product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
K48-linkage Specific Polyubiquitin (D9D5) Rabbit mAb
catalog :
8081
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
D9D5
reactivity :
human, mouse, rat, brewer's yeast, fruit fly
application :
western blot, immunoprecipitation
citations: 26
Published Application/Species/Sample/DilutionReference
  • western blot; human; 1:1000; loading ...; fig 3b
Tian G, Hu C, Yun Y, Yang W, Dubiel W, Cheng Y, et al. Dual roles of HSP70 chaperone HSPA1 in quality control of nascent and newly synthesized proteins. EMBO J. 2021;40:e106183 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 3h
Ji M, Zhao Z, Li Y, Xu P, Shi J, Li Z, et al. FBXO6-mediated RNASET2 ubiquitination and degradation governs the development of ovarian cancer. Cell Death Dis. 2021;12:317 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 4c
Guo S, Chen Y, Yang Y, Zhang X, Ma L, Xue X, et al. TRIB2 modulates proteasome function to reduce ubiquitin stability and protect liver cancer cells against oxidative stress. Cell Death Dis. 2021;12:42 pubmed publisher
  • western blot; mouse; loading ...; fig 6f
Wang W, Hu D, Wu C, Feng Y, Li A, Liu W, et al. STING promotes NLRP3 localization in ER and facilitates NLRP3 deubiquitination to activate the inflammasome upon HSV-1 infection. PLoS Pathog. 2020;16:e1008335 pubmed publisher
  • western blot; human; loading ...; fig 6c
Gain C, Malik S, Bhattacharjee S, Ghosh A, Robertson E, Das B, et al. Proteasomal inhibition triggers viral oncoprotein degradation via autophagy-lysosomal pathway. PLoS Pathog. 2020;16:e1008105 pubmed publisher
  • western blot; human; loading ...; fig 6b
Luo H, Jing B, Xia Y, Zhang Y, Hu M, Cai H, et al. WP1130 reveals USP24 as a novel target in T-cell acute lymphoblastic leukemia. Cancer Cell Int. 2019;19:56 pubmed publisher
  • immunoprecipitation; mouse; 1:1000; loading ...; fig 5c
Panda S, Gekara N. The deubiquitinase MYSM1 dampens NOD2-mediated inflammation and tissue damage by inactivating the RIP2 complex. Nat Commun. 2018;9:4654 pubmed publisher
  • immunoprecipitation; human; loading ...; fig 3b
Glaeser K, Urban M, Fenech E, Voloshanenko O, Kranz D, Lari F, et al. ERAD-dependent control of the Wnt secretory factor Evi. EMBO J. 2018;37: pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 6c
Korczeniewska J, Barnes B. Corrected and Republished from: The COP9 Signalosome Interacts with and Regulates Interferon Regulatory Factor 5 Protein Stability. Mol Cell Biol. 2018;38: pubmed publisher
  • western blot; human; loading ...; fig 2d
Ashkenazi A, Bento C, Ricketts T, Vicinanza M, Siddiqi F, Pavel M, et al. Polyglutamine tracts regulate beclin 1-dependent autophagy. Nature. 2017;545:108-111 pubmed publisher
  • western blot; fruit fly ; loading ...; fig 4e
Vonk J, Yeshaw W, Pinto F, Faber A, Lahaye L, Kanon B, et al. Drosophila Vps13 Is Required for Protein Homeostasis in the Brain. PLoS ONE. 2017;12:e0170106 pubmed publisher
  • western blot; brewer's yeast; 1:1000; loading ...; fig 4a
Fang N, Zhu M, Rose A, Wu K, Mayor T. Deubiquitinase activity is required for the proteasomal degradation of misfolded cytosolic proteins upon heat-stress. Nat Commun. 2016;7:12907 pubmed publisher
  • immunoprecipitation; mouse; loading ...; fig 1c,1d
Zhu L, Luo T, Xu X, Guo Y, Zhao X, Wang T, et al. E3 ubiquitin ligase Cbl-b negatively regulates C-type lectin receptor-mediated antifungal innate immunity. J Exp Med. 2016;213:1555-70 pubmed publisher
  • western blot; mouse; loading ...
