| Published Application/Species/Sample/Dilution | Reference |
|---|
- western blot; fruit fly ; 1:1000; loading ...; fig 1j
| Kierdorf K, Hersperger F, Sharrock J, Vincent C, Ustaoğlu P, Dou J, et al. Muscle function and homeostasis require cytokine inhibition of AKT activity in Drosophila. elife. 2020;9: pubmed publisher |
- western blot; mouse; loading ...; fig 3a
| Gao H, Freeling J, Wu P, Liang A, Wang X, Li Y. UCHL1 regulates muscle fibers and mTORC1 activity in skeletal muscle. Life Sci. 2019;233:116699 pubmed publisher |
- western blot; mouse; loading ...; fig 3b
| Uddin G, Zhang L, Shah S, Fukushima A, Wagg C, Gopal K, et al. Impaired branched chain amino acid oxidation contributes to cardiac insulin resistance in heart failure. Cardiovasc Diabetol. 2019;18:86 pubmed publisher |
- western blot; mouse; loading ...; fig 10f
| Rojek K, Krzemien J, Dolezyczek H, Boguszewski P, Kaczmarek L, Konopka W, et al. Amot and Yap1 regulate neuronal dendritic tree complexity and locomotor coordination in mice. PLoS Biol. 2019;17:e3000253 pubmed publisher |
- western blot; mouse; 1:1000; loading ...; fig 2c
| Wang Z, Feng J, Yu J, Chen G. FKBP12 mediates necroptosis by initiating RIPK1-RIPK3-MLKL signal transduction in response to TNF receptor 1 ligation. J Cell Sci. 2019;132: pubmed publisher |
- western blot; Dictyostelium discoideum; loading ...; fig 3d
| Nichols J, Paschke P, Peak Chew S, Williams T, Tweedy L, Skehel M, et al. The Atypical MAP Kinase ErkB Transmits Distinct Chemotactic Signals through a Core Signaling Module. Dev Cell. 2019;48:491-505.e9 pubmed publisher |
- western blot; human; loading ...; fig ev3e
| Hartleben G, Muller C, Kramer A, Schimmel H, Zidek L, Dornblut C, et al. Tuberous sclerosis complex is required for tumor maintenance in MYC-driven Burkitt's lymphoma. EMBO J. 2018;37: pubmed publisher |
- western blot; mouse; 1:1000; loading ...; fig 7a
| Zhu L, Xie X, Zhang L, Wang H, Jie Z, Zhou X, et al. TBK-binding protein 1 regulates IL-15-induced autophagy and NKT cell survival. Nat Commun. 2018;9:2812 pubmed publisher |
- western blot; mouse; loading ...; fig 3a
| Li H, Ren Y, Mao K, Hua F, Yang Y, Wei N, et al. FTO is involved in Alzheimer's disease by targeting TSC1-mTOR-Tau signaling. Biochem Biophys Res Commun. 2018;498:234-239 pubmed publisher |
- western blot; human; loading ...; fig 3c
| Agod Z, Pazmandi K, Bencze D, Vereb G, Biro T, Szabo A, et al. Signaling Lymphocyte Activation Molecule Family 5 Enhances Autophagy and Fine-Tunes Cytokine Response in Monocyte-Derived Dendritic Cells via Stabilization of Interferon Regulatory Factor 8. Front Immunol. 2018;9:62 pubmed publisher |
- western blot; human; loading ...; fig 5b
| Nan H, Han L, Ma J, Yang C, Su R, He J. STX3 represses the stability of the tumor suppressor PTEN to activate the PI3K-Akt-mTOR signaling and promotes the growth of breast cancer cells. Biochim Biophys Acta Mol Basis Dis. 2018;1864:1684-1692 pubmed publisher |
