarget. 2015;6:11264-80 pubmed
western blot; human	Lin C, Pan C, Wang C, Liu S, Hsiao L, Yang C. Tumor necrosis factor-alpha induces VCAM-1-mediated inflammation via c-Src-dependent transactivation of EGF receptors in human cardiac fibroblasts. J Biomed Sci. 2015;22:53 pubmed publisher
western blot; human; 1:1000; fig 4b	Sadowski S, Boufraqech M, Zhang L, Mehta A, Kapur P, Zhang Y, et al. Torin2 targets dysregulated pathways in anaplastic thyroid cancer and inhibits tumor growth and metastasis. Oncotarget. 2015;6:18038-49 pubmed
western blot; mouse	Lin T, Shih Y, Chen S, Lien C, Chang C, Huang T, et al. Running exercise delays neurodegeneration in amygdala and hippocampus of Alzheimer's disease (APP/PS1) transgenic mice. Neurobiol Learn Mem. 2015;118:189-97 pubmed publisher
western blot; human; fig 2	Moody S, Schinzel A, Singh S, Izzo F, Strickland M, Luo L, et al. PRKACA mediates resistance to HER2-targeted therapy in breast cancer cells and restores anti-apoptotic signaling. Oncogene. 2015;34:2061-71 pubmed publisher
western blot; rat; fig 5	Goto A, Egawa T, Sakon I, Oshima R, Ito K, Serizawa Y, et al. Heat stress acutely activates insulin-independent glucose transport and 5'-AMP-activated protein kinase prior to an increase in HSP72 protein in rat skeletal muscle. Physiol Rep. 2015;3: pubmed publisher
immunohistochemistry - free floating section; rat	Gorojod R, Alaimo A, Porte Alcon S, Pomilio C, Saravia F, Kotler M. The autophagic- lysosomal pathway determines the fate of glial cells under manganese- induced oxidative stress conditions. Free Radic Biol Med. 2015;87:237-51 pubmed publisher
western blot; human; fig 2	Xia H, Najafov A, Geng J, Galan-Acosta L, Han X, Guo Y, et al. Degradation of HK2 by chaperone-mediated autophagy promotes metabolic catastrophe and cell death. J Cell Biol. 2015;210:705-16 pubmed publisher
western blot; human; 1:2000; fig 1c western blot; mouse; fig 1b	Lee M, Jeong M, Lee H, Han H, Ko A, Hewitt S, et al. PI3K/AKT activation induces PTEN ubiquitination and destabilization accelerating tumourigenesis. Nat Commun. 2015;6:7769 pubmed publisher
western blot; human; fig 3 western blot; mouse; fig 2	Xu Y, Morse L, da Silva R, Wang D, Battaglino R. A short report: PAMM, a novel antioxidant protein, induced by oxidative stress. Redox Biol. 2015;6:446-53 pubmed publisher
western blot; mouse; 1:1000	Cheung C, Bendris N, Paul C, Hamieh A, Anouar Y, Hahne M, et al. Cyclin A2 modulates EMT via β-catenin and phospholipase C pathways. Carcinogenesis. 2015;36:914-24 pubmed publisher
western blot; human; fig 4b	Mizuno S, Hanamura I, Ota A, Karnan S, Narita T, Ri M, et al. Overexpression of salivary-type amylase reduces the sensitivity to bortezomib in multiple myeloma cells. Int J Hematol. 2015;102:569-78 pubmed publisher
western blot; mouse; 1:1000; fig s6	Sathyamurthy A, Yin D, Barik A, Shen C, Bean J, Figueiredo D, et al. ERBB3-mediated regulation of Bergmann glia proliferation in cerebellar lamination. Development. 2015;142:522-32 pubmed publisher
western blot; human	Gorantla S, Zirlik K, Reiter A, Yu C, Illert A, von Bubnoff N, et al. F604S exchange in FIP1L1-PDGFRA enhances FIP1L1-PDGFRA protein stability via SHP-2 and SRC: a novel mode of kinase inhibitor resistance. Leukemia. 2015;29:1763-70 pubmed publisher
western blot; human; 1:500	Barrichon M, Hadi T, Wendremaire M, Ptasinski C, Seigneuric R, Marcion G, et al. Dose-dependent biphasic leptin-induced proliferation is caused by non-specific IL-6/NF-κB pathway activation in human myometrial cells. Br J Pharmacol. 2015;172:2974-90 pubmed publisher
