| Published Application/Species/Sample/Dilution | Reference |
|---|
- western blot knockout validation; human; 1:1000; fig 1
| Liu Z, Hui Y, Shi L, Chen Z, Xu X, Chi L, et al. Efficient CRISPR/Cas9-Mediated Versatile, Predictable, and Donor-Free Gene Knockout in Human Pluripotent Stem Cells. Stem Cell Reports. 2016;7:496-507 pubmed publisher |
- western blot; mouse; loading ...; fig 3g, 6c
- western blot; human; loading ...; fig 2g, 6a
| Gu P, Wang D, Zhang J, Wang X, Chen Z, Gu L, et al. Protective function of interleukin-22 in pulmonary fibrosis. Clin Transl Med. 2021;11:e509 pubmed publisher |
- western blot; mouse; loading ...; fig 7a
| Bansod S, Saifi M, Godugu C. Inhibition of discoidin domain receptors by imatinib prevented pancreatic fibrosis demonstrated in experimental chronic pancreatitis model. Sci Rep. 2021;11:12894 pubmed publisher |
- western blot; human; 1:1000; loading ...; fig 7a
| Kariya Y, Oyama M, Suzuki T, Kariya Y. αvβ3 Integrin induces partial EMT independent of TGF-β signaling. Commun Biol. 2021;4:490 pubmed publisher |
- western blot; mouse; 1:1000; loading ...; fig 5h
| Qin L, Fu X, Ma J, Lin M, Zhang P, Wang Y, et al. Kindlin-2 mediates mechanotransduction in bone by regulating expression of Sclerostin in osteocytes. Commun Biol. 2021;4:402 pubmed publisher |
- western blot; human; 1:1000; loading ...; fig 3a
| Chiavarina B, Costanza B, Ronca R, Blomme A, Rezzola S, Chiodelli P, et al. Metastatic colorectal cancer cells maintain the TGFβ program and use TGFBI to fuel angiogenesis. Theranostics. 2021;11:1626-1640 pubmed publisher |
- western blot; mouse; 1:300; loading ...; fig 11c
| Suzuki K, Matsumoto M, Katoh Y, Liu L, Ochiai K, Aizawa Y, et al. Bach1 promotes muscle regeneration through repressing Smad-mediated inhibition of myoblast differentiation. PLoS ONE. 2020;15:e0236781 pubmed publisher |
- western blot; mouse; loading ...; fig 1e
| Lee J, Hall J, Kroehling L, Wu L, Najar T, Nguyen H, et al. Serum Amyloid A Proteins Induce Pathogenic Th17 Cells and Promote Inflammatory Disease. Cell. 2020;180:79-91.e16 pubmed publisher |
- western blot; mouse; loading ...; fig 4a
| Fan Q, He W, Gayen M, Benoit M, Luo X, Hu X, et al. Activated CX3CL1/Smad2 Signals Prevent Neuronal Loss and Alzheimer's Tau Pathology-Mediated Cognitive Dysfunction. J Neurosci. 2020;40:1133-1144 pubmed publisher |
- western blot; human; 1:1000; loading ...; fig 2i, s2b
| Kuninty P, Bansal R, de Geus S, Mardhian D, Schnittert J, van Baarlen J, et al. ITGA5 inhibition in pancreatic stellate cells attenuates desmoplasia and potentiates efficacy of chemotherapy in pancreatic cancer. Sci Adv. 2019;5:eaax2770 pubmed publisher |
- immunohistochemistry; mouse; 1:400; loading ...; fig s8i
| Padmanaban V, Krol I, Suhail Y, Szczerba B, Aceto N, Bader J, et al. E-cadherin is required for metastasis in multiple models of breast cancer. Nature. 2019;573:439-444 pubmed publisher |
- western blot; human; loading ...; fig 3b
| Hori A, Shimoda M, Naoi Y, Kagara N, Tanei T, Miyake T, et al. Vasculogenic mimicry is associated with trastuzumab resistance of HER2-positive breast cancer. Breast Cancer Res. 2019;21:88 pubmed publisher |
- western blot; mouse; loading ...; fig 1s1f
| Hamaguchi M, Muramatsu R, Fujimura H, Mochizuki H, Kataoka H, Yamashita T. Circulating transforming growth factor-β1 facilitates remyelination in the adult central nervous system. elife. 2019;8: pubmed publisher |
- western blot; human; loading ...; fig 7b
| Wei X, Guo J, Li Q, Jia Q, Jing Q, Li Y, et al. Bach1 regulates self-renewal and impedes mesendodermal differentiation of human embryonic stem cells. Sci Adv. 2019;5:eaau7887 pubmed publisher |
- western blot; rat; 1:1000; loading ...; fig 7a
- western blot; human; 1:1000; loading ...; fig 5d
| Wang M, Xiong L, Jiang L, Lu Y, Liu F, Song L, et al. miR-4739 mediates pleural fibrosis by targeting bone morphogenetic protein 7. EBioMedicine. 2019;41:670-682 pubmed publisher |
