Acetyl-Histone H3 (Lys27) (D5E4) XP® Rabbit mAb | Cell Signaling Technology 8173 product information

product summary

company name :

Cell Signaling Technology

product type :

antibody

product name :

Acetyl-Histone H3 (Lys27) (D5E4) XP® Rabbit mAb

catalog :

8173

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

D5E4

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, immunohistochemistry - paraffin section, ChIP-Seq, other

more info or order :

Cell Signaling Technology product webpage

citations: 23

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:2000; loading ...; fig 2c	Wang Z, Millard C, Lin C, Gurnett J, Wu M, Lee K, et al. Diverse nucleosome site-selectivity among histone deacetylase complexes. elife. 2020;9: pubmed publisher
immunohistochemistry - paraffin section; mouse; loading ...; fig s5h	Cheng C, Biton M, Haber A, Gunduz N, Eng G, Gaynor L, et al. Ketone Body Signaling Mediates Intestinal Stem Cell Homeostasis and Adaptation to Diet. Cell. 2019;178:1115-1131.e15 pubmed publisher
chromatin immunoprecipitation; human; fig 4h	Chen Z, Gao Y, Yao L, Liu Y, Huang L, Yan Z, et al. LncFZD6 initiates Wnt/β-catenin and liver TIC self-renewal through BRG1-mediated FZD6 transcriptional activation. Oncogene. 2018;37:3098-3112 pubmed publisher
ChIP-Seq; mouse; fig 4a	Andricovich J, Perkail S, Kai Y, Casasanta N, Peng W, Tzatsos A. Loss of KDM6A Activates Super-Enhancers to Induce Gender-Specific Squamous-like Pancreatic Cancer and Confers Sensitivity to BET Inhibitors. Cancer Cell. 2018;33:512-526.e8 pubmed publisher
chromatin immunoprecipitation; mouse; loading ...; fig 8a	Shen Y, Kapfhamer D, Minnella A, Kim J, Won S, Chen Y, et al. Bioenergetic state regulates innate inflammatory responses through the transcriptional co-repressor CtBP. Nat Commun. 2017;8:624 pubmed publisher
chromatin immunoprecipitation; human; fig 7b	Walter K, Goodman M, Singhal H, Hall J, Li T, Holloran S, et al. Interferon-Stimulated Genes Are Transcriptionally Repressed by PR in Breast Cancer. Mol Cancer Res. 2017;15:1331-1340 pubmed publisher
immunoprecipitation; human; loading ...; fig 3a	Tikhanovich I, Zhao J, Bridges B, Kumer S, Roberts B, Weinman S. Arginine methylation regulates c-Myc-dependent transcription by altering promoter recruitment of the acetyltransferase p300. J Biol Chem. 2017;292:13333-13344 pubmed publisher
other; human; 1:500; loading ...; fig s9	He Y, Selvaraju S, Curtin M, Jakob C, Zhu H, Comess K, et al. The EED protein-protein interaction inhibitor A-395 inactivates the PRC2 complex. Nat Chem Biol. 2017;13:389-395 pubmed publisher
ChIP-Seq; human; loading ...; tbl 2 ChIP-Seq; mouse; loading ...; tbl 2	Busby M, Xue C, Li C, Farjoun Y, Gienger E, Yofe I, et al. Systematic comparison of monoclonal versus polyclonal antibodies for mapping histone modifications by ChIP-seq. Epigenetics Chromatin. 2016;9:49 pubmed
western blot; human; loading ...; fig 5a	Ono H, Basson M, Ito H. P300 inhibition enhances gemcitabine-induced apoptosis of pancreatic cancer. Oncotarget. 2016;7:51301-51310 pubmed publisher
ChIP-Seq; mouse; fig 3	Bandopadhayay P, Ramkissoon L, Jain P, Bergthold G, Wala J, Zeid R, et al. MYB-QKI rearrangements in angiocentric glioma drive tumorigenicity through a tripartite mechanism. Nat Genet. 2016;48:273-82 pubmed publisher
western blot; mouse; 1:1000; fig 6	Brina D, Miluzio A, Ricciardi S, Clarke K, Davidsen P, Viero G, et al. eIF6 coordinates insulin sensitivity and lipid metabolism by coupling translation to transcription. Nat Commun. 2015;6:8261 pubmed publisher
western blot; human; fig 5	Montgomery D, Sorum A, Guasch L, Nicklaus M, Meier J. Metabolic Regulation of Histone Acetyltransferases by Endogenous Acyl-CoA Cofactors. Chem Biol. 2015;22:1030-1039 pubmed publisher
immunocytochemistry; human	Luense S, Denner P, FernÃ¡ndez MontalvÃ¡n A, Hartung I, Husemann M, Stresemann C, et al. Quantification of histone H3 Lys27 trimethylation (H3K27me3) by high-throughput microscopy enables cellular large-scale screening for small-molecule EZH2 inhibitors. J Biomol Screen. 2015;20:190-201 pubmed publisher
chromatin immunoprecipitation; human; fig 5	Salz T, Deng C, Pampo C, Siemann D, Qiu Y, Brown K, et al. Histone Methyltransferase hSETD1A Is a Novel Regulator of Metastasis in Breast Cancer. Mol Cancer Res. 2015;13:461-9 pubmed publisher
	Hashwah H, Schmid C, Kasser S, Bertram K, Stelling A, Manz M, et al. Inactivation of CREBBP expands the germinal center B cell compartment, down-regulates MHCII expression and promotes DLBCL growth. Proc Natl Acad Sci U S A. 2017;114:9701-9706 pubmed publisher
	Kawazu M, Kojima S, Ueno T, Totoki Y, Nakamura H, Kunita A, et al. Integrative analysis of genomic alterations in triple-negative breast cancer in association with homologous recombination deficiency. PLoS Genet. 2017;13:e1006853 pubmed publisher
	Piunti A, Hashizume R, Morgan M, Bartom E, Horbinski C, Marshall S, et al. Therapeutic targeting of polycomb and BET bromodomain proteins in diffuse intrinsic pontine gliomas. Nat Med. 2017;23:493-500 pubmed publisher
	Rajarajacholan U, Thalappilly S, Riabowol K. ING1 regulates rRNA levels by altering nucleolar chromatin structure and mTOR localization. Nucleic Acids Res. 2017;45:1776-1792 pubmed publisher
	Gay G, Braun L, Brenier Pinchart M, Vollaire J, Josserand V, Bertini R, et al. Toxoplasma gondii TgIST co-opts host chromatin repressors dampening STAT1-dependent gene regulation and IFN-?-mediated host defenses. J Exp Med. 2016;213:1779-98 pubmed publisher
	Mitton B, Chae H, Hsu K, Dutta R, Aldana Masangkay G, Ferrari R, et al. Small molecule inhibition of cAMP response element binding protein in human acute myeloid leukemia cells. Leukemia. 2016;30:2302-2311 pubmed publisher
	Finegersh A, Ferguson C, Maxwell S, Mazariegos D, Farrell D, Homanics G. Repeated vapor ethanol exposure induces transient histone modifications in the brain that are modified by genotype and brain region. Front Mol Neurosci. 2015;8:39 pubmed publisher
	Robertson S, Lubick K, Freedman B, Carmody A, Best S. Tick-borne flaviviruses antagonize both IRF-1 and type I IFN signaling to inhibit dendritic cell function. J Immunol. 2014;192:2744-55 pubmed publisher