western blot; mouse	Shaw A, Pickup M, Chytil A, Aakre M, Owens P, Moses H, et al. TGFβ signaling in myeloid cells regulates mammary carcinoma cell invasion through fibroblast interactions. PLoS ONE. 2015;10:e0117908 pubmed publisher
western blot; mouse; 1:1000	Israeli-Rosenberg S, Chen C, Li R, Deussen D, Niesman I, Okada H, et al. Caveolin modulates integrin function and mechanical activation in the cardiomyocyte. FASEB J. 2015;29:374-84 pubmed publisher
western blot; mouse; fig 4b	Patel A, Yamashita N, Ascano M, Bodmer D, Boehm E, Bodkin-Clarke C, et al. RCAN1 links impaired neurotrophin trafficking to aberrant development of the sympathetic nervous system in Down syndrome. Nat Commun. 2015;6:10119 pubmed publisher
western blot; human; fig 1	Murata Y, Uehara Y, Hosoi Y. Activation of mTORC1 under nutrient starvation conditions increases cellular radiosensitivity in human liver cancer cell lines, HepG2 and HuH6. Biochem Biophys Res Commun. 2015;468:684-90 pubmed publisher
western blot; human	Jang D, Kwon H, Jeong K, Lee J, Pak Y. Essential role of flotillin-1 palmitoylation in the intracellular localization and signaling function of IGF-1 receptor. J Cell Sci. 2015;128:2179-90 pubmed publisher
western blot; human; fig 6	Albers P, Bojsen-Møller K, Dirksen C, Serup A, Kristensen D, Frystyk J, et al. Enhanced insulin signaling in human skeletal muscle and adipose tissue following gastric bypass surgery. Am J Physiol Regul Integr Comp Physiol. 2015;309:R510-24 pubmed publisher
western blot; human; fig 2	Yeom C, Kim D, Park M, Choi J, Jeong J, Wi A, et al. Insulin-induced CARM1 upregulation facilitates hepatocyte proliferation. Biochem Biophys Res Commun. 2015;461:568-74 pubmed publisher
western blot; human; fig 6c	Barbachano A, Fernández-Barral A, Pereira F, Segura M, Ordonez-Moran P, Carrillo-de Santa Pau E, et al. SPROUTY-2 represses the epithelial phenotype of colon carcinoma cells via upregulation of ZEB1 mediated by ETS1 and miR-200/miR-150. Oncogene. 2015;: pubmed publisher
western blot; mouse; fig 6	Haim Y, Bluher M, Slutsky N, Goldstein N, Kloting N, Harman-Boehm I, et al. Elevated autophagy gene expression in adipose tissue of obese humans: A potential non-cell-cycle-dependent function of E2F1. Autophagy. 2015;11:2074-2088 pubmed
western blot; human; fig 5	Harmeier A, Obermueller S, Meyer C, Revel F, Buchy D, Chaboz S, et al. Trace amine-associated receptor 1 activation silences GSK3β signaling of TAAR1 and D2R heteromers. Eur Neuropsychopharmacol. 2015;25:2049-61 pubmed publisher
western blot; rat; fig S3	Simard E, Söllradl T, Maltais J, Boucher J, D'Orléans-Juste P, Grandbois M. Receptor for Advanced Glycation End-Products Signaling Interferes with the Vascular Smooth Muscle Cell Contractile Phenotype and Function. PLoS ONE. 2015;10:e0128881 pubmed publisher
western blot; human	Waters A, Stewart J, Atigadda V, Mroczek-Musulman E, Muccio D, Grubbs C, et al. Preclinical Evaluation of a Novel RXR Agonist for the Treatment of Neuroblastoma. Mol Cancer Ther. 2015;14:1559-69 pubmed publisher
western blot; rat; 1:1000	Harris-White M, Ferbas K, Johnson M, Eslami P, Poteshkina A, Venkova K, et al. A cell-penetrating ester of the neural metabolite lanthionine ketimine stimulates autophagy through the mTORC1 pathway: Evidence for a mechanism of action with pharmacological implications for neurodegenerative pathologies. Neurobiol Dis. 2015;84:60-8 pubmed publisher
western blot; mouse; 1:1000	Hyde G, Taylor R, Ashton N, Borland S, Wu H, Gilmore A, et al. Axl tyrosine kinase protects against tubulo-interstitial apoptosis and progression of renal failure in a murine model of chronic kidney disease and hyperphosphataemia. PLoS ONE. 2014;9:e102096 pubmed publisher
