product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
Smad1 (D59D7) XP® Rabbit mAb
catalog :
6944
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
D59D7
reactivity :
human, mouse, bovine, Xenopus laevis
application :
western blot, immunocytochemistry, immunoprecipitation, flow cytometry, chromatin immunoprecipitation
citations: 36
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; loading ...; fig 5f
Lee A, Pingali S, Pinilla Ibarz J, Atchison M, Koumenis C, Argon Y, et al. Loss of AID exacerbates the malignant progression of CLL. Leukemia. 2022;36:2430-2442 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig s1a
  • western blot; human; 1:1000; loading ...; fig 1b
Forouhan M, Lim W, Zanetti Domingues L, Tynan C, Roberts T, Malik B, et al. AR cooperates with SMAD4 to maintain skeletal muscle homeostasis. Acta Neuropathol. 2022;143:713-731 pubmed publisher
  • western blot; mouse; 1:1000; fig 1c, s5a, s5d
Yang J, Kitami M, Pan H, Nakamura M, Zhang H, Liu F, et al. Augmented BMP signaling commits cranial neural crest cells to a chondrogenic fate by suppressing autophagic β-catenin degradation. Sci Signal. 2021;14: pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 6c
Kushioka J, Kaito T, Okada R, Ishiguro H, Bal Z, Kodama J, et al. A novel negative regulatory mechanism of Smurf2 in BMP/Smad signaling in bone. Bone Res. 2020;8:41 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 5a
Li M, Xie Z, Li J, Lin J, Zheng G, Liu W, et al. GAS5 protects against osteoporosis by targeting UPF1/SMAD7 axis in osteoblast differentiation. elife. 2020;9: pubmed publisher
  • western blot; human; loading ...; fig 3c
Park N, Kang H. BMP-Induced MicroRNA-101 Expression Regulates Vascular Smooth Muscle Cell Migration. Int J Mol Sci. 2020;21: pubmed publisher
  • western blot; human; loading ...; fig s2
Shoemaker L, McCormick A, Allen B, Chang S. Evidence for endothelial-to-mesenchymal transition in human brain arteriovenous malformations. Clin Transl Med. 2020;10:e99 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 4b
Li P, Fleischhauer L, Nicolae C, Prein C, Farkas Z, Saller M, et al. Mice Lacking the Matrilin Family of Extracellular Matrix Proteins Develop Mild Skeletal Abnormalities and Are Susceptible to Age-Associated Osteoarthritis. Int J Mol Sci. 2020;21: pubmed publisher
  • western blot; mouse; loading ...; fig 3a
Fan Q, He W, Gayen M, Benoit M, Luo X, Hu X, et al. Activated CX3CL1/Smad2 Signals Prevent Neuronal Loss and Alzheimer's Tau Pathology-Mediated Cognitive Dysfunction. J Neurosci. 2020;40:1133-1144 pubmed publisher
  • chromatin immunoprecipitation; human; loading ...; fig 3g
  • western blot; human; 1:1000; loading ...; fig 1e
Hiepen C, Jatzlau J, Hildebrandt S, Kampfrath B, Goktas M, Murgai A, et al. BMPR2 acts as a gatekeeper to protect endothelial cells from increased TGFβ responses and altered cell mechanics. PLoS Biol. 2019;17:e3000557 pubmed publisher
  • western blot; human; 1:250; loading ...; fig 2b
Gorrell R, Totten M, Schoerning L, Newby J, Geyman L, Lawless W, et al. Identification of a bone morphogenetic protein type 2 receptor neutralizing antibody. BMC Res Notes. 2019;12:331 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 4e
Tsai C, Tsai C, Yi J, Kao H, Huang Y, Wang C, et al. Activin A regulates the epidermal growth factor receptor promoter by activating the PI3K/SP1 pathway in oral squamous cell carcinoma cells. Sci Rep. 2019;9:5197 pubmed publisher
