product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
β-TrCP (D13F10) Rabbit mAb
catalog :
4394
clonality :
monoclonal
host :
rabbit
conjugate :
nonconjugated
clone name :
D13F10
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, western blot knockout validation
more info or order :
Cell Signaling Technology product webpage
citations: 33
Published Application/Species/Sample/DilutionReference
  • western blot; human; loading ...; fig 1d
  • western blot knockout validation; mouse; loading ...; fig 8e
Shimizu K, Fukushima H, Ogura K, Lien E, Nihira N, Zhang J, et al. The SCF?-TRCP E3 ubiquitin ligase complex targets Lipin1 for ubiquitination and degradation to promote hepatic lipogenesis. Sci Signal. 2017;10: pubmed publisher
  • western blot; human; loading ...; fig 5e
Zhu Y, Qu C, Hong X, Jia Y, Lin M, Luo Y, et al. Trabid inhibits hepatocellular carcinoma growth and metastasis by cleaving RNF8-induced K63 ubiquitination of Twist1. Cell Death Differ. 2019;26:306-320 pubmed publisher
  • immunoprecipitation; human; loading ...; fig 3c
Li N, Truong S, Nouri M, Moore J, Al Nakouzi N, Lubik A, et al. Non-canonical activation of hedgehog in prostate cancer cells mediated by the interaction of transcriptionally active androgen receptor proteins with Gli3. Oncogene. 2018;37:2313-2325 pubmed publisher
  • western blot; human; loading ...; fig 4d
Choi E, Jung B, Lee S, Yoo H, Shin E, Ko H, et al. A clinical drug library screen identifies clobetasol propionate as an NRF2 inhibitor with potential therapeutic efficacy in KEAP1 mutant lung cancer. Oncogene. 2017;36:5285-5295 pubmed publisher
  • western blot; human; loading ...; fig 5c
Li P, Wang Y, Mao X, Jiang Y, Liu J, Li J, et al. CRB3 downregulation confers breast cancer stem cell traits through TAZ/?-catenin. Oncogenesis. 2017;6:e322 pubmed publisher
  • western blot; human; loading ...; fig 3d
Zhao D, Lu X, Wang G, Lan Z, Liao W, Li J, et al. Synthetic essentiality of chromatin remodelling factor CHD1 in PTEN-deficient cancer. Nature. 2017;542:484-488 pubmed publisher
  • immunoprecipitation; human; loading ...; fig 1a
  • immunohistochemistry; human; 1:500; loading ...; fig 6b
  • western blot; human; 1:1000; loading ...; fig 6b
Xu J, Zhou W, Yang F, Chen G, Li H, Zhao Y, et al. The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor. Nat Commun. 2017;8:14002 pubmed publisher
  • western blot; human; loading ...; fig 2e
Nagashima K, Fukushima H, Shimizu K, Yamada A, Hidaka M, Hasumi H, et al. Nutrient-induced FNIP degradation by SCFβ-TRCP regulates FLCN complex localization and promotes renal cancer progression. Oncotarget. 2017;8:9947-9960 pubmed publisher
  • western blot; human; loading ...; fig 4f
Matsumoto Y, La Rose J, Kent O, Wagner M, Narimatsu M, Levy A, et al. Reciprocal stabilization of ABL and TAZ regulates osteoblastogenesis through transcription factor RUNX2. J Clin Invest. 2016;126:4482-4496 pubmed publisher
  • western blot; human; loading ...; fig 1e
Ding S, Mooney N, Li B, Kelly M, Feng N, Loktev A, et al. Comparative Proteomics Reveals Strain-Specific β-TrCP Degradation via Rotavirus NSP1 Hijacking a Host Cullin-3-Rbx1 Complex. PLoS Pathog. 2016;12:e1005929 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig s6b
