Phospho-IκBα (Ser32/36) (5A5) Mouse mAb | Cell Signaling Technology 9246 product information

product summary

company name :

Cell Signaling Technology

product type :

antibody

product name :

Phospho-IκBα (Ser32/36) (5A5) Mouse mAb

catalog :

9246

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

antigen modification :

phosphorylated

clone name :

5A5

reactivity :

human, mouse, rat, dogs, bovine, pigs

application :

western blot, immunohistochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, other

more info or order :

Cell Signaling Technology product webpage

citations: 132

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; fig 5i	Ma S, Xu H, Huang W, Gao Y, Zhou H, Li X, et al. Chrysophanol Relieves Cisplatin-Induced Nephrotoxicity via Concomitant Inhibition of Oxidative Stress, Apoptosis, and Inflammation. Front Physiol. 2021;12:706359 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 3c	Schünke H, Göbel U, Dikic I, Pasparakis M. OTULIN inhibits RIPK1-mediated keratinocyte necroptosis to prevent skin inflammation in mice. Nat Commun. 2021;12:5912 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 1h	Hoste E, Lecomte K, Annusver K, Vandamme N, Roels J, Maschalidi S, et al. OTULIN maintains skin homeostasis by controlling keratinocyte death and stem cell identity. Nat Commun. 2021;12:5913 pubmed publisher
western blot; human; 1:500; fig 4b	Nadeem L, Balendran R, Dorogin A, Mesiano S, Shynlova O, Lye S. Pro-inflammatory signals induce 20α-HSD expression in myometrial cells: A key mechanism for local progesterone withdrawal. J Cell Mol Med. 2021;25:6773-6785 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 1a	Xu L, Humphries F, Delagic N, Wang B, Holland A, Edgar K, et al. ECSIT is a critical limiting factor for cardiac function. JCI Insight. 2021;6: pubmed publisher
western blot; human; 1:1000; loading ...; fig 4a	Lindfors S, Polianskyte Prause Z, Bouslama R, Lehtonen E, Mannerla M, Nisen H, et al. Adiponectin receptor agonist AdipoRon ameliorates renal inflammation in diet-induced obese mice and endotoxin-treated human glomeruli ex vivo. Diabetologia. 2021;64:1866-1879 pubmed publisher
western blot; mouse; fig 2a	Watahiki A, Hoshikawa S, Chiba M, Egusa H, Fukumoto S, Inuzuka H. Deficiency of Lipin2 Results in Enhanced NF-κB Signaling and Osteoclast Formation in RAW-D Murine Macrophages. Int J Mol Sci. 2021;22: pubmed publisher
western blot; mouse; loading ...; fig s2	Brea R, Valdecantos P, Rada P, Alen R, García Monzón C, Bosca L, et al. Chronic treatment with acetaminophen protects against liver aging by targeting inflammation and oxidative stress. Aging (Albany NY). 2021;13:7800-7827 pubmed publisher
western blot; mouse; 1:500; loading ...; fig 5a	Jin X, Morro B, Tørresen O, Moiche V, Solbakken M, Jakobsen K, et al. Innovation in Nucleotide-Binding Oligomerization-Like Receptor and Toll-Like Receptor Sensing Drives the Major Histocompatibility Complex-II Free Atlantic Cod Immune System. Front Immunol. 2020;11:609456 pubmed publisher
western blot; mouse; 1:1500; loading ...; fig 5c	Wu Y, Chou T, Young L, Hsieh F, Pan H, Mo S, et al. Tumor suppressor death-associated protein kinase 1 inhibits necroptosis by p38 MAPK activation. Cell Death Dis. 2020;11:305 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 3d	Xu M, Ge C, Qin Y, Lou D, Li Q, Feng J, et al. Functional loss of inactive rhomboid-like protein 2 mitigates obesity by suppressing pro-inflammatory macrophage activation-triggered adipose inflammation. Mol Metab. 2020;34:112-123 pubmed publisher
