product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
Di-Methyl-Histone H3 (Lys9) (D85B4) XP® Rabbit mAb
catalog :
4658
clonality :
monoclonal
host :
rabbit
conjugate :
nonconjugated
clone name :
D85B4
reactivity :
human, mouse, rat, cow, zebrafish
application :
western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation
more info or order :
Cell Signaling Technology product webpage
citations: 15
Published Application/Species/Sample/DilutionReference
  • western blot; human; 1:1000; tbl s6
Mair B, Konopka T, Kerzendorfer C, Sleiman K, Salic S, Serra V, et al. Gain- and Loss-of-Function Mutations in the Breast Cancer Gene GATA3 Result in Differential Drug Sensitivity. PLoS Genet. 2016;12:e1006279 pubmed publisher
  • western blot; human; 1:1000; fig 1
Grinshtein N, Rioseco C, Marcellus R, UEHLING D, Aman A, Lun X, et al. Small molecule epigenetic screen identifies novel EZH2 and HDAC inhibitors that target glioblastoma brain tumor-initiating cells. Oncotarget. 2016;7:59360-59376 pubmed publisher
  • western blot; mouse; 1:1000; fig 3
Hakim S, Dyson J, Feeney S, Davies E, Sriratana A, Koenig M, et al. Inpp5e suppresses polycystic kidney disease via inhibition of PI3K/Akt-dependent mTORC1 signaling. Hum Mol Genet. 2016;25:2295-2313 pubmed
  • western blot; human; 1:1000; loading ...; fig 12a
Perez R, Shen H, Duan L, Kim R, Kim T, Park N, et al. Modeling the Etiology of p53-mutated Cancer Cells. J Biol Chem. 2016;291:10131-47 pubmed publisher
  • western blot; human; loading ...; fig 2g
Okoye Okafor U, Bartholdy B, Cartier J, Gao E, Pietrak B, Rendina A, et al. New IDH1 mutant inhibitors for treatment of acute myeloid leukemia. Nat Chem Biol. 2015;11:878-86 pubmed publisher
  • immunocytochemistry; mouse
  • western blot; mouse
Yang J, Kaur K, Ong L, Eisenberg C, Eisenberg L. Inhibition of G9a Histone Methyltransferase Converts Bone Marrow Mesenchymal Stem Cells to Cardiac Competent Progenitors. Stem Cells Int. 2015;2015:270428 pubmed publisher
  • immunocytochemistry; mouse; 1:100
Huang X, Shen M, Wang L, Yu F, Wu W, Liu H. Effects of tributyltin chloride on developing mouse oocytes and preimplantation embryos. Microsc Microanal. 2015;21:358-67 pubmed publisher
  • western blot; human; fig 6
Hendriks I, Treffers L, Verlaan de Vries M, Olsen J, Vertegaal A. SUMO-2 Orchestrates Chromatin Modifiers in Response to DNA Damage. Cell Rep. 2015;: pubmed publisher
  • western blot; human
Elhammali A, Ippolito J, Collins L, Crowley J, Marasa J, Piwnica Worms D. A high-throughput fluorimetric assay for 2-hydroxyglutarate identifies Zaprinast as a glutaminase inhibitor. Cancer Discov. 2014;4:828-39 pubmed publisher
  • chromatin immunoprecipitation; mouse
  • western blot; mouse; 1:1000
Subbanna S, Nagre N, Shivakumar M, Umapathy N, Psychoyos D, Basavarajappa B. Ethanol induced acetylation of histone at G9a exon1 and G9a-mediated histone H3 dimethylation leads to neurodegeneration in neonatal mice. Neuroscience. 2014;258:422-32 pubmed publisher
  • western blot; human
Lauffer B, Mintzer R, Fong R, Mukund S, Tam C, Zilberleyb I, et al. Histone deacetylase (HDAC) inhibitor kinetic rate constants correlate with cellular histone acetylation but not transcription and cell viability. J Biol Chem. 2013;288:26926-43 pubmed publisher
  • western blot; mouse; 1:1000; fig 4c
Subbanna S, Shivakumar M, Umapathy N, Saito M, Mohan P, Kumar A, et al. G9a-mediated histone methylation regulates ethanol-induced neurodegeneration in the neonatal mouse brain. Neurobiol Dis. 2013;54:475-85 pubmed publisher
Wang Y, Zhang J, Su Y, Shen Y, Jiang D, Hou Y, et al. G9a regulates breast cancer growth by modulating iron homeostasis through the repression of ferroxidase hephaestin. Nat Commun. 2017;8:274 pubmed publisher
Lai Z, Moravcová S, Canitrot Y, Andrzejewski L, Walshe D, Rea S. Msl2 is a novel component of the vertebrate DNA damage response. PLoS ONE. 2013;8:e68549 pubmed publisher
Ohhata T, Senner C, Hemberger M, Wutz A. Lineage-specific function of the noncoding Tsix RNA for Xist repression and Xi reactivation in mice. Genes Dev. 2011;25:1702-15 pubmed publisher
product information
SKU :
4658P
Product-Name :
Di-Methyl-Histone H3 (Lys9) (D85B4) XP® Rabbit mAb
Size :
40 ul
Price-(USD) :
135 USD
Species-x-Reactivity :
H, M, R, Mk, (Dm, X, Z, B, Pg, Sc, Ce)
Applications :
Chromatin Immunoprecipitation
Product-Category :
Chromatin Regulation / Nuclear Function
Shipping-Temp :
AMBIENT
Storage-Temp :
-20°C
Product-Type :
Monoclonal Antibody
MW :
17
Host :
Rabbit
Target :
H3 (Lys10) dimethylate
Primary-Protein :
H3
Alt-Names :
H3 histone family, member A,H3/A,H31,H3FA,H3FB,H3FC,H3FD,H3FF,H3FH,H3FI,H3FJ,H3FK,H3FL,HIST1H3A,HIST1H3B,HIST1H3C,HIST1H3D,HIST1H3E,HIST1H3F,HIST1H3G,HIST1H3H,HIST1H3I,HIST1H3J,Histone H3.1,Histone H3/a,Histone H3/b,Histone H3/c,Histone H3/d,Histone H3/f,Histone H3/h,Histone H3/i,Histone H3/j,Histone H3/k,Histone H3/l,histone 1, H3a,histone cluster 1, H3a
more info or order :
Cell Signaling Technology product webpage
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.