product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
Atg7 (D12B11) Rabbit mAb
catalog :
8558
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
D12B11
reactivity :
human, mouse, rat, bovine, fruit fly
application :
western blot, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section, other, western blot knockout validation
citations: 68
Published Application/Species/Sample/DilutionReference
  • western blot knockout validation; human; loading ...; fig 5a
Princely Abudu Y, Pankiv S, Mathai B, Håkon Lystad A, Bindesbøll C, Brenne H, et al. NIPSNAP1 and NIPSNAP2 Act as "Eat Me" Signals for Mitophagy. Dev Cell. 2019;49:509-525.e12 pubmed publisher
  • western blot knockout validation; mouse; loading ...; fig 1h
Guo H, Chitiprolu M, Roncevic L, Javalet C, Hemming F, Trung M, et al. Atg5 Disassociates the V1V0-ATPase to Promote Exosome Production and Tumor Metastasis Independent of Canonical Macroautophagy. Dev Cell. 2017;43:716-730.e7 pubmed publisher
  • western blot knockout validation; human; 1:1000; loading ...; fig 1e
Goodwin J, Dowdle W, Dejesus R, Wang Z, Bergman P, Kobylarz M, et al. Autophagy-Independent Lysosomal Targeting Regulated by ULK1/2-FIP200 and ATG9. Cell Rep. 2017;20:2341-2356 pubmed publisher
  • western blot knockout validation; mouse; loading ...; fig s1b
Gao Y, Liu Y, Hong L, Yang Z, Cai X, Chen X, et al. Golgi-associated LC3 lipidation requires V-ATPase in noncanonical autophagy. Cell Death Dis. 2016;7:e2330 pubmed publisher
  • western blot; human; loading ...; fig 8d
Yuan T, Annamalai K, Naik S, Lupse B, Geravandi S, Pal A, et al. The Hippo kinase LATS2 impairs pancreatic β-cell survival in diabetes through the mTORC1-autophagy axis. Nat Commun. 2021;12:4928 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 6f
Kasahara Y, Osuka S, Takasaki N, Bayasula -, Koya Y, Nakanishi N, et al. Primate-specific POTE-actin gene could play a role in human folliculogenesis by controlling the proliferation of granulosa cells. Cell Death Discov. 2021;7:186 pubmed publisher
  • western blot; human; 1:1000; fig 8f
Cui Y, Yang S, Wei J, Shea C, Zhong W, Wang F, et al. Autophagy of the m6A mRNA demethylase FTO is impaired by low-level arsenic exposure to promote tumorigenesis. Nat Commun. 2021;12:2183 pubmed publisher
  • western blot; human; loading ...
Brockway S, Wang G, Jackson J, Amici D, Takagishi S, Clutter M, et al. Quantitative and multiplexed chemical-genetic phenotyping in mammalian cells with QMAP-Seq. Nat Commun. 2020;11:5722 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig s2c
Yamamoto K, Venida A, Yano J, Biancur D, Kakiuchi M, Gupta S, et al. Autophagy promotes immune evasion of pancreatic cancer by degrading MHC-I. Nature. 2020;581:100-105 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 7e
Arc Chagnaud C, Py G, Fovet T, Roumanille R, Demangel R, Pagano A, et al. Evaluation of an Antioxidant and Anti-inflammatory Cocktail Against Human Hypoactivity-Induced Skeletal Muscle Deconditioning. Front Physiol. 2020;11:71 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 2c
Moruno Manchon J, Lejault P, Wang Y, McCauley B, Honarpisheh P, Morales Scheihing D, et al. Small-molecule G-quadruplex stabilizers reveal a novel pathway of autophagy regulation in neurons. elife. 2020;9: pubmed publisher
  • western blot; human; loading ...; fig 1e
Dai E, Han L, Liu J, Xie Y, Kroemer G, Klionsky D, et al. Autophagy-dependent ferroptosis drives tumor-associated macrophage polarization via release and uptake of oncogenic KRAS protein. Autophagy. 2020;:1-15 pubmed publisher
