product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
NF-κB p65 (L8F6) Mouse mAb
catalog :
6956
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
L8F6
reactivity :
human, mouse, rat, dogs, bovine, pigs
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section, proximity ligation assay, western blot knockout validation
citations: 40
Published Application/Species/Sample/DilutionReference
  • western blot knockout validation; mouse; 1:1000; loading ...; fig e2f
El Brolosy M, Kontarakis Z, Rossi A, Kuenne C, Günther S, Fukuda N, et al. Genetic compensation triggered by mutant mRNA degradation. Nature. 2019;: pubmed publisher
  • western blot; mouse; fig 5i
Ma S, Xu H, Huang W, Gao Y, Zhou H, Li X, et al. Chrysophanol Relieves Cisplatin-Induced Nephrotoxicity via Concomitant Inhibition of Oxidative Stress, Apoptosis, and Inflammation. Front Physiol. 2021;12:706359 pubmed publisher
  • immunocytochemistry; human; 1:200; loading ...; fig ev3k
Du Y, Zhang M, Liu X, Li Z, Hu M, Tian Y, et al. CDC20 promotes bone formation via APC/C dependent ubiquitination and degradation of p65. EMBO Rep. 2021;22:e52576 pubmed publisher
  • western blot; mouse; 1:500; loading ...; fig 1e
  • western blot; human; loading ...; fig 2a
Zhang Z, Zhang L, Zhang Q, Liu B, Li F, Xin Y, et al. HO-1/CO Maintains Intestinal Barrier Integrity through NF-κB/MLCK Pathway in Intestinal HO-1-/- Mice. Oxid Med Cell Longev. 2021;2021:6620873 pubmed publisher
  • western blot; rat; 1:1000; loading ...; fig 6a
Zhang F, Wang Y, Liu P, Di P, Li M, Wang C. Puerarin exhibits antiinflammatory properties in gunpowder smog-induced acute lung injury in rats via regulation of the renin-angiotensin system and the NFκB signaling pathway. Exp Ther Med. 2021;22:809 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 6
Zhang S, Qu J, Wang L, Li M, Xu D, Zhao Y, et al. Activation of Toll-Like Receptor 7 Signaling Pathway in Primary Sjögren's Syndrome-Associated Thrombocytopenia. Front Immunol. 2021;12:637659 pubmed publisher
  • western blot; mouse; loading ...; fig 4f
Zhang D, Liu C, Li H, Jiao J. Deficiency of STING Signaling in Embryonic Cerebral Cortex Leads to Neurogenic Abnormalities and Autistic-Like Behaviors. Adv Sci (Weinh). 2020;7:2002117 pubmed publisher
  • western blot; human; fig 1b
  • western blot; mouse; loading ...; fig 1a
Chen M, Zhao Z, Meng Q, Liang P, Su Z, Wu Y, et al. TRIM14 Promotes Noncanonical NF-κB Activation by Modulating p100/p52 Stability via Selective Autophagy. Adv Sci (Weinh). 2020;7:1901261 pubmed publisher
  • western blot; human; loading ...; fig 2c
Bonacini M, Negri A, Davalli P, Naponelli V, Ramazzina I, Lenzi C, et al. Clusterin Silencing in Prostate Cancer Induces Matrix Metalloproteinases by an NF-κB-Dependent Mechanism. J Oncol. 2019;2019:4081624 pubmed publisher
  • immunocytochemistry; human; 1:800; loading ...; fig 3d
Jin Y, Li Y, Wang X, Yang Y. Secretory leukocyte protease inhibitor suppresses HPV E6-expressing HNSCC progression by mediating NF-κB and Akt pathways. Cancer Cell Int. 2019;19:220 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 5b
Fiore A, Ugel S, De Sanctis F, Sandri S, Fracasso G, Trovato R, et al. Induction of immunosuppressive functions and NF-κB by FLIP in monocytes. Nat Commun. 2018;9:5193 pubmed publisher
  • western blot; mouse; loading ...; fig 1e
  • western blot; human; loading ...; fig 1j
Mouhadeb O, Ben Shlomo S, Cohen K, Farkash I, Gruber S, Maharshak N, et al. Impaired COMMD10-Mediated Regulation of Ly6Chi Monocyte-Driven Inflammation Disrupts Gut Barrier Function. Front Immunol. 2018;9:2623 pubmed publisher
  • proximity ligation assay; human; loading ...; fig 4a