western blot; human; fig s1a	Chen M, Zhao Z, Meng Q, Liang P, Su Z, Wu Y, et al. TRIM14 Promotes Noncanonical NF-κB Activation by Modulating p100/p52 Stability via Selective Autophagy. Adv Sci (Weinh). 2020;7:1901261 pubmed publisher
western blot; human; loading ...; fig 2a	Gehring T, Erdmann T, Rahm M, Graß C, Flatley A, O Neill T, et al. MALT1 Phosphorylation Controls Activation of T Lymphocytes and Survival of ABC-DLBCL Tumor Cells. Cell Rep. 2019;29:873-888.e10 pubmed publisher
western blot; human; 1:1000; loading ...; fig 3b	Jin Y, Li Y, Wang X, Yang Y. Secretory leukocyte protease inhibitor suppresses HPV E6-expressing HNSCC progression by mediating NF-κB and Akt pathways. Cancer Cell Int. 2019;19:220 pubmed publisher
western blot; pigs ; 1:500; loading ...; fig 3	Sun P, Sun N, Yin W, Sun Y, Fan K, Guo J, et al. Matrine inhibits IL-1β secretion in primary porcine alveolar macrophages through the MyD88/NF-κB pathway and NLRP3 inflammasome. Vet Res. 2019;50:53 pubmed publisher
western blot; human; loading ...; fig 3d	Vickman R, Yang J, Lanman N, Cresswell G, Zheng F, Zhang C, et al. Cholesterol Sulfotransferase SULT2B1b Modulates Sensitivity to Death Receptor Ligand TNFα in Castration-Resistant Prostate Cancer. Mol Cancer Res. 2019;17:1253-1263 pubmed publisher
western blot; mouse; loading ...; fig 4b	Ye P, Liu J, Xu W, Liu D, Ding X, Le S, et al. Dual-Specificity Phosphatase 26 Protects Against Nonalcoholic Fatty Liver Disease in Mice Through Transforming Growth Factor Beta-Activated Kinase 1 Suppression. Hepatology. 2019;69:1946-1964 pubmed publisher
western blot; mouse; loading ...; fig s1a	Dong S, Harrington B, Hu E, Greene J, Lehman A, Tran M, et al. PI3K p110δ inactivation antagonizes chronic lymphocytic leukemia and reverses T cell immune suppression. J Clin Invest. 2019;129:122-136 pubmed publisher
western blot; human; fig 3c	Fauster A, Rebsamen M, Willmann K, César Razquin A, Girardi E, Bigenzahn J, et al. Systematic genetic mapping of necroptosis identifies SLC39A7 as modulator of death receptor trafficking. Cell Death Differ. 2019;26:1138-1155 pubmed publisher
western blot; human; 1:1000; loading ...; fig 1e	Sheng C, Yao C, Wang Z, Chen H, Zhao Y, Xu D, et al. Cyclophilin J limits inflammation through the blockage of ubiquitin chain sensing. Nat Commun. 2018;9:4381 pubmed publisher
western blot; mouse; loading ...; fig 5a	Xu X, Xu J, Wu J, Hu Y, Han Y, Gu Y, et al. Phosphorylation-Mediated IFN-γR2 Membrane Translocation Is Required to Activate Macrophage Innate Response. Cell. 2018;175:1336-1351.e17 pubmed publisher
western blot; mouse; loading ...	Peltzer N, Darding M, Montinaro A, Dráber P, Draberova H, Kupka S, et al. LUBAC is essential for embryogenesis by preventing cell death and enabling haematopoiesis. Nature. 2018;557:112-117 pubmed publisher
western blot; mouse; loading ...; fig 6h	Xi J, Huang Q, Wang L, Ma X, Deng Q, Kumar M, et al. miR-21 depletion in macrophages promotes tumoricidal polarization and enhances PD-1 immunotherapy. Oncogene. 2018;37:3151-3165 pubmed publisher
western blot; human; 1:1000; loading ...; fig 6c	Liu F, Dai M, Xu Q, Zhu X, Zhou Y, Jiang S, et al. SRSF10-mediated IL1RAP alternative splicing regulates cervical cancer oncogenesis via mIL1RAP-NF-κB-CD47 axis. Oncogene. 2018;37:2394-2409 pubmed publisher