  • western blot; rat; loading ...; fig 3a
Huang X, Ni B, Xi Y, Chu X, Zhang R, You H. Protease-activated receptor 2 (PAR-2) antagonist AZ3451 as a novel therapeutic agent for osteoarthritis. Aging (Albany NY). 2019;11:12532-12545 pubmed publisher
  • western blot; human; loading ...; fig 5a
Liu H, Feng X, Yang B, Tong R, Lu Y, Chen D, et al. Dimethyl fumarate suppresses hepatocellular carcinoma progression via activating SOCS3/JAK1/STAT3 signaling pathway. Am J Transl Res. 2019;11:4713-4725 pubmed
  • western blot; mouse; loading ...; fig 2c
Yang M, Chen P, Liu J, Zhu S, Kroemer G, Klionsky D, et al. Clockophagy is a novel selective autophagy process favoring ferroptosis. Sci Adv. 2019;5:eaaw2238 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 7d
Jung S, Choe S, Woo H, Jeong H, An H, Moon H, et al. Autophagic death of neural stem cells mediates chronic stress-induced decline of adult hippocampal neurogenesis and cognitive deficits. Autophagy. 2019;:1-19 pubmed publisher
  • western blot; rat; loading ...; fig 2f
Xie X, Bi H, Lai S, Zhang Y, Li N, Cao H, et al. The immunoproteasome catalytic β5i subunit regulates cardiac hypertrophy by targeting the autophagy protein ATG5 for degradation. Sci Adv. 2019;5:eaau0495 pubmed publisher
  • western blot; human; loading ...; fig e4a
Nassour J, Radford R, Correia A, Fusté J, Schoell B, Jauch A, et al. Autophagic cell death restricts chromosomal instability during replicative crisis. Nature. 2019;565:659-663 pubmed publisher
  • western blot; human; loading ...; fig 3i
Son S, Park S, Lee H, Siddiqi F, Lee J, Menzies F, et al. Leucine Signals to mTORC1 via Its Metabolite Acetyl-Coenzyme A. Cell Metab. 2019;29:192-201.e7 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 6d
Vera Ramirez L, Vodnala S, Nini R, Hunter K, Green J. Autophagy promotes the survival of dormant breast cancer cells and metastatic tumour recurrence. Nat Commun. 2018;9:1944 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 2b
Quaranta V, Rainer C, Nielsen S, Raymant M, Ahmed M, Engle D, et al. Macrophage-Derived Granulin Drives Resistance to Immune Checkpoint Inhibition in Metastatic Pancreatic Cancer. Cancer Res. 2018;78:4253-4269 pubmed publisher
  • other; human; loading ...; fig 4c
Ng P, Li J, Jeong K, Shao S, Chen H, Tsang Y, et al. Systematic Functional Annotation of Somatic Mutations in Cancer. Cancer Cell. 2018;33:450-462.e10 pubmed publisher
  • western blot; human; fig 5b
Viswanath P, Radoul M, Izquierdo Garcia J, Ong W, Luchman H, Cairncross J, et al. 2-Hydroxyglutarate-Mediated Autophagy of the Endoplasmic Reticulum Leads to an Unusual Downregulation of Phospholipid Biosynthesis in Mutant IDH1 Gliomas. Cancer Res. 2018;78:2290-2304 pubmed publisher
  • western blot; human; loading ...; fig s7
Merrill N, Schipper J, Karnes J, Kauffman A, Martin K, Mackeigan J. PI3K-C2? knockdown decreases autophagy and maturation of endocytic vesicles. PLoS ONE. 2017;12:e0184909 pubmed publisher
  • western blot; human; loading ...; fig 10c
Jung J, Nayak A, Schaeffer V, Starzetz T, Kirsch A, Muller S, et al. Multiplex image-based autophagy RNAi screening identifies SMCR8 as ULK1 kinase activity and gene expression regulator. elife. 2017;6: pubmed publisher
  • western blot; human; loading ...; fig 7a
Wang Q, Wu S, Zhu H, Ding Y, Dai X, Ouyang C, et al. Deletion of PRKAA triggers mitochondrial fission by inhibiting the autophagy-dependent degradation of DNM1L. Autophagy. 2017;13:404-422 pubmed publisher
  • western blot; rat; 1:1000; loading ...; fig 4a
