product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
SQSTM1/p62 (D5E2) Rabbit mAb
catalog :
8025
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
D5E2
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 23
Published Application/Species/Sample/DilutionReference
  • western blot; rat; loading ...; fig 2f
Xie X, Bi H, Lai S, Zhang Y, Li N, Cao H, et al. The immunoproteasome catalytic β5i subunit regulates cardiac hypertrophy by targeting the autophagy protein ATG5 for degradation. Sci Adv. 2019;5:eaau0495 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 2e
Kwon D, Park O, Kim L, Jung Y, Park Y, Jeong H, et al. Insights into degradation mechanism of N-end rule substrates by p62/SQSTM1 autophagy adapter. Nat Commun. 2018;9:3291 pubmed publisher
  • western blot; human; loading ...; fig 2a
Ni Z, HE J, Wu Y, Hu C, Dai X, Yan X, et al. AKT-mediated phosphorylation of ATG4B impairs mitochondrial activity and enhances the Warburg effect in hepatocellular carcinoma cells. Autophagy. 2018;14:685-701 pubmed publisher
  • western blot; human; 1:500; loading ...; fig 3c
Vidoni C, Secomandi E, Castiglioni A, Melone M, Isidoro C. Resveratrol protects neuronal-like cells expressing mutant Huntingtin from dopamine toxicity by rescuing ATG4-mediated autophagosome formation. Neurochem Int. 2018;117:174-187 pubmed publisher
  • western blot; human; loading ...; fig 2
Zhou Y, Huang N, Wu J, Zhen N, Li N, Li Y, et al. Silencing of NRAGE induces autophagy via AMPK/Ulk1/Atg13 signaling pathway in NSCLC cells. Tumour Biol. 2017;39:1010428317709676 pubmed publisher
  • western blot; human; loading ...; fig 2b
Zhang X, Fan J, Wang S, Li Y, Wang Y, Li S, et al. Targeting CD47 and Autophagy Elicited Enhanced Antitumor Effects in Non-Small Cell Lung Cancer. Cancer Immunol Res. 2017;5:363-375 pubmed publisher
  • western blot; human; loading ...; fig 8f
Marwaha R, Arya S, Jagga D, Kaur H, Tuli A, Sharma M. The Rab7 effector PLEKHM1 binds Arl8b to promote cargo traffic to lysosomes. J Cell Biol. 2017;216:1051-1070 pubmed publisher
  • western blot; mouse; 1:1000; fig 3a
Dai S, Wang B, Li W, Wang L, Song X, Guo C, et al. Systemic application of 3-methyladenine markedly inhibited atherosclerotic lesion in ApoE-/- mice by modulating autophagy, foam cell formation and immune-negative molecules. Cell Death Dis. 2016;7:e2498 pubmed publisher
  • western blot; human; loading ...; fig 1h
Li D, Xie B, Wu X, Li J, Ding Y, Wen X, et al. Late-stage inhibition of autophagy enhances calreticulin surface exposure. Oncotarget. 2016;7:80842-80854 pubmed publisher
  • western blot; human; 1:1000; fig 7b
Grinshtein N, Rioseco C, Marcellus R, UEHLING D, Aman A, Lun X, et al. Small molecule epigenetic screen identifies novel EZH2 and HDAC inhibitors that target glioblastoma brain tumor-initiating cells. Oncotarget. 2016;7:59360-59376 pubmed publisher
  • western blot; human; 1:1000; fig 3
Yu L, Wu W, Gu C, Zhong D, Zhao X, Kong Y, et al. Obatoclax impairs lysosomal function to block autophagy in cisplatin-sensitive and -resistant esophageal cancer cells. Oncotarget. 2016;7:14693-707 pubmed publisher
  • immunoprecipitation; human; fig 4
  • western blot; human; 1:1000; fig 3
Chen K, Zeng J, Xiao H, Huang C, Hu J, Yao W, et al. Regulation of glucose metabolism by p62/SQSTM1 through HIF1α. J Cell Sci. 2016;129:817-30 pubmed publisher