Li C, Lim S, Xia W, Lee H, Chan L, Kuo C, et al. Glycosylation and stabilization of programmed death ligand-1 suppresses T-cell activity. Nat Commun. 2016;7:12632 pubmed publisher
  • western blot; human; loading ...; fig 7b
Scott D, Rhee D, Duda D, Kelsall I, Olszewski J, Paulo J, et al. Two Distinct Types of E3 Ligases Work in Unison to Regulate Substrate Ubiquitylation. Cell. 2016;166:1198-1214.e24 pubmed publisher
  • immunoprecipitation; mouse; loading ...; fig 3f
  • western blot; mouse; loading ...; fig 3g
Zhou Y, Xu H, Ding Y, Lu Q, Zou M, Song P. AMPK?1 deletion in fibroblasts promotes tumorigenesis in athymic nude mice by p52-mediated elevation of erythropoietin and CDK2. Oncotarget. 2016;7:53654-53667 pubmed publisher
  • immunoprecipitation; human
  • western blot; human
Tang X, Chen X, Xu Y, Qiao Y, Zhang X, Wang Y, et al. CD166 positively regulates MCAM via inhibition to ubiquitin E3 ligases Smurf1 and βTrCP through PI3K/AKT and c-Raf/MEK/ERK signaling in Bel-7402 hepatocellular carcinoma cells. Cell Signal. 2015;27:1694-702 pubmed publisher
  • western blot; human; fig 1
Yi Y, Kang H, Bae E, Oh S, Seong Y, Bae I. β-TrCP1 degradation is a novel action mechanism of PI3K/mTOR inhibitors in triple-negative breast cancer cells. Exp Mol Med. 2015;47:e143 pubmed publisher
  • western blot; human; 1:1000; fig s5
Xue J, Chen Y, Wu Y, Wang Z, Zhou A, Zhang S, et al. Tumour suppressor TRIM33 targets nuclear β-catenin degradation. Nat Commun. 2015;6:6156 pubmed publisher
  • western blot; human; 1:500; fig 2
Pagan J, Marzio A, Jones M, Saraf A, Jallepalli P, Florens L, et al. Degradation of Cep68 and PCNT cleavage mediate Cep215 removal from the PCM to allow centriole separation, disengagement and licensing. Nat Cell Biol. 2015;17:31-43 pubmed publisher
  • western blot; human
Kim T, Siesser P, Rossman K, Goldfarb D, Mackinnon K, Yan F, et al. Substrate trapping proteomics reveals targets of the βTrCP2/FBXW11 ubiquitin ligase. Mol Cell Biol. 2015;35:167-81 pubmed publisher
  • immunocytochemistry; human
  • western blot; human
Xu S, Tong M, Huang J, Zhang Y, Qiao Y, Weng W, et al. TRIB2 inhibits Wnt/β-Catenin/TCF4 signaling through its associated ubiquitin E3 ligases, β-TrCP, COP1 and Smurf1, in liver cancer cells. FEBS Lett. 2014;588:4334-41 pubmed publisher
  • western blot; human; loading ...; fig 2b
Chen Z, Liu B, Tang N, Xu Y, Ye X, Li Z, et al. FBXL5-mediated degradation of single-stranded DNA-binding protein hSSB1 controls DNA damage response. Nucleic Acids Res. 2014;42:11560-9 pubmed publisher
  • western blot; human
Ma L, Pan Q, Sun F, Yu Y, Wang J. Cluster of differentiation 166 (CD166) regulates cluster of differentiation (CD44) via NF-?B in liver cancer cell line Bel-7402. Biochem Biophys Res Commun. 2014;451:334-8 pubmed publisher
  • western blot; human; fig 5
Azzolin L, Panciera T, Soligo S, Enzo E, Bicciato S, Dupont S, et al. YAP/TAZ incorporation in the ?-catenin destruction complex orchestrates the Wnt response. Cell. 2014;158:157-70 pubmed publisher
  • immunoprecipitation; human; fig 1