- western blot; human; loading ...; fig 2c
| Bostner J, Alayev A, Berman A, Fornander T, Nordenskjold B, Holz M, et al. Raptor localization predicts prognosis and tamoxifen response in estrogen receptor-positive breast cancer. Breast Cancer Res Treat. 2018;168:17-27 pubmed publisher |
- western blot; mouse; loading ...; fig 7a
| Qiao A, Jin X, Pang J, Moskophidis D, Mivechi N. The transcriptional regulator of the chaperone response HSF1 controls hepatic bioenergetics and protein homeostasis. J Cell Biol. 2017;216:723-741 pubmed publisher |
- western blot; human; loading ...; fig 1d
| Ruf S, Heberle A, Langelaar Makkinje M, Gelino S, Wilkinson D, Gerbeth C, et al. PLK1 (polo like kinase 1) inhibits MTOR complex 1 and promotes autophagy. Autophagy. 2017;13:486-505 pubmed publisher |
- western blot; human; loading ...; fig 4a
| Zhang L, Dai F, Cui L, Zhou B, Guo Y. Up-regulation of the active form of small GTPase Rab13 promotes macroautophagy in vascular endothelial cells. Biochim Biophys Acta Mol Cell Res. 2017;1864:613-624 pubmed publisher |
- western blot; mouse; loading ...; fig s4a
| Jiang C, Diao F, Sang Y, Xu N, Zhu R, Wang X, et al. GGPP-Mediated Protein Geranylgeranylation in Oocyte Is Essential for the Establishment of Oocyte-Granulosa Cell Communication and Primary-Secondary Follicle Transition in Mouse Ovary. PLoS Genet. 2017;13:e1006535 pubmed publisher |
- western blot; mouse; loading ...; fig 2b
| Zhang H, Wang W, Ren L, Zhao X, Wang Z, Zhuang D, et al. The mTORC2/Akt/NFκB Pathway-Mediated Activation of TRPC6 Participates in Adriamycin-Induced Podocyte Apoptosis. Cell Physiol Biochem. 2016;40:1079-1093 pubmed |
- western blot; human; loading ...; fig 5c
| Jinesh G, Molina J, Huang L, Laing N, Mills G, Bar Eli M, et al. Mitochondrial oligomers boost glycolysis in cancer stem cells to facilitate blebbishield-mediated transformation after apoptosis. Cell Death Discov. 2016;2:16003 pubmed publisher |
- western blot; mouse; loading ...; fig 1e
| Diez H, Benitez M, Fernandez S, Torres Aleman I, Garrido J, Wandosell F. Class I PI3-kinase or Akt inhibition do not impair axonal polarization, but slow down axonal elongation. Biochim Biophys Acta. 2016;1863:2574-2583 pubmed publisher |
- western blot; human; 1:1000; fig 6
| Dirks M, Wall B, Kramer I, Zorenc A, Goessens J, Gijsen A, et al. A single session of neuromuscular electrical stimulation does not augment postprandial muscle protein accretion. Am J Physiol Endocrinol Metab. 2016;311:E278-85 pubmed publisher |
- western blot; mouse; fig 7
| Wang X, Bey A, Katz B, Badea A, Kim N, David L, et al. Altered mGluR5-Homer scaffolds and corticostriatal connectivity in a Shank3 complete knockout model of autism. Nat Commun. 2016;7:11459 pubmed publisher |
- western blot; mouse; loading ...