western blot; hamsters	Hall J, Sun J, Slade J, Kintner J, Bambino M, Whittimore J, et al. Host nectin-1 is required for efficient Chlamydia trachomatis serovar E development. Front Cell Infect Microbiol. 2014;4:158 pubmed publisher
western blot; mouse; 1:1000; fig 6	Curto G, Nieto-Estévez V, Hurtado-Chong A, Valero J, Gómez C, Alonso J, et al. Pax6 is essential for the maintenance and multi-lineage differentiation of neural stem cells, and for neuronal incorporation into the adult olfactory bulb. Stem Cells Dev. 2014;23:2813-30 pubmed publisher
western blot; human	Balko J, Giltnane J, Wang K, Schwarz L, Young C, Cook R, et al. Molecular profiling of the residual disease of triple-negative breast cancers after neoadjuvant chemotherapy identifies actionable therapeutic targets. Cancer Discov. 2014;4:232-45 pubmed publisher
western blot; mouse; fig 5	Pulinilkunnil T, Kienesberger P, Nagendran J, Sharma N, Young M, Dyck J. Cardiac-specific adipose triglyceride lipase overexpression protects from cardiac steatosis and dilated cardiomyopathy following diet-induced obesity. Int J Obes (Lond). 2014;38:205-15 pubmed publisher
western blot; human	Jung S, Ohk J, Jeong D, Li C, Lee S, Duan J, et al. Distinct regulatory effect of the p34SEI-1 oncoprotein on cancer metastasis in HER2/neu-positive and -negative cells. Int J Oncol. 2014;45:189-96 pubmed publisher
western blot; human; 1:1000; fig 1	Yu P, Laird A, Du X, Wu J, Won K, Yamaguchi K, et al. Characterization of the activity of the PI3K/mTOR inhibitor XL765 (SAR245409) in tumor models with diverse genetic alterations affecting the PI3K pathway. Mol Cancer Ther. 2014;13:1078-91 pubmed publisher
western blot; human	Abildgaard J, Henstridge D, Pedersen A, Langley K, Scheele C, Pedersen B, et al. In vitro palmitate treatment of myotubes from postmenopausal women leads to ceramide accumulation, inflammation and affected insulin signaling. PLoS ONE. 2014;9:e101555 pubmed publisher
western blot; human	Ashlin T, Buckley M, Salter R, Johnson J, Kwan A, Ramji D. The anti-atherogenic cytokine interleukin-33 inhibits the expression of a disintegrin and metalloproteinase with thrombospondin motifs-1, -4 and -5 in human macrophages: Requirement of extracellular signal-regulated kinase, c-Jun N-terminal kinase and pho. Int J Biochem Cell Biol. 2014;46:113-23 pubmed publisher
western blot; mouse	Yokota M, Kobayashi Y, Morita J, Suzuki H, Hashimoto Y, Sasaki Y, et al. Therapeutic effect of nanogel-based delivery of soluble FGFR2 with S252W mutation on craniosynostosis. PLoS ONE. 2014;9:e101693 pubmed publisher
western blot; mouse; fig 5, 7	Zemljic-Harpf A, Godoy J, Platoshyn O, Asfaw E, Busija A, Domenighetti A, et al. Vinculin directly binds zonula occludens-1 and is essential for stabilizing connexin-43-containing gap junctions in cardiac myocytes. J Cell Sci. 2014;127:1104-16 pubmed publisher
western blot; mouse; 1:1000	Bhaskar K, Maphis N, Xu G, Varvel N, Kokiko-Cochran O, Weick J, et al. Microglial derived tumor necrosis factor-α drives Alzheimer's disease-related neuronal cell cycle events. Neurobiol Dis. 2014;62:273-85 pubmed publisher
western blot; mouse	Sackmann-Sala L, Chiche A, Mosquera-Garrote N, Boutillon F, Cordier C, Pourmir I, et al. Prolactin-induced prostate tumorigenesis links sustained Stat5 signaling with the amplification of basal/stem cells and emergence of putative luminal progenitors. Am J Pathol. 2014;184:3105-19 pubmed publisher
western blot; rat; 1:500	Bhattachariya A, Turczynska K, Grossi M, Nordström I, Buckbinder L, Albinsson S, et al. PYK2 selectively mediates signals for growth versus differentiation in response to stretch of spontaneously active vascular smooth muscle. Physiol Rep. 2014;2: pubmed publisher
