product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
Phospho-Akt1 (Ser473) (D7F10) XP® Rabbit mAb (Akt1 Specific)
catalog :
9018
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
antigen modification :
phosphorylated
clone name :
D7F10
reactivity :
human, mouse, rat
application :
western blot, immunocytochemistry, flow cytometry, western blot knockout validation
citations: 25
Published Application/Species/Sample/DilutionReference
  • western blot knockout validation; human; loading ...; fig 1a
Wang J, Zhao W, Guo H, Fang Y, Stockman S, Bai S, et al. AKT isoform-specific expression and activation across cancer lineages. BMC Cancer. 2018;18:742 pubmed publisher
  • western blot; human; 1:1000; fig 1n
Yin H, Wang J, Li H, Yu Y, Wang X, Lu L, et al. Extracellular matrix protein-1 secretory isoform promotes ovarian cancer through increasing alternative mRNA splicing and stemness. Nat Commun. 2021;12:4230 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 7b
Shang P, Stepicheva N, Teel K, McCauley A, Fitting C, Hose S, et al. βA3/A1-crystallin regulates apical polarity and EGFR endocytosis in retinal pigmented epithelial cells. Commun Biol. 2021;4:850 pubmed publisher
  • western blot; mouse; loading ...; fig 3e
Grundmann S, Schutkowski A, Berger C, Baur A, König B, Stangl G. High-phosphorus diets reduce aortic lesions and cardiomyocyte size and modify lipid metabolism in Ldl receptor knockout mice. Sci Rep. 2020;10:20748 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 2b
Kumar A, Xie L, Ta C, Hinton A, Gunasekar S, Minerath R, et al. SWELL1 regulates skeletal muscle cell size, intracellular signaling, adiposity and glucose metabolism. elife. 2020;9: pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig s9a
Wang M, Tang C, Xing R, Liu X, Han X, Liu Y, et al. WDR81 regulates adult hippocampal neurogenesis through endosomal SARA-TGFβ signaling. Mol Psychiatry. 2018;: pubmed publisher
  • western blot; mouse; loading ...; fig s11a
Ubil E, Caskey L, Holtzhausen A, Hunter D, Story C, Earp H. Tumor-secreted Pros1 inhibits macrophage M1 polarization to reduce antitumor immune response. J Clin Invest. 2018;128:2356-2369 pubmed publisher
  • immunocytochemistry; mouse; loading ...; fig 4f
Protack C, Foster T, Hashimoto T, Yamamoto K, Lee M, Kraehling J, et al. Eph-B4 regulates adaptive venous remodeling to improve arteriovenous fistula patency. Sci Rep. 2017;7:15386 pubmed publisher
  • western blot; human; 1:2000; loading ...; fig 6b
Yoo S, Latifkar A, Cerione R, Antonyak M. Cool-associated Tyrosine-phosphorylated Protein 1 Is Required for the Anchorage-independent Growth of Cervical Carcinoma Cells by Binding Paxillin and Promoting AKT Activation. J Biol Chem. 2017;292:3947-3957 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 5a
He X, Liu Z, Xia Y, Xu J, Lv G, Wang L, et al. HOXB7 overexpression promotes cell proliferation and correlates with poor prognosis in gastric cancer patients by inducing expression of both AKT and MARKs. Oncotarget. 2017;8:1247-1261 pubmed publisher
  • western blot; human; fig 6
Weikel K, Cacicedo J, Ruderman N, Ido Y. Knockdown of GSK3β increases basal autophagy and AMPK signalling in nutrient-laden human aortic endothelial cells. Biosci Rep. 2016;36: pubmed publisher
  • western blot; mouse; 1:1000
Major J, Salih M, Tuana B. Interplay between the E2F pathway and β-adrenergic signaling in the pathological hypertrophic response of myocardium. J Mol Cell Cardiol. 2015;84:179-90 pubmed publisher
  • western blot; mouse; 1:500; fig 5
