| Published Application/Species/Sample/Dilution | Reference |
|---|
- immunocytochemistry; human; fig 2c
- western blot; human; 1:100; loading ...; fig 5e
| Bakhoum M, Francis J, Agustinus A, Earlie E, Di Bona M, Abramson D, et al. Loss of polycomb repressive complex 1 activity and chromosomal instability drive uveal melanoma progression. Nat Commun. 2021;12:5402 pubmed publisher |
- western blot; human; 1:1600; loading ...; fig 6g
| Maroni G, Bassal M, Krishnan I, Fhu C, Savova V, Zilionis R, et al. Identification of a targetable KRAS-mutant epithelial population in non-small cell lung cancer. Commun Biol. 2021;4:370 pubmed publisher |
- immunohistochemistry; mouse; 1:200; loading ...
| Hanna R, Flamier A, Barabino A, Bernier G. G-quadruplexes originating from evolutionary conserved L1 elements interfere with neuronal gene expression in Alzheimer's disease. Nat Commun. 2021;12:1828 pubmed publisher |
- western blot; human; 1:5000; loading ...; fig s5g
- immunohistochemistry - paraffin section; mouse; 1:5000; loading ...; fig 2b
- western blot; mouse; 1:5000; loading ...; fig s5c
| Perkail S, Andricovich J, Kai Y, Tzatsos A. BAP1 is a haploinsufficient tumor suppressor linking chronic pancreatitis to pancreatic cancer in mice. Nat Commun. 2020;11:3018 pubmed publisher |
- ChIP-Seq; Xenopus laevis; loading ...; fig 3e
| Kuznetsov J, Agüero T, Owens D, Kurtenbach S, Field M, Durante M, et al. BAP1 regulates epigenetic switch from pluripotency to differentiation in developmental lineages giving rise to BAP1-mutant cancers. Sci Adv. 2019;5:eaax1738 pubmed publisher |
- ChIP-Seq; mouse; ; loading ...; fig s4a
| Turberfield A, Kondo T, Nakayama M, Koseki Y, King H, Koseki H, et al. KDM2 proteins constrain transcription from CpG island gene promoters independently of their histone demethylase activity. Nucleic Acids Res. 2019;47:9005-9023 pubmed publisher |
- chromatin immunoprecipitation; human; loading ...; fig 4d
- western blot; human; 1:1000; loading ...; fig 3e
| Azkanaz M, Rodríguez López A, de Boer B, Huiting W, Angrand P, Vellenga E, et al. Protein quality control in the nucleolus safeguards recovery of epigenetic regulators after heat shock. elife. 2019;8: pubmed publisher |
- immunocytochemistry; human; 1:100; loading ...; fig s2a
| Traynor S, Møllegaard N, Jørgensen M, Brückmann N, Pedersen C, Terp M, et al. Remodeling and destabilization of chromosome 1 pericentromeric heterochromatin by SSX proteins. Nucleic Acids Res. 2019;47:6668-6684 pubmed publisher |
- ChIP-Seq; mouse; loading ...; fig 4p
- immunohistochemistry - frozen section; mouse; loading ...; fig 1a
| Pivetti S, Fernandez Perez D, D Ambrosio A, Barbieri C, Manganaro D, Rossi A, et al. Loss of PRC1 activity in different stem cell compartments activates a common transcriptional program with cell type-dependent outcomes. Sci Adv. 2019;5:eaav1594 pubmed publisher |
- ChIP-Seq; mouse; loading ...; fig 1f
- western blot; mouse; loading ...; fig 1d
| Fursova N, Blackledge N, Nakayama M, Ito S, Koseki Y, Farcas A, et al. Synergy between Variant PRC1 Complexes Defines Polycomb-Mediated Gene Repression. Mol Cell. 2019;74:1020-1036.e8 pubmed publisher |
- chromatin immunoprecipitation; mouse; loading ...; fig 2f
