Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb | Cell Signaling Technology 9154 product information

product summary

company name :

Cell Signaling Technology

product type :

antibody

product name :

Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb

catalog :

9154

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

clone name :

41G9

reactivity :

hamsters, human, mouse, rat

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section, reverse phase protein lysate microarray, other

more info or order :

Cell Signaling Technology product webpage

citations: 117

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 5c	Kareddula A, Medina D, Petrosky W, Dolfi S, Tereshchenko I, Walton K, et al. The role of chromodomain helicase DNA binding protein 1 (CHD1) in promoting an invasive prostate cancer phenotype. Ther Adv Urol. 2021;13:17562872211022462 pubmed publisher
western blot; human; 1:500-1:1000; loading ...; fig s1a western blot; mouse; 1:500-1:1000; loading ...; fig 1a	Vichas A, Riley A, Nkinsi N, Kamlapurkar S, Parrish P, Lo A, et al. Integrative oncogene-dependency mapping identifies RIT1 vulnerabilities and synergies in lung cancer. Nat Commun. 2021;12:4789 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 7a, 7e	Li D, Chen J, Guo J, Li L, Cai G, Chen S, et al. A phosphorylation of RIPK3 kinase initiates an intracellular apoptotic pathway that promotes prostaglandin2α-induced corpus luteum regression. elife. 2021;10: pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 2e	Catalano A, Adlesic M, Kaltenbacher T, Klar R, Albers J, Seidel P, et al. Sensitivity and Resistance of Oncogenic RAS-Driven Tumors to Dual MEK and ERK Inhibition. Cancers (Basel). 2021;13: pubmed publisher
western blot; mouse; loading ...; fig 5d	Zhou S, Zhang W, Cai G, Ding Y, Wei C, Li S, et al. Myofiber necroptosis promotes muscle stem cell proliferation via releasing Tenascin-C during regeneration. Cell Res. 2020;30:1063-1077 pubmed publisher
western blot; human; loading ...; fig 7e	Lu C, Wei Y, Wang X, Zhang Z, Yin J, Li W, et al. DNA-methylation-mediated activating of lncRNA SNHG12 promotes temozolomide resistance in glioblastoma. Mol Cancer. 2020;19:28 pubmed publisher
western blot; mouse; loading ...	Goswami D, Chen D, Yang Y, Gudla P, Columbus J, Worthy K, et al. Membrane interactions of the globular domain and the hypervariable region of KRAS4b define its unique diffusion behavior. elife. 2020;9: pubmed publisher
western blot; human; loading ...; fig 6b	Yang X, Jiang J, Zhang C, Li Y. Baicalein restrains proliferation, migration, and invasion of human malignant melanoma cells by down-regulating colon cancer associated transcript-1. Braz J Med Biol Res. 2019;52:e8934 pubmed publisher
western blot; human; loading ...; fig 4a	Gu C, Wang L, Zurawski S, Oh S. Signaling Cascade through DC-ASGPR Induces Transcriptionally Active CREB for IL-10 Induction and Immune Regulation. J Immunol. 2019;: pubmed publisher
western blot; human; loading ...; fig e4e	Wang J, Liu Y, Liu Y, Zheng S, Wang X, Zhao J, et al. Time-resolved protein activation by proximal decaging in living systems. Nature. 2019;569:509-513 pubmed publisher
western blot; mouse; loading ...; fig 1b	Wang E, Dai Z, Ferrante A, Drake C, Christiano A. A Subset of TREM2+ Dermal Macrophages Secretes Oncostatin M to Maintain Hair Follicle Stem Cell Quiescence and Inhibit Hair Growth. Cell Stem Cell. 2019;: pubmed publisher
western blot; mouse; loading ...; fig e6d	Yao W, Rose J, Wang W, Seth S, Jiang H, Taguchi A, et al. Syndecan 1 is a critical mediator of macropinocytosis in pancreatic cancer. Nature. 2019;: pubmed publisher