  • western blot; human; 1:1000; fig 1e
Metge B, Mitra A, Chen D, Shevde L, Samant R. N-Myc and STAT Interactor regulates autophagy and chemosensitivity in breast cancer cells. Sci Rep. 2015;5:11995 pubmed publisher
  • western blot; human; fig 4
Hotokezaka Y, Katayama I, van Leyen K, Nakamura T. GSK-3β-dependent downregulation of γ-taxilin and αNAC merge to regulate ER stress responses. Cell Death Dis. 2015;6:e1719 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 5
Chuang W, Su C, Lin P, Lin C, Chen Y. Sann-Joong-Kuey-Jian-Tang induces autophagy in HepG2 cells via regulation of the phosphoinositide-3 kinase/Akt/mammalian target of rapamycin and p38 mitogen-activated protein kinase pathways. Mol Med Rep. 2015;12:1677-84 pubmed publisher
  • western blot; mouse; fig 4c
  • immunohistochemistry - paraffin section; human; fig 5a
Mir S, George N, Zahoor L, Harms R, Guinn Z, SARVETNICK N. Inhibition of autophagic turnover in β-cells by fatty acids and glucose leads to apoptotic cell death. J Biol Chem. 2015;290:6071-85 pubmed publisher
Zhang P, Zheng Z, Ling L, Yang X, Zhang N, Wang X, et al. w09, a novel autophagy enhancer, induces autophagy-dependent cell apoptosis via activation of the EGFR-mediated RAS-RAF1-MAP2K-MAPK1/3 pathway. Autophagy. 2017;13:1093-1112 pubmed publisher
Rovithi M, de Haas R, Honeywell R, Poel D, Peters G, Griffioen A, et al. Alternative scheduling of pulsatile, high dose sunitinib efficiently suppresses tumor growth. J Exp Clin Cancer Res. 2016;35:138 pubmed publisher
Ji M, Feng Q, He G, Yang L, Tang W, Lao X, et al. Silencing homeobox C6 inhibits colorectal cancer cell proliferation. Oncotarget. 2016;7:29216-27 pubmed publisher
Zhang C, Jiang Y, Zhang J, Huang J, Wang J. 8-p-Hdroxybenzoyl Tovarol Induces Paraptosis Like Cell Death and Protective Autophagy in Human Cervical Cancer HeLa Cells. Int J Mol Sci. 2015;16:14979-96 pubmed publisher
Bernard M, Dieudé M, Yang B, Hamelin K, Underwood K, Hébert M. Autophagy fosters myofibroblast differentiation through MTORC2 activation and downstream upregulation of CTGF. Autophagy. 2014;10:2193-207 pubmed publisher
Tripathi R, Ash D, Shaha C. Beclin-1-p53 interaction is crucial for cell fate determination in embryonal carcinoma cells. J Cell Mol Med. 2014;18:2275-86 pubmed publisher
Xu L, Wang Z, Xu D, Lin G, Li D, Wan T, et al. Expression of Derlin-1 and its effect on expression of autophagy marker genes under endoplasmic reticulum stress in lung cancer cells. Cancer Cell Int. 2014;14:50 pubmed publisher
product information
SKU :
8025S
Product-Name :
SQSTM1/p62 (D5E2) Rabbit mAb
Size :
100 ul
Price-(USD) :
235 USD
Species-x-Reactivity :
H, Mk
Applications :
Immunoprecipitation
Product-Category :
Autophagy
Shipping-Temp :
AMBIENT
Storage-Temp :
-20°C
Product-Type :
Monoclonal Antibody
MW :
62
Host :
Rabbit
Target :
SQSTM1
Primary-Protein :
SQSTM1
Alt-Names :
A170,EBI3-associated protein of 60 kDa,EBI3-associated protein p60,EBIAP,ORCA,OSIL,PDB3,Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa,SQSTM,SQSTM1,Sequestosome-1,Ubiquitin-binding protein p62,ZIP3,oxidative stress induced like,p60,p62,p62B,phosphotyrosine independent ligand for the Lck SH2 domain p62,sequestosome 1
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
https://www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.