western blot; mouse; loading ...; fig 6a	Lv R, Zhao J, Lei M, Xiao D, Yu Y, Xie J. IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3. Cell Physiol Biochem. 2017;42:1961-1972 pubmed publisher
western blot; rat; loading ...; fig 6h	Shi Y, Zhang X, Chen C, Tang M, Wang Z, Liang X, et al. Schisantherin A attenuates ischemia/reperfusion-induced neuronal injury in rats via regulation of TLR4 and C5aR1 signaling pathways. Brain Behav Immun. 2017;66:244-256 pubmed publisher
western blot; mouse; loading ...; fig 1g	Van T, Polykratis A, Straub B, Kondylis V, Papadopoulou N, Pasparakis M. Kinase-independent functions of RIPK1 regulate hepatocyte survival and liver carcinogenesis. J Clin Invest. 2017;127:2662-2677 pubmed publisher
flow cytometry; mouse; fig 2a western blot; mouse; fig 5a	Knudson K, Pritzl C, Saxena V, Altman A, Daniels M, Teixeiro E. NFÎºB-Pim-1-Eomesodermin axis is critical for maintaining CD8 T-cell memory quality. Proc Natl Acad Sci U S A. 2017;114:E1659-E1667 pubmed publisher
western blot; mouse; loading ...; fig 7c	Xiong X, Liu Y, Mei Y, Peng J, Wang Z, Kong B, et al. Novel Protective Role of Myeloid Differentiation 1 in Pathological Cardiac Remodelling. Sci Rep. 2017;7:41857 pubmed publisher
western blot; human; loading ...; fig 1a western blot; mouse; loading ...; fig 2a	Shibata Y, Tokunaga F, Goto E, Komatsu G, Gohda J, Saeki Y, et al. HTLV-1 Tax Induces Formation of the Active Macromolecular IKK Complex by Generating Lys63- and Met1-Linked Hybrid Polyubiquitin Chains. PLoS Pathog. 2017;13:e1006162 pubmed publisher
western blot; mouse; loading ...; fig 6d	Chao M, Guo J, Cheng W, Zhu X, She Z, Huang Z, et al. Loss of Caspase-Activated DNase Protects Against Atherosclerosis in Apolipoprotein E-Deficient Mice. J Am Heart Assoc. 2016;5: pubmed publisher
western blot; human; fig 3a, b	Sasaki C, Toman J, Vageli D. The In Vitro Effect of Acidic-Pepsin on Nuclear Factor KappaB Activation and Its Related Oncogenic Effect on Normal Human Hypopharyngeal Cells. PLoS ONE. 2016;11:e0168269 pubmed publisher
western blot; mouse; fig 3	Lin J, Kumari S, Kim C, Van T, Wachsmuth L, Polykratis A, et al. RIPK1 counteracts ZBP1-mediated necroptosis to inhibit inflammation. Nature. 2016;540:124-128 pubmed publisher
western blot; pigs ; loading ...; fig 1a	Ismail H, Didangelos A, Vincent T, Saklatvala J. Rapid Activation of Transforming Growth Factor ?-Activated Kinase 1 in Chondrocytes by Phosphorylation and K63 -Linked Polyubiquitination Upon Injury to Animal Articular Cartilage. Arthritis Rheumatol. 2017;69:565-575 pubmed publisher
western blot; human; 1:1000; fig s15	Nakazawa S, Oikawa D, Ishii R, Ayaki T, Takahashi H, Takeda H, et al. Linear ubiquitination is involved in the pathogenesis of optineurin-associated amyotrophic lateral sclerosis. Nat Commun. 2016;7:12547 pubmed publisher
western blot; human; fig 5	Kupka S, De Miguel D, Dráber P, Martino L, Surinova S, Rittinger K, et al. SPATA2-Mediated Binding of CYLD to HOIP Enables CYLD Recruitment to Signaling Complexes. Cell Rep. 2016;16:2271-80 pubmed publisher
western blot; mouse; 1:2000; fig 5 western blot; human; 1:2000; fig 5	Pourcelot M, Zemirli N, Silva da Costa L, Loyant R, Garcin D, Vitour D, et al. The Golgi apparatus acts as a platform for TBK1 activation after viral RNA sensing. BMC Biol. 2016;14:69 pubmed publisher