western blot; mouse; 1:2000; loading ...; fig 1e	Castel P, Cheng A, Cuevas Navarro A, Everman D, Papageorge A, Simanshu D, et al. RIT1 oncoproteins escape LZTR1-mediated proteolysis. Science. 2019;363:1226-1230 pubmed publisher
western blot; mouse; loading ...; fig 1h western blot; human; loading ...; fig 1a	Stivala S, Codilupi T, Brkic S, Baerenwaldt A, Ghosh N, Hao Shen H, et al. Targeting compensatory MEK/ERK activation increases JAK inhibitor efficacy in myeloproliferative neoplasms. J Clin Invest. 2019;130:1596-1611 pubmed publisher
western blot; human; 1:1000; loading ...; fig 6a	Dai L, Hu W, Yang Z, Chen D, He B, Chen Y, et al. Upregulated expression of HOXB7 in intrahepatic cholangiocarcinoma is associated with tumor cell metastasis and poor prognosis. Lab Invest. 2019;99:736-748 pubmed publisher
western blot; human; 1:2000; loading ...; fig 3a	Duan S, Koziol White C, Jester W, Nycholat C, Macauley M, Panettieri R, et al. CD33 recruitment inhibits IgE-mediated anaphylaxis and desensitizes mast cells to allergen. J Clin Invest. 2019;129:1387-1401 pubmed publisher
western blot; mouse; loading ...; fig 6a	Ye P, Liu J, Xu W, Liu D, Ding X, Le S, et al. Dual-Specificity Phosphatase 26 Protects Against Nonalcoholic Fatty Liver Disease in Mice Through Transforming Growth Factor Beta-Activated Kinase 1 Suppression. Hepatology. 2019;69:1946-1964 pubmed publisher
western blot; human; loading ...; fig 2a	Bigenzahn J, Collu G, Kartnig F, Pieraks M, Vladimer G, Heinz L, et al. LZTR1 is a regulator of RAS ubiquitination and signaling. Science. 2018;362:1171-1177 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 7a	Cao Y, Xu Y, Auchoybur M, Chen W, He S, Qin W, et al. Regulatory role of IKKɑ in myocardial ischemia/reperfusion injury by the determination of M1 versus M2 polarization of macrophages. J Mol Cell Cardiol. 2018;123:1-12 pubmed publisher
other; human; loading ...; fig 4c	Ng P, Li J, Jeong K, Shao S, Chen H, Tsang Y, et al. Systematic Functional Annotation of Somatic Mutations in Cancer. Cancer Cell. 2018;33:450-462.e10 pubmed publisher
western blot; human; 1:1000; loading ...; fig 1e	Vlachogiannis G, Hedayat S, Vatsiou A, Jamin Y, Fernández Mateos J, Khan K, et al. Patient-derived organoids model treatment response of metastatic gastrointestinal cancers. Science. 2018;359:920-926 pubmed publisher
western blot; mouse; fig 5a	Ambrogio C, Köhler J, Zhou Z, Wang H, Paranal R, Li J, et al. KRAS Dimerization Impacts MEK Inhibitor Sensitivity and Oncogenic Activity of Mutant KRAS. Cell. 2018;172:857-868.e15 pubmed publisher
western blot; human; loading ...; fig 6	Macdonald R, Shrimp J, Jiang H, Zhang L, Lin H, Yen A. Probing the requirement for CD38 in retinoic acid-induced HL-60 cell differentiation with a small molecule dimerizer and genetic knockout. Sci Rep. 2017;7:17406 pubmed publisher
western blot; human; loading ...; fig s5o	Schafer S, Viswanathan S, Widjaja A, Lim W, Moreno Moral A, Delaughter D, et al. IL-11 is a crucial determinant of cardiovascular fibrosis. Nature. 2017;552:110-115 pubmed publisher
western blot; human; loading ...; fig 6f	Jiang X, Bao Y, Liu H, Kou X, Zhang Z, Sun F, et al. VPS34 stimulation of p62 phosphorylation for cancer progression. Oncogene. 2017;36:6850-6862 pubmed publisher
western blot; human; loading ...; fig 3c	Yurugi H, Marini F, Weber C, David K, Zhao Q, Binder H, et al. Targeting prohibitins with chemical ligands inhibits KRAS-mediated lung tumours. Oncogene. 2017;36:4778-4789 pubmed publisher
western blot; human; fig 3e	Gaggianesi M, Turdo A, Chinnici A, Lipari E, Apuzzo T, Benfante A, et al. IL4 Primes the Dynamics of Breast Cancer Progression via DUSP4 Inhibition. Cancer Res. 2017;77:3268-3279 pubmed publisher
