product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
Bax (D2E11) Rabbit mAb
catalog :
5023
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
D2E11
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section, western blot knockout validation
citations: 67
Published Application/Species/Sample/DilutionReference
  • western blot knockout validation; human; loading ...; fig 3b
Bahr J, Robey R, Luchenko V, Basseville A, Chakraborty A, Kozlowski H, et al. Blocking downstream signaling pathways in the context of HDAC inhibition promotes apoptosis preferentially in cells harboring mutant Ras. Oncotarget. 2016;7:69804-69815 pubmed publisher
  • immunoprecipitation; human; loading ...; fig 6d
  • western blot; human; loading ...; fig 6a
Yan D, Li X, Yang Q, Huang Q, Yao L, Zhang P, et al. Regulation of Bax-dependent apoptosis by mitochondrial deubiquitinase USP30. Cell Death Discov. 2021;7:211 pubmed publisher
  • western blot; rat; 1:1000; loading ...; fig 2c
Meng L, Zhang Y, Li D, Shang X, Hao X, Chen X, et al. TIMP3 attenuates cerebral ischemia/reperfusion-induced apoptosis and oxidative stress in neurocytes by regulating the AKT pathway. Exp Ther Med. 2021;22:973 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 7d
Li Q, Liu M, Sun Y, Jin T, Zhu P, Wan X, et al. SLC6A8-mediated intracellular creatine accumulation enhances hypoxic breast cancer cell survival via ameliorating oxidative stress. J Exp Clin Cancer Res. 2021;40:168 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 4d, 4f
Hu J, Wang J, Li C, Shang Y. Fructose-1,6-bisphosphatase aggravates oxidative stress-induced apoptosis in asthma by suppressing the Nrf2 pathway. J Cell Mol Med. 2021;25:5001-5014 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 4a
  • western blot; mouse; 1:1000; loading ...; fig 7c
Zhou H, Li G, Wang Y, Jiang R, Li Y, Wang H, et al. Microbial Metabolite Sodium Butyrate Attenuates Cartilage Degradation by Restoring Impaired Autophagy and Autophagic Flux in Osteoarthritis Development. Front Pharmacol. 2021;12:659597 pubmed publisher
  • western blot; human; 1:100; loading ...; fig 5b
Chen W, Wu C, Chen Y, Guo Y, Qiu L, Liu Z, et al. Downregulation of ceramide synthase 1 promotes oral cancer through endoplasmic reticulum stress. Int J Oral Sci. 2021;13:10 pubmed publisher
  • western blot; mouse; loading ...; fig 5c
Sun Q, Chen J, Xu L, Kang J, Wu X, Ren Y, et al. MUTYH Deficiency Is Associated with Attenuated Pulmonary Fibrosis in a Bleomycin-Induced Model. Oxid Med Cell Longev. 2020;2020:4828256 pubmed publisher
  • western blot; human; fig 2a
Gu J, Zhang Y, Wang X, Xiang J, Deng S, Wu D, et al. Matrine inhibits the growth of natural killer/T-cell lymphoma cells by modulating CaMKIIγ-c-Myc signaling pathway. BMC Complement Med Ther. 2020;20:214 pubmed publisher
  • western blot; human; loading ...; fig 4f
Yin S, Song M, Zhao R, Liu X, Kang W, Lee J, et al. Xanthohumol Inhibits the Growth of Keratin 18-Overexpressed Esophageal Squamous Cell Carcinoma in vitro and in vivo. Front Cell Dev Biol. 2020;8:366 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 4c
Shen S, Dean D, Yu Z, Hornicek F, Kan Q, Duan Z. Aberrant CDK9 expression within chordoma tissues and the therapeutic potential of a selective CDK9 inhibitor LDC000067. J Cancer. 2020;11:132-141 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 2f
Tian M, Gong W, Guo J. Long non-coding RNA SNHG1 indicates poor prognosis and facilitates disease progression in acute myeloid leukemia. Biol Open. 2019;8: pubmed publisher