western blot; human; 1:1000; loading ...; fig s8c	Hoare M, Ito Y, Kang T, Weekes M, Matheson N, Patten D, et al. NOTCH1 mediates a switch between two distinct secretomes during senescence. Nat Cell Biol. 2016;18:979-92 pubmed publisher
western blot; mouse; 1:1000; fig 8	Wang X, Shaw D, Hammond H, Sutterwala F, Rayamajhi M, Shirey K, et al. The Prostaglandin E2-EP3 Receptor Axis Regulates Anaplasma phagocytophilum-Mediated NLRC4 Inflammasome Activation. PLoS Pathog. 2016;12:e1005803 pubmed publisher
immunohistochemistry - paraffin section; mouse; loading ...; fig 1a	Lesina M, Wörmann S, Morton J, Diakopoulos K, Korneeva O, Wimmer M, et al. RelA regulates CXCL1/CXCR2-dependent oncogene-induced senescence in murine Kras-driven pancreatic carcinogenesis. J Clin Invest. 2016;126:2919-32 pubmed publisher
western blot; mouse; fig 4a	Lazzarini E, Balbi C, Altieri P, Pfeffer U, Gambini E, Canepa M, et al. The human amniotic fluid stem cell secretome effectively counteracts doxorubicin-induced cardiotoxicity. Sci Rep. 2016;6:29994 pubmed publisher
western blot; mouse; loading ...; fig 5a	Fujiwara T, Zhou J, Ye S, Zhao H. RNA-binding protein Musashi2 induced by RANKL is critical for osteoclast survival. Cell Death Dis. 2016;7:e2300 pubmed publisher
western blot; mouse; 1:2000; loading ...; fig 2e	Luo W, Li S, Li C, Lian H, Yang Q, Zhong B, et al. iRhom2 is essential for innate immunity to DNA viruses by mediating trafficking and stability of the adaptor STING. Nat Immunol. 2016;17:1057-66 pubmed publisher
western blot; mouse; fig 5	Wang T, Pan D, Zhou Z, You Y, Jiang C, Zhao X, et al. Dectin-3 Deficiency Promotes Colitis Development due to Impaired Antifungal Innate Immune Responses in the Gut. PLoS Pathog. 2016;12:e1005662 pubmed publisher
western blot; mouse; fig 6	Li J, Hardy K, Phetsouphanh C, Tu W, Sutcliffe E, McCuaig R, et al. Nuclear PKC-? facilitates rapid transcriptional responses in human memory CD4+ T cells through p65 and H2B phosphorylation. J Cell Sci. 2016;129:2448-61 pubmed publisher
western blot; human; loading ...; fig 7	Pereira D, Simões A, Gomes S, Castro R, Carvalho T, Rodrigues C, et al. MEK5/ERK5 signaling inhibition increases colon cancer cell sensitivity to 5-fluorouracil through a p53-dependent mechanism. Oncotarget. 2016;7:34322-40 pubmed publisher
western blot; human; loading ...; fig 4d	Afonina I, Van Nuffel E, Baudelet G, Driege Y, Kreike M, Staal J, et al. The paracaspase MALT1 mediates CARD14-induced signaling in keratinocytes. EMBO Rep. 2016;17:914-27 pubmed publisher
western blot; mouse; loading ...; fig 4a	Yu Z, Chen T, Li X, Yang M, Tang S, Zhu X, et al. Lys29-linkage of ASK1 by Skp1-Cullin 1-Fbxo21 ubiquitin ligase complex is required for antiviral innate response. elife. 2016;5: pubmed publisher
western blot; mouse; fig 4	Aaes T, Kaczmarek A, Delvaeye T, De Craene B, De Koker S, Heyndrickx L, et al. Vaccination with Necroptotic Cancer Cells Induces Efficient Anti-tumor Immunity. Cell Rep. 2016;15:274-87 pubmed publisher
western blot; human; 1:1000; fig 2	Douanne T, Gavard J, BidÃ¨re N. The paracaspase MALT1 cleaves the LUBAC subunit HOIL1 during antigen receptor signaling. J Cell Sci. 2016;129:1775-80 pubmed publisher
western blot; human; fig 5	Afsar T, Trembley J, Salomon C, Razak S, Khan M, Ahmed K. Growth inhibition and apoptosis in cancer cells induced by polyphenolic compounds of Acacia hydaspica: Involvement of multiple signal transduction pathways. Sci Rep. 2016;6:23077 pubmed publisher