western blot; mouse	Fuller S, Richard A, Ribnicky D, Beyl R, Mynatt R, Stephens J. St. John's Wort Has Metabolically Favorable Effects on Adipocytes In Vivo. Evid Based Complement Alternat Med. 2014;2014:862575 pubmed publisher
western blot; human; 1:2000	Xiang M, Birkbak N, Vafaizadeh V, Walker S, Yeh J, Liu S, et al. STAT3 induction of miR-146b forms a feedback loop to inhibit the NF-κB to IL-6 signaling axis and STAT3-driven cancer phenotypes. Sci Signal. 2014;7:ra11 pubmed publisher
western blot; mouse; 1:5000	Chen Z, Morris D, Jiang L, Liu Y, Rui L. SH2B1 in β-cells regulates glucose metabolism by promoting β-cell survival and islet expansion. Diabetes. 2014;63:585-95 pubmed publisher
western blot; mouse; fig 2	Balbas M, Burgess M, Murali R, Wongvipat J, Skaggs B, Mundel P, et al. MAGI-2 scaffold protein is critical for kidney barrier function. Proc Natl Acad Sci U S A. 2014;111:14876-81 pubmed publisher
western blot; human; 1:1000	Hellesøy M, Blois A, Tiron C, Mannelqvist M, Akslen L, Lorens J. Akt1 activity regulates vessel maturation in a tissue engineering model of angiogenesis. Tissue Eng Part A. 2014;20:2590-603 pubmed publisher
western blot; human; 1:1000; fig 3	Lebron M, Brennan L, Damoci C, Prewett M, O'Mahony M, Duignan I, et al. A human monoclonal antibody targeting the stem cell factor receptor (c-Kit) blocks tumor cell signaling and inhibits tumor growth. Cancer Biol Ther. 2014;15:1208-18 pubmed publisher
western blot; human; fig 5	Paatero I, Seagroves T, Vaparanta K, Han W, Jones F, Johnson R, et al. Hypoxia-inducible factor-1α induces ErbB4 signaling in the differentiating mammary gland. J Biol Chem. 2014;289:22459-69 pubmed publisher
western blot; human; 1:2000	Kannike K, Sepp M, Zuccato C, Cattaneo E, Timmusk T. Forkhead transcription factor FOXO3a levels are increased in Huntington disease because of overactivated positive autofeedback loop. J Biol Chem. 2014;289:32845-57 pubmed publisher
western blot; mouse western blot; rat	Zeldich E, Chen C, Colvin T, Bove-Fenderson E, Liang J, Tucker Zhou T, et al. The neuroprotective effect of Klotho is mediated via regulation of members of the redox system. J Biol Chem. 2014;289:24700-15 pubmed publisher
western blot; rat	Mellor K, Varma U, Stapleton D, Delbridge L. Cardiomyocyte glycophagy is regulated by insulin and exposure to high extracellular glucose. Am J Physiol Heart Circ Physiol. 2014;306:H1240-5 pubmed publisher
western blot; human	Watanabe T, Nakamura S, Ono T, Ui S, Yagi S, Kagawa H, et al. Pyrrolidinium fullerene induces apoptosis by activation of procaspase-9 via suppression of Akt in primary effusion lymphoma. Biochem Biophys Res Commun. 2014;451:93-100 pubmed publisher
western blot; human	Kim S, Ahn S, Lee E, Kim S, Na K, Chae D, et al. Bilirubin activates transcription of HIF-1α in human proximal tubular cells cultured in the physiologic oxygen content. J Korean Med Sci. 2014;29:S146-54 pubmed publisher
western blot; human; 1:1000	Haas M, Onstead-Haas L, Naem E, Arnold A, Rohrbaugh N, Flowers M, et al. The effect of black seed (Nigella sativa) extract on FOXO3 expression in HepG2 cells. Phytother Res. 2014;28:873-9 pubmed publisher
western blot; mouse; 1:1000	Chen J, Long F. mTORC1 signaling controls mammalian skeletal growth through stimulation of protein synthesis. Development. 2014;141:2848-54 pubmed publisher
western blot; human	Oliveira C, de Bock C, Molloy T, Sadeqzadeh E, Geng X, Hersey P, et al. Macrophage migration inhibitory factor engages PI3K/Akt signalling and is a prognostic factor in metastatic melanoma. BMC Cancer. 2014;14:630 pubmed publisher
