product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb
catalog :
9154S
quantity :
100 ul
price :
274 USD
clonality :
monoclonal
host :
rabbit
antigen modification :
phosphorylated
reactivity :
human, mouse, rat
application :
western blot, immunoprecipitation
citations: 36
Published Application/Species/DilutionReference
  • western blot; human; fig 2
Kuramoto K, Wang N, Fan Y, Zhang W, Schoenen F, Frankowski K, et al. Autophagy activation by novel inducers prevents BECN2-mediated drug tolerance to cannabinoids. Autophagy. 2016;: pubmed
  • western blot; human; 1:1000; fig 3
Hatanaka M, Higashi Y, Kawai K, Su J, Zeng W, Chen X, et al. CD147-targeted siRNA in A375 malignant melanoma cells induces the phosphorylation of EGFR and downregulates cdc25C and MEK phosphorylation. Oncol Lett. 2016;11:2424-2428 pubmed
  • western blot; human; 1:200; fig st1
Treindl F, Ruprecht B, Beiter Y, Schultz S, Döttinger A, Staebler A, et al. A bead-based western for high-throughput cellular signal transduction analyses. Nat Commun. 2016;7:12852 pubmed publisher
  • western blot; mouse; fig 1
Wandinger S, Lahortiga I, Jacobs K, Klammer M, Jordan N, Elschenbroich S, et al. Quantitative Phosphoproteomics Analysis of ERBB3/ERBB4 Signaling. PLoS ONE. 2016;11:e0146100 pubmed publisher
  • western blot; human; fig 3
Subramaniam S, Ozdener M, Abdoul-Azize S, Saito K, Malik B, Maquart G, et al. ERK1/2 activation in human taste bud cells regulates fatty acid signaling and gustatory perception of fat in mice and humans. FASEB J. 2016;30:3489-3500 pubmed
  • western blot; human; 1:5000; fig 2
Chatelle C, Hövermann D, Muller A, Wagner H, Weber W, Radziwill G. Optogenetically controlled RAF to characterize BRAF and CRAF protein kinase inhibitors. Sci Rep. 2016;6:23713 pubmed publisher
  • western blot; human; 1:1000; fig 3
Hernández-Bule M, Martinez-Botas J, Trillo M, Paíno C, Ubeda A. Antiadipogenic effects of subthermal electric stimulation at 448 kHz on differentiating human mesenchymal stem cells. Mol Med Rep. 2016;13:3895-903 pubmed publisher
  • western blot; human; 1:1000; fig 1
Woodfield S, Zhang L, Scorsone K, Liu Y, Zage P. Binimetinib inhibits MEK and is effective against neuroblastoma tumor cells with low NF1 expression. BMC Cancer. 2016;16:172 pubmed publisher
  • western blot; human; fig 1
  • western blot; mouse; fig 5
Chung S, Moon H, Ju H, Kim D, Cho K, Ribback S, et al. Comparison of liver oncogenic potential among human RAS isoforms. Oncotarget. 2016;7:7354-66 pubmed publisher
  • western blot; mouse; fig 4
Boswell B, Musil L. Synergistic interaction between the fibroblast growth factor and bone morphogenetic protein signaling pathways in lens cells. Mol Biol Cell. 2015;26:2561-72 pubmed publisher
  • western blot; human
Bunaciu R, Jensen H, Macdonald R, Latocha D, Varner J, Yen A. 6-Formylindolo(3,2-b)Carbazole (FICZ) Modulates the Signalsome Responsible for RA-Induced Differentiation of HL-60 Myeloblastic Leukemia Cells. PLoS ONE. 2015;10:e0135668 pubmed publisher
  • western blot; mouse
Bao M, Cai Z, Zhang X, Li L, Liu X, Wan N, et al. Dickkopf-3 protects against cardiac dysfunction and ventricular remodelling following myocardial infarction. Basic Res Cardiol. 2015;110:25 pubmed publisher
  • western blot; human; fig S3
Tate C, Mc Entire J, Pallini R, Vakana E, Wyss L, Blosser W, et al. A BMP7 Variant Inhibits Tumor Angiogenesis In Vitro and In Vivo through Direct Modulation of Endothelial Cell Biology. PLoS ONE. 2015;10:e0125697 pubmed publisher
  • western blot; human; 1:1000; fig  6
Tanaka T, Iino M. Sec8 regulates cytokeratin8 phosphorylation and cell migration by controlling the ERK and p38 MAPK signalling pathways. Cell Signal. 2015;27:1110-9 pubmed publisher
  • western blot; human
Tang X, Chen X, Xu Y, Qiao Y, Zhang X, Wang Y, et al. CD166 positively regulates MCAM via inhibition to ubiquitin E3 ligases Smurf1 and βTrCP through PI3K/AKT and c-Raf/MEK/ERK signaling in Bel-7402 hepatocellular carcinoma cells. Cell Signal. 2015;27:1694-702 pubmed publisher
  • western blot; human; fig s6
Pirman N, Barber K, Aerni H, Ma N, Haimovich A, Rogulina S, et al. A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation. Nat Commun. 2015;6:8130 pubmed publisher
  • western blot; human; fig s3
Vogel C, Smit M, Maddalo G, Possik P, Sparidans R, van der Burg S, et al. Cooperative induction of apoptosis in NRAS mutant melanoma by inhibition of MEK and ROCK. Pigment Cell Melanoma Res. 2015;28:307-17 pubmed publisher
  • western blot; human
Eucker T, Samuelson D, Hunzicker-Dunn M, Konkel M. The focal complex of epithelial cells provides a signalling platform for interleukin-8 induction in response to bacterial pathogens. Cell Microbiol. 2014;16:1441-55 pubmed publisher