  • western blot; human; loading ...; fig 2c
Hou N, He X, Yang Y, Fu J, Zhang W, Guo Z, et al. TRPV1 Induced Apoptosis of Colorectal Cancer Cells by Activating Calcineurin-NFAT2-p53 Signaling Pathway. Biomed Res Int. 2019;2019:6712536 pubmed publisher
  • western blot; human; loading ...; fig 4f
Pan C, Jin L, Wang X, Li Y, Chun J, Boese A, et al. Inositol-triphosphate 3-kinase B confers cisplatin resistance by regulating NOX4-dependent redox balance. J Clin Invest. 2019;129:2431-2445 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 7d
Liu X, Chen H, Hou Y, Ma X, Ye M, Huang R, et al. Adaptive EGF expression sensitizes pancreatic cancer cells to ionizing radiation through activation of the cyclin D1/P53/PARP pathway. Int J Oncol. 2019;54:1466-1480 pubmed publisher
  • western blot; human; loading ...; fig 2g
Lee E, Ouzounova M, Piranlioglu R, Ma M, Guzel M, Marasco D, et al. The pleiotropic effects of TNFα in breast cancer subtypes is regulated by TNFAIP3/A20. Oncogene. 2019;38:469-482 pubmed publisher
  • western blot; human; 1:1000; fig s3a
Zhang S, Zhang M, Jing Y, Yin X, Ma P, Zhang Z, et al. Deubiquitinase USP13 dictates MCL1 stability and sensitivity to BH3 mimetic inhibitors. Nat Commun. 2018;9:215 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig s8c
Mai W, Gosa L, Daniëls V, Ta L, Tsang J, Higgins B, et al. Cytoplasmic p53 couples oncogene-driven glucose metabolism to apoptosis and is a therapeutic target in glioblastoma. Nat Med. 2017;23:1342-1351 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 2e
Yang X, Zhao Q, Yin H, Lei X, Gan R. MiR-33b-5p sensitizes gastric cancer cells to chemotherapy drugs via inhibiting HMGA2 expression. J Drug Target. 2017;25:653-660 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 4
Hou J, Xue J, Lee M, Sung C. Ginsenoside Rd as a potential neuroprotective agent prevents trimethyltin injury. Biomed Rep. 2017;6:435-440 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 8b
Yokoyama T, Kohn E, Brill E, Lee J. Apoptosis is augmented in high-grade serous ovarian cancer by the combined inhibition of Bcl-2/Bcl-xL and PARP. Int J Oncol. 2017;: pubmed publisher
  • western blot; human; 1:1000; loading ...; fig s2e
Villar V, Nguyen T, Delcroix V, Terés S, Bouchecareilh M, Salin B, et al. mTORC1 inhibition in cancer cells protects from glutaminolysis-mediated apoptosis during nutrient limitation. Nat Commun. 2017;8:14124 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 3c
Liu G, Xiang T, Wu Q, Wang W. Curcumin suppresses the proliferation of gastric cancer cells by downregulating H19. Oncol Lett. 2016;12:5156-5162 pubmed publisher
  • western blot; human; loading ...; fig 2d
Cao H, Yu S, Chen D, Jing C, Wang Z, Ma R, et al. Liver X receptor agonist T0901317 reverses resistance of A549 human lung cancer cells to EGFR-TKI treatment. FEBS Open Bio. 2017;7:35-43 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 5a
He X, Liu Z, Xia Y, Xu J, Lv G, Wang L, et al. HOXB7 overexpression promotes cell proliferation and correlates with poor prognosis in gastric cancer patients by inducing expression of both AKT and MARKs. Oncotarget. 2017;8:1247-1261 pubmed publisher
  • western blot; human; loading ...; fig 4b
Wang C, Zhang F, Cao Y, Zhang M, Wang A, Xu M, et al. Etoposide Induces Apoptosis in Activated Human Hepatic Stellate Cells via ER Stress. Sci Rep. 2016;6:34330 pubmed publisher
