product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
N-WASP (30D10) Rabbit mAb
catalog :
4848
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
30D10
reactivity :
human, mouse, rat, dogs, bovine
application :
western blot, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section, western blot knockout validation, immunohistochemistry knockout validation
citations: 26
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry knockout validation; mouse; fig s2
  • western blot knockout validation; mouse; fig 1a
Schell C, Baumhakl L, Salou S, Conzelmann A, Meyer C, Helmstädter M, et al. N-wasp is required for stabilization of podocyte foot processes. J Am Soc Nephrol. 2013;24:713-21 pubmed publisher
  • immunohistochemistry knockout validation; mouse; 1:500; loading ...; fig 1a
Juin A, Spence H, Martin K, McGhee E, Neilson M, Cutiongco M, et al. N-WASP Control of LPAR1 Trafficking Establishes Response to Self-Generated LPA Gradients to Promote Pancreatic Cancer Cell Metastasis. Dev Cell. 2019;51:431-445.e7 pubmed publisher
  • immunoprecipitation; human; 1:50; loading ...; fig s5b
  • western blot; human; 1:1000; loading ...; fig s5a
Zhang Y, Zhong Z, Li M, Chen J, Lin T, Sun J, et al. The roles and prognostic significance of ABI1-TSV-11 expression in patients with left-sided colorectal cancer. Sci Rep. 2021;11:10734 pubmed publisher
  • western blot; human; 1:200; loading ...; fig 7c
Fayad R, Rojas M, Partisani M, Finetti P, Dib S, Abélanet S, et al. EFA6B regulates a stop signal for collective invasion in breast cancer. Nat Commun. 2021;12:2198 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig s2g
Schell C, Sabass B, Helmstaedter M, Geist F, Abed A, Yasuda Yamahara M, et al. ARP3 Controls the Podocyte Architecture at the Kidney Filtration Barrier. Dev Cell. 2018;47:741-757.e8 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 6a
Rivkin N, Chapnik E, Mildner A, Barshtein G, Porat Z, Kartvelishvily E, et al. Erythrocyte survival is controlled by microRNA-142. Haematologica. 2017;102:676-685 pubmed publisher
  • western blot; human; loading ...; fig s2b
Baron L, Paatero A, Morel J, Impens F, Guenin Macé L, Saint Auret S, et al. Mycolactone subverts immunity by selectively blocking the Sec61 translocon. J Exp Med. 2016;213:2885-2896 pubmed
  • immunocytochemistry; human; 1:100; fig s4
Qi L, Jafari N, Li X, Chen Z, Li L, Hytönen V, et al. Talin2-mediated traction force drives matrix degradation and cell invasion. J Cell Sci. 2016;129:3661-3674 pubmed
  • immunocytochemistry; human; fig s1
Daubon T, Spuul P, Alonso F, Fremaux I, Genot E. VEGF-A stimulates podosome-mediated collagen-IV proteolysis in microvascular endothelial cells. J Cell Sci. 2016;129:2586-98 pubmed publisher
  • western blot; human; loading ...; fig s15a
Bowser J, Blackburn M, Shipley G, Molina J, Dunner K, Broaddus R. Loss of CD73-mediated actin polymerization promotes endometrial tumor progression. J Clin Invest. 2016;126:220-38 pubmed publisher
  • immunocytochemistry; human; 1:50; fig s5i
  • western blot; human; 1:50; fig s5g
Priya R, Gomez G, Budnar S, Verma S, Cox H, Hamilton N, et al. Feedback regulation through myosin II confers robustness on RhoA signalling at E-cadherin junctions. Nat Cell Biol. 2015;17:1282-93 pubmed publisher
  • immunocytochemistry; dogs; fig 8b
  • western blot; dogs; fig 8a
Van Itallie C, Tietgens A, Krystofiak E, Kachar B, Anderson J. A complex of ZO-1 and the BAR-domain protein TOCA-1 regulates actin assembly at the tight junction. Mol Biol Cell. 2015;26:2769-87 pubmed publisher
  • western blot; human; fig s9
Westcott J, Prechtl A, Maine E, Dang T, Esparza M, Sun H, et al. An epigenetically distinct breast cancer cell subpopulation promotes collective invasion. J Clin Invest. 2015;125:1927-43 pubmed publisher