western blot; mouse	Taniguchi R, Fukushima H, Osawa K, Maruyama T, Yasuda H, Weih F, et al. RelB-induced expression of Cot, an MAP3K family member, rescues RANKL-induced osteoclastogenesis in alymphoplasia mice by promoting NF-κB2 processing by IKKα. J Biol Chem. 2014;289:7349-61 pubmed publisher
western blot; human; 1:1000	Jo D, Kim J, Son J, Song N, Kim Y, Yu Y, et al. Anti-angiogenic effect of bare titanium dioxide nanoparticles on pathologic neovascularization without unbearable toxicity. Nanomedicine. 2014;10:1109-17 pubmed publisher
western blot; mouse; fig 3a	Zheng X, Zhai B, Koivunen P, Shin S, Lu G, Liu J, et al. Prolyl hydroxylation by EglN2 destabilizes FOXO3a by blocking its interaction with the USP9x deubiquitinase. Genes Dev. 2014;28:1429-44 pubmed publisher
western blot; mouse	Baek J, Kim J, Cheon Y, Park S, Ahn S, Yoon K, et al. Aconitum pseudo-laeve var. erectum inhibits receptor activator of nuclear factor kappa-B ligand-induced osteoclastogenesis via the c-Fos/nuclear factor of activated T-cells, cytoplasmic 1 signaling pathway and prevents lipopolysaccharide-induced bone loss. Molecules. 2014;19:11628-44 pubmed publisher
western blot; rat	Akkad H, Corpeno R, Larsson L. Masseter muscle myofibrillar protein synthesis and degradation in an experimental critical illness myopathy model. PLoS ONE. 2014;9:e92622 pubmed publisher
western blot; human	Vanli G, Peltzer N, Dubuis G, Widmann C. The activity of the anti-apoptotic fragment generated by the caspase-3/p120 RasGAP stress-sensing module displays strict Akt isoform specificity. Cell Signal. 2014;26:2992-7 pubmed publisher
western blot; mouse; 1:300; fig 2	Moorwood C, Philippou A, Spinazzola J, Keyser B, Macarak E, Barton E. Absence of γ-sarcoglycan alters the response of p70S6 kinase to mechanical perturbation in murine skeletal muscle. Skelet Muscle. 2014;4:13 pubmed publisher
western blot; rat	Lamprecht M, Morrison B. GPR30 activation is neither necessary nor sufficient for acute neuroprotection by 17β-estradiol after an ischemic injury in organotypic hippocampal slice cultures. Brain Res. 2014;1563:131-7 pubmed publisher
western blot; mouse; fig 3	Tan S, Shui G, Zhou J, Shi Y, Huang J, Xia D, et al. Critical role of SCD1 in autophagy regulation via lipogenesis and lipid rafts-coupled AKT-FOXO1 signaling pathway. Autophagy. 2014;10:226-42 pubmed publisher
western blot; gilthead bream; 1:200	Vélez E, Lutfi E, Jiménez-Amilburu V, Riera-Codina M, Capilla E, Navarro I, et al. IGF-I and amino acids effects through TOR signaling on proliferation and differentiation of gilthead sea bream cultured myocytes. Gen Comp Endocrinol. 2014;205:296-304 pubmed publisher
western blot; human; fig 4	Kodama T, Motoi N, Ninomiya H, Sakamoto H, Kitada K, Tsukaguchi T, et al. A novel mechanism of EML4-ALK rearrangement mediated by chromothripsis in a patient-derived cell line. J Thorac Oncol. 2014;9:1638-46 pubmed publisher
western blot; mouse; 1:1000	Barns M, Gondro C, Tellam R, Radley-Crabb H, Grounds M, Shavlakadze T. Molecular analyses provide insight into mechanisms underlying sarcopenia and myofibre denervation in old skeletal muscles of mice. Int J Biochem Cell Biol. 2014;53:174-85 pubmed publisher
western blot; mouse	Tersey S, Maier B, Nishiki Y, Maganti A, Nadler J, Mirmira R. 12-lipoxygenase promotes obesity-induced oxidative stress in pancreatic islets. Mol Cell Biol. 2014;34:3735-45 pubmed publisher