western blot; human; loading ...; fig 3a	Juhasz A, Markel S, Gaur S, Liu H, Lu J, Jiang G, et al. NADPH oxidase 1 supports proliferation of colon cancer cells by modulating reactive oxygen species-dependent signal transduction. J Biol Chem. 2017;292:7866-7887 pubmed publisher
western blot; human; loading ...; fig 1b	Sahu U, Choudhury A, Parvez S, Biswas S, Kar S. Induction of intestinal stemness and tumorigenicity by aberrant internalization of commensal non-pathogenic E. coli. Cell Death Dis. 2017;8:e2667 pubmed publisher
reverse phase protein lysate microarray; human; loading ...; fig st6	Cherniack A, Shen H, Walter V, Stewart C, Murray B, Bowlby R, et al. Integrated Molecular Characterization of Uterine Carcinosarcoma. Cancer Cell. 2017;31:411-423 pubmed publisher
western blot; human; loading ...; fig EV3e	Lafont E, Kantari Mimoun C, Dráber P, De Miguel D, Hartwig T, Reichert M, et al. The linear ubiquitin chain assembly complex regulates TRAIL-induced gene activation and cell death. EMBO J. 2017;36:1147-1166 pubmed publisher
western blot; human; 1:1000; loading ...; fig 5c	Fu S, Xu H, Gu M, Liu C, Wang Q, Wan X, et al. Adiponectin deficiency contributes to the development and progression of benign prostatic hyperplasia in obesity. Sci Rep. 2017;7:43771 pubmed publisher
western blot; mouse; loading ...; fig 7a	Xiong X, Liu Y, Mei Y, Peng J, Wang Z, Kong B, et al. Novel Protective Role of Myeloid Differentiation 1 in Pathological Cardiac Remodelling. Sci Rep. 2017;7:41857 pubmed publisher
western blot; human; loading ...; fig 5b	Wang T, Yu H, Hughes N, Liu B, Kendirli A, Klein K, et al. Gene Essentiality Profiling Reveals Gene Networks and Synthetic Lethal Interactions with Oncogenic Ras. Cell. 2017;168:890-903.e15 pubmed publisher
reverse phase protein lysate microarray; human; loading ...; fig 3a	. Integrated genomic and molecular characterization of cervical cancer. Nature. 2017;543:378-384 pubmed publisher
western blot; human; 1:1000; loading ...; fig s4d	Jiu Y, Peranen J, Schaible N, Cheng F, Eriksson J, Krishnan R, et al. Vimentin intermediate filaments control actin stress fiber assembly through GEF-H1 and RhoA. J Cell Sci. 2017;130:892-902 pubmed publisher
western blot; mouse; loading ...; fig 2a	Kidger A, Rushworth L, Stellzig J, Davidson J, Bryant C, Bayley C, et al. Dual-specificity phosphatase 5 controls the localized inhibition, propagation, and transforming potential of ERK signaling. Proc Natl Acad Sci U S A. 2017;114:E317-E326 pubmed publisher
western blot; rat; 1:1000; loading ...; fig 4a	Ye Y, Zhao Z, Xu H, Zhang X, Su X, Yang Y, et al. Activation of Sphingosine 1-Phosphate Receptor 1 Enhances Hippocampus Neurogenesis in a Rat Model of Traumatic Brain Injury: An Involvement of MEK/Erk Signaling Pathway. Neural Plast. 2016;2016:8072156 pubmed publisher
western blot; human; loading ...	Hill S, Nesser N, Johnson Camacho K, Jeffress M, Johnson A, Boniface C, et al. Context Specificity in Causal Signaling Networks Revealed by Phosphoprotein Profiling. Cell Syst. 2017;4:73-83.e10 pubmed publisher
western blot; mouse; loading ...; fig 7a	Chao M, Guo J, Cheng W, Zhu X, She Z, Huang Z, et al. Loss of Caspase-Activated DNase Protects Against Atherosclerosis in Apolipoprotein E-Deficient Mice. J Am Heart Assoc. 2016;5: pubmed publisher
western blot; human; fig 1c	Vakana E, Pratt S, Blosser W, Dowless M, Simpson N, Yuan X, et al. LY3009120, a panRAF inhibitor, has significant anti-tumor activity in BRAF and KRAS mutant preclinical models of colorectal cancer. Oncotarget. 2017;8:9251-9266 pubmed publisher