Yang M, Wang B, Miao L, Xu X, He X. Autophagy is involved in aldosterone‑induced mesangial cell proliferation. Mol Med Rep. 2016;14:4638-4642 pubmed publisher
  • western blot; human; loading ...; fig 3c
Gómez Puerto M, Verhagen L, Braat A, Lam E, Coffer P, Lorenowicz M. Activation of autophagy by FOXO3 regulates redox homeostasis during osteogenic differentiation. Autophagy. 2016;12:1804-1816 pubmed
  • western blot; mouse; loading ...; fig 6a
Li W, Jin D, Hata M, Takai S, Yamanishi K, Shen W, et al. Dysfunction of mitochondria and deformed gap junctions in the heart of IL-18-deficient mice. Am J Physiol Heart Circ Physiol. 2016;311:H313-25 pubmed publisher
  • western blot; bovine; 1:1000; fig 1
Wohlgemuth S, Ramirez Lee Y, Tao S, Monteiro A, Ahmed B, Dahl G. Short communication: Effect of heat stress on markers of autophagy in the mammary gland during the dry period. J Dairy Sci. 2016;99:4875-4880 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:500; fig 6
Kumar R, Narasimhan M, Shanmugam G, Hong J, Devarajan A, Palaniappan S, et al. Abrogation of Nrf2 impairs antioxidant signaling and promotes atrial hypertrophy in response to high-intensity exercise stress. J Transl Med. 2016;14:86 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 6b
Son S, Cha M, Choi H, Kang S, Choi H, Lee M, et al. Insulin-degrading enzyme secretion from astrocytes is mediated by an autophagy-based unconventional secretory pathway in Alzheimer disease. Autophagy. 2016;12:784-800 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 6b
Wu B, Yu L, Wang Y, Wang H, Li C, Yin Y, et al. Aldehyde dehydrogenase 2 activation in aged heart improves the autophagy by reducing the carbonyl modification on SIRT1. Oncotarget. 2016;7:2175-88 pubmed publisher
  • western blot; mouse; 1:1000; fig 2
Xie C, Ginet V, Sun Y, Koike M, Zhou K, Li T, et al. Neuroprotection by selective neuronal deletion of Atg7 in neonatal brain injury. Autophagy. 2016;12:410-23 pubmed publisher
  • western blot; human; fig 1
Dou Z, Xu C, Donahue G, Shimi T, Pan J, Zhu J, et al. Autophagy mediates degradation of nuclear lamina. Nature. 2015;527:105-9 pubmed publisher
  • western blot; human; fig 4f
Kharaziha P, Chioureas D, Baltatzis G, Fonseca P, Rodriguez P, Gogvadze V, et al. Sorafenib-induced defective autophagy promotes cell death by necroptosis. Oncotarget. 2015;6:37066-82 pubmed publisher
  • western blot; human; fig 7
Barbone D, Follo C, Echeverry N, Gerbaudo V, Klabatsa A, Bueno R, et al. Autophagy Correlates with the Therapeutic Responsiveness of Malignant Pleural Mesothelioma in 3D Models. PLoS ONE. 2015;10:e0134825 pubmed publisher
  • western blot; human; loading ...; fig 4e
Yang S, Lin H, Chang V, Chen C, Liu Y, Wang J, et al. Lovastatin overcomes gefitinib resistance through TNF-α signaling in human cholangiocarcinomas with different LKB1 statuses in vitro and in vivo. Oncotarget. 2015;6:23857-73 pubmed
  • western blot; human; fig 2
Felzen V, Hiebel C, Koziollek Drechsler I, Reißig S, Wolfrum U, Kögel D, et al. Estrogen receptor α regulates non-canonical autophagy that provides stress resistance to neuroblastoma and breast cancer cells and involves BAG3 function. Cell Death Dis. 2015;6:e1812 pubmed publisher
  • western blot; rat
  • immunocytochemistry; human
Zhang L, Wang H, Ding K, Xu J. FTY720 induces autophagy-related apoptosis and necroptosis in human glioblastoma cells. Toxicol Lett. 2015;236:43-59 pubmed publisher
  • immunoprecipitation; human; fig 2a
  • western blot; human; fig 2a
Luo T, Fu J, Xu A, Su B, Ren Y, Li N, et al. PSMD10/gankyrin induces autophagy to promote tumor progression through cytoplasmic interaction with ATG7 and nuclear transactivation of ATG7 expression. Autophagy. 2016;12:1355-71 pubmed publisher