  • western blot; mouse; loading ...; fig 6c
Hendrikx S, Coso S, Prat Luri B, Wetterwald L, Sabine A, Franco C, et al. Endothelial Calcineurin Signaling Restrains Metastatic Outgrowth by Regulating Bmp2. Cell Rep. 2019;26:1227-1241.e6 pubmed publisher
  • western blot; human; 1:2000; fig 2c
Bajikar S, Wang C, Borten M, Pereira E, Atkins K, Janes K. Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor Sequestration in Triple-Negative Breast Cancer. Dev Cell. 2017;43:418-435.e13 pubmed publisher
  • western blot; human; loading ...; fig 6d
Sun J, Liu X, Gao H, Zhang L, Ji Q, Wang Z, et al. Overexpression of colorectal cancer oncogene CHRDL2 predicts a poor prognosis. Oncotarget. 2017;8:11489-11506 pubmed publisher
  • western blot; human; 1:20,000; loading ...; fig S1i
Mouillesseaux K, Wiley D, Saunders L, Wylie L, Kushner E, Chong D, et al. Notch regulates BMP responsiveness and lateral branching in vessel networks via SMAD6. Nat Commun. 2016;7:13247 pubmed publisher
  • western blot; human; 1:1000; fig st1
Treindl F, Ruprecht B, Beiter Y, Schultz S, Döttinger A, Staebler A, et al. A bead-based western for high-throughput cellular signal transduction analyses. Nat Commun. 2016;7:12852 pubmed publisher
  • western blot; mouse; 1:1000; fig 3
West J, Carrier E, Bloodworth N, Schroer A, Chen P, Ryzhova L, et al. Serotonin 2B Receptor Antagonism Prevents Heritable Pulmonary Arterial Hypertension. PLoS ONE. 2016;11:e0148657 pubmed publisher
  • immunocytochemistry; mouse; loading ...; fig 1c
  • western blot; mouse; loading ...; fig 4c
Marks Bluth J, Khanna A, Chandrakanthan V, Thoms J, Bee T, Eich C, et al. SMAD1 and SMAD5 Expression Is Coordinately Regulated by FLI1 and GATA2 during Endothelial Development. Mol Cell Biol. 2015;35:2165-72 pubmed publisher
  • western blot; human
Perna F, Vu L, Themeli M, Kriks S, Hoya Arias R, Khanin R, et al. The polycomb group protein L3MBTL1 represses a SMAD5-mediated hematopoietic transcriptional program in human pluripotent stem cells. Stem Cell Reports. 2015;4:658-69 pubmed publisher
  • western blot; mouse; 1:1000
Tian E, Stevens S, Guan Y, Springer D, Anderson S, Starost M, et al. Galnt1 is required for normal heart valve development and cardiac function. PLoS ONE. 2015;10:e0115861 pubmed publisher
  • western blot; human; loading ...; fig s3g
Li Q, Zou J, Wang M, Ding X, Chepelev I, Zhou X, et al. Critical role of histone demethylase Jmjd3 in the regulation of CD4+ T-cell differentiation. Nat Commun. 2014;5:5780 pubmed publisher
  • chromatin immunoprecipitation; mouse; fig 4
Onishi K, Tonge P, Nagy A, Zandstra P. Local BMP-SMAD1 signaling increases LIF receptor-dependent STAT3 responsiveness and primed-to-naive mouse pluripotent stem cell conversion frequency. Stem Cell Reports. 2014;3:156-68 pubmed publisher
  • western blot; human; 1:1000
Hou P, Chuang C, Kao C, Chou S, Stone L, Ho H, et al. LHX2 regulates the neural differentiation of human embryonic stem cells via transcriptional modulation of PAX6 and CER1. Nucleic Acids Res. 2013;41:7753-70 pubmed publisher
  • western blot; human; 1:2000
Ren G, Beech C, Smas C. The immunoglobulin superfamily protein differentiation of embryonic stem cells 1 (dies1) has a regulatory role in preadipocyte to adipocyte conversion. PLoS ONE. 2013;8:e65531 pubmed publisher