- western blot; mouse; loading ...; fig 4c
| Yin J, Wang Y, Chang J, Li B, Zhang J, Liu Y, et al. Apelin inhibited epithelial-mesenchymal transition of podocytes in diabetic mice through downregulating immunoproteasome subunits β5i. Cell Death Dis. 2018;9:1031 pubmed publisher |
- western blot; rat; 1:1000; loading ...; fig 6a
| Zhang R, Wu Y, Xie F, Zhong Y, Wang Y, Xu M, et al. RGMa mediates reactive astrogliosis and glial scar formation through TGF?1/Smad2/3 signaling after stroke. Cell Death Differ. 2018;25:1503-1516 pubmed publisher |
- immunocytochemistry; human; fig 3a
- western blot; human; fig 3b
| Bermeo S, Al Saedi A, Kassem M, Vidal C, Duque G. The Role of the Nuclear Envelope Protein MAN1 in Mesenchymal Stem Cell Differentiation. J Cell Biochem. 2017;118:4425-4435 pubmed publisher |
- western blot; mouse; loading ...; fig 1c
| Dahan J, Levillayer F, Xia T, Nouet Y, Werts C, Fanton d Andon M, et al. LIM-Only Protein FHL2 Is a Negative Regulator of Transforming Growth Factor ?1 Expression. Mol Cell Biol. 2017;37: pubmed publisher |
- immunohistochemistry; rat; 1:100; fig 9a
- western blot; rat; 1:1000; fig 8a
| Shu D, Wojciechowski M, Lovicu F. Bone Morphogenetic Protein-7 Suppresses TGF?2-Induced Epithelial-Mesenchymal Transition in the Lens: Implications for Cataract Prevention. Invest Ophthalmol Vis Sci. 2017;58:781-796 pubmed publisher |
- western blot; mouse; 1:1000; loading ...; fig e6
| Mueller A, van Velthoven C, Fukumoto K, Cheung T, Rando T. Intronic polyadenylation of PDGFR? in resident stem cells attenuates muscle fibrosis. Nature. 2016;540:276-279 pubmed publisher |
- chromatin immunoprecipitation; mouse
- western blot; mouse; 1:1000
| Liu W, Zhou L, Zhou C, Zhang S, Jing J, Xie L, et al. GDF11 decreases bone mass by stimulating osteoclastogenesis and inhibiting osteoblast differentiation. Nat Commun. 2016;7:12794 pubmed publisher |
- western blot; human; 1:500; loading ...; fig 5a
| Xiao X, Senavirathna L, Gou X, Huang C, Liang Y, Liu L. EZH2 enhances the differentiation of fibroblasts into myofibroblasts in idiopathic pulmonary fibrosis. Physiol Rep. 2016;4: pubmed publisher |
- western blot; human; 1:1000; loading ...; fig 3c
| Hoare M, Ito Y, Kang T, Weekes M, Matheson N, Patten D, et al. NOTCH1 mediates a switch between two distinct secretomes during senescence. Nat Cell Biol. 2016;18:979-92 pubmed publisher |
- western blot; mouse; 1:1000; fig s2
| Bianchi E, Boekelheide K, Sigman M, Lamb D, Hall S, Hwang K. Ghrelin Inhibits Post-Operative Adhesions via Blockage of the TGF-β Signaling Pathway. PLoS ONE. 2016;11:e0153968 pubmed publisher |
- western blot; human; fig 4b
| Thongon N, Castiglioni I, Zucal C, Latorre E, D Agostino V, Bauer I, et al. The GSK3β inhibitor BIS I reverts YAP-dependent EMT signature in PDAC cell lines by decreasing SMADs expression level. Oncotarget. 2016;7:26551-66 pubmed publisher |
- western blot; human; fig 1
- western blot; mouse; fig 3
| Liu L, Liu X, Ren X, Tian Y, Chen Z, Xu X, et al. Smad2 and Smad3 have differential sensitivity in relaying TGFβ signaling and inversely regulate early lineage specification. Sci Rep. 2016;6:21602 pubmed publisher |
- immunoprecipitation; human; fig 4
- western blot; human; fig 4
| Wang G, Yu Y, Sun C, Liu T, Liang T, Zhan L, et al. STAT3 selectively interacts with Smad3 to antagonize TGF-β signalling. Oncogene. 2016;35:4388-98 pubmed publisher |
- western blot; mouse; 1:1000; fig 7
| Quigley H, Pitha I, Welsbie D, Nguyen C, Steinhart M, Nguyen T, et al. Losartan Treatment Protects Retinal Ganglion Cells and Alters Scleral Remodeling in Experimental Glaucoma. PLoS ONE. 2015;10:e0141137 pubmed publisher |
- immunocytochemistry; human; 1:2000; tbl 4
| Nath A, Li I, Roberts L, Chan C. Elevated free fatty acid uptake via CD36 promotes epithelial-mesenchymal transition in hepatocellular carcinoma. Sci Rep. 2015;5:14752 pubmed publisher |
- western blot; human; fig s2d