- western blot; mouse; loading ...; fig 2a, 4d, s6f
| He M, Chaurushiya M, Webster J, Kummerfeld S, Reja R, Chaudhuri S, et al. Intrinsic apoptosis shapes the tumor spectrum linked to inactivation of the deubiquitinase BAP1. Science. 2019;364:283-285 pubmed publisher |
- ChIP-Seq; mouse; loading ...; fig 4b
- western blot; mouse; 1:1000; loading ...; fig 1b
| Lavarone E, Barbieri C, Pasini D. Dissecting the role of H3K27 acetylation and methylation in PRC2 mediated control of cellular identity. Nat Commun. 2019;10:1679 pubmed publisher |
- ChIP-Seq; mouse; loading ...; fig 3d
| Kelly M, So J, Rogers A, Gregory G, Li J, Zethoven M, et al. Bcor loss perturbs myeloid differentiation and promotes leukaemogenesis. Nat Commun. 2019;10:1347 pubmed publisher |
- western blot; human; loading ...; fig 5a
| Chen X, Hu L, Yang H, Ma H, Ye K, Zhao C, et al. DHHC protein family targets different subsets of glioma stem cells in specific niches. J Exp Clin Cancer Res. 2019;38:25 pubmed publisher |
- immunocytochemistry; mouse; 1:200; loading ...; fig s6d
| Żylicz J, Bousard A, Zumer K, Dossin F, Mohammad E, da Rocha S, et al. The Implication of Early Chromatin Changes in X Chromosome Inactivation. Cell. 2019;176:182-197.e23 pubmed publisher |
- western blot; human; loading ...; fig 7b
| Zhang Y, Shi J, Liu X, Feng L, Gong Z, Koppula P, et al. BAP1 links metabolic regulation of ferroptosis to tumour suppression. Nat Cell Biol. 2018;20:1181-1192 pubmed publisher |
- ChIP-Seq; human; 1:100; loading ...; fig 6a
| Ning B, Zhao W, Qian C, Liu P, Li Q, Li W, et al. USP26 functions as a negative regulator of cellular reprogramming by stabilising PRC1 complex components. Nat Commun. 2017;8:349 pubmed publisher |
- immunocytochemistry; human; 1:1000; loading ...; fig 4e
- western blot; human; 1:1000; loading ...; fig 4a
| Patel N, Garikapati K, Pandita R, Singh D, Pandita T, Bhadra U, et al. miR-15a/miR-16 down-regulates BMI1, impacting Ub-H2A mediated DNA repair and breast cancer cell sensitivity to doxorubicin. Sci Rep. 2017;7:4263 pubmed publisher |
- western blot; human; loading ...; fig 5b
| Yan Y, Zhao W, Huang Y, Tong H, Xia Y, Jiang Q, et al. Loss of Polycomb Group Protein Pcgf1 Severely Compromises Proper Differentiation of Embryonic Stem Cells. Sci Rep. 2017;7:46276 pubmed publisher |
- chromatin immunoprecipitation; mouse; loading ...; tbl s3
| Lau M, Schwartz M, Kundu S, Savol A, Wang P, Marr S, et al. Mutation of a nucleosome compaction region disrupts Polycomb-mediated axial patterning. Science. 2017;355:1081-1084 pubmed publisher |
- ChIP-Seq; mouse; loading ...; fig 5a
- immunohistochemistry; mouse; 1:200
- western blot; mouse; loading ...; fig 5c
| Jullien J, Vodnala M, Pasque V, Oikawa M, Miyamoto K, Allen G, et al. Gene Resistance to Transcriptional Reprogramming following Nuclear Transfer Is Directly Mediated by Multiple Chromatin-Repressive Pathways. Mol Cell. 2017;65:873-884.e8 pubmed publisher |
- western blot; human; loading ...; fig 2a
| Wheaton K, Sarkari F, Stanly Johns B, Davarinejad H, Egorova O, Kaustov L, et al. UbE2E1/UBCH6 Is a Critical in Vivo E2 for the PRC1-catalyzed Ubiquitination of H2A at Lys-119. J Biol Chem. 2017;292:2893-2902 pubmed publisher |