Jeffery E, Church C, Holtrup B, Colman L, Rodeheffer M. Rapid depot-specific activation of adipocyte precursor cells at the onset of obesity. Nat Cell Biol. 2015;17:376-85 pubmed publisher
  • western blot; mouse; fig 2f
Liu K, Zhao E, Ilyas G, Lalazar G, Lin Y, Haseeb M, et al. Impaired macrophage autophagy increases the immune response in obese mice by promoting proinflammatory macrophage polarization. Autophagy. 2015;11:271-84 pubmed publisher
  • western blot; human; loading ...; fig 1a
Alayev A, Berger S, Kramer M, Schwartz N, Holz M. The combination of rapamycin and resveratrol blocks autophagy and induces apoptosis in breast cancer cells. J Cell Biochem. 2015;116:450-7 pubmed publisher
Zhang X, Wang R, Hu D, Sun X, Fujioka H, Lundberg K, et al. Oligodendroglial glycolytic stress triggers inflammasome activation and neuropathology in Alzheimer's disease. Sci Adv. 2020;6: pubmed publisher
Goddard P, Sanchez Garrido J, Slater S, Kalyan M, Ruano Gallego D, Marches O, et al. Enteropathogenic Escherichia coli Stimulates Effector-Driven Rapid Caspase-4 Activation in Human Macrophages. Cell Rep. 2019;27:1008-1017.e6 pubmed publisher
Ryzhakov G, West N, Franchini F, Clare S, Ilott N, Sansom S, et al. Alpha kinase 1 controls intestinal inflammation by suppressing the IL-12/Th1 axis. Nat Commun. 2018;9:3797 pubmed publisher
Sharma M, Braun R. Cyclical expression of GDNF is required for spermatogonial stem cell homeostasis. Development. 2018;145: pubmed publisher
Jiao X, Velasco Velázquez M, Wang M, Li Z, Rui H, Peck A, et al. CCR5 Governs DNA Damage Repair and Breast Cancer Stem Cell Expansion. Cancer Res. 2018;78:1657-1671 pubmed publisher
Lagrange B, Benaoudia S, Wallet P, Magnotti F, Provost A, Michal F, et al. Human caspase-4 detects tetra-acylated LPS and cytosolic Francisella and functions differently from murine caspase-11. Nat Commun. 2018;9:242 pubmed publisher
Camilli G, Eren E, Williams D, Aimanianda V, Meunier E, Quintin J. Impaired phagocytosis directs human monocyte activation in response to fungal derived β-glucan particles. Eur J Immunol. 2018;48:757-770 pubmed publisher
Zhang Y, Xie L, Gunasekar S, Tong D, Mishra A, Gibson W, et al. SWELL1 is a regulator of adipocyte size, insulin signalling and glucose homeostasis. Nat Cell Biol. 2017;19:504-517 pubmed publisher
Pandya C, Hoda N, Crider A, Peter D, Kutiyanawalla A, Kumar S, et al. Transglutaminase 2 overexpression induces depressive-like behavior and impaired TrkB signaling in mice. Mol Psychiatry. 2017;22:745-753 pubmed publisher
Chen B, Tan Z, Gao J, Wu W, Liu L, Jin W, et al. Hyperphosphorylation of ribosomal protein S6 predicts unfavorable clinical survival in non-small cell lung cancer. J Exp Clin Cancer Res. 2015;34:126 pubmed publisher
product information
SKU :
9018P
Product-Name :
Phospho-Akt1 (Ser473) (D7F10) XP® Rabbit mAb (Akt1 Specific)
Size :
40 ul
Price-(USD) :
135 USD
Species-x-Reactivity :
H, M, R
Applications :
Flow cytometry
Product-Category :
PI3K / Akt Signaling
Shipping-Temp :
AMBIENT
Storage-Temp :
-20°C
Product-Type :
Monoclonal Antibody
MW :
60
Host :
Rabbit
Target :
Akt1 (Ser473) phosphate
Primary-Protein :
Akt1
Alt-Names :
AKT,AKT1,AKT1 kinase,MGC99656,PKB,PKB-ALPHA,PRKBA,Protein kinase B,Proto-oncogene c-Akt,RAC,RAC-ALPHA,RAC-PK-alpha,RAC-alpha serine/threonine-protein kinase,rac protein kinase alpha,v-akt murine thymoma viral oncogene homolog 1
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
https://www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.