western blot; mouse; fig 3	Barroso GonzÃ¡lez J, Auclair S, Luan S, Thomas L, Atkins K, Aslan J, et al. PACS-2 mediates the ATM and NF-ÎºB-dependent induction of anti-apoptotic Bcl-xL in response to DNA damage. Cell Death Differ. 2016;23:1448-57 pubmed publisher
western blot; mouse; 1:1000; fig 6	Wang P, Zhang X, Luo P, Jiang X, Zhang P, Guo J, et al. Hepatocyte TRAF3 promotes liver steatosis and systemic insulin resistance through targeting TAK1-dependent signalling. Nat Commun. 2016;7:10592 pubmed publisher
western blot; human; fig 2	Schirmer M, Trentin L, Queudeville M, Seyfried F, Demir S, Tausch E, et al. Intrinsic and chemo-sensitizing activity of SMAC-mimetics on high-risk childhood acute lymphoblastic leukemia. Cell Death Dis. 2016;7:e2052 pubmed publisher
western blot; mouse; fig 6	Chhibber Goel J, Coleman Vaughan C, Agrawal V, Sawhney N, Hickey E, Powell J, et al. Î³-Secretase Activity Is Required for Regulated Intramembrane Proteolysis of Tumor Necrosis Factor (TNF) Receptor 1 and TNF-mediated Pro-apoptotic Signaling. J Biol Chem. 2016;291:5971-85 pubmed publisher
western blot; mouse; loading ...; fig 5a	Vincendeau M, Hadian K, Messias A, Brenke J, Hålander J, Griesbach R, et al. Inhibition of Canonical NF-ÎºB Signaling by a Small Molecule Targeting NEMO-Ubiquitin Interaction. Sci Rep. 2016;6:18934 pubmed publisher
western blot; human; fig 6b	Pedersen S, Chan W, Jattani R, Mackie d, Pomerantz J. Negative Regulation of CARD11 Signaling and Lymphoma Cell Survival by the E3 Ubiquitin Ligase RNF181. Mol Cell Biol. 2015;36:794-808 pubmed publisher
western blot; human; 1:1000; fig s2d	Roth Flach R, Skoura A, Matevossian A, Danai L, Zheng W, Cortes C, et al. Endothelial protein kinase MAP4K4 promotes vascular inflammation and atherosclerosis. Nat Commun. 2015;6:8995 pubmed publisher
western blot; mouse; fig 5	Kaizuka T, Mizushima N. Atg13 Is Essential for Autophagy and Cardiac Development in Mice. Mol Cell Biol. 2016;36:585-95 pubmed publisher
western blot; human; fig 1	Yamagishi M, Katano H, Hishima T, Shimoyama T, Ota Y, Nakano K, et al. Coordinated loss of microRNA group causes defenseless signaling in malignant lymphoma. Sci Rep. 2015;5:17868 pubmed publisher
western blot; human; fig 2	Wu W, Chen Q, Geng F, Tong L, Yang R, Yang J, et al. Calcineurin B stimulates cytokine production through a CD14-independent Toll-like receptor 4 pathway. Immunol Cell Biol. 2016;94:285-92 pubmed publisher
western blot; mouse; fig 6a	Bugajev V, Hálová I, Dráberová L, Bambousková M, Potůčková L, Draberova H, et al. Negative regulatory roles of ORMDL3 in the FcÎµRI-triggered expression of proinflammatory mediators and chemotactic response in murine mast cells. Cell Mol Life Sci. 2016;73:1265-85 pubmed publisher
western blot; human; 1:1000; fig 6	Qiu H, Liu B, Liu W, Liu S. Interleukin-27 enhances TNF-Î±-mediated activation of human coronary artery endothelial cells. Mol Cell Biochem. 2016;411:1-10 pubmed publisher
western blot; human; fig 4	Suzuki M, Watanabe M, Nakamaru Y, Takagi D, Takahashi H, Fukuda S, et al. TRIM39 negatively regulates the NFÎºB-mediated signaling pathway through stabilization of Cactin. Cell Mol Life Sci. 2016;73:1085-101 pubmed publisher
western blot; mouse; fig 1	Lin D, Zhang M, Zhang M, Ren Y, Jin J, Zhao Q, et al. Induction of USP25 by viral infection promotes innate antiviral responses by mediating the stabilization of TRAF3 and TRAF6. Proc Natl Acad Sci U S A. 2015;112:11324-9 pubmed publisher
