product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
MEK1/2 (L38C12) Mouse mAb
catalog :
4694
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
L38C12
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry
citations: 41
Published Application/Species/Sample/DilutionReference
  • western blot; human; loading ...; fig s3d
Huang J, Wang X, Li B, Shen S, Wang R, Tao H, et al. L-5-hydroxytryptophan promotes antitumor immunity by inhibiting PD-L1 inducible expression. J Immunother Cancer. 2022;10: pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 4b
Grinat J, Kosel F, Goveas N, Kranz A, Alexopoulou D, Rajewsky K, et al. Epigenetic modifier balances Mapk and Wnt signalling in differentiation of goblet and Paneth cells. Life Sci Alliance. 2022;5: pubmed publisher
  • western blot; mouse; loading ...; fig 4c
Li S, Zhu Z, Xue M, Pan X, Tong G, Yi X, et al. The protective effects of fibroblast growth factor 10 against hepatic ischemia-reperfusion injury in mice. Redox Biol. 2021;40:101859 pubmed publisher
  • western blot; mouse; loading ...
Goswami D, Chen D, Yang Y, Gudla P, Columbus J, Worthy K, et al. Membrane interactions of the globular domain and the hypervariable region of KRAS4b define its unique diffusion behavior. elife. 2020;9: pubmed publisher
  • western blot; human; 1:1000; loading ...; fig e6d
Amendola C, Mahaffey J, Parker S, Ahearn I, Chen W, Zhou M, et al. KRAS4A directly regulates hexokinase 1. Nature. 2019;576:482-486 pubmed publisher
  • western blot; human; loading ...; fig 3d
Rushing A, Rushing B, Hoang K, Sanders S, Peloponese J, Polakowski N, et al. HTLV-1 basic leucine zipper factor protects cells from oxidative stress by upregulating expression of Heme Oxygenase I. PLoS Pathog. 2019;15:e1007922 pubmed publisher
  • western blot; human; fig 4b
Neel D, Allegakoen D, Olivas V, Mayekar M, Hemmati G, Chatterjee N, et al. Differential Subcellular Localization Regulates Oncogenic Signaling by ROS1 Kinase Fusion Proteins. Cancer Res. 2019;79:546-556 pubmed publisher
  • western blot; human; loading ...; fig s10b, s10d
  • western blot; mouse; loading ...; fig s10c
Gerber T, Murawala P, Knapp D, Masselink W, Schuez M, Hermann S, et al. Single-cell analysis uncovers convergence of cell identities during axolotl limb regeneration. Science. 2018;362: pubmed publisher
  • western blot; human; fig 3d
Vanshylla K, Bartsch C, Hitzing C, Krümpelmann L, Wienands J, Engels N. Grb2 and GRAP connect the B cell antigen receptor to Erk MAP kinase activation in human B cells. Sci Rep. 2018;8:4244 pubmed publisher
  • western blot; human; loading ...; fig s5o
Schafer S, Viswanathan S, Widjaja A, Lim W, Moreno Moral A, Delaughter D, et al. IL-11 is a crucial determinant of cardiovascular fibrosis. Nature. 2017;552:110-115 pubmed publisher
  • western blot; human; loading ...; fig 6f
Jiang X, Bao Y, Liu H, Kou X, Zhang Z, Sun F, et al. VPS34 stimulation of p62 phosphorylation for cancer progression. Oncogene. 2017;36:6850-6862 pubmed publisher
  • western blot; human; loading ...; fig 4d
Umstead M, Xiong J, Qi Q, Du Y, Fu H. Aurora kinase A interacts with H-Ras and potentiates Ras-MAPK signaling. Oncotarget. 2017;8:28359-28372 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 3b
Xiang J, Yang S, Xin N, Gaertig M, Reeves R, Li S, et al. DYRK1A regulates Hap1-Dcaf7/WDR68 binding with implication for delayed growth in Down syndrome. Proc Natl Acad Sci U S A. 2017;114:E1224-E1233 pubmed publisher