western blot; rat	Serizawa Y, Oshima R, Yoshida M, Sakon I, Kitani K, Goto A, et al. Salicylate acutely stimulates 5'-AMP-activated protein kinase and insulin-independent glucose transport in rat skeletal muscles. Biochem Biophys Res Commun. 2014;453:81-5 pubmed publisher
western blot; mouse; 1:1000; fig 3, 5	Wadosky K, Rodriguez J, Hite R, Min J, Walton B, Willis M. Muscle RING finger-1 attenuates IGF-I-dependent cardiomyocyte hypertrophy by inhibiting JNK signaling. Am J Physiol Endocrinol Metab. 2014;306:E723-39 pubmed publisher
western blot; dog	Chen S, Otero Y, Mulligan K, Lundblad T, Williams P, McGuinness O. Liver, but not muscle, has an entrainable metabolic memory. PLoS ONE. 2014;9:e86164 pubmed publisher
western blot; human	Elliott S, Swift S, Busse L, Scully S, Van G, Rossi J, et al. Epo receptors are not detectable in primary human tumor tissue samples. PLoS ONE. 2013;8:e68083 pubmed publisher
western blot; human; fig 1	Riaz A, Zeller K, Johansson S. Receptor-specific mechanisms regulate phosphorylation of AKT at Ser473: role of RICTOR in β1 integrin-mediated cell survival. PLoS ONE. 2012;7:e32081 pubmed publisher
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	Fallahi-Sichani M, Moerke N, Niepel M, Zhang T, Gray N, Sorger P. Systematic analysis of BRAF(V600E) melanomas reveals a role for JNK/c-Jun pathway in adaptive resistance to drug-induced apoptosis. Mol Syst Biol. 2015;11:797 pubmed publisher
	Patel R, Liu Y, Wang B, Li R, Sebti S. Identification of novel ROCK inhibitors with anti-migratory and anti-invasive activities. Oncogene. 2014;33:550-5 pubmed publisher
	Yao K, Lau S, Ge W. Differential regulation of Kit ligand A expression in the ovary by IGF-I via different pathways. Mol Endocrinol. 2014;28:138-50 pubmed publisher
	Bernardo B, Nguyen S, Winbanks C, Gao X, Boey E, Tham Y, et al. Therapeutic silencing of miR-652 restores heart function and attenuates adverse remodeling in a setting of established pathological hypertrophy. FASEB J. 2014;28:5097-110 pubmed publisher
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	Elliott S, Busse L, Swift S, McCaffery I, Rossi J, Kassner P, et al. Lack of expression and function of erythropoietin receptors in the kidney. Nephrol Dial Transplant. 2012;27:2733-45 pubmed publisher
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	Kubota H, Noguchi R, Toyoshima Y, Ozaki Y, Uda S, Watanabe K, et al. Temporal coding of insulin action through multiplexing of the AKT pathway. Mol Cell. 2012;46:820-32 pubmed publisher
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	Fujita H, Utsumi T, Muranaka S, Ogino T, Yano H, Akiyama J, et al. Involvement of Ras/extracellular signal-regulated kinase, but not Akt pathway in risedronate-induced apoptosis of U937 cells and its suppression by cytochalasin B. Biochem Pharmacol. 2005;69:1773-84 pubmed
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	Chung Y, Kim H, Kim I, Yim M, Chock P. Dual function of protein kinase C (PKC) in 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced manganese superoxide dismutase (MnSOD) expression: activation of CREB and FOXO3a by PKC-alpha phosphorylation and by PKC-mediated inactivation of Akt, respective. J Biol Chem. 2011;286:29681-90 pubmed publisher

product information

SKU :

9271L

Product-Name :

Phospho-Akt (Ser473) Antibody

Size :

300 ul

Price-(USD) :

658 USD

Species-x-Reactivity :

H, M, R, Hm, Dm, B, Dg, Pg, (Mk, C, X, Hr)

Applications :

Flow cytometry

Product-Category :

PI3K / Akt Signaling

Shipping-Temp :

AMBIENT

Storage-Temp :

-20°C

Product-Type :

Polyclonal Antibody

MW :

Host :

Rabbit

Target :

Akt1 (Ser473) phosphate/Akt2 (Ser474) phosphate/Akt3 (Ser472) phosphate

more info or order :

Cell Signaling Technology product webpage