product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
Nanog (D73G4) XP® Rabbit mAb (ChIP Formulated)
catalog :
5232
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
D73G4
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section
citations: 32
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; human; 1:250; loading ...; fig 5d
Weltner J, Balboa D, Katayama S, Bespalov M, Krjutskov K, Jouhilahti E, et al. Human pluripotent reprogramming with CRISPR activators. Nat Commun. 2018;9:2643 pubmed publisher
  • flow cytometry; human; 1:200; loading ...; fig 1b
Zhu L, Gómez Durán A, Saretzki G, Jin S, Tilgner K, Melguizo Sanchís D, et al. The mitochondrial protein CHCHD2 primes the differentiation potential of human induced pluripotent stem cells to neuroectodermal lineages. J Cell Biol. 2016;215:187-202 pubmed
  • immunohistochemistry - paraffin section; human; 1:500; loading ...; fig 1d
Baillie R, Itinteang T, Yu H, Brasch H, Davis P, Tan S. Cancer stem cells in moderately differentiated oral tongue squamous cell carcinoma. J Clin Pathol. 2016;69:742-4 pubmed publisher
  • immunocytochemistry; human; 1:300; fig S1B
Stratigopoulos G, Burnett L, Rausch R, Gill R, Penn D, Skowronski A, et al. Hypomorphism of Fto and Rpgrip1l causes obesity in mice. J Clin Invest. 2016;126:1897-910 pubmed publisher
  • chromatin immunoprecipitation; human; 1:100; fig 5b
  • western blot; human; loading ...; fig 5c
Wang X, Jung Y, Jun S, Lee S, Wang W, Schneider A, et al. PAF-Wnt signaling-induced cell plasticity is required for maintenance of breast cancer cell stemness. Nat Commun. 2016;7:10633 pubmed publisher
  • immunocytochemistry; human; fig s1
Kyttälä A, Moraghebi R, Valensisi C, Kettunen J, Andrus C, Pasumarthy K, et al. Genetic Variability Overrides the Impact of Parental Cell Type and Determines iPSC Differentiation Potential. Stem Cell Reports. 2016;6:200-12 pubmed publisher
  • immunocytochemistry; human; 1:500; loading ...; fig 1e
Balboa D, Weltner J, Eurola S, Trokovic R, Wartiovaara K, Otonkoski T. Conditionally Stabilized dCas9 Activator for Controlling Gene Expression in Human Cell Reprogramming and Differentiation. Stem Cell Reports. 2015;5:448-59 pubmed publisher
  • western blot; human; fig 1
Kawamura N, Nimura K, Nagano H, Yamaguchi S, Nonomura N, Kaneda Y. CRISPR/Cas9-mediated gene knockout of NANOG and NANOGP8 decreases the malignant potential of prostate cancer cells. Oncotarget. 2015;6:22361-74 pubmed
  • western blot; human; fig 5
Palla A, Piazzolla D, Alcazar N, Cañamero M, Graña O, Gómez López G, et al. The pluripotency factor NANOG promotes the formation of squamous cell carcinomas. Sci Rep. 2015;5:10205 pubmed publisher
  • immunocytochemistry; human; 1:200
Shimamoto A, Kagawa H, Zensho K, Sera Y, Kazuki Y, Osaki M, et al. Reprogramming suppresses premature senescence phenotypes of Werner syndrome cells and maintains chromosomal stability over long-term culture. PLoS ONE. 2014;9:e112900 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:50; fig 7
  • western blot; mouse; 1:1000; fig 7
Piazzolla D, Palla A, Pantoja C, Canamero M, de Castro I, Ortega S, et al. Lineage-restricted function of the pluripotency factor NANOG in stratified epithelia. Nat Commun. 2014;5:4226 pubmed publisher
  • immunohistochemistry; human; 1:300
  • western blot; human; 1:1000
Chen W, Ho C, Chang Y, Chen H, Lin C, Ling T, et al. Cancer-associated fibroblasts regulate the plasticity of lung cancer stemness via paracrine signalling. Nat Commun. 2014;5:3472 pubmed publisher
Aban C, Lombardi A, Neiman G, Biani M, La Greca A, Waisman A, et al. Downregulation of E-cadherin in pluripotent stem cells triggers partial EMT. Sci Rep. 2021;11:2048 pubmed publisher
Grajcarek J, Monlong J, Nishinaka Arai Y, Nakamura M, Nagai M, Matsuo S, et al. Genome-wide microhomologies enable precise template-free editing of biologically relevant deletion mutations. Nat Commun. 2019;10:4856 pubmed publisher
Kozono S, Lin Y, Seo H, Pinch B, Lian X, Qiu C, et al. Arsenic targets Pin1 and cooperates with retinoic acid to inhibit cancer-driving pathways and tumor-initiating cells. Nat Commun. 2018;9:3069 pubmed publisher