  • western blot; human; 1:2000; loading ...; fig 6c
Cao R, Meng Z, Liu T, Wang G, Qian G, Cao T, et al. Decreased TRPM7 inhibits activities and induces apoptosis of bladder cancer cells via ERK1/2 pathway. Oncotarget. 2016;7:72941-72960 pubmed publisher
  • western blot; human; loading ...; fig 5d
Klingbeil O, Lesche R, Gelato K, Haendler B, Lejeune P. Inhibition of BET bromodomain-dependent XIAP and FLIP expression sensitizes KRAS-mutated NSCLC to pro-apoptotic agents. Cell Death Dis. 2016;7:e2365 pubmed publisher
  • western blot; human; loading ...; fig 5b
Cheng S, Jiang X, Ding C, Du C, Owusu Ansah K, Weng X, et al. Expression and Critical Role of Interleukin Enhancer Binding Factor 2 in Hepatocellular Carcinoma. Int J Mol Sci. 2016;17: pubmed publisher
  • western blot; human; loading ...; fig 4b
Liu H, Li W, Yu X, Gao F, Duan Z, Ma X, et al. EZH2-mediated Puma gene repression regulates non-small cell lung cancer cell proliferation and cisplatin-induced apoptosis. Oncotarget. 2016;7:56338-56354 pubmed publisher
  • western blot; human; fig 5
Ma T, Fan B, Zhang C, Zhao H, Han C, Gao C, et al. Metabonomics applied in exploring the antitumour mechanism of physapubenolide on hepatocellular carcinoma cells by targeting glycolysis through the Akt-p53 pathway. Sci Rep. 2016;6:29926 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 4b
Gao Z, Liu Z, Bi M, Zhang J, Han Z, Han X, et al. Metformin induces apoptosis via a mitochondria-mediated pathway in human breast cancer cells in vitro. Exp Ther Med. 2016;11:1700-1706 pubmed
  • western blot; human; 1:1000; fig 1
Yu L, Wu W, Gu C, Zhong D, Zhao X, Kong Y, et al. Obatoclax impairs lysosomal function to block autophagy in cisplatin-sensitive and -resistant esophageal cancer cells. Oncotarget. 2016;7:14693-707 pubmed publisher
  • western blot; human; fig s6
Kline C, van den Heuvel A, Allen J, Prabhu V, Dicker D, El Deiry W. ONC201 kills solid tumor cells by triggering an integrated stress response dependent on ATF4 activation by specific eIF2α kinases. Sci Signal. 2016;9:ra18 pubmed publisher
  • western blot; human; 1:2000; fig 3
Zhang Y, Zou C, Yang S, Fu J. P120 catenin attenuates the angiotensin II-induced apoptosis of human umbilical vein endothelial cells by suppressing the mitochondrial pathway. Int J Mol Med. 2016;37:623-30 pubmed publisher
  • western blot; human; fig 5
Wang F, Feng Y, Li P, Wang K, Feng L, Liu Y, et al. RASSF10 is an epigenetically inactivated tumor suppressor and independent prognostic factor in hepatocellular carcinoma. Oncotarget. 2016;7:4279-97 pubmed publisher
  • western blot; human; loading ...; fig 4c, 5d
Huang C, Lee C, Lin H, Chen M, Lin C, Chang J. Autophagy-Regulated ROS from Xanthine Oxidase Acts as an Early Effector for Triggering Late Mitochondria-Dependent Apoptosis in Cathepsin S-Targeted Tumor Cells. PLoS ONE. 2015;10:e0128045 pubmed publisher
  • western blot; human; 1:1000; fig 3c
Wei Z, Yu D, Bi Y, Cao Y. A disintegrin and metalloprotease 17 promotes microglial cell survival via epidermal growth factor receptor signalling following spinal cord injury. Mol Med Rep. 2015;12:63-70 pubmed publisher
  • western blot; human; fig 2
Vogel C, Smit M, Maddalo G, Possik P, Sparidans R, van der Burg S, et al. Cooperative induction of apoptosis in NRAS mutant melanoma by inhibition of MEK and ROCK. Pigment Cell Melanoma Res. 2015;28:307-17 pubmed publisher
  • western blot; human; fig 6