western blot; human; loading ...; fig 5a	Li Y, Buijs Gladdines J, Cant Barrett K, Stubbs A, Vroegindeweij E, Smits W, et al. IL-7 Receptor Mutations and Steroid Resistance in Pediatric T cell Acute Lymphoblastic Leukemia: A Genome Sequencing Study. PLoS Med. 2016;13:e1002200 pubmed publisher
western blot; human; 1:5000; loading ...; fig 4d	Mitra S, Ghosh B, Gayen N, Roy J, Mandal A. Bipartite Role of Heat Shock Protein 90 (Hsp90) Keeps CRAF Kinase Poised for Activation. J Biol Chem. 2016;291:24579-24593 pubmed
western blot; human; 1:200; fig st1	Treindl F, Ruprecht B, Beiter Y, Schultz S, Döttinger A, Staebler A, et al. A bead-based western for high-throughput cellular signal transduction analyses. Nat Commun. 2016;7:12852 pubmed publisher
western blot; human; loading ...; fig 1b	Lagares Tena L, GarcÃa MonclÃºs S, LÃ³pez Alemany R, Almacellas Rabaiget O, Huertas MartÃnez J, SÃ¡inz Jaspeado M, et al. Caveolin-1 promotes Ewing sarcoma metastasis regulating MMP-9 expression through MAPK/ERK pathway. Oncotarget. 2016;7:56889-56903 pubmed publisher
western blot; human; fig 3	Subramaniam S, Ozdener M, Abdoul Azize S, Saito K, Malik B, Maquart G, et al. ERK1/2 activation in human taste bud cells regulates fatty acid signaling and gustatory perception of fat in mice and humans. FASEB J. 2016;30:3489-3500 pubmed
western blot; human; fig 2a	Kemper K, Krijgsman O, Kong X, Cornelissen Steijger P, Shahrabi A, Weeber F, et al. BRAF(V600E) Kinase Domain Duplication Identified in Therapy-Refractory Melanoma Patient-Derived Xenografts. Cell Rep. 2016;16:263-277 pubmed publisher
western blot; human; fig 2	Kuramoto K, Wang N, Fan Y, Zhang W, Schoenen F, Frankowski K, et al. Autophagy activation by novel inducers prevents BECN2-mediated drug tolerance to cannabinoids. Autophagy. 2016;12:1460-71 pubmed publisher
western blot; human; 1:1000; fig s4	Wang J, Farris A, Xu K, Wang P, Zhang X, Duong D, et al. GPRC5A suppresses protein synthesis at the endoplasmic reticulum to prevent radiation-induced lung tumorigenesis. Nat Commun. 2016;7:11795 pubmed publisher
western blot; human; loading ...; fig 4c	Zhou B, Ritt D, Morrison D, Der C, Cox A. Protein Kinase CK2? Maintains Extracellular Signal-regulated Kinase (ERK) Activity in a CK2? Kinase-independent Manner to Promote Resistance to Inhibitors of RAF and MEK but Not ERK in BRAF Mutant Melanoma. J Biol Chem. 2016;291:17804-15 pubmed publisher
western blot; mouse; loading ...; fig 1c	Cant Barrett K, Spijkers Hagelstein J, Buijs Gladdines J, Uitdehaag J, Smits W, van der Zwet J, et al. MEK and PI3K-AKT inhibitors synergistically block activated IL7 receptor signaling in T-cell acute lymphoblastic leukemia. Leukemia. 2016;30:1832-43 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 6a	Xu Z, Mei F, Liu H, Sun C, Zheng Z. C-C Motif Chemokine Receptor 9 Exacerbates Pressure Overload-Induced Cardiac Hypertrophy and Dysfunction. J Am Heart Assoc. 2016;5: pubmed publisher
western blot; human; 1:1000; fig 3	Hatanaka M, Higashi Y, Kawai K, Su J, Zeng W, Chen X, et al. CD147-targeted siRNA in A375 malignant melanoma cells induces the phosphorylation of EGFR and downregulates cdc25C and MEK phosphorylation. Oncol Lett. 2016;11:2424-2428 pubmed
western blot; human; 1:1000; fig 3	Hernández Bule M, Martinez Botas J, Trillo M, Paíno C, Ubeda A. Antiadipogenic effects of subthermal electric stimulation at 448 kHz on differentiating human mesenchymal stem cells. Mol Med Rep. 2016;13:3895-903 pubmed publisher
western blot; human; 1:5000; fig 2	Chatelle C, Hövermann D, Muller A, Wagner H, Weber W, Radziwill G. Optogenetically controlled RAF to characterize BRAF and CRAF protein kinase inhibitors. Sci Rep. 2016;6:23713 pubmed publisher