| Pelish H, Liau B, Nitulescu I, Tangpeerachaikul A, Poss Z, Da Silva D, et al. Mediator kinase inhibition further activates super-enhancer-associated genes in AML. Nature. 2015;526:273-276 pubmed publisher |
- western blot; human; 1:1000; fig 4
| Maris P, Blomme A, Palacios A, Costanza B, Bellahcène A, Bianchi E, et al. Asporin Is a Fibroblast-Derived TGF-β1 Inhibitor and a Tumor Suppressor Associated with Good Prognosis in Breast Cancer. PLoS Med. 2015;12:e1001871 pubmed publisher |
- immunohistochemistry; mouse; 1:300; fig 5
| Wu C, Jiao H, Lai Y, Zheng W, Chen K, Qu H, et al. Kindlin-2 controls TGF-β signalling and Sox9 expression to regulate chondrogenesis. Nat Commun. 2015;6:7531 pubmed publisher |
- western blot; human; fig 5
| Min K, Liggett J, Silva G, Wu W, Wang R, Shen R, et al. NAG-1/GDF15 accumulates in the nucleus and modulates transcriptional regulation of the Smad pathway. Oncogene. 2016;35:377-88 pubmed publisher |
- western blot; mouse; fig 13
| Edwards J, Bruno J, Key P, Cheng Y. Absence of chloride intracellular channel 4 (CLIC4) predisposes to acute kidney injury but has minimal impact on recovery. BMC Nephrol. 2014;15:54 pubmed publisher |
| Feng Y, Wu H, Xu Y, Zhang Z, Liu T, Lin X, et al. Zinc finger protein 451 is a novel Smad corepressor in transforming growth factor-? signaling. J Biol Chem. 2014;289:2072-83 pubmed publisher |
- immunoprecipitation; rat
- western blot; rat
| Yuan H, Reddy M, Sun G, Lanting L, Wang M, Kato M, et al. Involvement of p300/CBP and epigenetic histone acetylation in TGF-?1-mediated gene transcription in mesangial cells. Am J Physiol Renal Physiol. 2013;304:F601-13 pubmed publisher |
| Zhou X, Geng L, Wang D, Yi H, Talmon G, Wang J. R-Spondin1/LGR5 Activates TGFβ Signaling and Suppresses Colon Cancer Metastasis. Cancer Res. 2017;77:6589-6602 pubmed publisher |
| Sun G, Guzman E, Balasanyan V, Conner C, Wong K, Zhou H, et al. A molecular signature for anastasis, recovery from the brink of apoptotic cell death. J Cell Biol. 2017;216:3355-3368 pubmed publisher |
| Acharya D, Hainer S, Yoon Y, Wang F, Bach I, Rivera Pérez J, et al. KAT-Independent Gene Regulation by Tip60 Promotes ESC Self-Renewal but Not Pluripotency. Cell Rep. 2017;19:671-679 pubmed publisher |
| Xu M, He J, Li J, Feng W, Zhou H, Wei H, et al. Methyl-CpG-binding domain 3 inhibits epithelial-mesenchymal transition in pancreatic cancer cells via TGF-β/Smad signalling. Br J Cancer. 2017;116:91-99 pubmed publisher |
| Coster A, Thorne C, Wu L, Altschuler S. Examining Crosstalk among Transforming Growth Factor β, Bone Morphogenetic Protein, and Wnt Pathways. J Biol Chem. 2017;292:244-250 pubmed publisher |
| Tian Y, Yu Y, Hou L, Chi J, Mao J, Xia L, et al. Serum deprivation response inhibits breast cancer progression by blocking transforming growth factor-β signaling. Cancer Sci. 2016;107:274-80 pubmed publisher |
| Li B, Su Y, Zheng X, Yang Y, Jiang S, Jiang L. Evidence for an Important Role of Smad-7 in Intervertebral Disc Degeneration. J Interferon Cytokine Res. 2015;35:569-79 pubmed publisher |
| Liu L, Nishio N, Ito S, Tanaka Y, Isobe K. Negative regulation of GADD34 on myofibroblasts during cutaneous wound healing. Biomed Res Int. 2014;2014:137049 pubmed publisher |
| Milani B, Milani F, Park D, Namavari A, Shah J, Amirjamshidi H, et al. Rapamycin inhibits the production of myofibroblasts and reduces corneal scarring after photorefractive keratectomy. Invest Ophthalmol Vis Sci. 2013;54:7424-30 pubmed publisher |
| Maurya V, Jha R, Kumar V, Joshi A, Chadchan S, Mohan J, et al. Transforming growth factor-beta 1 (TGF-B1) liberation from its latent complex during embryo implantation and its regulation by estradiol in mouse. Biol Reprod. 2013;89:84 pubmed publisher |
| Katayama M, Donai K, Sakakibara H, Ohtomo Y, Miyagawa M, Kuroda K, et al. Coffee consumption delays the hepatitis and suppresses the inflammation related gene expression in the Long-Evans Cinnamon rat. Clin Nutr. 2014;33:302-10 pubmed publisher |