- immunocytochemistry; mouse; 1:500; fig 2
| Cooper S, Grijzenhout A, Underwood E, Ancelin K, Zhang T, Nesterova T, et al. Jarid2 binds mono-ubiquitylated H2A lysine 119 to mediate crosstalk between Polycomb complexes PRC1 and PRC2. Nat Commun. 2016;7:13661 pubmed publisher |
- chromatin immunoprecipitation; fruit fly ; 1:100; fig s10d
- immunohistochemistry; fruit fly ; 1:500; fig s10b
| Loubiere V, Delest A, Thomas A, Bonev B, Schuettengruber B, Sati S, et al. Coordinate redeployment of PRC1 proteins suppresses tumor formation during Drosophila development. Nat Genet. 2016;48:1436-1442 pubmed publisher |
- chromatin immunoprecipitation; thale cress; fig 4
| Li Z, Li B, Liu J, Guo Z, Liu Y, Li Y, et al. Transcription factors AS1 and AS2 interact with LHP1 to repress KNOX genes in Arabidopsis. J Integr Plant Biol. 2016;58:959-970 pubmed publisher |
- chromatin immunoprecipitation; fruit fly ; loading ...; fig s14c
| Lu C, Jain S, Hoelper D, Bechet D, Molden R, Ran L, et al. Histone H3K36 mutations promote sarcomagenesis through altered histone methylation landscape. Science. 2016;352:844-9 pubmed publisher |
- ChIP-Seq; mouse; fig 5
- western blot; mouse; fig 5
| Si S, Nakajima Takagi Y, Aoyama K, Oshima M, Saraya A, Sugishita H, et al. Loss of Pcgf5 Affects Global H2A Monoubiquitination but Not the Function of Hematopoietic Stem and Progenitor Cells. PLoS ONE. 2016;11:e0154561 pubmed publisher |
- ChIP-Seq; mouse; fig st2
- western blot; mouse; fig s10
| Stelloh C, Reimer M, Pulakanti K, Blinka S, Peterson J, Pinello L, et al. The cohesin-associated protein Wapal is required for proper Polycomb-mediated gene silencing. Epigenetics Chromatin. 2016;9:14 pubmed publisher |
- immunohistochemistry; mouse; 1:250; loading ...; fig 1b
| Eid A, Torres Padilla M. Characterization of non-canonical Polycomb Repressive Complex 1 subunits during early mouse embryogenesis. Epigenetics. 2016;11:389-97 pubmed publisher |
- chromatin immunoprecipitation; mouse; fig 6
| GarcÃa Prat L, MartÃnez Vicente M, Perdiguero E, Ortet L, RodrÃguez Ubreva J, Rebollo E, et al. Autophagy maintains stemness by preventing senescence. Nature. 2016;529:37-42 pubmed publisher |
- chromatin immunoprecipitation; mouse; fig 3
- immunohistochemistry; mouse; 1:400; fig 4
- western blot; mouse; 1:5000; fig 2
| Benitz S, Regel I, Reinhard T, Popp A, Schäffer I, Raulefs S, et al. Polycomb repressor complex 1 promotes gene silencing through H2AK119 mono-ubiquitination in acinar-to-ductal metaplasia and pancreatic cancer cells. Oncotarget. 2016;7:11424-33 pubmed publisher |
- immunohistochemistry - frozen section; mouse; 1:200; fig 2
- chromatin immunoprecipitation; mouse; fig 2
| Mardaryev A, Liu B, Rapisarda V, Poterlowicz K, Malashchuk I, Rudolf J, et al. Cbx4 maintains the epithelial lineage identity and cell proliferation in the developing stratified epithelium. J Cell Biol. 2016;212:77-89 pubmed publisher |
- western blot; human; fig 5
| Daou S, Hammond Martel I, Mashtalir N, Barbour H, Gagnon J, Iannantuono N, et al. The BAP1/ASXL2 Histone H2A Deubiquitinase Complex Regulates Cell Proliferation and Is Disrupted in Cancer. J Biol Chem. 2015;290:28643-63 pubmed publisher |