Turner J, Kashyap T, Dawson J, Gomez J, Bauer A, Grant S, et al. XPO1 inhibitor combination therapy with bortezomib or carfilzomib induces nuclear localization of IκBα and overcomes acquired proteasome inhibitor resistance in human multiple myeloma. Oncotarget. 2016;7:78896-78909 pubmed publisher
  • western blot; human; fig 5
Li Q, Karim A, Ding X, Das B, Dobrowolski C, Gibson R, et al. Novel high throughput pooled shRNA screening identifies NQO1 as a potential drug target for host directed therapy for tuberculosis. Sci Rep. 2016;6:27566 pubmed publisher
  • western blot; mouse; fig 1
Tortola L, Nitsch R, Bertrand M, Kogler M, Redouane Y, Kozieradzki I, et al. The Tumor Suppressor Hace1 Is a Critical Regulator of TNFR1-Mediated Cell Fate. Cell Rep. 2016;15:1481-1492 pubmed publisher
  • western blot; pigs ; fig 6
Ren W, Yin J, Chen S, Duan J, Liu G, Li T, et al. Proteome analysis for the global proteins in the jejunum tissues of enterotoxigenic Escherichia coli -infected piglets. Sci Rep. 2016;6:25640 pubmed publisher
  • western blot; human; loading ...; fig 4a
Wang Y, Cao J, Fan Y, Xie Y, Xu Z, Yin Z, et al. Artemisinin inhibits monocyte adhesion to HUVECs through the NF-?B and MAPK pathways in vitro. Int J Mol Med. 2016;37:1567-75 pubmed publisher
  • western blot; mouse; fig 4e
Yu Z, Chen T, Li X, Yang M, Tang S, Zhu X, et al. Lys29-linkage of ASK1 by Skp1-Cullin 1-Fbxo21 ubiquitin ligase complex is required for antiviral innate response. elife. 2016;5: pubmed publisher
  • immunoprecipitation; mouse; 1:100; loading ...; fig 7c
Leus N, van der Wouden P, van den Bosch T, Hooghiemstra W, Ourailidou M, Kistemaker L, et al. HDAC 3-selective inhibitor RGFP966 demonstrates anti-inflammatory properties in RAW 264.7 macrophages and mouse precision-cut lung slices by attenuating NF-κB p65 transcriptional activity. Biochem Pharmacol. 2016;108:58-74 pubmed publisher
  • western blot; mouse; fig 2b
Liu H, Shi H, Huang F, Peterson K, Wu H, Lan Y, et al. Astragaloside IV inhibits microglia activation via glucocorticoid receptor mediated signaling pathway. Sci Rep. 2016;6:19137 pubmed publisher
  • western blot; human; 1:1000; fig 3
Chen K, Zeng J, Xiao H, Huang C, Hu J, Yao W, et al. Regulation of glucose metabolism by p62/SQSTM1 through HIF1α. J Cell Sci. 2016;129:817-30 pubmed publisher
  • western blot; mouse; 1:500
He J, Quintana M, Sullivan J, L Parry T, J Grevengoed T, Schisler J, et al. MuRF2 regulates PPARγ1 activity to protect against diabetic cardiomyopathy and enhance weight gain induced by a high fat diet. Cardiovasc Diabetol. 2015;14:97 pubmed publisher
  • western blot; human; 1:1000
Zhong X, Liao Y, Chen L, Liu G, Feng Y, Zeng T, et al. The MicroRNAs in the Pathogenesis of Metabolic Memory. Endocrinology. 2015;156:3157-68 pubmed publisher
  • western blot; human; fig f4
Yang L, Zhang S, George S, Teng R, You X, Xu M, et al. Targeting Notch1 and proteasome as an effective strategy to suppress T-cell lymphoproliferative neoplasms. Oncotarget. 2015;6:14953-69 pubmed
  • immunohistochemistry - paraffin section; human
  • western blot; human; fig 4
Cang D, Guo K, Zhao F. Dendritic cells enhance UHMWPE wear particle-induced osteoclast differentiation of macrophages. J Biomed Mater Res A. 2015;103:3349-54 pubmed publisher
  • western blot; mouse
Kim S, Nam S, Friedman M. The Tomato Glycoalkaloid α-Tomatine Induces Caspase-Independent Cell Death in Mouse Colon Cancer CT-26 Cells and Transplanted Tumors in Mice. J Agric Food Chem. 2015;63:1142-1150 pubmed publisher
  • western blot; mouse
Ren W, Duan J, Yin J, Liu G, Cao Z, Xiong X, et al. Dietary L-glutamine supplementation modulates microbial community and activates innate immunity in the mouse intestine. Amino Acids. 2014;46:2403-13 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 5a