other; mouse; 1:2000; fig s1	Chiang C, Uzoma I, Lane D, MemiÅ¡eviÄ‡ V, Alem F, Yao K, et al. A reverse-phase protein microarray-based screen identifies host signaling dynamics upon Burkholderia spp. infection. Front Microbiol. 2015;6:683 pubmed publisher
western blot; mouse; 1:1000; fig s5	Nakamura R, Sene A, Santeford A, Gdoura A, Kubota S, Zapata N, et al. IL10-driven STAT3 signalling in senescent macrophages promotes pathological eye angiogenesis. Nat Commun. 2015;6:7847 pubmed publisher
western blot; mouse; fig 1	Chen I, Hsu P, Hsu W, Chen N, Tseng P. Polyubiquitination of Transforming Growth Factor Î²-activated Kinase 1 (TAK1) at Lysine 562 Residue Regulates TLR4-mediated JNK and p38 MAPK Activation. Sci Rep. 2015;5:12300 pubmed publisher
western blot; rat; 1:1000	Tang H, Hua F, Wang J, Yousuf S, Atif F, Sayeed I, et al. Progesterone and vitamin D combination therapy modulates inflammatory response after traumatic brain injury. Brain Inj. 2015;29:1165-1174 pubmed publisher
western blot; human	Fauster A, Rebsamen M, Huber K, Bigenzahn J, Stukalov A, Lardeau C, et al. A cellular screen identifies ponatinib and pazopanib as inhibitors of necroptosis. Cell Death Dis. 2015;6:e1767 pubmed publisher
western blot; human; fig s2	Pan D, Jiang C, Ma Z, Blonska M, You M, Lin X. MALT1 is required for EGFR-induced NF-?B activation and contributes to EGFR-driven lung cancer progression. Oncogene. 2016;35:919-28 pubmed publisher
immunohistochemistry - paraffin section; mouse; fig 4 western blot; mouse; fig 4	Cha J, Burnum Johnson K, Bartos A, Li Y, Baker E, Tilton S, et al. Muscle Segment Homeobox Genes Direct Embryonic Diapause by Limiting Inflammation in the Uterus. J Biol Chem. 2015;290:15337-49 pubmed publisher
western blot; rat; fig 3	Bhushan S, Tchatalbachev S, Lu Y, FrÃ¶hlich S, Fijak M, Vijayan V, et al. Differential activation of inflammatory pathways in testicular macrophages provides a rationale for their subdued inflammatory capacity. J Immunol. 2015;194:5455-64 pubmed publisher
western blot; human; fig s1	Yang Y, Kim D, Seo Y, Park D, Jang H, Choi S, et al. Elevated O-GlcNAcylation promotes colonic inflammation and tumorigenesis by modulating NF-ÎºB signaling. Oncotarget. 2015;6:12529-42 pubmed
western blot; mouse	Lee S, Kim M, Lim W, Kim T, Kang C. Strenuous exercise induces mitochondrial damage in skeletal muscle of old mice. Biochem Biophys Res Commun. 2015;461:354-60 pubmed publisher
western blot; human; fig f4	Yang L, Zhang S, George S, Teng R, You X, Xu M, et al. Targeting Notch1 and proteasome as an effective strategy to suppress T-cell lymphoproliferative neoplasms. Oncotarget. 2015;6:14953-69 pubmed
immunohistochemistry - paraffin section; mouse; fig 5	Smedlund K, Birnbaumer L, Vazquez G. Increased size and cellularity of advanced atherosclerotic lesions in mice with endothelial overexpression of the human TRPC3 channel. Proc Natl Acad Sci U S A. 2015;112:E2201-6 pubmed publisher
western blot; human; fig 1	SimÃµes A, Pereira D, Gomes S, Brito H, Carvalho T, French A, et al. Aberrant MEK5/ERK5 signalling contributes to human colon cancer progression via NF-ÎºB activation. Cell Death Dis. 2015;6:e1718 pubmed publisher
western blot; mouse	Bao M, Cai Z, Zhang X, Li L, Liu X, Wan N, et al. Dickkopf-3 protects against cardiac dysfunction and ventricular remodelling following myocardial infarction. Basic Res Cardiol. 2015;110:25 pubmed publisher