- chromatin immunoprecipitation; human; fig s7
| Papadakis A, Sun C, Knijnenburg T, Xue Y, Grernrum W, Hölzel M, et al. SMARCE1 suppresses EGFR expression and controls responses to MET and ALK inhibitors in lung cancer. Cell Res. 2015;25:445-58 pubmed publisher |
- western blot; human; fig 1i
- chromatin immunoprecipitation; mouse; fig 1a
| Bhatnagar S, Gazin C, Chamberlain L, Ou J, Zhu X, Tushir J, et al. TRIM37 is a new histone H2A ubiquitin ligase and breast cancer oncoprotein. Nature. 2014;516:116-20 pubmed publisher |
| Hock A, Vigneron A, Vousden K. Ubiquitin-specific peptidase 42 (USP42) functions to deubiquitylate histones and regulate transcriptional activity. J Biol Chem. 2014;289:34862-70 pubmed publisher |
| Ji X, Lu H, Zhou Q, Luo K. LARP7 suppresses P-TEFb activity to inhibit breast cancer progression and metastasis. elife. 2014;3:e02907 pubmed publisher |
- immunocytochemistry; mouse; 1:500; fig 1
- western blot; mouse; 1:1000; fig s1c
| Cooper S, Dienstbier M, Hassan R, Schermelleh L, Sharif J, Blackledge N, et al. Targeting polycomb to pericentric heterochromatin in embryonic stem cells reveals a role for H2AK119u1 in PRC2 recruitment. Cell Rep. 2014;7:1456-1470 pubmed publisher |
- chromatin immunoprecipitation; human
- western blot; human; 1:1000
| Cao Q, Wang X, Zhao M, Yang R, Malik R, Qiao Y, et al. The central role of EED in the orchestration of polycomb group complexes. Nat Commun. 2014;5:3127 pubmed publisher |
- immunoprecipitation; human
| Yuan G, Ma B, Yuan W, Zhang Z, Chen P, Ding X, et al. Histone H2A ubiquitination inhibits the enzymatic activity of H3 lysine 36 methyltransferases. J Biol Chem. 2013;288:30832-42 pubmed publisher |
| Lee S, Phipson B, Hyland C, Leong H, Allan R, Lun A, et al. Polycomb repressive complex 2 (PRC2) suppresses E?-myc lymphoma. Blood. 2013;122:2654-63 pubmed publisher |
| Xiong Z, Xia P, Zhu X, Geng J, Wang S, Ye B, et al. Glutamylation of deubiquitinase BAP1 controls self-renewal of hematopoietic stem cells and hematopoiesis. J Exp Med. 2020;217: pubmed publisher |
| Tamburri S, Lavarone E, Fernandez Perez D, Conway E, Zanotti M, Manganaro D, et al. Histone H2AK119 Mono-Ubiquitination Is Essential for Polycomb-Mediated Transcriptional Repression. Mol Cell. 2019;: pubmed publisher |
| Blackledge N, Fursova N, Kelley J, Huseyin M, Feldmann A, Klose R. PRC1 Catalytic Activity Is Central to Polycomb System Function. Mol Cell. 2019;: pubmed publisher |
| Bianchi M, Crinelli R, Giacomini E, Carloni E, Radici L, Scarpa E, et al. A negative feedback mechanism links UBC gene expression to ubiquitin levels by affecting RNA splicing rather than transcription. Sci Rep. 2019;9:18556 pubmed publisher |
| Healy E, Mucha M, Glancy E, Fitzpatrick D, Conway E, Neikes H, et al. PRC2.1 and PRC2.2 Synergize to Coordinate H3K27 Trimethylation. Mol Cell. 2019;76:437-452.e6 pubmed publisher |
| Lamb K, Bsteh D, Dishman S, Moussa H, Fan H, Stuckey J, et al. Discovery and Characterization of a Cellular Potent Positive Allosteric Modulator of the Polycomb Repressive Complex 1 Chromodomain, CBX7. Cell Chem Biol. 2019;26:1365-1379.e22 pubmed publisher |