| Stephenson E, Ragauskas A, Jaligama S, Redd J, Parvathareddy J, Peloquin M, et al. Exposure to environmentally persistent free radicals during gestation lowers energy expenditure and impairs skeletal muscle mitochondrial function in adult mice. Am J Physiol Endocrinol Metab. 2016;310:E1003-15 pubmed publisher |
- western blot; mouse; fig 4
| Han X, Tai H, Wang X, Wang Z, Zhou J, Wei X, et al. AMPK activation protects cells from oxidative stress-induced senescence via autophagic flux restoration and intracellular NAD(+) elevation. Aging Cell. 2016;15:416-27 pubmed publisher |
- western blot; human; fig 7
| Pivonello C, Negri M, De Martino M, Napolitano M, De Angelis C, Provvisiero D, et al. The dual targeting of insulin and insulin-like growth factor 1 receptor enhances the mTOR inhibitor-mediated antitumor efficacy in hepatocellular carcinoma. Oncotarget. 2016;7:9718-31 pubmed publisher |
- western blot; human; loading ...; fig 5b
| Ma H, Su L, Yue H, Yin X, Zhao J, Zhang S, et al. HMBOX1 interacts with MT2A to regulate autophagy and apoptosis in vascular endothelial cells. Sci Rep. 2015;5:15121 pubmed publisher |
- western blot; pigs ; 1:2000
| Columbus D, Steinhoff Wagner J, Suryawan A, Nguyen H, Hernandez Garcia A, Fiorotto M, et al. Impact of prolonged leucine supplementation on protein synthesis and lean growth in neonatal pigs. Am J Physiol Endocrinol Metab. 2015;309:E601-10 pubmed publisher |
- western blot; human; fig 4
| Zhang L, Dai F, Sheng P, Chen Z, Xu Q, Guo Y. Resveratrol analogue 3,4,4'-trihydroxy-trans-stilbene induces apoptosis and autophagy in human non-small-cell lung cancer cells in vitro. Acta Pharmacol Sin. 2015;36:1256-65 pubmed publisher |
- western blot; human; fig s2
| Felzen V, Hiebel C, Koziollek Drechsler I, Reißig S, Wolfrum U, Kögel D, et al. Estrogen receptor α regulates non-canonical autophagy that provides stress resistance to neuroblastoma and breast cancer cells and involves BAG3 function. Cell Death Dis. 2015;6:e1812 pubmed publisher |
- western blot; human; fig 6
- western blot; fruit fly
| Zhang T, Zhou Q, Ogmundsdottir M, Möller K, Siddaway R, Larue L, et al. Mitf is a master regulator of the v-ATPase, forming a control module for cellular homeostasis with v-ATPase and TORC1. J Cell Sci. 2015;128:2938-50 pubmed publisher |
| Nishida H, Ikegami A, Kaneko C, Kakuma H, Nishi H, Tanaka N, et al. Dexamethasone and BCAA Failed to Modulate Muscle Mass and mTOR Signaling in GH-Deficient Rats. PLoS ONE. 2015;10:e0128805 pubmed publisher |
- western blot; fruit fly ; fig 1
| Takáts S, Varga A, Pircs K, Juhasz G. Loss of Drosophila Vps16A enhances autophagosome formation through reduced Tor activity. Autophagy. 2015;11:1209-15 pubmed publisher |
- immunohistochemistry - paraffin section; human; 1:100
| Ren H, Wang J, Yang F, Zhang X, Wang A, Sun L, et al. Cytoplasmic TRAF4 contributes to the activation of p70s6k signaling pathway in breast cancer. Oncotarget. 2015;6:4080-96 pubmed |
- western blot; human; fig 1
| Brandstaetter H, Kishi Itakura C, Tumbarello D, Manstein D, Buss F. Loss of functional MYO1C/myosin 1c, a motor protein involved in lipid raft trafficking, disrupts autophagosome-lysosome fusion. Autophagy. 2014;10:2310-23 pubmed publisher |
| Fiorini C, Cordani M, Gotte G, Picone D, Donadelli M. Onconase induces autophagy sensitizing pancreatic cancer cells to gemcitabine and activates Akt/mTOR pathway in a ROS-dependent manner. Biochim Biophys Acta. 2015;1853:549-60 pubmed publisher |
| Zhang L, Dai F, Cui L, Jing H, Fan P, Tan X, et al. Novel role for TRPC4 in regulation of macroautophagy by a small molecule in vascular endothelial cells. Biochim Biophys Acta. 2015;1853:377-87 pubmed publisher |
- western blot; mouse; loading ...; fig s6a