western blot; mouse; 1:1000; fig 2a western blot; human; 1:1000; loading ...; fig 2c	Dong T, Li C, Wang X, Dian L, Zhang X, Li L, et al. Ainsliadimer A selectively inhibits IKKα/β by covalently binding a conserved cysteine. Nat Commun. 2015;6:6522 pubmed publisher
western blot; mouse; 1:1000	Freeman J, Feng Y, Demehri F, Dempsey P, Teitelbaum D. TPN-associated intestinal epithelial cell atrophy is modulated by TLR4/EGF signaling pathways. FASEB J. 2015;29:2943-58 pubmed publisher
western blot; human	Ismail H, Yamamoto K, Vincent T, Nagase H, Troeberg L, Saklatvala J. Interleukin-1 Acts via the JNK-2 Signaling Pathway to Induce Aggrecan Degradation by Human Chondrocytes. Arthritis Rheumatol. 2015;67:1826-36 pubmed publisher
western blot; mouse; fig 5a	Ouchida R, Lu Q, Liu J, Li Y, Chu Y, Tsubata T, et al. FcÎ¼R interacts and cooperates with the B cell receptor To promote B cell survival. J Immunol. 2015;194:3096-101 pubmed publisher
western blot; human; fig 4	Lewis M, Vyse S, Shields A, Boeltz S, Gordon P, Spector T, et al. UBE2L3 polymorphism amplifies NF-ÎºB activation and promotes plasma cell development, linking linear ubiquitination to multiple autoimmune diseases. Am J Hum Genet. 2015;96:221-34 pubmed publisher
western blot; mouse	Heo D, Lim H, Nam J, Lee M, Kim J. Regulation of phagocytosis and cytokine secretion by store-operated calcium entry in primary isolated murine microglia. Cell Signal. 2015;27:177-86 pubmed publisher
western blot; human; 1:1000	Cañeda Guzmán I, Salaiza Suazo N, Fernández Figueroa E, Carrada Figueroa G, Aguirre García M, Becker I. NK cell activity differs between patients with localized and diffuse cutaneous leishmaniasis infected with Leishmania mexicana: a comparative study of TLRs and cytokines. PLoS ONE. 2014;9:e112410 pubmed publisher
western blot; mouse; fig 2	Wang X, Li Y, Liu S, Yu X, Li L, Shi C, et al. Direct activation of RIP3/MLKL-dependent necrosis by herpes simplex virus 1 (HSV-1) protein ICP6 triggers host antiviral defense. Proc Natl Acad Sci U S A. 2014;111:15438-43 pubmed publisher
western blot; mouse; fig s2	Takahashi N, Vereecke L, Bertrand M, Duprez L, Berger S, Divert T, et al. RIPK1 ensures intestinal homeostasis by protecting the epithelium against apoptosis. Nature. 2014;513:95-9 pubmed publisher
western blot; mouse	Huang S, Lee C, Wang H, Chang Y, Lin C, Chen C, et al. 6-Dehydrogingerdione restrains lipopolysaccharide-induced inflammatory responses in RAW 264.7 macrophages. J Agric Food Chem. 2014;62:9171-9 pubmed publisher
western blot; mouse; 1:500	Nteeba J, Ganesan S, Keating A. Progressive obesity alters ovarian folliculogenesis with impacts on pro-inflammatory and steroidogenic signaling in female mice. Biol Reprod. 2014;91:86 pubmed publisher
western blot; mouse; fig 6	Tang S, Chen T, Yu Z, Zhu X, Yang M, Xie B, et al. RasGRP3 limits Toll-like receptor-triggered inflammatory response in macrophages by activating Rap1 small GTPase. Nat Commun. 2014;5:4657 pubmed publisher
western blot; human	Tong L, Wu S. The role of constitutive nitric-oxide synthase in ultraviolet B light-induced nuclear factor ÎºB activity. J Biol Chem. 2014;289:26658-68 pubmed publisher
western blot; mouse; 1:1000	Yu B, Chang J, Liu Y, Li J, Kevork K, Al Hezaimi K, et al. Wnt4 signaling prevents skeletal aging and inflammation by inhibiting nuclear factor-?B. Nat Med. 2014;20:1009-17 pubmed publisher