  • western blot; human; fig 1
Zhu G, Fan Z, Ding M, Mu L, Liang J, Ding Y, et al. DNA damage induces the accumulation of Tiam1 by blocking ?-TrCP-dependent degradation. J Biol Chem. 2014;289:15482-94 pubmed publisher
Jiang Z, Peng T, Qian H, Lu C, Qiu F, Zhang S. DNA damage-induced activation of ATM promotes β-TRCP-mediated ARID1A ubiquitination and destruction in gastric cancer cells. Cancer Cell Int. 2019;19:162 pubmed publisher
Bunda S, Heir P, Metcalf J, Li A, Agnihotri S, Pusch S, et al. CIC protein instability contributes to tumorigenesis in glioblastoma. Nat Commun. 2019;10:661 pubmed publisher
White K, Grillo Hill B, Esquivel M, Peralta J, Bui V, Chire I, et al. β-Catenin is a pH sensor with decreased stability at higher intracellular pH. J Cell Biol. 2018;217:3965-3976 pubmed publisher
Sekine H, Okazaki K, Kato K, Alam M, Shima H, Katsuoka F, et al. O-GlcNAcylation Signal Mediates Proteasome Inhibitor Resistance in Cancer Cells by Stabilizing NRF1. Mol Cell Biol. 2018;38: pubmed publisher
Zhao L, Wang X, Yu Y, Deng L, Chen L, Peng X, et al. OTUB1 protein suppresses mTOR complex 1 (mTORC1) activity by deubiquitinating the mTORC1 inhibitor DEPTOR. J Biol Chem. 2018;293:4883-4892 pubmed publisher
Diamantopoulou Z, White G, Fadlullah M, Dreger M, Pickering K, Maltas J, et al. TIAM1 Antagonizes TAZ/YAP Both in the Destruction Complex in the Cytoplasm and in the Nucleus to Inhibit Invasion of Intestinal Epithelial Cells. Cancer Cell. 2017;31:621-634.e6 pubmed publisher
Shi M, Cho H, Inn K, Yang A, Zhao Z, Liang Q, et al. Negative regulation of NF-κB activity by brain-specific TRIpartite Motif protein 9. Nat Commun. 2014;5:4820 pubmed publisher
Wu X, Fukushima H, North B, Nagaoka Y, Nagashima K, Deng F, et al. SCF?-TRCP regulates osteoclastogenesis via promoting CYLD ubiquitination. Oncotarget. 2014;5:4211-21 pubmed
Cui W, Xiao N, Xiao H, Zhou H, Yu M, Gu J, et al. ?-TrCP-mediated IRAK1 degradation releases TAK1-TRAF6 from the membrane to the cytosol for TAK1-dependent NF-?B activation. Mol Cell Biol. 2012;32:3990-4000 pubmed publisher
Shaik S, Nucera C, Inuzuka H, Gao D, Garnaas M, Frechette G, et al. SCF(?-TRCP) suppresses angiogenesis and thyroid cancer cell migration by promoting ubiquitination and destruction of VEGF receptor 2. J Exp Med. 2012;209:1289-307 pubmed publisher
product information
SKU :
4394S
Product-Name :
β-TrCP (D13F10) Rabbit mAb
Size :
100 ul
Price-(USD) :
235 USD
Species-x-Reactivity :
H, M, R, Mk
Applications :
Immunoprecipitation
Product-Category :
Ubiquitin and Ubiquitin-like proteins
Shipping-Temp :
AMBIENT
Storage-Temp :
-20°C
Product-Type :
Monoclonal Antibody
MW :
62
Host :
Rabbit
Target :
beta-Trcp
Primary-Protein :
BTRC
Alt-Names :
BETA-TRCP,BTRC,BTRCP,E3RSIkappaB,F-box and WD repeats protein beta-TrCP,F-box/WD repeat-containing protein 1A,FBW1A,FBXW1,FBXW1A,FWD1,MGC4643,bTrCP1,beta-TrCP1,beta-transducin repeat containing,betaTrCP,pIkappaBalpha-E3 receptor subunit
more info or order :
Cell Signaling Technology product webpage
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
https://www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.