product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
SirT3 (D22A3) Rabbit mAb
catalog :
5490
clonality :
monoclonal
host :
rabbit
conjugate :
nonconjugated
clone name :
D22A3
reactivity :
human, mouse, rat
application :
western blot, immunocytochemistry, immunoprecipitation, western blot knockout validation
citations: 20
Published Application/Species/Sample/DilutionReference
  • western blot knockout validation; human; loading ...; fig 1a
Peterson B, Campbell J, Ilkayeva O, Grimsrud P, Hirschey M, Newgard C. Remodeling of the Acetylproteome by SIRT3 Manipulation Fails to Affect Insulin Secretion or ? Cell Metabolism in the Absence of Overnutrition. Cell Rep. 2018;24:209-223.e6 pubmed publisher
  • western blot knockout validation; mouse; loading ...; fig 6b
He X, Zeng H, Chen S, Roman R, Aschner J, Didion S, et al. Endothelial specific SIRT3 deletion impairs glycolysis and angiogenesis and causes diastolic dysfunction. J Mol Cell Cardiol. 2017;112:104-113 pubmed publisher
  • western blot knockout validation; mouse; loading ...; fig 1a
  • immunoprecipitation; mouse; loading ...; fig 5e
  • western blot; rat; fig 3d
Li J, Chen T, Xiao M, Li N, Wang S, Su H, et al. Mouse Sirt3 promotes autophagy in AngII-induced myocardial hypertrophy through the deacetylation of FoxO1. Oncotarget. 2016;7:86648-86659 pubmed publisher
  • western blot knockout validation; mouse; fig 1
Zeng H, Vaka V, He X, Booz G, Chen J. High-fat diet induces cardiac remodelling and dysfunction: assessment of the role played by SIRT3 loss. J Cell Mol Med. 2015;19:1847-56 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 1e
Lin J, Lin J, Chen H, Chen T, Apte R. Combined SIRT3 and SIRT5 deletion is associated with inner retinal dysfunction in a mouse model of type 1 diabetes. Sci Rep. 2019;9:3799 pubmed publisher
  • western blot; human; fig s5f
Das A, Huang G, Bonkowski M, Longchamp A, Li C, Schultz M, et al. Impairment of an Endothelial NAD+-H2S Signaling Network Is a Reversible Cause of Vascular Aging. Cell. 2018;173:74-89.e20 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig s3s5
Sarikhani M, Mishra S, Maity S, Kotyada C, Wolfgeher D, Gupta M, et al. SIRT2 deacetylase regulates the activity of GSK3 isoforms independent of inhibitory phosphorylation. elife. 2018;7: pubmed publisher
  • western blot; mouse; loading ...; fig 5a
Hou Y, Lautrup S, Cordonnier S, Wang Y, Croteau D, Zavala E, et al. NAD+ supplementation normalizes key Alzheimer's features and DNA damage responses in a new AD mouse model with introduced DNA repair deficiency. Proc Natl Acad Sci U S A. 2018;115:E1876-E1885 pubmed publisher
  • western blot; mouse; 1:1000; fig 5a
Patil M, Sharma B, Elattar S, Chang J, Kapil S, Yuan J, et al. Id1 Promotes Obesity by Suppressing Brown Adipose Thermogenesis and White Adipose Browning. Diabetes. 2017;66:1611-1625 pubmed publisher
  • western blot; mouse; loading ...; fig 6
Gudiksen A, Bertholdt L, Vingborg M, Hansen H, Ringholm S, Pilegaard H. Muscle interleukin-6 and fasting-induced PDH regulation in mouse skeletal muscle. Am J Physiol Endocrinol Metab. 2017;312:E204-E214 pubmed publisher
  • western blot; mouse; loading ...; fig 4a
Liu Y, Wang T, Zhang R, Fu W, Wang X, Wang F, et al. Calorie restriction protects against experimental abdominal aortic aneurysms in mice. J Exp Med. 2016;213:2473-2488 pubmed
  • western blot; mouse; fig 4
Gurt I, Artsi H, Cohen Kfir E, Hamdani G, Ben Shalom G, Feinstein B, et al. The Sirt1 Activators SRT2183 and SRT3025 Inhibit RANKL-Induced Osteoclastogenesis in Bone Marrow-Derived Macrophages and Down-Regulate Sirt3 in Sirt1 Null Cells. PLoS ONE. 2015;10:e0134391 pubmed publisher
  • western blot; mouse
Brandauer J, Andersen M, Kellezi H, Risis S, Frøsig C, Vienberg S, et al. AMP-activated protein kinase controls exercise training- and AICAR-induced increases in SIRT3 and MnSOD. Front Physiol. 2015;6:85 pubmed publisher
  • western blot; human; 1:1000
Kim T, Kim H, Kang Y, Yoon S, Lee J, Choi W, et al. Psammaplin A induces Sirtuin 1-dependent autophagic cell death in doxorubicin-resistant MCF-7/adr human breast cancer cells and xenografts. Biochim Biophys Acta. 2015;1850:401-10 pubmed publisher
  • western blot; mouse
Le T, Urasaki Y, Pizzorno G. Uridine prevents fenofibrate-induced fatty liver. PLoS ONE. 2014;9:e87179 pubmed publisher
  • western blot; human
Kuo H, Deluca T, Miller W, Mrksich M. Profiling deacetylase activities in cell lysates with peptide arrays and SAMDI mass spectrometry. Anal Chem. 2013;85:10635-10642 pubmed publisher
  • immunocytochemistry; human; 1:50
  • western blot; human
Zhang B, Cui S, Bai X, Zhuo L, Sun X, Hong Q, et al. SIRT3 overexpression antagonizes high glucose accelerated cellular senescence in human diploid fibroblasts via the SIRT3-FOXO1 signaling pathway. Age (Dordr). 2013;35:2237-53 pubmed publisher
Chen M, Zhu X, Ran L, Lang H, Yi L, Mi M. Trimethylamine-N-Oxide Induces Vascular Inflammation by Activating the NLRP3 Inflammasome Through the SIRT3-SOD2-mtROS Signaling Pathway. J Am Heart Assoc. 2017;6: pubmed publisher
Ruiz Andrés O, Sanchez Nino M, Cannata Ortiz P, Ruiz Ortega M, Egido J, Ortiz A, et al. Histone lysine crotonylation during acute kidney injury in mice. Dis Model Mech. 2016;9:633-45 pubmed publisher
Cheung K, Cole L, Xiang B, Chen K, Ma X, Myal Y, et al. Sirtuin-3 (SIRT3) Protein Attenuates Doxorubicin-induced Oxidative Stress and Improves Mitochondrial Respiration in H9c2 Cardiomyocytes. J Biol Chem. 2015;290:10981-93 pubmed publisher
product information
SKU :
5490S
Product-Name :
SirT3 (D22A3) Rabbit mAb
Size :
100 ul
Price-(USD) :
235 USD
Species-x-Reactivity :
H, M, R
Applications :
Immunoprecipitation
Product-Category :
Chromatin Regulation / Nuclear Function
Shipping-Temp :
AMBIENT
Storage-Temp :
-20°C
Product-Type :
Monoclonal Antibody
MW :
28
Host :
Rabbit
Target :
SirT3
Primary-Protein :
SIRT3
Alt-Names :
NAD-dependent deacetylase sirtuin-3, mitochondrial,SIR2-like protein 3,SIR2L3,SIRT3,hSIRT3,mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase,silent mating type information regulation 2, S.cerevisiae, homolog 3,sir2-like 3,sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae),sirtuin type 3
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
https://www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.