immunohistochemistry - paraffin section; human; 1:400; fig s1	Koussounadis A, Langdon S, Um I, Kay C, Francis K, Harrison D, et al. Dynamic modulation of phosphoprotein expression in ovarian cancer xenograft models. BMC Cancer. 2016;16:205 pubmed publisher
western blot; human; 1:1000; fig 1	Woodfield S, Zhang L, Scorsone K, Liu Y, Zage P. Binimetinib inhibits MEK and is effective against neuroblastoma tumor cells with low NF1 expression. BMC Cancer. 2016;16:172 pubmed publisher
western blot; rat; 1:1000; fig 5	Yu C, Tang L, Liang C, Chen X, Song S, Ding X, et al. Angiotensin-Converting Enzyme 3 (ACE3) Protects Against Pressure Overload-Induced Cardiac Hypertrophy. J Am Heart Assoc. 2016;5: pubmed publisher
western blot; human; fig 1 western blot; mouse; fig 5	Chung S, Moon H, Ju H, Kim D, Cho K, Ribback S, et al. Comparison of liver oncogenic potential among human RAS isoforms. Oncotarget. 2016;7:7354-66 pubmed publisher
western blot; mouse; fig 1	Wandinger S, Lahortiga I, Jacobs K, Klammer M, Jordan N, Elschenbroich S, et al. Quantitative Phosphoproteomics Analysis of ERBB3/ERBB4 Signaling. PLoS ONE. 2016;11:e0146100 pubmed publisher
western blot; human; loading ...; fig 2b	Berges C, Chatterjee M, Topp M, Einsele H. Targeting polo-like kinase 1 suppresses essential functions of alloreactive T cells. Immunol Res. 2016;64:687-98 pubmed publisher
western blot; mouse; fig 5 western blot; human; fig 5	Park Y, Kim S, Kwon T, Kim J, Song I, Shin H, et al. Peroxiredoxin II promotes hepatic tumorigenesis through cooperation with Ras/Forkhead box M1 signaling pathway. Oncogene. 2016;35:3503-13 pubmed publisher
western blot; mouse; fig 4	Yang C, Lowther K, Lalioti M, Seli E. Embryonic Poly(A)-Binding Protein (EPAB) Is Required for Granulosa Cell EGF Signaling and Cumulus Expansion in Female Mice. Endocrinology. 2016;157:405-16 pubmed publisher
western blot; human; fig s6	Pirman N, Barber K, Aerni H, Ma N, Haimovich A, Rogulina S, et al. A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation. Nat Commun. 2015;6:8130 pubmed publisher
western blot; human; fig 6	Gong Y, Qiu W, Ning X, Yang X, Liu L, Wang Z, et al. CCDC34 is up-regulated in bladder cancer and regulates bladder cancer cell proliferation, apoptosis and migration. Oncotarget. 2015;6:25856-67 pubmed publisher
western blot; human	Bunaciu R, Jensen H, Macdonald R, Latocha D, Varner J, Yen A. 6-Formylindolo(3,2-b)Carbazole (FICZ) Modulates the Signalsome Responsible for RA-Induced Differentiation of HL-60 Myeloblastic Leukemia Cells. PLoS ONE. 2015;10:e0135668 pubmed publisher
western blot; hamsters; loading ...; fig 2f	Å olman M, Ligabue A, BlaÅ¾evitÅ¡ O, Jaiswal A, Zhou Y, Liang H, et al. Specific cancer-associated mutations in the switch III region of Ras increase tumorigenicity by nanocluster augmentation. elife. 2015;4:e08905 pubmed publisher
western blot; human; 1:1000; fig 1	Simpson D, Lemonie N, Morgan D, Gaddameedhi S, Kaufmann W. Oncogenic BRAF(V600E) Induces Clastogenesis and UVB Hypersensitivity. Cancers (Basel). 2015;7:1072-90 pubmed publisher
western blot; human; fig 1	Hutchinson K, Johnson D, Johnson A, Sanchez V, Kuba M, Lu P, et al. ERBB activation modulates sensitivity to MEK1/2 inhibition in a subset of driver-negative melanoma. Oncotarget. 2015;6:22348-60 pubmed
western blot; human	Tang X, Chen X, Xu Y, Qiao Y, Zhang X, Wang Y, et al. CD166 positively regulates MCAM via inhibition to ubiquitin E3 ligases Smurf1 and Î²TrCP through PI3K/AKT and c-Raf/MEK/ERK signaling in Bel-7402 hepatocellular carcinoma cells. Cell Signal. 2015;27:1694-702 pubmed publisher
western blot; mouse; fig 4	Boswell B, Musil L. Synergistic interaction between the fibroblast growth factor and bone morphogenetic protein signaling pathways in lens cells. Mol Biol Cell. 2015;26:2561-72 pubmed publisher