western blot; human; loading ...; fig s4	Baens M, Bonsignore L, Somers R, Vanderheydt C, Weeks S, Gunnarsson J, et al. MALT1 auto-proteolysis is essential for NF-κB-dependent gene transcription in activated lymphocytes. PLoS ONE. 2014;9:e103774 pubmed publisher
western blot; mouse	Lo Sasso G, Menzies K, Mottis A, Piersigilli A, Perino A, Yamamoto H, et al. SIRT2 deficiency modulates macrophage polarization and susceptibility to experimental colitis. PLoS ONE. 2014;9:e103573 pubmed publisher
western blot; human; fig 1	Mashukova A, Kozhekbaeva Z, Forteza R, Dulam V, Figueroa Y, Warren R, et al. The BAG-1 isoform BAG-1M regulates keratin-associated Hsp70 chaperoning of aPKC in intestinal cells during activation of inflammatory signaling. J Cell Sci. 2014;127:3568-77 pubmed publisher
western blot; mouse; 1:1000	Chen R, Zhang F, Song L, Shu Y, Lin Y, Dong L, et al. Transcriptome profiling reveals that the SM22?-regulated molecular pathways contribute to vascular pathology. J Mol Cell Cardiol. 2014;72:263-72 pubmed publisher
western blot; rat	Tse K, Chow K, Leung W, Wong Y, Wise H. Lipopolysaccharide differentially modulates expression of cytokines and cyclooxygenases in dorsal root ganglion cells via Toll-like receptor-4 dependent pathways. Neuroscience. 2014;267:241-51 pubmed publisher
western blot; human; 1:1000; loading ...; fig 3a	Piao H, Yuan Y, Wang M, Sun Y, Liang H, Ma L. ?-catenin acts as a tumour suppressor in E-cadherin-negative basal-like breast cancer by inhibiting NF-?B signalling. Nat Cell Biol. 2014;16:245-54 pubmed publisher
western blot; human	Pavet V, Shlyakhtina Y, He T, Ceschin D, Kohonen P, Perala M, et al. Plasminogen activator urokinase expression reveals TRAIL responsiveness and supports fractional survival of cancer cells. Cell Death Dis. 2014;5:e1043 pubmed publisher
western blot; mouse; 1:1000	Bhaskar K, Maphis N, Xu G, Varvel N, Kokiko Cochran O, Weick J, et al. Microglial derived tumor necrosis factor-? drives Alzheimer's disease-related neuronal cell cycle events. Neurobiol Dis. 2014;62:273-85 pubmed publisher
western blot; mouse; fig 5	Wu J, Huang Z, Ren J, Zhang Z, He P, Li Y, et al. Mlkl knockout mice demonstrate the indispensable role of Mlkl in necroptosis. Cell Res. 2013;23:994-1006 pubmed publisher
western blot; rat	Feng H, Kang C, Dickman J, Koenig R, Awoyinka I, Zhang Y, et al. Training-induced mitochondrial adaptation: role of peroxisome proliferator-activated receptor ? coactivator-1?, nuclear factor-?B and ?-blockade. Exp Physiol. 2013;98:784-95 pubmed publisher
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product information

SKU :

9246S

Product-Name :

Phospho-IκBα (Ser32/36) (5A5) Mouse mAb

Size :

100 ul

Price-(USD) :

274 USD

Species-x-Reactivity :

H, M, R, Mk, (B, Dg, Pg, GP)

Applications :

Western blot

Product-Category :

Immunology and Inflammation

Shipping-Temp :

AMBIENT

Storage-Temp :

-20°C

Product-Type :

Monoclonal Antibody

MW :

Host :

Mouse

Target :

IkB-a (Ser32/Ser36) phosphate

Primary-Protein :

IkB-alpha

Alt-Names :

I-kappa-B-alpha,IKBA,IkB-alpha,IkappaBalpha,MAD-3,MAD3,Major histocompatibility complex enhancer-binding protein MAD3,NF-kappa-B inhibitor alpha,NFKBI,NFKBIA,nuclear factor of kappa light chain gene enhancer in B-cells,nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha

more info or order :

Cell Signaling Technology product webpage