product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (Biotinylated)
catalog :
5012
clonality :
monoclonal
host :
rabbit
conjugate :
biotin
antigen modification :
phosphorylated
clone name :
D9E
reactivity :
human, mouse, rat, cow
application :
western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section
more info or order :
Cell Signaling Technology product webpage
citations: 14
Published Application/Species/Sample/DilutionReference
  • western blot; human; fig 6
Ma T, Fan B, Zhang C, Zhao H, Han C, Gao C, et al. Metabonomics applied in exploring the antitumour mechanism of physapubenolide on hepatocellular carcinoma cells by targeting glycolysis through the Akt-p53 pathway. Sci Rep. 2016;6:29926 pubmed publisher
  • western blot; human; loading ...; fig 1a
Xu K, Wang L, Feng W, Feng Y, Shu H. Phosphatidylinositol-3 kinase-dependent translational regulation of Id1 involves the PPM1G phosphatase. Oncogene. 2016;35:5807-5816 pubmed publisher
  • flow cytometry; mouse; fig 5
Apostolidis S, Rodríguez Rodríguez N, Suárez Fueyo A, Dioufa N, Ozcan E, Crispín J, et al. Phosphatase PP2A is requisite for the function of regulatory T cells. Nat Immunol. 2016;17:556-64 pubmed publisher
  • western blot; mouse; fig 3
Kral J, Kuttke M, Schrottmaier W, Birnecker B, Warszawska J, Wernig C, et al. Sustained PI3K Activation exacerbates BLM-induced Lung Fibrosis via activation of pro-inflammatory and pro-fibrotic pathways. Sci Rep. 2016;6:23034 pubmed publisher
  • western blot; human; loading ...; fig s8
Chen S, FORRESTER W, Lahav G. Schedule-dependent interaction between anticancer treatments. Science. 2016;351:1204-8 pubmed publisher
  • western blot; human; 1:1000; fig 4
Egawa H, Jingushi K, Hirono T, Ueda Y, Kitae K, Nakata W, et al. The miR-130 family promotes cell migration and invasion in bladder cancer through FAK and Akt phosphorylation by regulating PTEN. Sci Rep. 2016;6:20574 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:500; fig 6
Benedykcinska A, Ferreira A, Lau J, Broni J, Richard Loendt A, Henriquez N, et al. Generation of brain tumours in mice by Cre-mediated recombination of neural progenitors in situ with the tamoxifen metabolite endoxifen. Dis Model Mech. 2016;9:211-20 pubmed publisher
  • western blot; human; fig 5
  • western blot; mouse; fig 5
Vural A, Al Khodor S, Cheung G, Shi C, Srinivasan L, McQuiston T, et al. Activator of G-Protein Signaling 3-Induced Lysosomal Biogenesis Limits Macrophage Intracellular Bacterial Infection. J Immunol. 2016;196:846-56 pubmed publisher
  • flow cytometry; human; loading ...; fig s10a
Lin H, Masaki H, Yamaguchi T, Wada T, Yachie A, Nishimura K, et al. An assessment of the effects of ectopic gp91phox expression in XCGD iPSC-derived neutrophils. Mol Ther Methods Clin Dev. 2015;2:15046 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3h
Li Y, Shen C, Zhu B, Shi F, Eisen H, Chen J. Persistent Antigen and Prolonged AKT-mTORC1 Activation Underlie Memory CD8 T Cell Impairment in the Absence of CD4 T Cells. J Immunol. 2015;195:1591-8 pubmed publisher
  • western blot; mouse; loading ...; fig 1d
  • immunohistochemistry; human; loading ...; fig 2c
Zidek L, Ackermann T, Hartleben G, Eichwald S, Kortman G, Kiehntopf M, et al. Deficiency in mTORC1-controlled C/EBPβ-mRNA translation improves metabolic health in mice. EMBO Rep. 2015;16:1022-36 pubmed publisher
  • western blot; human; fig 2
Momeny M, Saunus J, Marturana F, McCart Reed A, Black D, Sala G, et al. Heregulin-HER3-HER2 signaling promotes matrix metalloproteinase-dependent blood-brain-barrier transendothelial migration of human breast cancer cell lines. Oncotarget. 2015;6:3932-46 pubmed
  • western blot; mouse; fig 7
Shrestha S, Yang K, Guy C, Vogel P, Neale G, Chi H. Treg cells require the phosphatase PTEN to restrain TH1 and TFH cell responses. Nat Immunol. 2015;16:178-87 pubmed publisher
Horibe Y, Adachi S, Yasuda I, Yamauchi T, Kawaguchi J, Kozawa O, et al. Anticancer effect of arsenite on cell migration, cell cycle and apoptosis in human pancreatic cancer cells. Oncol Lett. 2016;12:177-182 pubmed
product information
SKU :
5012S
Product-Name :
Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (Biotinylated)
Size :
100 ul
Price-(USD) :
321 USD
Species-x-Reactivity :
H, M, R, Hm, Mk, Dm, Z, B, (C, X, Dg, Pg)
Applications :
Flow cytometry
Product-Category :
PI3K / Akt Signaling
Shipping-Temp :
AMBIENT
Storage-Temp :
-20°C
Product-Type :
Monoclonal Antibody
MW :
60
Host :
Rabbit
Target :
Akt1 (Ser473) phosphate/Akt2 (Ser474) phosphate/Akt3 (Ser472) phosphate
more info or order :
Cell Signaling Technology product webpage
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.