Piao H, Yuan Y, Wang M, Sun Y, Liang H, Ma L. ?-catenin acts as a tumour suppressor in E-cadherin-negative basal-like breast cancer by inhibiting NF-?B signalling. Nat Cell Biol. 2014;16:245-54 pubmed publisher
  • western blot; human; 1:500; fig s4
Datta De D, Datta A, Bhattacharjya S, Roychoudhury S. NF-kappaB mediated transcriptional repression of acid modifying hormone gastrin. PLoS ONE. 2013;8:e73409 pubmed publisher
Shen Y, Qin H, Chen J, Mou L, He Y, Yan Y, et al. Postnatal activation of TLR4 in astrocytes promotes excitatory synaptogenesis in hippocampal neurons. J Cell Biol. 2016;215:719-734 pubmed
Schliesser M, Claus R, Hielscher T, Grimm C, Weichenhan D, Blaes J, et al. Prognostic relevance of miRNA-155 methylation in anaplastic glioma. Oncotarget. 2016;7:82028-82045 pubmed publisher
Zhan J, Qin W, Zhang Y, Jiang J, Ma H, Li Q, et al. Upregulation of neuronal zinc finger protein A20 expression is required for electroacupuncture to attenuate the cerebral inflammatory injury mediated by the nuclear factor-kB signaling pathway in cerebral ischemia/reperfusion rats. J Neuroinflammation. 2016;13:258 pubmed
Zhang H, Li L, Wang Y, Dong F, Chen X, Liu F, et al. NF-?B signaling maintains the survival of cadmium-exposed human renal glomerular endothelial cells. Int J Mol Med. 2016;38:417-22 pubmed publisher
Zhang L, Wang J, Liang R, Huang S, Xu J, Yuan L, et al. RASSF8 downregulation promotes lymphangiogenesis and metastasis in esophageal squamous cell carcinoma. Oncotarget. 2015;6:34510-24 pubmed publisher
Yang F, Wang H, Jiang Z, Hu A, Chu L, Sun Y, et al. MicroRNA-19a mediates gastric carcinoma cell proliferation through the activation of nuclear factor-κB. Mol Med Rep. 2015;12:5780-6 pubmed publisher
Rades D, Huttenlocher S, Seibold N, Gebhard M, Thorns C, Hasselbacher K, et al. Nuclear expression of p65 (RelA) in patients receiving post-operative radiotherapy for locally advanced squamous cell carcinoma of the head and neck. BMC Cancer. 2015;15:102 pubmed publisher
Li J, Xie C, Zhuang J, Li H, Yao Y, Shao C, et al. Resveratrol attenuates inflammation in the rat heart subjected to ischemia-reperfusion: Role of the TLR4/NF-κB signaling pathway. Mol Med Rep. 2015;11:1120-6 pubmed publisher
Liu N, Li X, Huang H, Zhao C, Liao S, Yang C, et al. Clinically used antirheumatic agent auranofin is a proteasomal deubiquitinase inhibitor and inhibits tumor growth. Oncotarget. 2014;5:5453-71 pubmed
Oizumi A, Nakayama H, Okino N, Iwahara C, Kina K, Matsumoto R, et al. Pseudomonas-derived ceramidase induces production of inflammatory mediators from human keratinocytes via sphingosine-1-phosphate. PLoS ONE. 2014;9:e89402 pubmed publisher
Hu L, Bray M, Geng Y, Kopecko D. Campylobacter jejuni-mediated induction of CC and CXC chemokines and chemokine receptors in human dendritic cells. Infect Immun. 2012;80:2929-39 pubmed publisher
product information
SKU :
6956S
Product-Name :
NF-κB p65 (L8F6) Mouse mAb
Size :
100 ul
Price-(USD) :
235 USD
Species-x-Reactivity :
H, M, R, Hm, Mk, Mi, B, Dg, Pg
Applications :
Chromatin Immunoprecipitation
Product-Category :
Immunology and Inflammation
Shipping-Temp :
AMBIENT
Storage-Temp :
-20°C
Product-Type :
Monoclonal Antibody
MW :
65
Host :
Mouse
Target :
NFkB-p65
Primary-Protein :
NFkB-p65
Alt-Names :
MGC131774,NFKB3,Nuclear factor NF-kappa-B p65 subunit,Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3,RELA,TF65,Transcription factor p65,p65,v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 (p65)),v-rel reticuloendotheliosis viral oncogene homolog A (avian),v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, p65
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
https://www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.