Ooki A, Dinalankara W, Marchionni L, Tsay J, Goparaju C, Maleki Z, et al. Epigenetically regulated PAX6 drives cancer cells toward a stem-like state via GLI-SOX2 signaling axis in lung adenocarcinoma. Oncogene. 2018;37:5967-5981 pubmed publisher
Jonikas M, Madill M, Mathy A, Zekoll T, Zois C, Wigfield S, et al. Stem cell modeling of mitochondrial parkinsonism reveals key functions of OPA1. Ann Neurol. 2018;83:915-925 pubmed publisher
Bratkovic T, Modic M, Camargo Ortega G, Drukker M, Rogelj B. Neuronal differentiation induces SNORD115 expression and is accompanied by post-transcriptional changes of serotonin receptor 2c mRNA. Sci Rep. 2018;8:5101 pubmed publisher
Ooki A, Begum A, Marchionni L, Vandenbussche C, Mao S, Kates M, et al. Arsenic promotes the COX2/PGE2-SOX2 axis to increase the malignant stemness properties of urothelial cells. Int J Cancer. 2018;143:113-126 pubmed publisher
Buonocore F, Kühnen P, Suntharalingham J, Del Valle I, Digweed M, Stachelscheid H, et al. Somatic mutations and progressive monosomy modify SAMD9-related phenotypes in humans. J Clin Invest. 2017;127:1700-1713 pubmed publisher
Lillo M, Nichols C, Perry C, Runke S, Krutilina R, Seagroves T, et al. Methylparaben stimulates tumor initiating cells in ER+ breast cancer models. J Appl Toxicol. 2017;37:417-425 pubmed publisher
Cigognini D, Gaspar D, Kumar P, Satyam A, Alagesan S, Sanz Nogués C, et al. Macromolecular crowding meets oxygen tension in human mesenchymal stem cell culture - A step closer to physiologically relevant in vitro organogenesis. Sci Rep. 2016;6:30746 pubmed publisher
Gayle S, Pan Y, Landrette S, Xu T. piggyBac insertional mutagenesis screen identifies a role for nuclear RHOA in human ES cell differentiation. Stem Cell Reports. 2015;4:926-38 pubmed publisher
Miyatake Y, Sheehy N, Ikeshita S, Hall W, Kasahara M. Anchorage-dependent multicellular aggregate formation induces CD44 high cancer stem cell-like ATL cells in an NF-κB- and vimentin-dependent manner. Cancer Lett. 2015;357:355-63 pubmed publisher
Kregel S, Szmulewitz R, Vander Griend D. The pluripotency factor Nanog is directly upregulated by the androgen receptor in prostate cancer cells. Prostate. 2014;74:1530-43 pubmed publisher
Miettinen M, Wang Z, McCue P, Sarlomo Rikala M, Rys J, Biernat W, et al. SALL4 expression in germ cell and non-germ cell tumors: a systematic immunohistochemical study of 3215 cases. Am J Surg Pathol. 2014;38:410-20 pubmed publisher
Vuoristo S, Toivonen S, Weltner J, Mikkola M, Ustinov J, Trokovic R, et al. A novel feeder-free culture system for human pluripotent stem cell culture and induced pluripotent stem cell derivation. PLoS ONE. 2013;8:e76205 pubmed publisher
Rybak A, Tang D. SOX2 plays a critical role in EGFR-mediated self-renewal of human prostate cancer stem-like cells. Cell Signal. 2013;25:2734-42 pubmed publisher
Romorini L, Riva D, Bluguermann C, Videla Richardson G, Scassa M, Sevlever G, et al. Effect of antibiotics against Mycoplasma sp. on human embryonic stem cells undifferentiated status, pluripotency, cell viability and growth. PLoS ONE. 2013;8:e70267 pubmed publisher
Kregel S, Kiriluk K, Rosen A, Cai Y, Reyes E, Otto K, et al. Sox2 is an androgen receptor-repressed gene that promotes castration-resistant prostate cancer. PLoS ONE. 2013;8:e53701 pubmed publisher
Chae H, Lee M, Broxmeyer H. 5-Aminoimidazole-4-carboxyamide ribonucleoside induces G(1)/S arrest and Nanog downregulation via p53 and enhances erythroid differentiation. Stem Cells. 2012;30:140-9 pubmed publisher
Ledur P, Villodre E, Paulus R, Cruz L, Flores D, Lenz G. Extracellular ATP reduces tumor sphere growth and cancer stem cell population in glioblastoma cells. Purinergic Signal. 2012;8:39-48 pubmed publisher
product information
SKU :
5232S
Product-Name :
Nanog (D73G4) XP® Rabbit mAb (ChIP Formulated)
Size :
100 ul
Price-(USD) :
260 USD
Species-x-Reactivity :
H
Applications :
Chromatin Immunoprecipitation
Product-Category :
Developmental Biology
Shipping-Temp :
AMBIENT
Storage-Temp :
-20°C
Product-Type :
Monoclonal Antibody
MW :
42
Host :
Rabbit
Target :
Nanog
Primary-Protein :
NANOG
Alt-Names :
Homeobox protein NANOG,Homeobox transcription factor Nanog,NANOG,Nanog homeobox,hNanog,homeobox transcription factor Nanog-delta 48
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
https://www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.