  • western blot; fruit fly
Park S, Lee Y, Pak J, Kim H, Choi H, Kim J, et al. O-GlcNAc modification is essential for the regulation of autophagy in Drosophila melanogaster. Cell Mol Life Sci. 2015;72:3173-83 pubmed publisher
  • western blot; rat; loading ...; fig 5a
McMillan E, Paré M, Baechler B, Graham D, Rush J, Quadrilatero J. Autophagic signaling and proteolytic enzyme activity in cardiac and skeletal muscle of spontaneously hypertensive rats following chronic aerobic exercise. PLoS ONE. 2015;10:e0119382 pubmed publisher
  • western blot; rat; 1:1000
Harris White M, Ferbas K, Johnson M, Eslami P, Poteshkina A, Venkova K, et al. A cell-penetrating ester of the neural metabolite lanthionine ketimine stimulates autophagy through the mTORC1 pathway: Evidence for a mechanism of action with pharmacological implications for neurodegenerative pathologies. Neurobiol Dis. 2015;84:60-8 pubmed publisher
  • western blot; rat; fig 1h
Ma Y, Yang H, Xu L, Huang Y, Dai H, KANG X. Testosterone regulates the autophagic clearance of androgen binding protein in rat Sertoli cells. Sci Rep. 2015;5:8894 pubmed publisher
  • western blot; human; loading ...; fig 4c
Sanjurjo L, Amézaga N, Aran G, Naranjo Gómez M, Arias L, Armengol C, et al. The human CD5L/AIM-CD36 axis: A novel autophagy inducer in macrophages that modulates inflammatory responses. Autophagy. 2015;11:487-502 pubmed publisher
  • western blot; human; 1:1000
Zanotto Filho A, Braganhol E, Klafke K, Figueiró F, Terra S, Paludo F, et al. Autophagy inhibition improves the efficacy of curcumin/temozolomide combination therapy in glioblastomas. Cancer Lett. 2015;358:220-31 pubmed publisher
  • western blot; human; 1:1000; fig 4
Dowdle W, Nyfeler B, Nagel J, Elling R, Liu S, Triantafellow E, et al. Selective VPS34 inhibitor blocks autophagy and uncovers a role for NCOA4 in ferritin degradation and iron homeostasis in vivo. Nat Cell Biol. 2014;16:1069-79 pubmed publisher
  • western blot; human
Zhan Z, Xie X, Cao H, Zhou X, Zhang X, Fan H, et al. Autophagy facilitates TLR4- and TLR3-triggered migration and invasion of lung cancer cells through the promotion of TRAF6 ubiquitination. Autophagy. 2014;10:257-68 pubmed publisher
Wandrer F, Frangez Z, Liebig S, John K, Vondran F, Wedemeyer H, et al. Autophagy alleviates amiodarone-induced hepatotoxicity. Arch Toxicol. 2020;: pubmed publisher
Khabir M, Aliche A, Sureau C, Blanchet M, Labonte P. HDV alters the autophagy process to promote its genome replication. J Virol. 2019;: pubmed publisher
Barroso González J, García Expósito L, Hoang S, Lynskey M, Roncaioli J, Ghosh A, et al. RAD51AP1 Is an Essential Mediator of Alternative Lengthening of Telomeres. Mol Cell. 2019;76:11-26.e7 pubmed publisher
Carugo A, Minelli R, Sapio L, Soeung M, Carbone F, Robinson F, et al. p53 Is a Master Regulator of Proteostasis in SMARCB1-Deficient Malignant Rhabdoid Tumors. Cancer Cell. 2019;35:204-220.e9 pubmed publisher
Mehto S, Jena K, Nath P, Chauhan S, Kolapalli S, Das S, et al. The Crohn's Disease Risk Factor IRGM Limits NLRP3 Inflammasome Activation by Impeding Its Assembly and by Mediating Its Selective Autophagy. Mol Cell. 2019;73:429-445.e7 pubmed publisher
Feng J, Zhang Y, She X, Sun Y, Fan L, Ren X, et al. Hypermethylated gene ANKDD1A is a candidate tumor suppressor that interacts with FIH1 and decreases HIF1α stability to inhibit cell autophagy in the glioblastoma multiforme hypoxia microenvironment. Oncogene. 2019;38:103-119 pubmed publisher