western blot; human; fig S3	Tate C, Mc Entire J, Pallini R, Vakana E, Wyss L, Blosser W, et al. A BMP7 Variant Inhibits Tumor Angiogenesis In Vitro and In Vivo through Direct Modulation of Endothelial Cell Biology. PLoS ONE. 2015;10:e0125697 pubmed publisher
western blot; mouse; 1:400; loading ...; fig 6g	Yang Z, Zheng B, Zhang Y, He M, Zhang X, Ma D, et al. miR-155-dependent regulation of mammalian sterile 20-like kinase 2 (MST2) coordinates inflammation, oxidative stress and proliferation in vascular smooth muscle cells. Biochim Biophys Acta. 2015;1852:1477-89 pubmed publisher
western blot; human; fig s5b	Gupta J, Igea A, Papaioannou M, López Casas P, Llonch E, Hidalgo M, et al. Pharmacological inhibition of p38 MAPK reduces tumor growth in patient-derived xenografts from colon tumors. Oncotarget. 2015;6:8539-51 pubmed
western blot; mouse	Bao M, Cai Z, Zhang X, Li L, Liu X, Wan N, et al. Dickkopf-3 protects against cardiac dysfunction and ventricular remodelling following myocardial infarction. Basic Res Cardiol. 2015;110:25 pubmed publisher
reverse phase protein lysate microarray; human; tbl s2	Fallahi Sichani M, Moerke N, Niepel M, Zhang T, Gray N, Sorger P. Systematic analysis of BRAF(V600E) melanomas reveals a role for JNK/c-Jun pathway in adaptive resistance to drug-induced apoptosis. Mol Syst Biol. 2015;11:797 pubmed publisher
western blot; human; fig s3	Vogel C, Smit M, Maddalo G, Possik P, Sparidans R, van der Burg S, et al. Cooperative induction of apoptosis in NRAS mutant melanoma by inhibition of MEK and ROCK. Pigment Cell Melanoma Res. 2015;28:307-17 pubmed publisher
western blot; human; loading ...; fig 2a	Silva G, Aboussekhra A. p16(INK4A) inhibits the pro-metastatic potentials of osteosarcoma cells through targeting the ERK pathway and TGF-Î²1. Mol Carcinog. 2016;55:525-36 pubmed publisher
western blot; human; 1:1000; fig 6	Tanaka T, Iino M. Sec8 regulates cytokeratin8 phosphorylation and cell migration by controlling the ERK and p38 MAPK signalling pathways. Cell Signal. 2015;27:1110-9 pubmed publisher
western blot; human; fig 6	Papadakis A, Sun C, Knijnenburg T, Xue Y, Grernrum W, HÃ¶lzel M, et al. SMARCE1 suppresses EGFR expression and controls responses to MET and ALK inhibitors in lung cancer. Cell Res. 2015;25:445-58 pubmed publisher
western blot; human; loading ...; fig 2g	Yoda A, Adelmant G, Tamburini J, Chapuy B, Shindoh N, Yoda Y, et al. Mutations in G protein Î² subunits promote transformation and kinase inhibitor resistance. Nat Med. 2015;21:71-5 pubmed publisher
western blot; rat	Yi X, Li X, Zhou Y, Ren S, Wan W, Feng G, et al. Hepatocyte growth factor regulates the TGF-?1-induced proliferation, differentiation and secretory function of cardiac fibroblasts. Int J Mol Med. 2014;34:381-90 pubmed publisher
western blot; human	Eucker T, Samuelson D, Hunzicker Dunn M, Konkel M. The focal complex of epithelial cells provides a signalling platform for interleukin-8 induction in response to bacterial pathogens. Cell Microbiol. 2014;16:1441-55 pubmed publisher
western blot; human	Chapnick D, Liu X. Leader cell positioning drives wound-directed collective migration in TGF?-stimulated epithelial sheets. Mol Biol Cell. 2014;25:1586-93 pubmed publisher
western blot; human	Sun Y, Guo W, Ren T, Liang W, Zhou W, Lu Q, et al. Gli1 inhibition suppressed cell growth and cell cycle progression and induced apoptosis as well as autophagy depending on ERK1/2 activity in human chondrosarcoma cells. Cell Death Dis. 2014;5:e979 pubmed publisher
western blot; human; 1:1000	Wang Z, Ren Z, Hu Z, Hu X, Zhang H, Wu H, et al. Angiotensin-II induces phosphorylation of ERK1/2 and promotes aortic adventitial fibroblasts differentiating into myofibroblasts during aortic dissection formation. J Mol Histol. 2014;45:401-12 pubmed publisher