  • western blot; human; loading ...; fig s3b
Ferru Clément R, Fresquet F, Norez C, Métayé T, Becq F, Kitzis A, et al. Involvement of the Cdc42 pathway in CFTR post-translational turnover and in its plasma membrane stability in airway epithelial cells. PLoS ONE. 2015;10:e0118943 pubmed publisher
  • western blot; human; fig 1
Gianfelice A, Le P, Rigano L, Saila S, Dowd G, McDivitt T, et al. Host endoplasmic reticulum COPII proteins control cell-to-cell spread of the bacterial pathogen Listeria monocytogenes. Cell Microbiol. 2015;17:876-92 pubmed publisher
  • western blot; rat; 1:500; fig 4
Kim A, Zamora Martinez E, Edwards S, Mandyam C. Structural reorganization of pyramidal neurons in the medial prefrontal cortex of alcohol dependent rats is associated with altered glial plasticity. Brain Struct Funct. 2015;220:1705-20 pubmed publisher
  • western blot; mouse; 1:1000
Ziegler A, Chidambaram S, Forbes B, Wood T, Levison S. Insulin-like growth factor-II (IGF-II) and IGF-II analogs with enhanced insulin receptor-a binding affinity promote neural stem cell expansion. J Biol Chem. 2014;289:4626-33 pubmed publisher
Hoffmann C, Mao X, Brown Clay J, Moreau F, Al Absi A, Wurzer H, et al. Hypoxia promotes breast cancer cell invasion through HIF-1?-mediated up-regulation of the invadopodial actin bundling protein CSRP2. Sci Rep. 2018;8:10191 pubmed publisher
Bonfim Melo A, Ferreira d, Mortara R. Rac1/WAVE2 and Cdc42/N-WASP Participation in Actin-Dependent Host Cell Invasion by Extracellular Amastigotes of Trypanosoma cruzi. Front Microbiol. 2018;9:360 pubmed publisher
Chapnik E, Rivkin N, Mildner A, Beck G, Pasvolsky R, Metzl Raz E, et al. miR-142 orchestrates a network of actin cytoskeleton regulators during megakaryopoiesis. elife. 2014;3:e01964 pubmed publisher
Samuelson D, Konkel M. Serine phosphorylation of cortactin is required for maximal host cell invasion by Campylobacter jejuni. Cell Commun Signal. 2013;11:82 pubmed publisher
Murk K, Blanco Suarez E, Cockbill L, Banks P, Hanley J. The antagonistic modulation of Arp2/3 activity by N-WASP, WAVE2 and PICK1 defines dynamic changes in astrocyte morphology. J Cell Sci. 2013;126:3873-83 pubmed publisher
Jung J, Oh J, Lee D, Lee S, Chung J. Blood type B antigen modulates cell migration through regulating cdc42 expression and activity in HaCaT cells. J Cell Physiol. 2013;228:2243-51 pubmed publisher
Dehapiot B, Carriere V, Carroll J, Halet G. Polarized Cdc42 activation promotes polar body protrusion and asymmetric division in mouse oocytes. Dev Biol. 2013;377:202-12 pubmed publisher
Juin A, Billottet C, Moreau V, Destaing O, Albiges Rizo C, Rosenbaum J, et al. Physiological type I collagen organization induces the formation of a novel class of linear invadosomes. Mol Biol Cell. 2012;23:297-309 pubmed publisher
Rajabian T, Gavicherla B, Heisig M, Müller Altrock S, Goebel W, Gray Owen S, et al. The bacterial virulence factor InlC perturbs apical cell junctions and promotes cell-to-cell spread of Listeria. Nat Cell Biol. 2009;11:1212-8 pubmed publisher
product information
SKU :
4848S
Product-Name :
N-WASP (30D10) Rabbit mAb
Size :
100 ul
Price-(USD) :
235 USD
Species-x-Reactivity :
H, M, R, Hm, Mk, B
Applications :
Immunoprecipitation
Product-Category :
Cytoskeletal Signaling
Shipping-Temp :
AMBIENT
Storage-Temp :
-20°C
Product-Type :
Monoclonal Antibody
MW :
65
Host :
Rabbit
Target :
N-WASP
Primary-Protein :
N-WASP
Alt-Names :
DKFZp779G0847,MGC48327,N-WASP,NWASP,Neural Wiskott-Aldrich syndrome protein,WASL,Wiskott-Aldrich syndrome gene-like,Wiskott-Aldrich syndrome gene-like protein,Wiskott-Aldrich syndrome-like
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
https://www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.