Bhattacharjee S, Bose P, Patel K, Roy S, Gain C, Gowda H, et al. Transcriptional and epigenetic modulation of autophagy promotes EBV oncoprotein EBNA3C induced B-cell survival. Cell Death Dis. 2018;9:605 pubmed publisher
Campbell G, Bruckman R, Herns S, Joshi S, Durden D, Spector S. Induction of autophagy by PI3K/MTOR and PI3K/MTOR/BRD4 inhibitors suppresses HIV-1 replication. J Biol Chem. 2018;293:5808-5820 pubmed publisher
Fu R, Deng Q, Zhang H, Hu X, Li Y, Liu Y, et al. A novel autophagy inhibitor berbamine blocks SNARE-mediated autophagosome-lysosome fusion through upregulation of BNIP3. Cell Death Dis. 2018;9:243 pubmed publisher
Kim S, Yang C, Lee H, Kim J, Kim Y, Kim Y, et al. ESRRA (estrogen-related receptor α) is a key coordinator of transcriptional and post-translational activation of autophagy to promote innate host defense. Autophagy. 2018;14:152-168 pubmed publisher
Sheu S, Chen J, Bee Y, Chen Y, Lin S, Shu C. Differential autophagic effects of vital dyes in retinal pigment epithelial ARPE-19 and photoreceptor 661W cells. PLoS ONE. 2017;12:e0174736 pubmed publisher
Nakajima S, Aikawa C, Nozawa T, Minowa Nozawa A, Toh H, Nakagawa I. Bcl-xL Affects Group A Streptococcus-Induced Autophagy Directly, by Inhibiting Fusion between Autophagosomes and Lysosomes, and Indirectly, by Inhibiting Bacterial Internalization via Interaction with Beclin 1-UVRAG. PLoS ONE. 2017;12:e0170138 pubmed publisher
Bernardo B, Nguyen S, Gao X, Tham Y, Ooi J, Patterson N, et al. Inhibition of miR-154 Protects Against Cardiac Dysfunction and Fibrosis in a Mouse Model of Pressure Overload. Sci Rep. 2016;6:22442 pubmed publisher
Subramani R, GONZALEZ E, Arumugam A, Nandy S, González V, Medel J, et al. Nimbolide inhibits pancreatic cancer growth and metastasis through ROS-mediated apoptosis and inhibition of epithelial-to-mesenchymal transition. Sci Rep. 2016;6:19819 pubmed publisher
Ma Y, Yang H, Dong B, Zou H, Zhou Y, Kong X, et al. Biphasic regulation of autophagy by miR-96 in prostate cancer cells under hypoxia. Oncotarget. 2014;5:9169-82 pubmed
Liu X, Yao J, Tripathi D, Ding Z, Xu Y, Sun M, et al. Autophagy mediates HIF2α degradation and suppresses renal tumorigenesis. Oncogene. 2015;34:2450-60 pubmed publisher
Dang S, Xu H, Xu C, Cai W, Li Q, Cheng Y, et al. Autophagy regulates the therapeutic potential of mesenchymal stem cells in experimental autoimmune encephalomyelitis. Autophagy. 2014;10:1301-15 pubmed publisher
He H, Liu X, Lv L, Liang H, Leng B, Zhao D, et al. Calcineurin suppresses AMPK-dependent cytoprotective autophagy in cardiomyocytes under oxidative stress. Cell Death Dis. 2014;5:e997 pubmed publisher
Di X, Zhang G, Zhang Y, Takeda K, Rivera Rosado L, Zhang B. Accumulation of autophagosomes in breast cancer cells induces TRAIL resistance through downregulation of surface expression of death receptors 4 and 5. Oncotarget. 2013;4:1349-64 pubmed
product information
SKU :
8558S
Product-Name :
Atg7 (D12B11) Rabbit mAb
Size :
100 ul
Price-(USD) :
235 USD
Species-x-Reactivity :
H, M, R, (Mk, B)
Applications :
Immunoprecipitation
Product-Category :
Autophagy
Shipping-Temp :
COLD
Storage-Temp :
-20°C
Product-Type :
Monoclonal Antibody
MW :
78
Host :
Rabbit
Target :
ATG7
Primary-Protein :
ATG7
Alt-Names :
APG7 autophagy 7-like,APG7-like,APG7L,ATG7,ATG7 autophagy related 7 homolog (S. cerevisiae),Autophagy-related protein 7,DKFZp434N0735,GSA7,Ubiquitin-activating enzyme E1-like protein,hAGP7,ubiquitin activating enzyme E1-like protein
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
https://www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.