Yu Z, Chen T, Li X, Yang M, Tang S, Zhu X, et al. Lys29-linkage of ASK1 by Skp1-Cullin 1-Fbxo21 ubiquitin ligase complex is required for antiviral innate response. elife. 2016;5: pubmed publisher
  • western blot; mouse; fig 5
Su X, Yan H, Huang Y, Yun H, Zeng B, Wang E, et al. Expression of FABP4, adipsin and adiponectin in Paneth cells is modulated by gut Lactobacillus. Sci Rep. 2015;5:18588 pubmed publisher
  • western blot; human; loading ...; fig 2c
Zhao J, Molitor T, Langston J, Nichols R. LRRK2 dephosphorylation increases its ubiquitination. Biochem J. 2015;469:107-20 pubmed publisher
  • western blot; mouse; fig 7
Yazlovitskaya E, Tseng H, Viquez O, Tu T, Mernaugh G, McKee K, et al. Integrin α3β1 regulates kidney collecting duct development via TRAF6-dependent K63-linked polyubiquitination of Akt. Mol Biol Cell. 2015;26:1857-74 pubmed publisher
  • western blot; rat
Opperman C, Sishi B. Tumor necrosis factor alpha stimulates p62 accumulation and enhances proteasome activity independently of ROS. Cell Biol Toxicol. 2015;31:83-94 pubmed publisher
  • western blot; rat; loading ...; fig s4
Widagdo J, Chai Y, Ridder M, Chau Y, Johnson R, Sah P, et al. Activity-Dependent Ubiquitination of GluA1 and GluA2 Regulates AMPA Receptor Intracellular Sorting and Degradation. Cell Rep. 2015;10:783-795 pubmed publisher
  • western blot; mouse; 1:1,000
Shiba Fukushima K, Inoshita T, Hattori N, Imai Y. Lysine 63-linked polyubiquitination is dispensable for Parkin-mediated mitophagy. J Biol Chem. 2014;289:33131-6 pubmed publisher
  • western blot; mouse
Stevenson C, de la Rosa G, Anderson C, Murphy P, Capece T, Kim M, et al. Essential role of Elmo1 in Dock2-dependent lymphocyte migration. J Immunol. 2014;192:6062-70 pubmed publisher
Hooper C, Jackson S, Coughlin E, Coon J, Miyamoto S. Covalent modification of the NF-κB essential modulator (NEMO) by a chemical compound can regulate its ubiquitin binding properties in vitro. J Biol Chem. 2014;289:33161-74 pubmed publisher
Liu N, Li X, Huang H, Zhao C, Liao S, Yang C, et al. Clinically used antirheumatic agent auranofin is a proteasomal deubiquitinase inhibitor and inhibits tumor growth. Oncotarget. 2014;5:5453-71 pubmed
Blessing N, Brockman A, Chadee D. The E3 ligase CHIP mediates ubiquitination and degradation of mixed-lineage kinase 3. Mol Cell Biol. 2014;34:3132-43 pubmed publisher
Trang V, Valkevich E, Minami S, Chen Y, Ge Y, Strieter E. Nonenzymatic polymerization of ubiquitin: single-step synthesis and isolation of discrete ubiquitin oligomers. Angew Chem Int Ed Engl. 2012;51:13085-8 pubmed publisher
Lai A, Durrant M, Zuo D, Ratcliffe C, Park M. Met kinase-dependent loss of the E3 ligase Cbl in gastric cancer. J Biol Chem. 2012;287:8048-59 pubmed publisher
product information
SKU :
8081S
Product-Name :
K48-linkage Specific Polyubiquitin (D9D5) Rabbit mAb
Size :
100 ul
Price-(USD) :
274 USD
Species-x-Reactivity :
All
Applications :
Western blot
Product-Category :
Ubiquitin and Ubiquitin-like proteins
Shipping-Temp :
COLD
Storage-Temp :
-20°C
Product-Type :
Monoclonal Antibody
Host :
Rabbit
Target :
Ubiquitin poly Lys48
Primary-Protein :
ubiquitin
Alt-Names :
40S ribosomal protein S27a,CEP80,RPS27A,Ribosomal protein S27a,UBA52,UBA80,UBB,UBC,UBCEP1,UBCEP80,UBIQ,ubiquitin and ribosomal protein S27a,ubiquitin carboxyl extension protein 80,ubiquitin-CEP80
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
https://www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.