  • western blot; mouse; loading ...; fig 2a
Kidger A, Rushworth L, Stellzig J, Davidson J, Bryant C, Bayley C, et al. Dual-specificity phosphatase 5 controls the localized inhibition, propagation, and transforming potential of ERK signaling. Proc Natl Acad Sci U S A. 2017;114:E317-E326 pubmed publisher
  • western blot; human; loading ...; fig 1b
Lagares Tena L, García Monclús S, López Alemany R, Almacellas Rabaiget O, Huertas Martínez J, Sáinz Jaspeado M, et al. Caveolin-1 promotes Ewing sarcoma metastasis regulating MMP-9 expression through MAPK/ERK pathway. Oncotarget. 2016;7:56889-56903 pubmed publisher
  • immunocytochemistry; human; 1:25; loading ...; fig 4a
Dai H, Chen H, Liu W, You Y, Tan J, Yang A, et al. Effects of Raf kinase inhibitor protein expression on pancreatic cancer cell growth and motility: an in vivo and in vitro study. J Cancer Res Clin Oncol. 2016;142:2107-17 pubmed publisher
  • western blot; human; 1:1000; fig 5e
Zhang Y, Velez Delgado A, Mathew E, Li D, Mendez F, Flannagan K, et al. Myeloid cells are required for PD-1/PD-L1 checkpoint activation and the establishment of an immunosuppressive environment in pancreatic cancer. Gut. 2017;66:124-136 pubmed publisher
  • western blot; human; fig 2a
Kemper K, Krijgsman O, Kong X, Cornelissen Steijger P, Shahrabi A, Weeber F, et al. BRAF(V600E) Kinase Domain Duplication Identified in Therapy-Refractory Melanoma Patient-Derived Xenografts. Cell Rep. 2016;16:263-277 pubmed publisher
  • western blot; human; 1:1000; fig 1
Simpson D, Lemonie N, Morgan D, Gaddameedhi S, Kaufmann W. Oncogenic BRAF(V600E) Induces Clastogenesis and UVB Hypersensitivity. Cancers (Basel). 2015;7:1072-90 pubmed publisher
  • western blot; human; fig s3
Vogel C, Smit M, Maddalo G, Possik P, Sparidans R, van der Burg S, et al. Cooperative induction of apoptosis in NRAS mutant melanoma by inhibition of MEK and ROCK. Pigment Cell Melanoma Res. 2015;28:307-17 pubmed publisher
  • western blot; mouse; fig s2
Guillaumond F, Bidaut G, Ouaissi M, Servais S, Gouirand V, Olivares O, et al. Cholesterol uptake disruption, in association with chemotherapy, is a promising combined metabolic therapy for pancreatic adenocarcinoma. Proc Natl Acad Sci U S A. 2015;112:2473-8 pubmed publisher
  • western blot; human; fig 6
Papadakis A, Sun C, Knijnenburg T, Xue Y, Grernrum W, Hölzel M, et al. SMARCE1 suppresses EGFR expression and controls responses to MET and ALK inhibitors in lung cancer. Cell Res. 2015;25:445-58 pubmed publisher
  • western blot; mouse
Park E, Kim B, Lee E, Chang E, Kim D, Choi S, et al. Targeting of receptor for advanced glycation end products suppresses cyst growth in polycystic kidney disease. J Biol Chem. 2014;289:9254-62 pubmed publisher
  • western blot; human
Choi J, Landrette S, Wang T, Evans P, Bacchiocchi A, Bjornson R, et al. Identification of PLX4032-resistance mechanisms and implications for novel RAF inhibitors. Pigment Cell Melanoma Res. 2014;27:253-62 pubmed publisher
Tan M, Sng M, Cheng H, Low Z, Leong B, Chua D, et al. Deficiency in fibroblast PPARβ/δ reduces nonmelanoma skin cancers in mice. Cell Death Differ. 2020;27:2668-2680 pubmed publisher
Jiu Y, Peranen J, Schaible N, Cheng F, Eriksson J, Krishnan R, et al. Vimentin intermediate filaments control actin stress fiber assembly through GEF-H1 and RhoA. J Cell Sci. 2017;130:892-902 pubmed publisher
