product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb
catalog :
8828S
quantity :
100 ul
price :
274 USD
clonality :
monoclonal
host :
rabbit
antigen modification :
phosphorylated
reactivity :
human, mouse, rat
application :
western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
citations: 21
Published Application/Species/DilutionReference
  • immunocytochemistry; human; 1:200; fig s2
Chen P, Qin L, Li G, Tellides G, Simons M. Fibroblast growth factor (FGF) signaling regulates transforming growth factor beta (TGFβ)-dependent smooth muscle cell phenotype modulation. Sci Rep. 2016;6:33407 pubmed publisher
  • western blot; human; fig 5
Min K, Liggett J, Silva G, Wu W, Wang R, Shen R, et al. NAG-1/GDF15 accumulates in the nucleus and modulates transcriptional regulation of the Smad pathway. Oncogene. 2016;35:377-88 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:500; fig 3
Liu J, Akanuma N, Liu C, Naji A, Halff G, Washburn W, et al. TGF-β1 promotes acinar to ductal metaplasia of human pancreatic acinar cells. Sci Rep. 2016;6:30904 pubmed publisher
  • western blot; mouse; fig 2
Kral J, Kuttke M, Schrottmaier W, Birnecker B, Warszawska J, Wernig C, et al. Sustained PI3K Activation exacerbates BLM-induced Lung Fibrosis via activation of pro-inflammatory and pro-fibrotic pathways. Sci Rep. 2016;6:23034 pubmed publisher
  • western blot; human; fig 2
  • western blot; mouse; fig 3
Liu L, Liu X, Ren X, Tian Y, Chen Z, Xu X, et al. Smad2 and Smad3 have differential sensitivity in relaying TGFβ signaling and inversely regulate early lineage specification. Sci Rep. 2016;6:21602 pubmed publisher
  • western blot; mouse; fig 4
Ahmad F, Chung Y, Tang Y, Hockman S, Liu S, Khan Y, et al. Phosphodiesterase 3B (PDE3B) regulates NLRP3 inflammasome in adipose tissue. Sci Rep. 2016;6:28056 pubmed publisher
  • western blot; human
Singh K, Lovren F, Pan Y, Quan A, Ramadan A, Matkar P, et al. The essential autophagy gene ATG7 modulates organ fibrosis via regulation of endothelial-to-mesenchymal transition. J Biol Chem. 2015;290:2547-59 pubmed publisher
  • western blot; human; 1:1000; fig s4i
Maxfield K, Taus P, Corcoran K, Wooten J, Macion J, Zhou Y, et al. Comprehensive functional characterization of cancer-testis antigens defines obligate participation in multiple hallmarks of cancer. Nat Commun. 2015;6:8840 pubmed publisher
  • western blot; human; fig 6
Yan M, Yao C, Chow J, Chang C, Hwang P, Chuang S, et al. Fucoidan Elevates MicroRNA-29b to Regulate DNMT3B-MTSS1 Axis and Inhibit EMT in Human Hepatocellular Carcinoma Cells. Mar Drugs. 2015;13:6099-116 pubmed publisher
  • western blot; human; 1:500; fig 4
Maris P, Blomme A, Palacios A, Costanza B, Bellahcene A, Bianchi E, et al. Asporin Is a Fibroblast-Derived TGF-β1 Inhibitor and a Tumor Suppressor Associated with Good Prognosis in Breast Cancer. PLoS Med. 2015;12:e1001871 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:200; fig 4
Schira J, Schulte M, Döbele C, Wallner C, Abraham S, Daigeler A, et al. Human scaphoid non-unions exhibit increased osteoclast activity compared to adjacent cancellous bone. J Cell Mol Med. 2015;19:2842-50 pubmed publisher
  • western blot; human; 1:300; fig 5
Buczek M, Miles A, Green W, Johnson C, Boocock D, Pockley A, et al. Cytoplasmic PML promotes TGF-β-associated epithelial-mesenchymal transition and invasion in prostate cancer. Oncogene. 2015;: pubmed publisher
  • western blot; rat; 1:1000
Mitchell K, Shah J, Tsytsikova L, Campbell A, Affram K, Symes A. LPS antagonism of TGF-β signaling results in prolonged survival and activation of rat primary microglia. J Neurochem. 2014;129:155-68 pubmed publisher
  • western blot; mouse
Yang X, Han Z, Zhang S, Zhu P, Hao C, Fan T, et al. Chronic restraint stress decreases the repair potential from mesenchymal stem cells on liver injury by inhibiting TGF-β1 generation. Cell Death Dis. 2014;5:e1308 pubmed publisher
  • western blot; human; 1:800
Sun G, Shi L, Li M, Jiang N, Fu L, Guo J. Lefty inhibits glioma growth by suppressing Nodal-activated Smad and ERK1/2 pathways. J Neurol Sci. 2014;347:137-42 pubmed publisher
  • western blot; human
So J, Lin J, Wahler J, Liby K, Sporn M, Suh N. A synthetic triterpenoid CDDO-Im inhibits tumorsphere formation by regulating stem cell signaling pathways in triple-negative breast cancer. PLoS ONE. 2014;9:e107616 pubmed publisher
  • western blot; mouse
Ekman M, Bhattachariya A, Dahan D, Uvelius B, Albinsson S, Swärd K. Mir-29 repression in bladder outlet obstruction contributes to matrix remodeling and altered stiffness. PLoS ONE. 2013;8:e82308 pubmed publisher
Gurung S, Werkmeister J, Gargett C. Inhibition of Transforming Growth Factor-β Receptor signaling promotes culture expansion of undifferentiated human Endometrial Mesenchymal Stem/stromal Cells. Sci Rep. 2015;5:15042 pubmed publisher
Li S, Nie E, Yin Y, Benowitz L, Tung S, Vinters H, et al. GDF10 is a signal for axonal sprouting and functional recovery after stroke. Nat Neurosci. 2015;18:1737-45 pubmed publisher
Dong X, Zhang C, Ma S, Wen H. High concentrations of mast cell chymase facilitate the transduction of the transforming growth factor-β1/Smads signaling pathway in skin fibroblasts. Exp Ther Med. 2015;9:955-960 pubmed
Wu C, Jiao H, Lai Y, Zheng W, Chen K, Qu H, et al. Kindlin-2 controls TGF-β signalling and Sox9 expression to regulate chondrogenesis. Nat Commun. 2015;6:7531 pubmed publisher
product information
SKU :
8828S
Product-Name :
Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb
Size :
100 ul
Price-(USD) :
274 USD
Species-x-Reactivity :
H, M, R, Mk
Applications :
Flow cytometry
Product-Category :
Developmental Biology
Shipping-Temp :
AMBIENT
Storage-Temp :
-20°C
Product-Type :
Monoclonal Antibody
MW :
52, 60
Host :
Rabbit
Target :
Smad2 (Ser465/Ser467) phosphate/Smad3 (Ser423/Ser425) phosphate
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.