  • western blot; Xenopus laevis
Lim C, Reversade B, Knowles B, Solter D. Optimal histone H3 to linker histone H1 chromatin ratio is vital for mesodermal competence in Xenopus. Development. 2013;140:853-60 pubmed publisher
Arai H, Sato F, Yamamoto T, Woltjen K, Kiyonari H, Yoshimoto Y, et al. Metalloprotease-Dependent Attenuation of BMP Signaling Restricts Cardiac Neural Crest Cell Fate. Cell Rep. 2019;29:603-616.e5 pubmed publisher
Tang Y, Zheng L, Zhou J, Chen Y, Yang L, Deng F, et al. miR‑203‑3p participates in the suppression of diabetes‑associated osteogenesis in the jaw bone through targeting Smad1. Int J Mol Med. 2018;41:1595-1607 pubmed publisher
Tumelty K, Higginson Scott N, Fan X, Bajaj P, Knowlton K, Shamashkin M, et al. Identification of direct negative cross-talk between the SLIT2 and bone morphogenetic protein-Gremlin signaling pathways. J Biol Chem. 2018;293:3039-3055 pubmed publisher
Bui A, Huang M, Havard M, Laurent Tchenio F, Dautry F, Tchenio T. Transient exposure to androgens induces a remarkable self-sustained quiescent state in dispersed prostate cancer cells. Cell Cycle. 2017;16:879-893 pubmed publisher
Qi Z, Li Y, Zhao B, Xu C, Liu Y, Li H, et al. BMP restricts stemness of intestinal Lgr5+ stem cells by directly suppressing their signature genes. Nat Commun. 2017;8:13824 pubmed publisher
Okamoto A, Nojiri T, Konishi K, Tokudome T, Miura K, Hosoda H, et al. Atrial natriuretic peptide protects against bleomycin-induced pulmonary fibrosis via vascular endothelial cells in mice : ANP for pulmonary fibrosis. Respir Res. 2017;18:1 pubmed publisher
Coster A, Thorne C, Wu L, Altschuler S. Examining Crosstalk among Transforming Growth Factor β, Bone Morphogenetic Protein, and Wnt Pathways. J Biol Chem. 2017;292:244-250 pubmed publisher
Klatte Schulz F, Giese G, Differ C, Minkwitz S, Ruschke K, Puts R, et al. An investigation of BMP-7 mediated alterations to BMP signalling components in human tenocyte-like cells. Sci Rep. 2016;6:29703 pubmed publisher
Varadaraj A, Patel P, Serrao A, Bandyopadhay T, Lee N, Jazaeri A, et al. Epigenetic Regulation of GDF2 Suppresses Anoikis in Ovarian and Breast Epithelia. Neoplasia. 2015;17:826-38 pubmed publisher
Cao H, Jheon A, Li X, Sun Z, Wang J, Florez S, et al. The Pitx2:miR-200c/141:noggin pathway regulates Bmp signaling and ameloblast differentiation. Development. 2013;140:3348-59 pubmed publisher
product information
SKU :
6944P
Product-Name :
Smad1 (D59D7) XP® Rabbit mAb
Size :
40 ul
Price-(USD) :
135 USD
Species-x-Reactivity :
H, M, Mk, (X, B)
Applications :
Chromatin Immunoprecipitation
Product-Category :
Developmental Biology
Shipping-Temp :
AMBIENT
Storage-Temp :
-20°C
Product-Type :
Monoclonal Antibody
MW :
60
Host :
Rabbit
Target :
Smad1
Primary-Protein :
Smad1
Alt-Names :
BSP-1,BSP1,JV4-1,JV41,MAD homolog 1,MAD, mothers against decapentaplegic homolog 1,MADH1,MADR1,Mad-related protein 1,Mothers against DPP homolog 1,Mothers against decapentaplegic homolog 1,SMAD 1,SMAD family member 1,SMAD, mothers against DPP homolog 1,SMAD1,TGF-beta signaling protein 1,Transforming growth factor-beta-signaling protein 1,hSMAD1,transforming growth factor-beta signaling protein 1
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
https://www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.