Zhong T, Xu F, Xu J, Liu L, Chen Y. Aldo-keto reductase 1C3 (AKR1C3) is associated with the doxorubicin resistance in human breast cancer via PTEN loss. Biomed Pharmacother. 2015;69:317-25 pubmed publisher
  • western blot; human; fig 6b
Wang S, Geng Z, Shi N, Li X, Wang Z. Dose-dependent effects of selenite (Se(4+)) on arsenite (As(3+))-induced apoptosis and differentiation in acute promyelocytic leukemia cells. Cell Death Dis. 2015;6:e1596 pubmed publisher
  • western blot; human; fig s3
Senturk S, Yao Z, Camiolo M, Stiles B, Rathod T, Walsh A, et al. p53? is a transcriptionally inactive p53 isoform able to reprogram cells toward a metastatic-like state. Proc Natl Acad Sci U S A. 2014;111:E3287-96 pubmed publisher
  • western blot; human
Havel J, Li Z, Cheng D, Peng J, Fu H. Nuclear PRAS40 couples the Akt/mTORC1 signaling axis to the RPL11-HDM2-p53 nucleolar stress response pathway. Oncogene. 2015;34:1487-98 pubmed publisher
  • western blot; human; 1:1000
Sun S, Han Y, Liu J, Fang Y, Tian Y, Zhou J, et al. Trichostatin A targets the mitochondrial respiratory chain, increasing mitochondrial reactive oxygen species production to trigger apoptosis in human breast cancer cells. PLoS ONE. 2014;9:e91610 pubmed publisher
  • western blot; human
Pavet V, Shlyakhtina Y, He T, Ceschin D, Kohonen P, Perala M, et al. Plasminogen activator urokinase expression reveals TRAIL responsiveness and supports fractional survival of cancer cells. Cell Death Dis. 2014;5:e1043 pubmed publisher
  • western blot; human
Sanders Y, Liu H, Zhang X, Hecker L, Bernard K, Desai L, et al. Histone modifications in senescence-associated resistance to apoptosis by oxidative stress. Redox Biol. 2013;1:8-16 pubmed publisher
  • western blot; human
Song J, Peng X, Ji M, Ai M, Zhang J, Dong W. Hugl-1 induces apoptosis in esophageal carcinoma cells both in vitro and in vivo. World J Gastroenterol. 2013;19:4127-36 pubmed publisher
  • western blot; human; 1:1000
Mo Q, Chen P, Jin X, Chen Q, Tang L, Wang B, et al. Inhibition of Hec1 expression enhances the sensitivity of human ovarian cancer cells to paclitaxel. Acta Pharmacol Sin. 2013;34:541-8 pubmed publisher
  • western blot; human
Li L, Yang G, Ren C, Tanimoto R, Hirayama T, Wang J, et al. Glioma pathogenesis-related protein 1 induces prostate cancer cell death through Hsc70-mediated suppression of AURKA and TPX2. Mol Oncol. 2013;7:484-96 pubmed publisher
  • western blot; human
Nakamura K, Aizawa K, Nakabayashi K, Kato N, Yamauchi J, Hata K, et al. DNA methyltransferase inhibitor zebularine inhibits human hepatic carcinoma cells proliferation and induces apoptosis. PLoS ONE. 2013;8:e54036 pubmed publisher
Hii L, Lim S, Leong C, Chin S, Tan N, Lai K, et al. The synergism of Clinacanthus nutans Lindau extracts with gemcitabine: downregulation of anti-apoptotic markers in squamous pancreatic ductal adenocarcinoma. BMC Complement Altern Med. 2019;19:257 pubmed publisher
Deryabin P, Griukova A, Shatrova A, Petukhov A, Nikolsky N, Borodkina A. Optimization of lentiviral transduction parameters and its application for CRISPR-based secretome modification of human endometrial mesenchymal stem cells. Cell Cycle. 2019;18:742-758 pubmed publisher
Poon A, Inkol J, Luu A, Mutsaers A. Effects of the potassium-sparing diuretic amiloride on chemotherapy response in canine osteosarcoma cells. J Vet Intern Med. 2019;33:800-811 pubmed publisher
Ward A, Mir H, Kapur N, Gales D, Carriere P, Singh S. Quercetin inhibits prostate cancer by attenuating cell survival and inhibiting anti-apoptotic pathways. World J Surg Oncol. 2018;16:108 pubmed publisher