| Healy M, Chow J, Byrne F, Breen D, Leitinger N, Li C, et al. Dietary effects on liver tumor burden in mice treated with the hepatocellular carcinogen diethylnitrosamine. J Hepatol. 2015;62:599-606 pubmed publisher |
- western blot; human; loading ...; fig 1a
| Alayev A, Berger S, Kramer M, Schwartz N, Holz M. The combination of rapamycin and resveratrol blocks autophagy and induces apoptosis in breast cancer cells. J Cell Biochem. 2015;116:450-7 pubmed publisher |
- western blot; human; fig 4
| Kocher B, White L, Piwnica Worms D. DAPK3 suppresses acini morphogenesis and is required for mouse development. Mol Cancer Res. 2015;13:358-67 pubmed publisher |
| Subramani R, Lopez Valdez R, Arumugam A, Nandy S, Boopalan T, Lakshmanaswamy R. Targeting insulin-like growth factor 1 receptor inhibits pancreatic cancer growth and metastasis. PLoS ONE. 2014;9:e97016 pubmed publisher |
- immunohistochemistry; human; 1:2000
| Jespersen J, Mikkelsen U, Nedergaard A, Thorlund J, Schjerling P, Suetta C, et al. Alterations in molecular muscle mass regulators after 8 days immobilizing Special Forces mission. Scand J Med Sci Sports. 2015;25:175-83 pubmed publisher |
| Oshiro N, Rapley J, Avruch J. Amino acids activate mammalian target of rapamycin (mTOR) complex 1 without changing Rag GTPase guanyl nucleotide charging. J Biol Chem. 2014;289:2658-74 pubmed publisher |
- western blot; human; 1:500
| McNulty S, Flint M, Nichol S, Spiropoulou C. Host mTORC1 signaling regulates andes virus replication. J Virol. 2013;87:912-22 pubmed publisher |
| Wang Y, Wong C, Yan M, Li L, Liu T, Or P, et al. Differential regulation of the pro-inflammatory biomarker, YKL-40/CHI3L1, by PTEN/Phosphoinositide 3-kinase and JAK2/STAT3 pathways in glioblastoma. Cancer Lett. 2018;429:54-65 pubmed publisher |
| Palam L, Mali R, Ramdas B, Srivatsan S, Visconte V, Tiu R, et al. Loss of epigenetic regulator TET2 and oncogenic KIT regulate myeloid cell transformation via PI3K pathway. JCI Insight. 2018;3: pubmed publisher |
| Ye R, Gordillo R, Shao M, Onodera T, Chen Z, Chen S, et al. Intracellular lipid metabolism impairs β cell compensation during diet-induced obesity. J Clin Invest. 2018;128:1178-1189 pubmed publisher |
| Bu Y, Yoshida A, Chitnis N, Altman B, Tameire F, Oran A, et al. A PERK-miR-211 axis suppresses circadian regulators and protein synthesis to promote cancer cell survival. Nat Cell Biol. 2018;20:104-115 pubmed publisher |
| Yalamanchili N, Kriete A, Alfego D, Danowski K, Kari C, Rodeck U. Distinct Cell Stress Responses Induced by ATP Restriction in Quiescent Human Fibroblasts. Front Genet. 2016;7:171 pubmed |
| Tan K, Ho M, Cho H, Yu J, Hung J, Yu A. Fucosylation of LAMP-1 and LAMP-2 by FUT1 correlates with lysosomal positioning and autophagic flux of breast cancer cells. Cell Death Dis. 2016;7:e2347 pubmed publisher |
| Di Camillo B, Carlon A, Eduati F, Toffolo G. A rule-based model of insulin signalling pathway. BMC Syst Biol. 2016;10:38 pubmed publisher |
| Zhang X, Zhang T, Song D, Zhou J, Han R, Qin Z, et al. Endoplasmic reticulum chaperone GRP78 is involved in autophagy activation induced by ischemic preconditioning in neural cells. Mol Brain. 2015;8:20 pubmed publisher |
| Han K, Hwang J, Bae G, Kim S, Kim Y. Akt regulation of Aven contributes to the sensitivity of cancer cells to chemotherapeutic agents. Mol Med Rep. 2015;11:3866-71 pubmed publisher |
| Patursky Polischuk I, Kasir J, Miloslavski R, Hayouka Z, Hausner Hanochi M, Stolovich Rain M, et al. Reassessment of the role of TSC, mTORC1 and microRNAs in amino acids-meditated translational control of TOP mRNAs. PLoS ONE. 2014;9:e109410 pubmed publisher |