Zhou M, Wiener H, Su W, Zhou Y, Liot C, Ahearn I, et al. VPS35 binds farnesylated N-Ras in the cytosol to regulate N-Ras trafficking. J Cell Biol. 2016;214:445-58 pubmed publisher
Billing A, Ben Hamidane H, Dib S, Cotton R, Bhagwat A, Kumar P, et al. Comprehensive transcriptomic and proteomic characterization of human mesenchymal stem cells reveals source specific cellular markers. Sci Rep. 2016;6:21507 pubmed publisher
Jang J, Lee A, Li J, Liyanage H, Yang Y, Guo L, et al. Common Oncogene Mutations and Novel SND1-BRAF Transcript Fusion in Lung Adenocarcinoma from Never Smokers. Sci Rep. 2015;5:9755 pubmed publisher
Blum D, Labarge S. Registered report: Tumour micro-environment elicits innate resistance to RAF inhibitors through HGF secretion. elife. 2014;3: pubmed publisher
Carlino M, Fung C, Shahheydari H, Todd J, Boyd S, Irvine M, et al. Preexisting MEK1P124 mutations diminish response to BRAF inhibitors in metastatic melanoma patients. Clin Cancer Res. 2015;21:98-105 pubmed publisher
Brobeil A, Graf M, Eiber M, Wimmer M. Interaction of PTPIP51 with Tubulin, CGI-99 and Nuf2 During Cell Cycle Progression. Biomolecules. 2012;2:122-42 pubmed publisher
Hughes A, Spelke D, Xu Z, Kang C, Schaffer D, Herr A. Single-cell western blotting. Nat Methods. 2014;11:749-55 pubmed publisher
Rizos H, Menzies A, Pupo G, Carlino M, Fung C, Hyman J, et al. BRAF inhibitor resistance mechanisms in metastatic melanoma: spectrum and clinical impact. Clin Cancer Res. 2014;20:1965-77 pubmed publisher
Mayes D, Rizvi T, Titus Mitchell H, Oberst R, Ciraolo G, Vorhees C, et al. Nf1 loss and Ras hyperactivation in oligodendrocytes induce NOS-driven defects in myelin and vasculature. Cell Rep. 2013;4:1197-212 pubmed publisher
Carnevale J, Ross L, Puissant A, Banerji V, Stone R, DeAngelo D, et al. SYK regulates mTOR signaling in AML. Leukemia. 2013;27:2118-28 pubmed publisher
Lin S, Hoffmann K, Xiao Z, Jin N, Galli U, Mohr E, et al. MEK inhibition induced downregulation of MRP1 and MRP3 expression in experimental hepatocellular carcinoma. Cancer Cell Int. 2013;13:3 pubmed publisher
Lee J, Huang S, Lee M, Lee S, Ryu M, Kim S, et al. Dual specificity phosphatase 6 as a predictor of invasiveness in papillary thyroid cancer. Eur J Endocrinol. 2012;167:93-101 pubmed publisher
Satoh T, Smith A, Sarde A, Lu H, Mian S, Mian S, et al. B-RAF mutant alleles associated with Langerhans cell histiocytosis, a granulomatous pediatric disease. PLoS ONE. 2012;7:e33891 pubmed publisher
Falco A, Festa M, Basile A, Rosati A, Pascale M, Florenzano F, et al. BAG3 controls angiogenesis through regulation of ERK phosphorylation. Oncogene. 2012;31:5153-61 pubmed publisher
Ahmad I, Patel R, Liu Y, Singh L, Taketo M, Wu X, et al. Ras mutation cooperates with ?-catenin activation to drive bladder tumourigenesis. Cell Death Dis. 2011;2:e124 pubmed publisher
product information
SKU :
4694S
Product-Name :
MEK1/2 (L38C12) Mouse mAb
Size :
100 ul
Price-(USD) :
235 USD
Species-x-Reactivity :
H, M, R, Mk
Applications :
Flow cytometry
Product-Category :
MAP Kinase Signaling
Shipping-Temp :
AMBIENT
Storage-Temp :
-20°C
Product-Type :
Monoclonal Antibody
MW :
45
Host :
Mouse
Target :
MEK1/MEK2
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
https://www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.