Li Y, Liang Y, Sang Y, Song X, Zhang H, Liu Y, et al. MiR-770 suppresses the chemo-resistance and metastasis of triple negative breast cancer via direct targeting of STMN1. Cell Death Dis. 2018;9:14 pubmed publisher
Turczyk L, Kitowska K, Mieszkowska M, Mieczkowski K, Czaplinska D, Piasecka D, et al. FGFR2-Driven Signaling Counteracts Tamoxifen Effect on ER?-Positive Breast Cancer Cells. Neoplasia. 2017;19:791-804 pubmed publisher
Hu Y, Qiu Y, Yagüe E, Ji W, Liu J, Zhang J. miRNA-205 targets VEGFA and FGF2 and regulates resistance to chemotherapeutics in breast cancer. Cell Death Dis. 2016;7:e2291 pubmed publisher
Li L, Chen X, Liu C, Lee L, Man C, Cheng S. Phytoestrogen Bakuchiol Exhibits In Vitro and In Vivo Anti-breast Cancer Effects by Inducing S Phase Arrest and Apoptosis. Front Pharmacol. 2016;7:128 pubmed publisher
Li X, Lao Y, Zhang H, Wang X, Tan H, Lin Z, et al. The natural compound Guttiferone F sensitizes prostate cancer to starvation induced apoptosis via calcium and JNK elevation. BMC Cancer. 2015;15:254 pubmed publisher
Wei W, Li L, Wang X, Yan L, Cao W, Zhan Z, et al. Overexpression of caudal type homeobox transcription factor 2 inhibits the growth of the MGC-803 human gastric cancer cell line in vivo. Mol Med Rep. 2015;12:905-12 pubmed publisher
Shu Q, Li W, Li H, Sun G. Vasostatin inhibits VEGF-induced endothelial cell proliferation, tube formation and induces cell apoptosis under oxygen deprivation. Int J Mol Sci. 2014;15:6019-30 pubmed publisher
Peng Z, Liu M, Fang Z, Chen L, Wu J, Zhang Q. In vitro antiproliferative effect of a water-soluble Laminaria japonica polysaccharide on human melanoma cell line A375. Food Chem Toxicol. 2013;58:56-60 pubmed publisher
Endo A, Koizumi H, Takahashi M, Tamura T, Tatsunami S, Watanabe Y, et al. A significant imbalance in mitosis versus apoptosis accelerates the growth rate of sessile serrated adenoma/polyps. Virchows Arch. 2013;462:131-9 pubmed publisher
Santag S, Jager W, Karsten C, Kati S, Pietrek M, Steinemann D, et al. Recruitment of the tumour suppressor protein p73 by Kaposi's Sarcoma Herpesvirus latent nuclear antigen contributes to the survival of primary effusion lymphoma cells. Oncogene. 2013;32:3676-85 pubmed publisher
Ifeadi V, Garnett Benson C. Sub-lethal irradiation of human colorectal tumor cells imparts enhanced and sustained susceptibility to multiple death receptor signaling pathways. PLoS ONE. 2012;7:e31762 pubmed publisher
Weisberg E, Ray A, Nelson E, Adamia S, Barrett R, Sattler M, et al. Reversible resistance induced by FLT3 inhibition: a novel resistance mechanism in mutant FLT3-expressing cells. PLoS ONE. 2011;6:e25351 pubmed publisher
Kim K, Srikanth S, Yee M, Mock D, Lawson G, Gwack Y. ORAI1 deficiency impairs activated T cell death and enhances T cell survival. J Immunol. 2011;187:3620-30 pubmed publisher
product information
SKU :
5023S
Product-Name :
Bax (D2E11) Rabbit mAb
Size :
100 ul
Price-(USD) :
235 USD
Species-x-Reactivity :
H
Applications :
Immunohistochemistry (Paraffin)
Product-Category :
Apoptosis
Shipping-Temp :
COLD
Storage-Temp :
-20°C
Product-Type :
Monoclonal Antibody
MW :
20
Host :
Rabbit
Target :
Bax
Primary-Protein :
Bax
Alt-Names :
Apoptosis regulator BAX,BAX,BCL2-associated X protein,BCL2L4,Bcl-2-like protein 4,Bcl2-L-4
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
https://www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.