  • western blot; rat
Yi X, Li X, Zhou Y, Ren S, Wan W, Feng G, et al. Hepatocyte growth factor regulates the TGF-β1-induced proliferation, differentiation and secretory function of cardiac fibroblasts. Int J Mol Med. 2014;34:381-90 pubmed publisher
  • western blot; human
Chapnick D, Liu X. Leader cell positioning drives wound-directed collective migration in TGFβ-stimulated epithelial sheets. Mol Biol Cell. 2014;25:1586-93 pubmed publisher
  • western blot; human
Sun Y, Guo W, Ren T, Liang W, Zhou W, Lu Q, et al. Gli1 inhibition suppressed cell growth and cell cycle progression and induced apoptosis as well as autophagy depending on ERK1/2 activity in human chondrosarcoma cells. Cell Death Dis. 2014;5:e979 pubmed publisher
  • western blot; human
Choi J, Landrette S, Wang T, Evans P, Bacchiocchi A, Bjornson R, et al. Identification of PLX4032-resistance mechanisms and implications for novel RAF inhibitors. Pigment Cell Melanoma Res. 2014;27:253-62 pubmed publisher
  • western blot; mouse; 1:1000
Zhang Y, Zhang X, Gao L, Liu Y, Jiang D, Chen K, et al. Growth/differentiation factor 1 alleviates pressure overload-induced cardiac hypertrophy and dysfunction. Biochim Biophys Acta. 2014;1842:232-44 pubmed publisher
  • western blot; mouse
Dai J, Shen D, Bian Z, Zhou H, Gan H, Zong J, et al. IKKi deficiency promotes pressure overload-induced cardiac hypertrophy and fibrosis. PLoS ONE. 2013;8:e53412 pubmed publisher
  • western blot; human
Paugh B, Zhu X, Qu C, Endersby R, Diaz A, Zhang J, et al. Novel oncogenic PDGFRA mutations in pediatric high-grade gliomas. Cancer Res. 2013;73:6219-29 pubmed publisher
  • western blot; human
Li Y, Takahashi M, Stork P. Ras-mutant cancer cells display B-Raf binding to Ras that activates extracellular signal-regulated kinase and is inhibited by protein kinase A phosphorylation. J Biol Chem. 2013;288:27646-57 pubmed publisher
Fallahi-Sichani M, Moerke N, Niepel M, Zhang T, Gray N, Sorger P. Systematic analysis of BRAF(V600E) melanomas reveals a role for JNK/c-Jun pathway in adaptive resistance to drug-induced apoptosis. Mol Syst Biol. 2015;11:797 pubmed publisher
El-Chaar N, Piccolo S, Boucher K, Cohen A, Chang J, Moos P, et al. Genomic classification of the RAS network identifies a personalized treatment strategy for lung cancer. Mol Oncol. 2014;8:1339-54 pubmed publisher
Cao Y, Xia D, Qi S, Du J, Ma P, Wang S, et al. Epiregulin can promote proliferation of stem cells from the dental apical papilla via MEK/Erk and JNK signalling pathways. Cell Prolif. 2013;46:447-56 pubmed publisher
Feige E, Yacov N, Salem Y, Levi I, Mendel I, Propheta-Meiran O, et al. Inhibition of monocyte chemotaxis by VB-201, a small molecule lecinoxoid, hinders atherosclerosis development in ApoE⁻/⁻ mice. Atherosclerosis. 2013;229:430-9 pubmed publisher
Weisberg E, Ray A, Nelson E, Adamia S, Barrett R, Sattler M, et al. Reversible resistance induced by FLT3 inhibition: a novel resistance mechanism in mutant FLT3-expressing cells. PLoS ONE. 2011;6:e25351 pubmed publisher
Caunt C, McArdle C. Stimulus-induced uncoupling of extracellular signal-regulated kinase phosphorylation from nuclear localization is dependent on docking domain interactions. J Cell Sci. 2010;123:4310-20 pubmed publisher
Satoh T, Smith A, Sarde A, Lu H, Mian S, Mian S, et al. B-RAF mutant alleles associated with Langerhans cell histiocytosis, a granulomatous pediatric disease. PLoS ONE. 2012;7:e33891 pubmed publisher
Cayer M, Samson M, Bertrand C, Dumont N, Drouin M, Jung D. Suppression of protein phosphatase 2A activity enhances Ad5/F35 adenovirus transduction efficiency in normal human B lymphocytes and in Raji cells. J Immunol Methods. 2012;376:113-24 pubmed publisher
Mizuno A, Yasumuro H, Yoshikawa T, Inami W, Chiba C. MEK-ERK signaling in adult newt retinal pigment epithelium cells is strengthened immediately after surgical induction of retinal regeneration. Neurosci Lett. 2012;523:39-44 pubmed publisher
Caunt C, Perett R, Fowkes R, McArdle C. Mechanisms of GnRH-induced extracellular signal-regulated kinase nuclear localization. PLoS ONE. 2012;7:e40077 pubmed publisher
product information
SKU :
9154S
Product-Name :
Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb
Size :
100 ul
Price-(USD) :
274 USD
Species-x-Reactivity :
H, M, R, Mk, Dm, (C)
Applications :
Immunoprecipitation
Product-Category :
MAP Kinase Signaling
Shipping-Temp :
AMBIENT
Storage-Temp :
-20°C
Product-Type :
Monoclonal Antibody
MW :
45
Host :
Rabbit
Target :
MEK1 (Ser218/Ser222) phosphate/MEK2 (Ser222/Ser226) phosphate
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.