western blot; human	Choi J, Landrette S, Wang T, Evans P, Bacchiocchi A, Bjornson R, et al. Identification of PLX4032-resistance mechanisms and implications for novel RAF inhibitors. Pigment Cell Melanoma Res. 2014;27:253-62 pubmed publisher
western blot; mouse; 1:1000	Zhang Y, Zhang X, Gao L, Liu Y, Jiang D, Chen K, et al. Growth/differentiation factor 1 alleviates pressure overload-induced cardiac hypertrophy and dysfunction. Biochim Biophys Acta. 2014;1842:232-44 pubmed publisher
western blot; human; fig 2a	Paugh B, Zhu X, Qu C, Endersby R, Diaz A, Zhang J, et al. Novel oncogenic PDGFRA mutations in pediatric high-grade gliomas. Cancer Res. 2013;73:6219-29 pubmed publisher
western blot; human	Li Y, Takahashi M, Stork P. Ras-mutant cancer cells display B-Raf binding to Ras that activates extracellular signal-regulated kinase and is inhibited by protein kinase A phosphorylation. J Biol Chem. 2013;288:27646-57 pubmed publisher
western blot; mouse	Dai J, Shen D, Bian Z, Zhou H, Gan H, Zong J, et al. IKKi deficiency promotes pressure overload-induced cardiac hypertrophy and fibrosis. PLoS ONE. 2013;8:e53412 pubmed publisher
	Ran L, Chen Y, Sher J, Wong E, Murphy D, Zhang J, et al. FOXF1 Defines the Core-Regulatory Circuitry in Gastrointestinal Stromal Tumor. Cancer Discov. 2018;8:234-251 pubmed publisher
	Yao Z, Yaeger R, Rodrik Outmezguine V, Tao A, Torres N, Chang M, et al. Tumours with class 3 BRAF mutants are sensitive to the inhibition of activated RAS. Nature. 2017;548:234-238 pubmed publisher
	Tang K, Zhao Y, Li H, Zhu M, Li W, Liu W, et al. Translocase of Inner Membrane 50 Functions as a Novel Protective Regulator of Pathological Cardiac Hypertrophy. J Am Heart Assoc. 2017;6: pubmed publisher
	Manchado E, Weissmueller S, Morris J, Chen C, Wullenkord R, Lujambio A, et al. A combinatorial strategy for treating KRAS-mutant lung cancer. Nature. 2016;534:647-51 pubmed
	Barreto R, Waning D, Gao H, Liu Y, Zimmers T, Bonetto A. Chemotherapy-related cachexia is associated with mitochondrial depletion and the activation of ERK1/2 and p38 MAPKs. Oncotarget. 2016;7:43442-43460 pubmed publisher
	Kang Y, Roife D, Lee Y, Lv H, Suzuki R, Ling J, et al. Transforming Growth Factor-? Limits Secretion of Lumican by Activated Stellate Cells within Primary Pancreatic Adenocarcinoma Tumors. Clin Cancer Res. 2016;22:4934-4946 pubmed
	Ihn H, Lee D, Lee T, Kim S, Shin H, Bae Y, et al. Inhibitory Effects of KP-A159, a Thiazolopyridine Derivative, on Osteoclast Differentiation, Function, and Inflammatory Bone Loss via Suppression of RANKL-Induced MAP Kinase Signaling Pathway. PLoS ONE. 2015;10:e0142201 pubmed publisher
	El Chaar N, Piccolo S, Boucher K, Cohen A, Chang J, Moos P, et al. Genomic classification of the RAS network identifies a personalized treatment strategy for lung cancer. Mol Oncol. 2014;8:1339-54 pubmed publisher
	Hughes A, Spelke D, Xu Z, Kang C, Schaffer D, Herr A. Single-cell western blotting. Nat Methods. 2014;11:749-55 pubmed publisher
	Gao L, Wang X, Wang X, Zhang L, Qiang C, Chang S, et al. IGF-1R, a target of let-7b, mediates crosstalk between IRS-2/Akt and MAPK pathways to promote proliferation of oral squamous cell carcinoma. Oncotarget. 2014;5:2562-74 pubmed
	Feige E, Yacov N, Salem Y, Levi I, Mendel I, Propheta Meiran O, et al. Inhibition of monocyte chemotaxis by VB-201, a small molecule lecinoxoid, hinders atherosclerosis development in ApoE?/? mice. Atherosclerosis. 2013;229:430-9 pubmed publisher