| Peng N, Meng N, Wang S, Zhao F, Zhao J, Su L, et al. An activator of mTOR inhibits oxLDL-induced autophagy and apoptosis in vascular endothelial cells and restricts atherosclerosis in apolipoprotein E?/? mice. Sci Rep. 2014;4:5519 pubmed publisher |
| Hatanaka M, Maier B, Sims E, Templin A, Kulkarni R, Evans Molina C, et al. Palmitate induces mRNA translation and increases ER protein load in islet β-cells via activation of the mammalian target of rapamycin pathway. Diabetes. 2014;63:3404-15 pubmed publisher |
| Di Camillo B, Eduati F, Nair S, Avogaro A, Toffolo G. Leucine modulates dynamic phosphorylation events in insulin signaling pathway and enhances insulin-dependent glycogen synthesis in human skeletal muscle cells. BMC Cell Biol. 2014;15:9 pubmed publisher |
| Gough D, Koetz L, Levy D. The MEK-ERK pathway is necessary for serine phosphorylation of mitochondrial STAT3 and Ras-mediated transformation. PLoS ONE. 2013;8:e83395 pubmed publisher |
| Schupp M, Chen F, Briggs E, Rao S, Pelzmann H, Pessentheiner A, et al. Metabolite and transcriptome analysis during fasting suggest a role for the p53-Ddit4 axis in major metabolic tissues. BMC Genomics. 2013;14:758 pubmed publisher |
| Weissinger D, Tagscherer K, Macher Göppinger S, Haferkamp A, Wagener N, Roth W. The soluble Decoy Receptor 3 is regulated by a PI3K-dependent mechanism and promotes migration and invasion in renal cell carcinoma. Mol Cancer. 2013;12:120 pubmed publisher |
| Machado D, Shishido S, Queiroz K, Oliveira D, Faria A, Catharino R, et al. Irradiated riboflavin diminishes the aggressiveness of melanoma in vitro and in vivo. PLoS ONE. 2013;8:e54269 pubmed publisher |
| Nedergaard A, Jespersen J, Pingel J, Christensen B, Sroczynski N, Langberg H, et al. Effects of 2 weeks lower limb immobilization and two separate rehabilitation regimens on gastrocnemius muscle protein turnover signaling and normalization genes. BMC Res Notes. 2012;5:166 pubmed publisher |
| Li Y, Li B, Wan X, Zhang W, Zhong L, Tang S. NMDA receptor activation stimulates transcription-independent rapid wnt5a protein synthesis via the MAPK signaling pathway. Mol Brain. 2012;5:1 pubmed publisher |
| Park J, Arakawa Takeuchi S, Jinno S, Okayama H. Rho-associated kinase connects a cell cycle-controlling anchorage signal to the mammalian target of rapamycin pathway. J Biol Chem. 2011;286:23132-41 pubmed publisher |
| Cardoso C, Groth Pedersen L, Høyer Hansen M, Kirkegaard T, Corcelle E, Andersen J, et al. Depletion of kinesin 5B affects lysosomal distribution and stability and induces peri-nuclear accumulation of autophagosomes in cancer cells. PLoS ONE. 2009;4:e4424 pubmed publisher |
| Guertin D, Stevens D, Saitoh M, Kinkel S, Crosby K, Sheen J, et al. mTOR complex 2 is required for the development of prostate cancer induced by Pten loss in mice. Cancer Cell. 2009;15:148-59 pubmed publisher |
| Dobson T, Kube E, Timmerman S, Krushel L. Identifying intrinsic and extrinsic determinants that regulate internal initiation of translation mediated by the FMR1 5' leader. BMC Mol Biol. 2008;9:89 pubmed publisher |
| Cao R, Lee B, Cho H, Saklayen S, Obrietan K. Photic regulation of the mTOR signaling pathway in the suprachiasmatic circadian clock. Mol Cell Neurosci. 2008;38:312-24 pubmed publisher |
| Jiménez DÃaz L, Géranton S, Passmore G, Leith J, Fisher A, Berliocchi L, et al. Local translation in primary afferent fibers regulates nociception. PLoS ONE. 2008;3:e1961 pubmed publisher |
| Jimeno A, Kulesza P, Wheelhouse J, Chan A, Zhang X, Kincaid E, et al. Dual EGFR and mTOR targeting in squamous cell carcinoma models, and development of early markers of efficacy. Br J Cancer. 2007;96:952-9 pubmed |
| Sarkar S, Floto R, Berger Z, Imarisio S, Cordenier A, Pasco M, et al. Lithium induces autophagy by inhibiting inositol monophosphatase. J Cell Biol. 2005;170:1101-11 pubmed |