product information

SKU :

8173P

Product-Name :

Acetyl-Histone H3 (Lys27) (D5E4) XP® Rabbit mAb

Size :

40 ul

Price-(USD) :

135 USD

Species-x-Reactivity :

H, M, R, Mk, (Hm, X, Z, GP, Hr)

Applications :

Chromatin Immunoprecipitation

Product-Category :

Chromatin Regulation / Nuclear Function

Shipping-Temp :

AMBIENT

Storage-Temp :

-20°C

Product-Type :

Monoclonal Antibody

MW :

Host :

Rabbit

Target :

H3 (Lys28) acetylate

Primary-Protein :

Alt-Names :

H3 histone family, member A,H3/A,H31,H3FA,H3FB,H3FC,H3FD,H3FF,H3FH,H3FI,H3FJ,H3FK,H3FL,HIST1H3A,HIST1H3B,HIST1H3C,HIST1H3D,HIST1H3E,HIST1H3F,HIST1H3G,HIST1H3H,HIST1H3I,HIST1H3J,Histone H3.1,Histone H3/a,Histone H3/b,Histone H3/c,Histone H3/d,Histone H3/f,Histone H3/h,Histone H3/i,Histone H3/j,Histone H3/k,Histone H3/l,histone 1, H3a,histone cluster 1, H3a

more info or order :

Cell Signaling Technology product webpage