	Cao Y, Xia D, Qi S, Du J, Ma P, Wang S, et al. Epiregulin can promote proliferation of stem cells from the dental apical papilla via MEK/Erk and JNK signalling pathways. Cell Prolif. 2013;46:447-56 pubmed publisher
	Hamilton W, Kaji K, Kunath T. ERK2 suppresses self-renewal capacity of embryonic stem cells, but is not required for multi-lineage commitment. PLoS ONE. 2013;8:e60907 pubmed publisher
	Carnevale J, Ross L, Puissant A, Banerji V, Stone R, DeAngelo D, et al. SYK regulates mTOR signaling in AML. Leukemia. 2013;27:2118-28 pubmed publisher
	Caunt C, Perett R, Fowkes R, McArdle C. Mechanisms of GnRH-induced extracellular signal-regulated kinase nuclear localization. PLoS ONE. 2012;7:e40077 pubmed publisher
	Straussman R, Morikawa T, Shee K, Barzily Rokni M, Qian Z, Du J, et al. Tumour micro-environment elicits innate resistance to RAF inhibitors through HGF secretion. Nature. 2012;487:500-4 pubmed publisher
	Mizuno A, Yasumuro H, Yoshikawa T, Inami W, Chiba C. MEK-ERK signaling in adult newt retinal pigment epithelium cells is strengthened immediately after surgical induction of retinal regeneration. Neurosci Lett. 2012;523:39-44 pubmed publisher
	Jurek B, Slattery D, Maloumby R, Hillerer K, Koszinowski S, Neumann I, et al. Differential contribution of hypothalamic MAPK activity to anxiety-like behaviour in virgin and lactating rats. PLoS ONE. 2012;7:e37060 pubmed publisher
	Satoh T, Smith A, Sarde A, Lu H, Mian S, Mian S, et al. B-RAF mutant alleles associated with Langerhans cell histiocytosis, a granulomatous pediatric disease. PLoS ONE. 2012;7:e33891 pubmed publisher
	Cayer M, Samson M, Bertrand C, Dumont N, Drouin M, Jung D. Suppression of protein phosphatase 2A activity enhances Ad5/F35 adenovirus transduction efficiency in normal human B lymphocytes and in Raji cells. J Immunol Methods. 2012;376:113-24 pubmed publisher
	Zhou H, Shen D, Bian Z, Zong J, Deng W, Zhang Y, et al. Activating transcription factor 3 deficiency promotes cardiac hypertrophy, dysfunction, and fibrosis induced by pressure overload. PLoS ONE. 2011;6:e26744 pubmed publisher
	Weisberg E, Ray A, Nelson E, Adamia S, Barrett R, Sattler M, et al. Reversible resistance induced by FLT3 inhibition: a novel resistance mechanism in mutant FLT3-expressing cells. PLoS ONE. 2011;6:e25351 pubmed publisher
	Lee C, Dykema K, Hawkins D, Cherba D, Webb C, Furge K, et al. MEK2 is sufficient but not necessary for proliferation and anchorage-independent growth of SK-MEL-28 melanoma cells. PLoS ONE. 2011;6:e17165 pubmed publisher
	Caunt C, McArdle C. Stimulus-induced uncoupling of extracellular signal-regulated kinase phosphorylation from nuclear localization is dependent on docking domain interactions. J Cell Sci. 2010;123:4310-20 pubmed publisher
	Nolan Stevaux O, Truitt M, Pahler J, Olson P, Guinto C, Lee D, et al. Differential contribution to neuroendocrine tumorigenesis of parallel egfr signaling in cancer cells and pericytes. Genes Cancer. 2010;1:125-41 pubmed publisher
	Hoover A, Strand G, Nowicki P, Anderson M, Vermeer P, Klingelhutz A, et al. Impaired PTPN13 phosphatase activity in spontaneous or HPV-induced squamous cell carcinomas potentiates oncogene signaling through the MAP kinase pathway. Oncogene. 2009;28:3960-70 pubmed publisher

product information

SKU :

9154S

Product-Name :

Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb

Size :

100 ul

Price-(USD) :

274 USD

Species-x-Reactivity :

H, M, R, Mk, Dm, (C)

Applications :

Immunoprecipitation

Product-Category :

MAP Kinase Signaling

Shipping-Temp :

AMBIENT

Storage-Temp :

-20°C

Product-Type :

Monoclonal Antibody

MW :

Host :

Rabbit

Target :

MEK1 (Ser218/Ser222) phosphate/MEK2 (Ser222/Ser226) phosphate

more info or order :

Cell Signaling Technology product webpage