| de las Pozas A, Reiner T, De Cesare V, Trost M, Perez Stable C. Inhibiting Multiple Deubiquitinases to Reduce Androgen Receptor Expression in Prostate Cancer Cells. Sci Rep. 2018;8:13146 pubmed publisher |
| Nishioka K, Miyazaki H, Soejima H. Unbiased shRNA screening, using a combination of FACS and high-throughput sequencing, enables identification of novel modifiers of Polycomb silencing. Sci Rep. 2018;8:12128 pubmed publisher |
| Dardalhon Cuménal D, Deraze J, Dupont C, Ribeiro V, Coléno Costes A, Pouch J, et al. Cyclin G and the Polycomb Repressive complexes PRC1 and PR-DUB cooperate for developmental stability. PLoS Genet. 2018;14:e1007498 pubmed publisher |
| Nagase R, Inoue D, Pastore A, Fujino T, Hou H, Yamasaki N, et al. Expression of mutant Asxl1 perturbs hematopoiesis and promotes susceptibility to leukemic transformation. J Exp Med. 2018;215:1729-1747 pubmed publisher |
| Conway E, Jerman E, Healy E, Ito S, Holoch D, Oliviero G, et al. A Family of Vertebrate-Specific Polycombs Encoded by the LCOR/LCORL Genes Balance PRC2 Subtype Activities. Mol Cell. 2018;70:408-421.e8 pubmed publisher |
| Kolluri K, Alifrangis C, Kumar N, Ishii Y, Price S, Michaut M, et al. Loss of functional BAP1 augments sensitivity to TRAIL in cancer cells. elife. 2018;7: pubmed publisher |
| Omori Y, Kubo S, Kon T, Furuhashi M, Narita H, Kominami T, et al. Samd7 is a cell type-specific PRC1 component essential for establishing retinal rod photoreceptor identity. Proc Natl Acad Sci U S A. 2017;114:E8264-E8273 pubmed publisher |
| Dong Y, Isono K, Ohbo K, Endo T, Ohara O, Maekawa M, et al. EPC1/TIP60-Mediated Histone Acetylation Facilitates Spermiogenesis in Mice. Mol Cell Biol. 2017;37: pubmed publisher |
| Endoh M, Endo T, Shinga J, Hayashi K, Farcas A, Ma K, et al. PCGF6-PRC1 suppresses premature differentiation of mouse embryonic stem cells by regulating germ cell-related genes. elife. 2017;6: pubmed publisher |
| Inagaki T, Iwasaki S, Matsumura Y, Kawamura T, Tanaka T, Abe Y, et al. The FBXL10/KDM2B scaffolding protein associates with novel polycomb repressive complex-1 to regulate adipogenesis. J Biol Chem. 2015;290:4163-77 pubmed publisher |
| Basnet H, Su X, Tan Y, Meisenhelder J, Merkurjev D, Ohgi K, et al. Tyrosine phosphorylation of histone H2A by CK2 regulates transcriptional elongation. Nature. 2014;516:267-71 pubmed publisher |
| Ji Z, Mohammed H, Webber A, Ridsdale J, Han N, Carroll J, et al. The forkhead transcription factor FOXK2 acts as a chromatin targeting factor for the BAP1-containing histone deubiquitinase complex. Nucleic Acids Res. 2014;42:6232-42 pubmed publisher |
| Toth Z, Brulois K, Lee H, Izumiya Y, Tepper C, Kung H, et al. Biphasic euchromatin-to-heterochromatin transition on the KSHV genome following de novo infection. PLoS Pathog. 2013;9:e1003813 pubmed publisher |
| Adorno M, Sikandar S, Mitra S, Kuo A, Nicolis Di Robilant B, Haro Acosta V, et al. Usp16 contributes to somatic stem-cell defects in Down's syndrome. Nature. 2013;501:380-4 pubmed publisher |
| Endoh M, Endo T, Endoh T, Isono K, Sharif J, Ohara O, et al. Histone H2A mono-ubiquitination is a crucial step to mediate PRC1-dependent repression of developmental genes to maintain ES cell identity. PLoS Genet. 2012;8:e1002774 pubmed publisher |
